Phân tích hệ glycoprotein huyết thanh bệnh nhân Leukemia

Phân tích hệ glycoprotein huyết thanh bệnh nhân Leukemia Phân tích hệ glycoprotein huyết thanh bệnh nhân Leukemia Phân tích hệ glycoprotein huyết thanh bệnh nhân Leukemia luận văn tốt nghiệp,luận văn thạc sĩ, luận văn cao học, luận văn đại học, luận án tiến sĩ, đồ án tốt nghiệp luận văn tốt nghiệp,luận văn thạc sĩ, luận văn cao học, luận văn đại học, luận án tiến sĩ, đồ án tốt nghiệp

BỘ GIÁO DỤC VÀ ĐÀO TẠO TRƯỜNG ĐẠI HỌC BÁCH KHOA HÀ NỘI NGUYỄN TIẾN DŨNG PHÂN TÍCH HỆ GLYCOPROTEIN HUYẾT THANH BỆNH NHÂN LEUKEMIA LUẬN VĂN THẠC SĨ NGÀNH CÔNG NGHỆ SINH HỌC Hà Nội, 2010 BỘ GIÁO DỤC VÀ ĐÀO TẠO TRƯỜNG ĐẠI HỌC BÁCH KHOA HÀ NỘI NGUYỄN TIẾN DŨNG PHÂN TÍCH HỆ GLYCOPROTEIN HUYẾT THANH BỆNH NHÂN LEUKEMIA LUẬN VĂN THẠC SĨ NGÀNH CÔNG NGHỆ SINH HỌC NGƯỜI HƯỚNG DẪN: TS PHAN VĂN ĐẠT Hà Nội, 2010 LỜI CẢM ƠN Trước hết, xin bày tỏ lòng biết ơn sâu sắc tới GS TS Phan Văn ChiTrưởng phịng Hố Sinh Protein, Viện Cơng nghệ Sinh học, tận tình hướng dẫn truyền thụ cho tơi kiến thức lòng say mê khoa học suốt q trình học tập thực khố luận Tơi xin gửi lời cảm ơn tới PGS.TS Nguyễn Bích Nhi góp ý q báu q trình hồn thành luận văn Tơi xin chân thành cảm ơn anh chị, bạn đồng nghiệp phịng Hố Sinh - Protein, Viện Công Nghệ Sinh Học thầy, cô thuộc Viện Công nghệ Sinh học Công nghệ Thực phẩm, Đại học Bách Khoa Hà Nội, tận tình dạy bảo, giúp đỡ tạo điều kiện thuận lợi để tơi hồn thành khố luận Cuối cùng, tơi xin cảm ơn gia đình, bạn bè người thân - người ln động viên, khích lệ chỗ dựa vững cho suốt trình học tập nghiên cứu Hà Nội, tháng 11 năm 2010 Học viên Nguyễn Tiến Dũng i MỤC LỤC LỜI CẢM ƠN i MỤC LỤC ii CÁC THUẬT NGỮ VIẾT TẮT v DANH MỤC BẢNG SỬ DỤNG TRONG LUẬN VĂN viii DANH MỤC HÌNH SỬ DỤNG TRONG LUẬN VĂN ix MỞ ĐẦU Chương TỔNG QUAN TÀI LIỆU 1.1 Tổng quan bệnh Leukemia 1.1.1 Giới thiệu chung bệnh Leukemia 1.1.2 Nguyên nhân gây bệnh Leukemia 1.1.3 Phân loại Leukemia 1.1.3.1 Leukemia cấp 1.1.3.2 Leukemia kinh 1.1.4 Triệu chứng bệnh Leukemia 1.1.5 Chẩn đoán bệnh Leukemia 1.2 Proteomics – Khoa học nghiên cứu hệ protein 10 1.2.1 Proteomics gì? 10 1.2.2 Proteome huyết 11 1.2.2.1 Đặc điểm, thành phần protein huyết 11 1.2.1.2 Chức protein huyết 11 1.2.1.3 Vì phải nghiên cứu proteome huyết thanh? 12 1.2.2 Quá trình glycosyl hóa 13 1.2.2.1 Đặc điểm trình glycosyl hóa 13 1.2.2.2 Glycoprotein với trình bệnh lý 14 1.2.3 Các công cụ kỹ thuật Proteomics 14 1.2.3.1 Các kỹ thuật phân tách protein 14 1.2.3.2 Khối phổ - Trung tâm proteomics 18 1.2.3.3 Các phần mềm tin sinh học 19 1.2.3.4 Các sở liệu trình tự gen protein 20 1.3 Các nghiên cứu proteomics bệnh ung thư Leukemia 20 1.3.1 Nghiên cứu proteomics bệnh ung thư 20 1.3.2 Nghiên cứu proteomics bệnh Leukemia 21 ii Chương VẬT LIỆU VÀ PHƯƠNG PHÁP NGHIÊN CỨU 24 2.1 Vật liệu 24 2.1.1 Nguyên liệu 24 2.1.2 Hóa chất 24 2.1.3 Trang thiết bị 24 2.2 Các phương pháp nghiên cứu 25 2.2.1 Thu nhận glycoprotein sắc ký lực conA 25 2.2.2 Định lượng protein phương pháp Bradford 26 2.2.3 Làm hỗn hợp glycoprotein phương pháp tủa với acetone 26 2.2.4 Phân tách protein kỹ thuật điện di biến tính gel polyacrylamide 26 2.2.5 Phân tách protein kỹ thuật điện di hai chiều (2DE) 27 2.2.6 Thủy phân protein enzym trypsin 28 2.2.6.1 Thủy phân protein gel 28 2.2.6.2 Thủy phân protein dung dịch – Phân tích tổng thể 29 2.2.7 Phân tách peptide sắc ký lỏng nano đa chiều 29 2.2.8 Ghi phổ NanoLC-ESI- MS/MS 31 2.2.9 Nhận diện protein phần mềm Mascot v1.8 32 2.2.10 Tìm kiếm thơng tin protein 33 Chương KẾT QUẢ VÀ THẢO LUẬN 34 3.1 Lựa chọn mẫu nghiên cứu 34 3.2 Xây dựng phương pháp phân tích hệ glycoprotein huyết bệnh Leukemia 35 3.3 Thu nhận glycoprotein huyết sắc ký lực ConA 36 3.4 Làm mẫu phương pháp tủa acetone 38 3.5 Phân tích mức độ biểu 39 3.5.1 Định lượng protein phương pháp Bradford 39 3.5.2 Phân tách glycoprotein điện di 2-DE 40 3.5.3 Nhận diện protein có mức độ biểu thay đổi hệ 1DNanoLCMS/MS 42 3.5.4 Phân tích thay đổi mức độ biểu phần mềm PDQuest v7.1 44 3.6 Phân tích tổng thể proteome huyết bệnh nhân NHL 47 3.6.1 Ghi phổ 2DnanoLC-MS/MS 47 3.6.2 Nhận diện hệ glycoprotein huyết bệnh nhân Leukemia 48 3.6.3 Tìm kiếm thơng tin liên quan đến glycoprotein nhận diện 49 3.6.4 Nhận diện protein có liên quan đến bệnh ung thư Leukemia 50 3.7 Thảo luận 53 3.7.1 Phương pháp xử lý mẫu 53 iii 3.7.2 Phân tích mức độ biểu điện di 2-DE 54 3.7.3 Phân tích hệ glycoprotein huyết kỹ thuật 2DnanoLC-MS/MS 56 KẾT LUẬN VÀ KIẾN NGHỊ 58 TÀI LIỆU THAM KHẢO 60 PHỤ LỤC Error! Bookmark not defined iv CÁC THUẬT NGỮ VIẾT TẮT 1-DE One-dimensional electrophoresis Điện di chiều 2-DE Two-dimensional electrophoresis Điện di hai chiều 2DnanoLC Two-dimensional nano liquid chromatography Sắc kỷ lỏng nano hai chiều ACN Acetonitrile Acetonitrile ALL Acute lymphocytic leukemia Leukemia cấp dòng lympho AML Acute myeloid leukemia Leukemia cấp dòng tủy amu Atomic mass unit Đơn vị khối lượng nguyên tử APS Ammonium persulfate Ammonium persulfate CHAPS 3-[(3Một chất tẩy rửa lưỡng tính để hịa Cholamidopropyl)dimethylammonio] tan protein CID Collision-induced dissociation Phân mảnh va đập CLL Chronic lymphocytic leukemia Leukemia kinh dòng lympho CML Chronic myeloid leukemia Leukemia kinh dòng tủy Da Dalton Dalton DTT Dithiothreitol Dithiothreitol EBI European Bioinformatics Institute Viện tin sinh học Châu Âu ESI Electrospray ionization Ion hóa phương pháp phun điện FA Formic acid Axít formic HL Hodgkin lymphoma Ung thư lympho dạng Hodgkin v HPLC High performent liquid chromatography Sắc ký lỏng hiệu cao HUPO Human Proteome Organization Tổ chức hệ protein người IAA Iodoacetamide Iodoacetamide IEF Isoelectric focusing Điện di đẳng điện IPG immobilized pH gradient Gradien pH cố định kDa Kilo Dalton Kilo Dalton kV Kilo Volt Kilo Volt m/z Mass to charge ratio Tỷ lệ khối lượng/điện tích MALDI Matrix-asststed laser deorption ionization Ion hóa tác động laze với hỗ trợ chất MS Mass spectrometry Khối phổ MS/MS Tandem mass spectrometry Khối phổ liên tục NanoLC Nano liquid chromatography Sắc ký lỏng nano NCBI National Center for Biotechnology Information Trung tâm quốc gia thông tin Công nghệ sinh học NHL Non-Hodgkin lymphoma Ung thu lympho dạng non-Hodgkin PDB Protein data bank Ngân hàng liệu protein pI Isoelectric point Điểm đẳng điện RP Reversed phase Ngược pha SCX Strong cation exchange chromatography Sắc ký trao đổi cation mạnh SDSPAGE Sodium dodecyl sulphate polyacrylamide gel electrophoresis Điện di biến tính gel polyacrylamide TEMED 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proteins, Genet Eng News, (18), pp 14 68 97 Ye, B., Cramer, D.W., Skates, S.J (2003), Haptoglobin-a subnit as potential serum biomarker in ovarian cancer: identification and characterisation using proteomic profiling and mass spectrometry, Clinical Cancer Research, (9), pp 2904-2911 98 Zhu, H., Bilgin, M., Snyder, M (2003), Proteomics, Annu Rev Biochem, (72), pp.783-812 99 Ziping, Y., William, S.H (2004), Approach the comprehensive analysis of glycoprotein isolate from human serum using a multi-lectin affinity column, Journal of Chromatography A, (1053), pp 79-88 69 Bảng phụ lục Danh sách glycoprotein huyết bệnh nhân CML nhận diện STT Mã số GI Mã Uniprot gi|12004292 Q9H2U9 A disintegrin and metalloproteinase Membrane gi|21265058 Q8TE58 gi|27463365 Q9P2N4 A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type motif, 15 preproprotein A disintegrin-like and metalloprotease with thrombospondin type motifs 9B gi|51558724 P58397 gi|9280405 Vị trí Chức Biến đổi sau dịch mã Extracellular matrix Metalloendopeptidase activity, zinc ion binding Hydrolase, Metalloprotease, Protease Extracellular matrix Hydrolase, Metalloprotease, Protease ADAM metallopeptidase with thrombospondin type motif, 12 preproprotein Extracellular matrix Hydrolase, Metalloprotease, Protease Q9NR99 Adlican Unknown Unknown gi|4502027 P02768 Albumin preproprotein Protein complex, platelet alpha granule lumen, extracellular space Toxin binding, pyridoxal phosphate binding, fatty acid binding, drug binding, copper ion binding gi|2521983 P02765 Alpha-2-HS-glycoprotein Extracellular space Kinase inhibitor activity, protein binding gi|55743098 P12111 Alpha type VI collagen isoform precursor Collagen type VI Protein binding, serine-type endopeptidase inhibitor activity gi|179730 P12107 Alpha-1 (type XI) collagen precursor Collagen type XI 10 gi|180758 Q07092 Alpha-1 type XVI collagen Extracellular matrix, Secreted 11 gi|225768 P01009 Alpha-1-antitrypsin 12 gi|2281752 O00555 Alpha1A-voltage-dependent calcium channel Proteinaceous extracellular matrix, platelet alpha granule lumen, extracellular space Membrane Extracellular matrix binding, extracellular matrix structural constituent, protein binding, bridging Integrin binding, structural molecule activity Serine-type endopeptidase inhibitor activity, protease binding Disulfide bond, Glycoprotein Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Phosphoprotein, Zymogen Disulfide bond, Glycoprotein Cleavage on pair of basic residues, Disulfide bond, Glycation, Glycoprotein, Phosphoprotein Disulfide bond Glycoprotein Phosphoprotein Disulfide bond, Glycoprotein, Hydroxylation, Pyrrolidone carboxylic acid Glycoprotein, Hydroxylation 13 gi|29336810 Q8TE59 A disintegrin and metalloproteinase with thrombospondin motifs 19 Tên Protein Proteinaceous extracellular matrix Calcium channel, Ionic channel, Voltage-gated channel Metalloendopeptidase activity, zinc ion binding Bệnh liên quan 4N 9N 14N 8N Disease mutation 25N 2N, 3O Disease mutation 10N, 5O Deafness, Disease mutation, Stickler syndrome 1N Glycoprotein, Hydroxylation Glycoprotein Disulfide bond, Glycoprotein, Phosphoprotein Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Phosphoprotein, Zymogen Số vị trí glycosyl hóa 6N 2N 3N Disease mutation, Neurodegeneration 1N 5N 14 gi|46577680 P04217 Alpha-1B-glycoprotein Extracellular region Unknown 15 gi|112911 P01023 Alpha-2-macroglobulin Unknown Protease inhibitor, Serine protease inhibitor 16 gi|48428570 O75443 Alpha-tectorin Cell membrane, Extracellular matrix, Membrane Unknown 17 gi|28780 P04114 Apo-B100 precursor Cytoplasm, Nucleus Chromatin regulator, Receptor 18 gi|458032 Q12797 Aspartyl beta-hydroxylase Dioxygenase, Oxidoreductase 19 gi|157266317 Q13535 20 gi|5734101 O95477 Ataxia telangiectasia and Rad3 related protein ATP cassette binding transporter Endoplasmic reticulum Membrane Chromosomal protein, Nucleus 21 gi|338443 Q01082 Beta-spectrin Cell membrane, Cytoplasm, Cytoskeleton, Membrane 22 gi|2696015 Q15413 Brain ryanodine receptor Integral to membrane 23 gi|20800447 Q4LE39 Breast cancer-associated antigen BRCAA1 Unknown 24 gi|7656967 Q9NYQ6 Cadherin EGF LAG seven-pass G-type receptor Cell membrane, Membrane Developmental protein, G-protein coupled receptor, Receptor, Transducer 25 gi|13325064 Q9HCU4 Cadherin EGF LAG seven-pass G-type receptor Cell membrane, Membrane 26 gi|22095552 Q9NYQ7 Cadherin EGF LAG seven-pass G-type receptor Cell membrane, Membrane 27 gi|23307795 Q8IUL8 28 gi|1620909 P00450 Cartilage intermediate layer protein-like protein CLIP-2 Ceruloplasmin Secreted, extracellular space, extracellular matrix Extracellular space Developmental protein, G-protein coupled receptorm Receptor, Transducer Developmental protein, G-protein coupled receptor, Receptor, Transducer Unknown 29 gi|1846005 Q99715 Collagen type XII alpha-1 Collagen type XII 30 gi|55743096 Q05707 Collagen, type XIV, alpha Collagen type XIV 31 gi|115298678 P01024 Complement component precursor Receptor binding Membrane Kinase, Serine/threonine-protein kinase, Transferase ATP binding, ATPase activity, anion transmembrane transporter activity, apolipoprotein A-I receptor activity, cholesterol binding Actin capping Ryanodine-sensitive calcium-release channel activity, receptor activity, calcium ion binding Unknown Chaperone binding, ferroxidase activity Extracellular matrix structural constituent conferring tensile strength, protein binding Collagen binding, extracellular matrix structural constituent, protein binding, bridging Endopeptidase inhibitor activity, receptor binding Disulfide bond, Glycoprotein Disulfide bond, Glycoprotein, Isopeptide bond, Thioester bond GPI-anchor, Glycoprotein, Lipoprotein Isopeptide bond, Phosphoprotein, Ubl conjugation Glycoprotein, Phosphoprotein Phosphoprotein Disulfide bond, Glycoprotein, Phosphoprotein 4N 8N Disease mutation, Nonsyndromic deafness 29N Disease mutation, Pseudohermaphroditism 29N 2N Dwarfism 5N Atherosclerosis, Disease mutation 26N Acetylation, Glycoprotein, Phosphoprotein Glycoprotein 1O 6N Unknown 5N Disulfide bond Glycoprotein, Hydroxylation, Phosphoprotein Disulfide bond, Glycoprotein, Hydroxylation Disulfide bond, Glycoprotein, Hydroxylation Glycoprotein 20N Disulfide bond Glycoprotein Phosphoprotein Disulfide bond, Glycoprotein, Hydroxylation Disulfide bond, Glycoprotein, Hydroxylation Cleavage on pair of basic residues, Disulfide bond, 6N 17N 15N 3N 3O, 5N 12N Disease mutation 3N 32 gi|178557739 P0C0L5 Complement component 4B preproprotein Extracellular space Endopeptidase inhibitor activity, protein binding 33 gi|306680 P17927 Complement receptor Integral to plasma membrane Complement receptor activity 34 gi|307228 P13671 Completement component C6 Membrane attack complex Protein binding 35 gi|38045888 Q7Z407 gi|13259127 Q13620 Integral to membrane, plasma membrane Nucleus Unknown 36 CUB and Sushi multiple domains isoform Cullin CUL4B 37 gi|16933557 Q96JQ0 Dachsous precursor Membrane Calcium ion binding, protein binding, 38 gi|7671639 P22105 dJ34F7.1.1 (tenascin XB (isoform 1)) Integrin binding, heparin binding 39 gi|20127422 O60469 Down syndrome cell adhesion molecule isoform CHD2-42 precursor 40 gi|19923559 Q7Z589 EMSY protein Proteinaceous extracellular matrix, intracellular, extracellular space Axon, extracellular region, growth cone, integral to plasma membrane, membrane fraction Nucleus 41 gi|1345961 P35556 Fibrillin-2 42 gi|4758284 P54762 Ephrin receptor EphB1 precursor Proteinaceous extracellular matrix Integral to plasma membrane 43 gi|54792100 P21860 ErbB-3 isoform precursor 44 gi|4557565 Q03468 45 gi|58430435 Q5H8C1 Excision repair cross-complementing rodent repair deficiency, complementation group [Homo sapiens Extracellular matrix protein QBRICK 46 gi|38195903 Q6V0I7 Fat-like cadherin FATJ protein Integral to membrane, plasma membrane Calcium ion binding, extracellular matrix structural constituent Kinase, Receptor, Transferase, Tyrosine-protein kinase, ATP binding, ephrin receptor activity, protein binding Kinase, Receptor, Transferase, Tyrosine-protein kinase ATP binding, DNA binding, DNAdependent ATPase activity, chromatin binding, helicase activity Developmental protein, metal ion binding, protein binding, sugar binding Calcium ion binding 47 gi|18676450 Q8WWQ8 FLJ00122 protein Integral to plasma membrane, external side of plasma membrane, cytoplasm Cell membrane, Membrane, Secreted Nucleolus, soluble fraction, transcription elongation factor complex Basement membrane, integral to membrane Ubiquitin protein ligase binding Protein binding Unknown Scavenger receptor activity, protein disulfide oxidoreductase activity, lowdensity lipoprotein receptor activity Glycoprotein, Phosphoprotein, Thioester bond Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Sulfation, Thioester bond Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid Disulfide bond, Glycoprotein, Phosphoprotein Disulfide bond, Glycoprotein Isopeptide bond, Phosphoprotein, Ubl conjugation Glycoprotein Disulfide bond, Glycoprotein, Phosphoprotein Disulfide bond, Glycoprotein, Phosphoprotein Glycoprotein, Phosphoprotein Disulfide bond, Glycoprotein Glycoprotein, Phosphoprotein 4N 20N 2N, 6O 41N Disease mutation, Mental retardation 14N Ehlers-Danlos syndrome 7N 18N 1O Disease mutation 12N 3N Glycoprotein Transcription elongation factor complex 7N 10N Cockayne syndrome, Deafness, Disease mutation 5N Disulfide bond, Glycoprotein 5N Disulfide bond, Glycoprotein, Phosphoprotein Disulfide bond, Glycoprotein, Phosphoprotein, Proteoglycan 38N 28N 48 gi|62088170 P47869 Gamma-aminobutyric acid A receptor, alpha precursor variant Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse ER-Golgi intermediate compartment, Golgi membrane, Golgi stack, integral to membrane Coated pit, integral to membrane, lysosome Chloride channel, Ionic channel Disulfide bond, Glycoprotein 2N 49 gi|405715 Q14789 Giantin Protein binding Disulfide bond, Phosphoprotein 12N 50 gi|1809240 P98164 Gp330 precursor SH3 domain binding, calcium ion binding, receptor activity 40N GPR158-like receptor Cell membrane, Membrane P00738 Haptoglobin Extracellular region Q6DFV5 Helicase with zinc finger domain Nucleus Disulfide bond Glycoprotein Phosphoprotein 4N gi|54792138 54 gi|14575679 Q96RW7 Hemicentin Basement membrane G-protein coupled receptor, Receptor, Transducer Hemoglobin binding, serine-type endopeptidase activity ATP binding, helicase activity, nucleic acid binding, zinc ion binding Calcium ion binding Disulfide bond, Glycoprotein, Phosphoprotein Glycoprotein 51 gi|46397615 Q6PRD1 52 gi|1212947 53 55 gi|11602963 P98160 Heparan sulfate proteoglycan perlecan Basement membrane, Extracellular matrix, Secreted Protein C-terminus binding 56 gi|4504489 P04196 Histidine-rich glycoprotein precursor 57 gi|1657698 O75330 Hyaluronan receptor Extracellular region, platelet alpha granule lumen Cytoplasm Cysteine-type endopeptidase inhibitor activity, heparin binding Hyaluronic acid binding 58 gi|184749 P01876 Unknown Antigen binding , protein binding 59 gi|4467842 P01871 Immunoglobulin alpha-1 heavy chain constant region Immunoglobulin M heavy chain Hemoglobin complex 60 gi|38490688 Q6WRI0 Immunoglobulin superfamily, member 10 Extracellular region Heme binding, oxygen binding,oxygen transporter activity Developmental protein 61 gi|55664251 P53708 Integrin, alpha Membrane 62 gi|39652254 Q06033 Inter-alpha-trypsin inhibitor heavy chain H3 Extracellular region 63 gi|127506 P04220 Ig mu heavy chain disease protein Membrane fraction 64 gi|2833252 Q14571 Inositol 1,4,5-trisphosphate receptor type Integral to membrane, plasma membrane enriched fraction 65 gi|40788245 O43157 KIAA0407 Membrane Calcium ion transmembrane transporter activity, inositol 1,4,5trisphosphate-sensitive calciumrelease channel activity Receptor 66 gi|40788312 Q7L0X0 KIAA0644 protein Integral to membrane Protein binding 67 gi|20521694 Q9P2S2 KIAA0921 protein Integral to membrane Metal ion binding, protein binding Receptor activity, protein binding, calcium ion binding Serine-type endopeptidase inhibitor activity Antigen binding Disulfide bond, Glycoprotein Disulfide bond, Glycoprotein, Heparan sulfate, Proteoglycan Disulfide bond, Glycoprotein Glycoprotein, Phosphoprotein, Proteoglycan Disulfide bond, Glycoprotein Acetylation, Phosphoprotein Disulfide bond, Glycoprotein, Phosphoprotein Glycoprotein 2N 5N Disease mutation 34N Disease mutation 8N, 6O Disease mutation, Thrombophilia 4N 4N 1N, 5O 5N 8N 16N Glycoprotein, Proteoglycan Glycoprotein, Phosphoprotein Acetylation, Phosphoprotein 2N Glycoprotein, Phosphoprotein Glycoprotein 6N Disulfide bond, Glycoprotein 4N 1N 9N 3N 68 gi|7243225 Q5JUK3 KIAA1422 protein Integral to membrane, plasma membrane Endoplasmic reticulum, Membrane Binding, calcium-activated potassium channel activity, catalytic activity Unknown Lactoferrin Secreted Hydrolase, Protease Serine protease P25391 Laminin A chain Extracellular matrix structural constituent, receptor binding gi|28559088 P24043 Laminin alpha subunit isoform a precursor Extracellular space Laminin-1 complex Laminin-3 complex Laminin-1 complex Glycoprotein, Phosphoprotein Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation Disulfide bond, Glycoprotein, Zymogen Disulfide bond, Glycoprotein 69 gi|33620775 Q86UP2 Kinectin isoform a 70 gi|187122 P02788 71 gi|34226 72 Receptor binding, structural molecule activity Disulfide bond, Glycoprotein 73 gi|62087424 Q59H37 Laminin alpha subunit precursor variant Laminin-1 complex Receptor binding Unknown 74 gi|38045910 Q16787 Laminin alpha subunit isoform Unknown gi|167614504 P07942 Laminin, beta precursor Disulfide bond, Glycoprotein Disulfide bond, Glycoprotein Epidermolysis bullosa 75 76 gi|2429079 Q13751 Laminin-5 beta3 chain Basement membrane, Extracellular matrix Extracellular space Laminin-1 complex Laminin-10 complex Laminin-2 complex Basement membrane Disease mutation, Epidermolysis bullosa 77 gi|19923729 Q8TDW0 gi1139595 P48357 Endoplasmic reticulum Membrane Membrane Protein binding 78 Leucine rich repeat containing family, member C Leptin receptor 79 gi|22036115 Q86UK5 Limbin Integral to membrane Plays a critical role in bone formation and skeletal development Disulfide bond, Glycoprotein Glycoprotein, Phosphoprotein Disulfide bond Glycoprotein Phosphoprotein Glycoprotein 80 gi|312148 Q86VB7 M130 antigen extracellular variant Extracellular region, integral to plasma membrane Protein binding, scavenger receptor activity 81 gi|9211112 Q8IZY2 Macrophage ABC transporter ATP binding, ATPase activity 82 gi|58801252 O75094 MEGF5 83 gi|2498589 Q13330 Metastasis-associated protein MTA1 Cell membrane, Endosome, Golgi apparatus, Membrane Mitochondrion, extracellular space Cytoplasm 84 gi|3449306 O75096 MEGF7 Membrane 85 gi|4506775 Q14703 Membrane-bound transcription factor site-1 protease preproprotein Golgi membrane, endoplasmic reticulum membrane, nucleus Serine-type endopeptidase activity 86 gi|464228 Q04656 Menkes disease protein Cell membrane, Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane Hydrolase Extracellular matrix structural constituent, protein binding Structural molecule activity Receptor Calcium ion binding, Roundabout binding Sequence-specific DNA binding, transcription factor activity, zinc ion binding Receptor 2N 8N Disease mutation 3N 34N Congenital muscular dystrophy, Disease mutation 28N 13N 5N 11N 3N 5N Disease mutation, Obesity Disease mutation, Dwarfism, Ectodermal dysplasia 1N Disulfide bond, Glycoprotein, Phosphoprotein Disulfide bond, Glycoprotein Disulfide bond, Glycoprotein Phosphoprotein 4N Disulfide bond, Glycoprotein, Phosphoprotein Autocatalytic cleavage, Glycoprotein, Phosphoprotein, Zymogen Glycoprotein, Phosphoprotein 7N 1N 1O 5N 5N Disease mutation 2N 87 gi|47519639 P27816 Microtubule-associated protein isoform Cell membrane, Membrane G-protein coupled receptor, Receptor, Transducer 88 gi|31144 P11055 Myosin heavy chain (1167 AA) Cytoplasm,Cytoskeleton, Nucleus 89 gi|1613788 Q13402 Myosin VIIa Cytosol, lysosomal membrane, myosin complex 90 gi|38202211 Q3T906 Golgi apparatus membrane 91 gi|55959399 O94856 N-acetylglucosamine-1-phosphate transferase Neurofascin homolog (chicken) Actin binding, phosphatidylinositol binding, spectrin binding, structural constituent of cytoskeleton ATP binding, actin binding , calmodulin binding, microfilament motor activity Transferase Cell membrane, Membrane Protein binding 92 gi|3982598 Q9UPA5 Neuronal double zinc finger protein Zinc ion binding 93 gi|1899202 Q13224 N-methyl-D-aspartate receptor 2B subunit precursor 94 gi|179106 Q13813 Nonerythroid alpha-spectrin Cell junction, cytoplasm, cytoskeleton, nucleus, synapse, synaptosome N-methyl-D-aspartate selective glutamate receptor complex, cell junction Spectrin, membrane fraction, cytosol 95 gi|134244285 Q9UM47 Notch homolog integral to membrane, nucleus, plasma membrane 96 gi|505665 Q12866 Novel cellular proto-oncogene Integral to plasma membrane, soluble fraction 97 gi|940539 P48552 Nuclear factor RIP140 Intermediate filament, Keratin 98 gi|307180 P08183 P-glycoprotein Cell membrane, Membrane Calcium:sodium antiporter activity, catalytic activity, neutral amino acid transmembrane transporter activity,protein binding 99 gi|1888354 Q9UIQ6 Placental leucine aminopeptidase 100 gi|35825 P20742 Pregnancy zone protein Cell membrane, Membrane, Secreted Extracellular space Aminopeptidase, Hydrolase, Metalloprotease, Protease Protein binding, serine-type endopeptidase inhibitor activity 101 gi|178775 P02647 Proapolipoprotein Endocytic vesicle, endoplasmic reticulum lumen, plasma membrane 102 gi|1479976 Q15262 Protein tyrosine phosphatase Cell junction, Cell membrane, Membrane Apolipoprotein A-I receptor binding, beta-amyloid binding, cholesterol binding, cholesterol transporter activity Transmembrane receptor protein tyrosine phosphatase activity, protein kinase binding, gamma-catenin bindinG N-methyl-D-aspartate selective glutamate receptor activity, glycine binding, zinc ion binding Structural constituent of cytoskeleton, calmodulin binding, calcium ion binding, actin binding Calcium ion binding, protein binding, receptor activity ATP binding, protein binding, transmembrane receptor protein tyrosine kinase activity Structural molecule activity Disulfide bond, Glycoprotein, Lipoprotein, Palmitate Glycoprotein, Phosphoprotein Phosphoprotein Disulfide bond, Glycoprotein Disulfide bond, Glycoprotein, Phosphoprotein Glycoprotein, Lipoprotein, Myristate, Phosphoprotein Disulfide bond, Glycoprotein, Phosphoprotein Acetylation, Phosphoprotein Disulfide bond, Glycoprotein, Phosphoprotein Disulfide bond, Glycoprotein, Phosphoprotein Phosphoprotein Acetylation, Disulfide bond, Glycoprotein,Isopeptide bond, Phosphoprotein, Ubl conjugation Glycoprotein, Phosphoprotein Disulfide bond, Glycoprotein, Thioester bond Glycation, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein Glycoprotein Unknown Elliptocytosis, Hereditary hemolytic anemia Deafness, Disease mutation, Nonsyndromic deafne Disease mutation, Mucolipidosis Unknown 6N 11N 8N 5O 7N 6N Disease mutation 3N Disease mutation, Proto-oncogene 14N 6N 3N 17N 11N 1N 12N 103 gi|94538350 O14917 Protocadherin 17 precursor Cell membrane, Membrane Calcium ion binding Glycoprotein 7N 104 gi|1304314 P54886 Pyrroline 5-carboxylate synthetase Cytoplasm, Nucleus Acetylation 2N 105 gi|622985 Q06418 Receptor tyrosine kinase Cell membrane, Membrane Glycoprotein 7N 106 gi|2498589 Q13330 Metastasis-associated protein MTA1 Cytoplasm Phosphoprotein 5N 107 gi|26006802 Q8NCM2 Membrane gi|1708865 Q07954 Glycoprotein, Isopeptide bond, Ubl conjugation Disulfide bond, Glycoprotein, Phosphoprotein 1N 108 Potassium voltage-gated channel subfamily H member Prolow-density lipoprotein receptor-related protein 109 gi|126469 P23467 Receptor-type tyrosine-protein phosphatase beta Integral to plasma membrane 110 gi|134594 P12757 Ski-like protein (Ski-related oncogene) Extracellular matrix, Secreted Importin-alpha export receptor activity Kinase, Receptor, Transferase, Tyrosine-protein kinase Sequence-specific DNA binding, transcription factor activity, zinc ion binding Ionic channel, Potassium channel, Voltage-gated channel Apolipoprotein binding, lipid transporter activity, lipoprotein binding, lipoprotein receptor binding, receptor activity Protein binding, transmembrane receptor protein tyrosine phosphatase activity Calcium ion binding, hyaluronic acid binding , sugar binding 111 gi|1706534 P50443 Diastrophic dysplasia protein Membrane 112 gi|27436938 P78509 Reelin isoform a Extracellular matrix, Secreted 113 gi|337722 P21817 Ryanodine receptor Terminal cisterna, smooth endoplasmic reticulum 114 gi|1526978 Q92736 Ryanodine receptor 115 gi|24234741 Q8NFY4 Semaphorin 6D isoform precursor Reticulum membrane, plasma membrane enriched fraction, plasma membrane Plasma membrane, integral to membrane, cytoplasm 116 gi|25014054 Q9NY46 Membrane 117 gi|115583667 Q9UI33 118 gi|4507157 Q92673 Sodium channel protein type subunit alpha Sodium channel, voltage-gated, type XI, alpha Sortilin-related receptor containing LDLR class A repeats preproprotein 119 gi|62089108 Q01826 120 gi|55662684 Q4LDE5 121 gi|37227 P24821 Special AT-rich sequence binding protein variant Sushi, von Willebrand factor type A, EGF and pentraxin domain containing Tenascin Golgi stack, Golgi-associated vesicle Membrane, extracellular region, cytoplasm Extracellular matrix, Secreted Coated pit, integral to plasma membrane, membrane fraction, nucleus Membrane LDL, Membrane Secondary active sulfate transmembrane transporter activity Developmental protein, Hydrolase, Protease, Serine protease Ryanodine-sensitive calcium-release channel activity, receptor activity, calcium ion binding, calmodulin binding Suramin binding, ryanodine-sensitive calcium-release channel activity, receptor activity Receptor activity Ionic channel, Sodium channel, Voltage-gated channel Ionic channel, Sodium channel, Voltage-gated channel Receptor Hydrolase Protein binding, calcium ion binding Receptor binding 52N Glycoprotein, Phosphoprotein 26N Disulfide bond, Glycoprotein, Phosphoprotein, Proteoglycan Glycoprotein, Phosphoprotein Disulfide bond, Glycoprotein Glycoprotein, Phosphoprotein, Snitrosylation Cataract Glycoprotein, Phosphoprotein Disulfide bond, Glycoprotein, Phosphoprotein Glycoprotein, Ubl conjugation Glycoprotein, Phosphoprotein Cleavage on pair of basic residues, Disulfide bond, Glycoprotein Unknown Disulfide bond, Glycoprotein Disulfide bond, Glycoprotein, Phosphoprotein Unknown Disease mutation, Dwarfism Lissencephaly 3N 19N Disease mutation 6N Cardiomyopathy, Disease mutation 10N 5N 13N 8N 27N Cataract, Disease mutation 8N 5N 22N 122 gi|6165845 Q9UKZ4 Tenascin-M1 Integral to membrane, extracellular region Heparin binding 123 gi|21655209 P08F94 TIG multiple domains-1 Cytoplasm, Cytoskeleton Muscle protein 124 gi|553788 P02787 Ttransferrin Unknown Unknown Disulfide bond, Glycoprotein, Phosphoprotein Acetylation, Isopeptide bond, Ubl conjugation Unknown 13N 125 gi|8922720 Q9NV96 Transmembrane protein 30A isoform Membrane Unknown Glycoprotein 3N 126 gi|574806 P53420 Type IV collagen alpha chain gi|4502955 P29400 Type IV collagen alpha isoform precursor Protein binding, signal transducer activity, structural molecule activity Protein binding, extracellular matrix structural constituent 128 gi|9910280 Q9NYU2 Endoplasmic reticulum Glycosyltransferase, Transferase 129 gi|4406698 Q6P9A2 UDP-glucose ceramide glucosyltransferaselike isoform Unknown Golgi apparatus, Membrane Glycosyltransferase, Transferase 130 gi|1335098 P02790 Unnamed protein product Extracellular space 131 gi|37947 P04275 Unnamed protein product Cytoplasm Heme transporter activity, metal ion binding Argininosuccinate lyase activity Acetylation, Phosphoprotein Disulfide bond, Glycoprotein, Hydroxylation Glycoprotein, Phosphoprotein Disulfide bond, Glycoprotein Disulfide bond, Glycoprotein Acetylation 20N 127 Coated pit, Cytoplasmic vesicle, Membrane Collagen type IV 132 gi|28334 P12814 Unnamed protein product Cytoplasm, Cytoskeleton Binding, structural molecule activity 133 gi|16552183 Q6UWL2 Unnamed protein product Membrane Calcium ion binding 134 gi|34532092 Q6ZUB1 Unnamed protein product Membrane 135 gi|34531028 Q6ZV29 Unnamed protein product 136 gi|47077384 Q92954 Unnamed protein product 137 gi|34812 P46939 Utrophin (dystrophin related protein) 138 gi|1304105 Q01668 Voltage-dependent calcium channel alpha1 subunit, CACN4 139 gi|495868 Q15878 140 gi|28269693 141 gi|38026 Cardiomyopathy, Disease mutation 64N 2N, 1O Alport syndrome, Disease mutation 1N 2N 3N 5N, 1O Disease mutation 26N 7N Unknown Acetylation, Phosphoprotein Disulfide bond, Glycoprotein Glycoprotein Endoplasmic reticulum, Lysosome, Membrane, Microsome, Mitochondrion, Nucleus Extracellular region Hydrolase activity Glycoprotein 4N Polysaccharide binding, protein binding, scavenger receptor activity 2N Postsynaptic membrane, membrane fraction, cell junction Membrane Zinc ion binding, calcium ion binding Disulfide bond, Glycoprotein, Phosphoprotein, Proteoglycan Phosphoprotein 3N Voltage-dependent calcium channel alpha1E-1 Membrane Voltage-gated calcium channel activity Q86Y38 Xylosyltransferase I P25311 Zinc-α2 –glycoprotein Endoplasmic reticulum membrane, Golgi membrane Extracellular region, MHC class I protein complex Protein xylosyltransferase activity, acetylglucosaminyltransferase activity Fatty acid binding, protein transmembrane transporter activity, ribonuclease activity Disulfide bond, Glycoprotein, Phosphoprotein Disulfide bond, Glycoprotein, Phosphoprotein Disulfide bond, Glycoprotein Disulfide bond Glycoprotein Pyrrolidone carboxylic acid Calcium channel, Ionic channel, Voltage-gated channel 4N 6N 2N 3N 4N ... tài: ? ?Phân tích hệ glycoprotein huyết bệnh nhân Leukemia? ?? với nội dung sau: Xây dựng phương pháp nghiên cứu hệ glycoprotein huyết người So sánh mức độ biểu glycoprotein huyết bệnh nhân Leukemia. .. tài: ? ?Phân tích hệ glycoprotein huyết bệnh nhân Leukemia? ?? với nội dung sau: Xây dựng phương pháp nghiên cứu hệ glycoprotein huyết người So sánh mức độ biểu glycoprotein huyết bệnh nhân Leukemia. .. sớm bệnh Leukemia Phân tích hệ glycoprotein huyết bệnh nhân Leukemia phát glycoprotein có khả liên quan đến bệnh Chương TỔNG QUAN TÀI LIỆU 1.1 Tổng quan bệnh Leukemia 1.1.1 Giới thiệu chung bệnh