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Nghiên cứu được thực hiện trên 876 mẫu tôm giống thu tại các tỉnh Ninh Thuận, Bình Thuận, Vũng Tàu và ĐBSCL và 141 mẫu nuôi theo mô hình QC/QCCT được thu tại các tỉnh Bạc Liêu, Sóc Trăng, Cà Mau và Kiên Giang từ tháng 1 đến tháng 11 năm 2017.
i Trang: 184 Tài liệu tiếng Anh Chayaburakul K., Gary Nash, Phusit Pratanpipat, Siriporn Sriurairatana, Boonsirm Withyachumnarnkul, 2004 Multiple pathogens found in growth-retarded black tiger shrimp Penaeus monodon cultivated in Thailand 60: 89–96 Flegel, T.W (2012) Historic emergence, impact and current status of shrimp pathogens in Asia Journal of Invertebr Pathol 110: 166-173 Gao, H., Kong, J., Li, Z., Xiao, G., Meng, X, 2011 Quantitativeanalysis of temperature, salinity and pH on WSSV pro -liferation in Chinese shrimp Fenneropenaeus chinensisby real-time PCR Aquaculture312: 26-31 Han, J.E., Mohney, L.L., Tang, K.F.J., Pantoja, C.R., Lightner, D.V, 2015 Plasmid mediated tetracycline resistance of Vibrio parahaemolyticus associated with acute TẠP CHÍ NGHỀ CÁ SƠNG CỬU LONG - SỐ 10 - THÁNG 12/2017 55 VIỆN NGHIÊN CỨU NUÔI TRỒNG THỦY SẢN II hepatopancreatic necrosis disease (AHPND) in shrimps Aquaculture Reports (2): 17–21 Huang, C., Zhang, L., Zhang, J., Xiao, L., Wu, Q., Chen, D., Li, J.K, 2001 Purification and characterization of White Spot syndrome virus (WSSV) produced in an alternate host: crayfish, Cambarus clarkii Virus Res.76(2):115-25 Kesavan K, Mani R, Toshiaki I, Sudhakaran R, 2016 Quick report on prevalence of shrimp microsporidian parasite Enterocytozoon hepatopenaei in India Aquaculture Research Kiatpathomchai W, Boonsaeng V, Tassanakajon A, Wongteerasupaya C, Jitrapakdee S, Panyim S, 2001.A non-stop, single-tube, semi-nested PCR technique for grading the severity of white spot syndrome virus infections in Penaeus monodon Dis Aquat Organ 2001 Dec 5;47(3):235-9 Lightner, D.V., Redman, R.M., Pantoja, C.R., Noble, B.L., Loc Tran, 2012 Early Mortality Syndrome Global aquaculture advocate 2/2012, p40 Panakorn, S., 2012 Opinion article: more on early mortality syndrome in shrimp Aqua Culture Asia Pacific, (1): 8-10 Phuoc, L.H., Corteel, M., Nauwynck, H.J., Pensaert, M.B., Alday-Sanz, V., Van den Broeck, W., Sorgeloos, P., Bossier, P., 2008 Increased susceptibility of white spot syndrome virus infected Litopenaeus vannamei to Vibrio campbellii J Environ Microbiol 10 (10), 2718–2727 Phuoc, L.H., Corteel, M., Thanh, NC., Nauwynck, H.J., Pensaert, M.B., Alday-Sanz, V., Van den Broeck, W., Sorgeloos, P., Bossier, P., 2009 Effect of dose and challenge routes of Vibrio spp on co-infection with white spot syndrome virus in Penaeus vannamei Aquaculture290: 61–68 Prachumwat, A.A., Thitamadee, S., Sriurairatana, S., Chuchird, N., Limsuwan, C Jantratit, W., Chaiyapechara, S., Flegel, T.W., 2012 Shotgun sequencing of bacteria from AHPNS, a new shrimp disease threat for Thailand Poster, National Institute for Aquaculture Biotechnology, Mahidol University, Bangkok, Thailand (Poster available for free download at www.enaca.org) 56 Rahman, M.M., Corteel, M., Dantas-Lima, J.J., Wille, M., Alday-Sanz, V., Pensaert, M.B., Sorgeloos, P., Nauwynck, H.J., 2007a Impact of daily fluctuations of optimum (27°C) and high water temperature (33°C) on Penaeus vannamei juveniles infected with white spot syndrome virus (WSSV) Aquaculture 269: 107–113 Rahman, M.M., Corteel, M., Wille, M., AldaySanz, V., Pensaert, M.B., Sorgeloos, P., Nauwynck, H.J., 2007b The effect of raising water temperature to 33°C in Penaeus vannamei juveniles at different stages of infection with white spot syndrome virus (WSSV) Aquaculture272: 240–245 Rajendran, K.V., Vijayan, K.K., Santiago, T.C., 1999 Experimental host range and histopathology of white spot syndrome virus (WSSV) in shrimps, prawns, crabs and lobsters from India with a note on the carrier/reservoir hosts J Fish Dis22: 183-191 Tang, K.F.J., Pantoja, C.R., Redman, R.M., Han, J.E., Tran, L.H., Lightner, D.V., 2015 Development of in situ hybridization and PCR assays for the detection of Enterocytozoon hepatopenaei (EHP), a microsporidian parasite infecting penaeid shrimp J Invertebr Pathol.130: 37–41 Tendencia, E.A., Verreth, J.A.J., 2011 Temperature fluctuation, low salinity, watermicroflora: risk factors for WSSV outbreaks in Penaeus monodon Isr J Aquacult-Bamid 63: 1−7 Thitamadee, S., Prachumwat, A., Srisala, J., Jaroenlak, P., Salachan, P.V., Sritunyalucksana, K., Flegel, T.W., Itsathitphaisarn, O., 2016 Review of current disease threats for cultivated penaeid shrimp in Asia Aquaculture 452, 6987 Tourtip, S., Wongtripop, S., Stentiford, G.D., Bateman, K.S., Sriurairatana, S., Chavadej, J., Sritunyalucksana, K., Withyachumnarnkul, B., 2009 Enterocytozoon hepatopenaei sp Nov (Microsporida: Enterocytozoonidae), a parasite of the black tiger shrimp Penaeus monodon (Decapoda: Penaeidae): Fine structure and phylogenetic relationships Journal of invertebrate pathology 102(1): 21-29 TẠP CHÍ NGHỀ CÁ SƠNG CỬU LONG - SỐ 10 - THÁNG 12/2017 VIỆN NGHIÊN CỨU NUÔI TRỒNG THỦY SẢN II OCCURRENCE OF WSSV, Vibrio parahaemolyticus CAUSING AHPND AND EHP IN POSTLARVAE AND EXTENSIVE-FARMED SHRIMP IN THE MEKONG DELTA, VIETNAM IN 2017 Nguyen Hong Loc1, Le Hong Phuoc1* ABSTRACT 879 postlarvae samples collected at Ninh Thuan, Binh Thuan, Vung Tau and in the Mekong Delta and 141 extensive-farmed shrimp samples collected at Bac Lieu, Soc Trang, Ca Mau and Kien Giang were tested for several pathogens including WSSV, Vibrio parahaemolyticus and EHP by PCR technique In the dry season, the percentage of postlarvae samples positive with WSSV, Vibrio parahaemolyticus and EHP were 1.17%; 1.67% and 1.4%, respectively; and the percentage of farmed shrimp samples positive with WSSV, Vibrio parahaemolyticus and EHP were 8.08; 6.45% and 4.87%, respectively In the rainy season, the percentage of postlarvae samples positive with WSSV, Vibrio parahaemolyticus and EHP were 0.67%; 3.11% and 4%, respectively; and the percentage of farmed shrimp samples positive with WSSV, Vibrio parahaemolyticus and EHP were 2.53%; 2.53% and 10.73%, respectively In comparison with the percentage of postlarve samples positive with the three pathogenic agents collected in 2015 and 2016, the percentage of postlarve samples positive with V parahaemolyticus in 2017 was higher; the percentage of postlarve samples positive with WSSV in 2017 was lower, and the percentage of postlarve samples positive with EHP in 2017 descreased about three times Keywords: Acute Hepatopancreatic Necrosis Disease; Entorocytozoon hepatopenaei; Polymerase Chain Reaction Người phản biện: TS Đinh Thị Thủy Ngày nhận bài: 02/12/2017 Ngày thông qua phản biện: 10/12/2017 Ngày duyệt đăng: 15/12/2017 Southern Monitoring Center for Aquaculture Environment and Epidemics, Research Institute for Aquaculture No.2 *Email: lehongphuoc@yahoo.com TẠP CHÍ NGHỀ CÁ SƠNG CỬU LONG - SỐ 10 - THÁNG 12/2017 57 ... 12 /2017 VIỆN NGHIÊN CỨU NUÔI TRỒNG THỦY SẢN II OCCURRENCE OF WSSV, Vibrio parahaemolyticus CAUSING AHPND AND EHP IN POSTLARVAE AND EXTENSIVE-FARMED SHRIMP IN THE MEKONG DELTA, VIETNAM IN 2017. .. pathogens including WSSV, Vibrio parahaemolyticus and EHP by PCR technique In the dry season, the percentage of postlarvae samples positive with WSSV, Vibrio parahaemolyticus and EHP were 1.17%; 1.67%... WSSV, Vibrio parahaemolyticus and EHP were 8.08; 6.45% and 4.87%, respectively In the rainy season, the percentage of postlarvae samples positive with WSSV, Vibrio parahaemolyticus and EHP were