array comparative genomic hybridization characterization of human pluripotent stem cells
Atlas of Human Pluripotent Stem Cells doc
Ngày tải lên :
22/03/2014, 09:20
... culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube baby ... summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The ... translational research in the field of embryonic and induced pluripotent stem cells It commences with practical aspects of the derivation and growth of human embryonic stem cells from inner cell mass...
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báo cáo khoa học: "Genomic profiling of plasmablastic lymphoma using array comparative genomic hybridization (aCGH): revealing significant overlapping genomic lesions with diffuse large B-cell lymphoma" ppt
Ngày tải lên :
10/08/2014, 22:20
... (Figure 1B) FISH performed in subsets of cases including cases of PL and cases of myeloma showed gain of this region in of PL cases but in none of myeloma cases Of note, our previous immuno- http://www.jhoonline.org/content/2/1/47 ... Boards of participating institutions One H&E section of each case was reviewed to confirm that more than 80% of cells were neoplastic cells DNA was then extracted from the consecutive section of ... would be of great interest to further correlate the array CGH findings with gene expression profiling of these types of lymphoma's to further clarify the relationship among these types of lymphoma...
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Báo cáo hóa học: " Chromosomal 16p microdeletion in Rubinstein-Taybi syndrome detected by oligonucleotide-based array comparative genomic hybridization: a case report" doc
Ngày tải lên :
21/06/2014, 19:20
... undetected in unexplained syndromic conditions of young children This case report demonstrates the ability of array- based comparative genomic hybridization to offer a genome-wide analysis at high resolution ... findings of a normal karyotype This necessitates the adoption of a more sensitive method to detect the occurrence of any submicroscopic chromosomal aberration Microarray-based comparative genomic hybridization ... oligonucleotide-based array comparative genomic hybridization: a case report Mohd Fadly Md Ahid1*, Azli Ismail1, Thong Meow Keong2, Narazah Mohd Yusoff3, Zubaidah Zakaria1* Unit of Hematology, Cancer...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
Ngày tải lên :
11/08/2014, 12:21
... Figure The combination of stem cell models and epigenomics in studies of the role of non-coding mutations in human disease Epigenomic analyses of cells derived through in vitro stem cell differentiation ... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine ... stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3;...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
Ngày tải lên :
31/10/2012, 16:57
... xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes by combinatorial multi-fluor ... Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
Ngày tải lên :
18/06/2014, 15:20
... 13 The expression of miR-92 has been reported in human embryonic stem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine ... The tested adult cells were Human Microvascular Endothelial Cells (HMVEC), Human Umbilical Vein Endothelial Cells (HUVEC), Umbilical Artery Smooth Muscle Cells (UASMC), Normal human astrocytes ... undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational Medicine 2009, 7:20...
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Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf
Ngày tải lên :
09/08/2014, 10:23
... number of cells was determined spectrophotometrically entiation of human mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem ... Lecanda F, Avioli LV, Cheng SL: Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2 ... cells/ cm2 of human MSCs were seeded in BM in each well of 24-well plates (Becton Dickinson Labware, Franklin Lakes, NJ, USA) Each experimental condition was repeated in quadruplicate On day of...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
Ngày tải lên :
09/08/2014, 14:22
... differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem ... mesenchymal stem cells for osteoarthritis therapy Nat Clin Pract Rheumatol 2008, 4:371-380 Estes BT, Wu AW, Guilak F: Potent induction of chondrocytic differentiation of human adipose-derived adult stem ... arcades of Benninghoff [29] and by the appearance of hypertrophic cells Analyses of lacunae surface areas in the different growth plate zones revealed mean lacunae surface areas ± SD of 100.8...
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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
Ngày tải lên :
10/08/2014, 05:21
... differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved stem cells ... differentiation of human mesenchymal stem cells into insulin producing cells J Biomed Mater Res A 2008, 86(4):1097-1105 Chao KC, Chao KF, Fu YS, Liu SH: Islet-like clusters derived from mesenchymal stem cells ... Fishman B, Ziskind A, Shulman M, Itskovitz-Eldor J: Differentiation of human embryonic stem cells into insulin-producing clusters Stem Cells 2004, 22(3):265-274 Chang CF, Hsu KH, Chiou SH, Ho LL, Fu...
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Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells
Ngày tải lên :
09/09/2015, 10:07
... NANOFIBERS FOR OSTEOGENIC DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS NGUYEN THI HIEN LUONG (B.Eng., Ho Chi Minh city University of Technology, Vietnam) A THESIS SUBMITTED FOR THE DEGREE OF ... 43 2.3 Stem cells 51 2.3.1 Definition and classification 51 2.3.2 Stem cells in tissue engineering 53 2.3.3 Mesenchymal stem cells 54 v 2.4 Stem cell ... Table 2.9 Classification of stem cells [23-30] 51 Table 2.10 Advantages and disadvantages of fabrication methods of nanofibers 79 Table 2.11 Clinical trials using nanofibers 88 Table...
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Screen of nuclear receptors for the enhanced and alternative generation of induced pluripotent stem cells
Ngày tải lên :
09/09/2015, 18:56
... the process of pluripotential reprogramming in which the lineage restriction of differentiated cells is undone The resultant cells, known colloquially as induced pluripotent stem cells (iPSCs), ... akin to pluripotent embryonic stem cells (ESCs) and pluripotent cells of the early embryo, thus gaining their characteristic developmental potential and other characteristics diagnostic of pluripotent ... SCREEN OF NUCLEAR RECEPTORS FOR THE ENHANCED AND ALTERNATIVE GENERATION OF INDUCED PLURIPOTENT STEM CELLS DOMINIC HENG JIAN CHIEN (B.Sc (Hons), NTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
Ngày tải lên :
11/09/2015, 09:16
... differentiation of human mesenchymal stem cells Exp Cell Res (1):22-37 101 Salasznyk RM, Klees RF, Boskey A, et al 2007;Activation of FAK is necessary for the osteogenic differentiation of human mesenchymal ... 1991;Expression of modified bFGF cDNAs in mammalian cells Ann N Y Acad Sci :149-160 120 Kolf CM, Cho E, Tuan RS 2007;Mesenchymal stromal cells Biology of adult mesenchymal stem cells: regulation of niche, ... differentiation of rat bone marrow-derived mesenchymal stem cells J Bone Miner Res (10):16061614 59 Matsubara T, Tsutsumi S, Pan H, et al 2004;A new technique to expand human mesenchymal stem cells using...
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Baculovirus mediated genetic modification of human embryonic stem cells
Ngày tải lên :
11/09/2015, 14:38
... specific diseases in the course of ex vivo gene therapy 1.1.1 Human Embryonic Stem Cells Human embryonic stem (hES) cells are cells derived from the inner cell mass of human blastocysts The hES cell ... MODIFICATION OF HUMAN EMBRYONIC STEM CELLS DU JUAN (B Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES NATIONAL UNIVERSITY OF SINGAPORE AND INSTITUTE OF ... BACULOVIRAL TRANSDUCTION OF HUMAN EMBRYONIC STEM CELLS 11 2.1 Introduction hES cells hold great potential as a system for the study of lineage specification and as an unlimited source of cells for the regenerative...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
Ngày tải lên :
16/09/2015, 15:55
... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of pluripotent ... Adult stem cells Adult stem cells also known as somatic stem cells can be found in diverse tissues and organs The most common adult stem cells are the hematopoietic stem cells, mesechymal stem cells ... of cells make up the human body viz., germ cells, somatic cells and stem cells Somatic cells include the bulk of the cells that make up the human adult and each of these cells in their differentiated...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
Ngày tải lên :
04/10/2015, 16:03
... culture of ICM cells with stem cell-like morphology from human blastocysts (Fig 1.1A) though cultures failed beyond passages The first long-term culture (4-5 months) of human embryonic stem cells ... analysis of developmental events was made possible with the isolation of embryonic stem cells Extensive investigation of the properties and capabilities of these cells has led to the development of ... in the mouse vii LIST OF FIGURES Figure 1.1 Embryonic origin of human embryonic stem cells and their in vitro characterisation Figure 1.2 Lineage tree of embryo-derived cells and cell lines Figure...
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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
Ngày tải lên :
09/10/2015, 11:24
... marrow (mesenchymal stem cells, hematopoietic stem cells) and (2) embryonic stem and embryonic germ cells derived from discarded human embryos and germ line stem cells Adult stem cells can be derived ... 1.4 Stem cells in bone repair 13 1.5 Human Embryonic stem cells 15 1.5.1 Derivation of HES cells 15 1.5.2 Expansion and maintenance of pluripotency of HES cells ... and pluripotent stem cells that can give rise to representative of all three germ layers Two important sources of stem cells available for regenerative therapy include (1) the adult stem cells...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
Ngày tải lên :
16/10/2015, 11:58
... embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription factors in human ES cells 1.1.3 Induced pluripotent stem cells 1.1.4 Human ... Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of embryos of blastocyst ... studies of LIN28 19 20 Objective of the current study Page II Materials and methods 22 2.1 Culture of human ES cell line 22 2.1.1 Preparation of feeder cells 22 2.1.2 Maintenance of human ES cells...
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Báo cáo y học: " Ring chromosome 13 syndrome characterized by high resolution array based comparative genomic hybridization in patient with 47, XYY syndrome: a case report." doc
Ngày tải lên :
11/08/2014, 00:22
... The median absolute pair-wise difference (MAPD) of each chip was used as a quality assessment Page of of the array data The median MAPD of this array was 0.26, which met the quality control criteria ... [7] Recently, the advent of microarray-based comparative genomic hybridization (aCGH) technology permits the simultaneous rapid high resolution genome analysis and mapping of DNA sequences [8,9] ... 2000, 91:227-230 Kirchhoff M, Bisgaard A-M, Stoeva R, Dimitrov B: Phenotype and 244k array- CGH characterization of chromosome 13q deletions: An update of the phenotypic map of 13q21.1-qter Am J...
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Báo cáo y học: "Array comparative genomic hybridisation-based identification of two imbalances of chromosome 1p in a 9-year-old girl with a monosomy 1p36 related phenotype and a family history of learning difficulties: a case report" pps
Ngày tải lên :
11/08/2014, 19:21
... purposes) Journal of Medical Case Reports 2008, 2:355 http://www.jmedicalcasereports.com/content/2/1/355 * Figure Array comparative genomic hybridisation scatter plot Array comparative genomic hybridisation ... is evident by the association of a variety of neoplasms in patients with deletions of this chromosome location [8,9] Interstitial duplications of the short arm of chromosome are rare with a varied ... comparative genomic hybridisation scatter plot An array comparative genomic hybridisation scatter plot for chromosome generated by BlueFuse microarray analysis software (BlueGnome) showing the five Bacterial...
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Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"
Ngày tải lên :
25/10/2012, 11:10
... percentage ratio of the hematocrite value of the final loaded cells to that of the initial packed cells, both measured using equal suspension volumes In vitro characterization of pravastatin loaded ... total amount of pravastatin entrapped in 0.1 ml of the final packed erythrocytes Efficiency of entrapment The percentage of the loaded amount of pravastatin to the total amount of that added ... delivery system of primaquine: in vitro characterization J Microencapsul 1992; 9(3):357-364 34 Hamidi M, Tajerzadeh H, Dehpour AR, Rouini MR, Ejtemaee-Mehr S In vitro characterization of human intact...
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