Nghiên cứu phân lập, giải trình tự và phân tích gen rps16 ở một số mẫu giống Nghệ (Curcuma longa L.) có hàm lượng Curcuminoids khác nhau (Khóa luận tốt nghiệp)

Nghiên cứu phân lập, giải trình tự và phân tích gen rps16 ở một số mẫu giống Nghệ (Curcuma longa L.) có hàm lượng Curcuminoids khác nhau (Khóa luận tốt nghiệp)Nghiên cứu phân lập, giải trình tự và phân tích gen rps16 ở một số mẫu giống Nghệ (Curcuma longa L.) có hàm lượng Curcuminoids khác nhau (Khóa luận tốt nghiệp)Nghiên cứu phân lập, giải trình tự và phân tích gen rps16 ở một số mẫu giống Nghệ (Curcuma longa L.) có hàm lượng Curcuminoids khác nhau (Khóa luận tốt nghiệp)Nghiên cứu phân lập, giải trình tự và phân tích gen rps16 ở một số mẫu giống Nghệ (Curcuma longa L.) có hàm lượng Curcuminoids khác nhau (Khóa luận tốt nghiệp)Nghiên cứu phân lập, giải trình tự và phân tích gen rps16 ở một số mẫu giống Nghệ (Curcuma longa L.) có hàm lượng Curcuminoids khác nhau (Khóa luận tốt nghiệp)Nghiên cứu phân lập, giải trình tự và phân tích gen rps16 ở một số mẫu giống Nghệ (Curcuma longa L.) có hàm lượng Curcuminoids khác nhau (Khóa luận tốt nghiệp)

(Curcuma longa L.) rps Chuyên ngành Khoa : Chính qui :C : CNSH&CNTP : 2011 - 2015 : L IC c k t qu c r t nhi u s ng d n t n tình c a th y, cô giáo, b c h t em xin g i l i c i Ban giám hi ng i h c Nông lâm Thái Nguyên, Ban ch nhi m khoa Công ngh sinh h c Công ngh th c ph m toàn th th nh ng ki n th t cho em t th i gian h c t p rèn luy n t i i h c Nông lâm Thái Nguyên Em xin bày t lòng bi TS Nguy c n th y TS Nguy ng d n em su t trình làm Khóa lu n cc B mơn Gi ng Công Ngh Sinh H c - Trung tâm Nghiên c u Tr ng Ch bi n thu c Hà N i - Vi n c Li u ki n thu n l em q trình làm khố lu n ng c Hóa phân tích tiêu chu n - Vi c Li ng khoa em trình phân tích m u ngh t i Khoa Và cu i cùng, v i t t c lòng kính tr ng bi n, em xin g i l ic ng viên t ng, ng l c cho em su t trình h c t p nghiên c u Em xin trân thành c Thái Nguyên, ngày tháng 06 Sinh viên Nguy n Th Hà DANH M C B NG X -1996 16 22 T 24 29 31 32 34 K 36 DANH M C HÌNH 11 -cisdiketone (1), s-trans-diketone (2), enol (3) 12 ortho methoxyl 15 ng chu ng Curcumin 31 Hình 4.2: K t qu n di s n ph m tách chi t DNA t ng s m u ngh 33 Hình 4.3: K t qu n di s n ph m PCR m u Ngh 35 Hình 4.4: K t qu tinh s ch s n ph m PCR m u Ngh 35 37 gen rps16 38 DANH M C CÁC CH VI T T T Bp base pair (c Cs C ng s DNA Deoxyribonucleic acid dNTPs deoxyribonucleotide triphosphates EDTA Ethylene diamine tetra acetate Kb Kilobase NST Nhi m s c th PCR polymerase chain reaction (ph n ng khu OD Optical density (M quang) Na2EDTA Ethylene diamine tetra acetate sodium TE Tris EDTA TAE Tris Acetate EDTA µl Micro litre µg Micro gram µM Micro Mole mM Mili Mole RNA ribonucleic acid RE Restriction enzyme (Enzym gi i h n) i gen) .3 Curcuma longa L.) Curcuminoids 11 .11 12 2.3 T Nam 16 16 19 22 .22 .22 23 23 23 23 .23 3.4.2 Thi .24 25 25 26 28 29 3.5.5 Phân tích gen rps16 30 PH N 4: K T QU VÀ TH O LU N 31 4.1 K t qu ng Curcumin c ngh .31 4.1.1 Xây d th chu ng Curcumin .31 ng Curcumin m u ngh .32 4.2 K t qu tách chi t DNA t ng s t m u nghiên c u 32 rps 35 4.4 K t qu tinh s ch s n ph m PCR 35 rps16 36 PH N 5: K T LU N VÀ KI N NGH 39 5.1 K t lu n .39 5.2 Ki n ngh 39 t t P 1.1 -30% [5] hóa Cây N Curcuma longa T N [5] tá tràng viêm loét, ung , da viru Curcumin c [2] cho Curcumin Curcumin [12] Các nghiên Curcumin Curcumin Trong phân tích gen rps longa L gh Curcuna Curcumin Curcumin g loài N vùng si : Curcuma longa L Curcumin 1.2 1.2 tiêu gen rps Curcuna longa L Curcumin 1.2.2 Y Curcuminoids phân tích - gen rps16 - rps Curcuminoids khác rps Curcuminoids rps Curcuminoids cao g Hình 4.5: rps16 - [21] - urcuminoids (0,34%) C18 (6,56 Hình 4.5 ng Hình 4.6 (A) (B) Hình 4.6: gen rps16 C18 C18 rps Trì hàm l C3, C4, C5, C6, C7, C10 (có Curcumin C1, C2, C8, C9, C11, C12, C13, C14, C15, C16, C17, C18, C19 (có hàm rps Curcumin rps có Curcumin AAAA Curcumin AAAAA rps Curcumin AAAA [20] PH N K T LU N VÀ KI N NGH 5.1 K t lu n Curcumin N nhóm: nhóm I (0,25- 0,62%): C3, C4, C5, C6, C7, C10; nhóm II cao (1,80- 8,26%): C1, C2, C8, C9, C11, C12, C13, C14, C15, C16, C17,C18 C19 c DNA t ng s c a 19 m u gen rps16 nh gen rps16 cho c ch th m u gi ng Ngh ng Curcuminoids th p AAAA Gen rps16 hà 5.2 Ki n ngh - Ti p t c phát tri gi ng Ngh - dài 270-271 bp th phân t t ch d c li u quý khác Vi t c li u an toàn hi u qu - Ti p t c thu th p thêm m u m i vùng trình t khác gen, l c l truy n gi a loài Ngh ch n l c m u ng cao ph c v cho s n xu t ng d ng nghiên c Nam m Vi nghiên c u thi t l p m i quan h di I công ngh Vùng Chu , 23/11/2009 Báo Thanh Hóa , , 12/12/2008 ,V , I: 8946 III 2) - 2008, trang 287 - 294 , trang 227- 230 10 11 , t 12 Aoi K., Kaburagi K., Seki T., Tobata T., Sarak M and Kuroyanagi M (1986) Studies on the cultivation of turmeric (Curcuma longa L.): Varietal differences in rhizome yield and Curcuminoid content, Bulletin of National Institute of Hygienic Sciences, (104): 124-128 13 Aggarwal S., Takada Y., Singh S., Myers J.N and Aggarwal B.B (2004) Inhibition of Growth and Survival of Human Head and Neck Squamous Cell Carcinoma Cells by Cur- cumin via Modulation of Nuclear Factorg, International Journal of Cancer,111(5): 679-692 14 Apavatjrut P., Anuntalabhochai S., Sirirugsa P., Alisi C (1999) Molecular Curcuma (Zingiberaceae) species Ann Bot 84, 529-534 15 Bharat B.A., Kumar A., Manoj S.A and Shishir S (2005) Curcumin Derived from Turmeric (Curcuma longa): a Spice for All Seasons, Phytopharmaceuticals in Cancer Chemopreventions, 350- 387 16 Chearwae W., Anuchapreedac S., Nandigama K., Ambudkar S.V and Limtrakul P.N (2004) Biochemical mechanism of modulation of human P-glycoprotein (ABCB1) by Curcumin I, II and III purified from Turmeric powder, Biochemical Pharmacology 17 Chen W.F., Deng S.L., Zhou B., Zang L., Liu Z.L (2005) Curcumin and its analogues as potent inhibitors of low density lipoprotein oxidation: Hatom abstraction from the phenolic groups and possible involvement of the 4-hydroxy-3 methoxyphenyl groups, National Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China 18 Coyne J.A and Orr H.A (2004) Patterns of Speciation in Evolution, Vol 43, (2): 362-381 19 Doyle JJ and Doyle J.L (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue Phytochemical Bulletin 19: 11-15 20 Hayakawa H., Kobayashi T., Minamiya Y., Ito K., Miyazaki A., Fukuda T., Yamamoto (2010) Molercular Indentification of Turmeric ( Curcuma longa, Zingiberaceae) with High Curcumin Content, Journal of Janpanese Botany, 85(5):263-269 21 Hayakawa H., Kobayashi T., Minaniya Y., Ito K , Miyazaki A., Fukuda T and Yamamoto Y (2011a), Development of a molecular marker to identify a candidate line of turmeric (Curcuma longa L.) with a high Curcumin content American Journal of Plant Sciences, (2): 15-26 22 Hayakawa H., Minaniya Y., Ito K., Yamamoto Y and Fukuda T (2011b) Difference of Curcumin content in Curcuma longa L (Zingiberaceae) caused by hybridization with other Curcuma species American Journal of Plant Sciences, (2): 111-119 23 Hergenhahn M., Soto U., Weninger A., Polack A., Hsu C H., Cheng, A.L and Rosl F., The chemopreventive compoud Curcumin is an efficient inhibitor of Epstein-Barr virus BL2F1 transcription in Raji DRLUC cells Mol Carcinogen., 2002, 33, 137-145 24 Heubl G (2010) New Aspects of DNA-based Authentication of Chinese Medicinal Plants by Molecular Biological Techniques, Planta Med 76: 1963-1974 25 Jayaprakasha G.K, Negi P.S., Anadharamakrishman C and Sakariah K.K (2001) Chemical composition of tumeric oil a hyproduct for from tumeric oleorsin inductry and Its inhibitory activity against different fungi Z Natuforsch., 56: 40-44 26 Kapoor L.D (1990) Handbook of Ayurvedic Medicinal Plants, CRC Press, Boca Raton, Florida, 185 27 Kalpana J., Warude P.C and Bhushan P (2004) Molecular markers in herbal drug technology Current Science, 87(2): 159-165 28 Lin J.K., Lin-Shia S.Y (2001) Mechanisms of cancer chemoprevention by Curcumin, Proc Natl Sci Counc Repub China B (25):59-66 29 Mahady G.B., Pendland S.L., Yun G And Lu Z.Z (2002) Tumeric (Curcuma longa L.) and Curcumin inhibit the growth of Helico-bacter pylori, of group carcinogen Anticancer Res.,( 22): 4179-4181 30 Masuda T., Maekawa T., Hidaka K., Bando H., Takeda Y and Yamagaguchi H (2001) Chemical studies on antioxidant mechanisms of Curcumin: analysis of oxidative coupling products from Curcumin nad linoleate, J.Agic Food Chem.(49), 2539-2547 31 Minami M., Nishio K., Ajioka Y., Kyushima H., Shigeki K., Kinjo K., Yamada K., Nagai M., Satoh K and Sakurai Y (2009) Identification of Curcuma plants and Curcumin content level by DNA Polymorphisms in the trnS-trnfM intergenic Spacer in Chloroplast DNA, Journal of Natural Medicines, 63, (1):75-79 32 Ravindran P.N., Babu K.N and Sivaranan K (2007) Turmeric: The Genus Curcuma Medicinal and Aromatic Plants Industrial Profles CRC Press, Boca Raton 33 Roughley P.J and Whiting D.A (1973) Experiments in the biosynthesis of Curcumin J Chem Soc., (20): 2379-2388 34 Ruby A.J., Kuttan G., Dinesh Babu K., Rajasekharan K.N and Kuttan R (1995) Antitumor and antioxidant activity of natural Curcuminoids Cancer Lett.,( 94), 79-83 35 Sasaki Y., Fushimi H., Cao H., Cai S.Q and Komatsu K (2002) Sequence Analysis of Chinese and Japanese Curcuma Drugs on the 18S rRNA Gene and trnK Gene and the Application of Amplification- Refractory Mutation System Analysis for Their Authentication Biological & Pharmaceutical Bulletin, 25, (12):1593-1599 36 Shen L and Ji H.F (2007), Theoretical study on physicochemical properties of Curcumin, Spectrochimica Acta Part A: Molecular and Biomolecular Spetroscopy, (67): 619-623 37 Sigrist M.S., Pinheiro J.B., Filho J.A and Zucchi M.I (2011) Genetic diversity of turmeric germplasm (Curcuma longa; Zingiberaceae) identifed by microsatellite markers Genetics and Molecular Research, 10 (1): 419-428 38 Srivatsava S and Nidhi M (2009) Genetic Markers - A Cutting Edge Technology in Herbal Drug Research Journal of Chemical and Pharmaceutical Research 1(1): 1-18 39 Tharachand C., Immanuel S.C and Mythili M.N (2012) Molecular markers in characterization of medicinal plants: An overview Research in Plant Biology, 2(2): 01-12 rps16 rps >1849327_Rps16_1 Rps16_F_ CCCCTAGAAACGTATAGGAGGTTTTATCCTCATACGGCTCGAACTC GAGAAAAATTTTTCATTCGTACTCATAACTCAAGTTGGGTAATTCT GACATAGTCCCAGGGGAATCCTTAAACATTTATTGAGCCGTCTCTA ACCTCTTTTGTTTGTCTCATCCCGAGTCAATTATTCTGATCCCTTTC TTGAGACAATTGAAAATAGTGTTTCCTTGTTCCGGAATCCTTTATC TTTCCTTTGTAAAATCATTGGGTTTAGAACATTACTTCGA rps >1849328_Rps16_2 Rps16_F_ CCCCCTAGAAACGTATAGGAGGTTTTATCCTCATACGGCTCGAACT CGAGAAAAATTTTTCATTCGTACTCAAACTCAAGTTGGGTAATTCT GACATAGTCCCAGGGGAATCCTTAAACATTTATTGAGCCGTCTCTA ACCTCTTTTGTTTGTCTCATCCCGAGTCAATTATTCTGATCCCTTTC TTGAGACAATTGAAAATAGTGTTTCCTTGTTCCGGAATCCTTTATC TTTCCTTTGTAAAATCATTGGGTTTAGAACATTACTTCGA rps >1849329_Rps16_3 Rps16_F_ CCCCCTAGAAACGTATAGGAGGTTTTATCCTCATACGGCTCGAACT CGAGAAAATTTTTCATTCGTACTCATAACTCAAGTTGGGTAATTCT GACATAGTCCCAGGGGAATCCTTAAACATTTATTGAGCCGTCTCTA ACCTCTTTTGTTTGTCTCATCCCGAGTCAATTATTCTGATCCCTTTC TTGAGACAATTGAAAATAGTGTTTCCTTGTTCCGGAATCCTTTATC TTTCCTTTGTAAAATCATTGGGTTTAGAACATTACTTCGA rps >1849330_Rps16_4 Rps16_F_ CCCCCTAGAAACGTATAGGAGGTTTTATCCTCATACGGCTCGAACT CGAGAAAATTTTTCATTCGTACTCATAACTCAAGTTGGGTAATTCT GACATAGTCCCAGGGGAATCCTTAAACATTTATTGAGCCGTCTCTA ACCTCTTTTGTTTGTCTCATCCCGAGTCAATTATTCTGATCCCTTTC TTGAGACAATTGAAAATAGTGTTTCCTTGTTCCGGAATCCTTTATC TTTCCTTTGTAAAATCATTGGGTTTAGAACATTACTTCGA rps >1849331_Rps16_5 Rps16_F_ CCCCCTAGAAACGTATAGGAGGTTTTATCCTCATACGGCTCGAACT CGAGAAAATTTTTCATTCGTACTCATAACTCAAGTTGGGTAATTCT GACATAGTCCCAGGGGAATCCTTAAACATTTATTGAGCCGTCTCTA ACCTCTTTTGTTTGTCTCATCCCGAGTCAATTATTCTGATCCCTTTC TTGAGACAATTGAAAATAGTGTTTCCTTGTTCCGGAATCCTTTATC TTTCCTTTGTAAAATCATTGGGTTTAGAACATTACTTCGA en rps >1849332_Rps16_6 Rps16_F_ CCCCCTAGAAACGTATAGGAGGTTTTATCCTCATACGGCTCGAACT CGAGAAAATTTTTCATTCGTACTCATAACTCAAGTTGGGTAATTCT GACATAGTCCCAGGGGAATCCTTAAACATTTATTGAGCCGTCTCTA ACCTCTTTTGTTTGTCTCATCCCGAGTCAATTATTCTGATCCCTTTC TTGAGACAATTGAAAATAGTGTTTCCTTGTTCCGGAATCCTTTATC TTTCCTTTGTAAAATCATTGGGTTTAGAACATTACTTCGA rps >1849333_Rps16_7 Rps16_F_ CCCCCTAGAAACGTATAGGAGGTTTTATCCTCATACGGCTCGAACT CGAGAAAATTTTTCATTCGTACTCATAACTCAAGTTGGGTAATTCT GACATAGTCCCAGGGGAATCCTTAAACATTTATTGAGCCGTCTCTA ACCTCTTTTGTTTGTCTCATCCCGAGTCAATTATTCTGATCCCTTTC TTGAGACAATTGAAAATAGTGTTTCCTTGTTCCGGAATCCTTTATC TTTCCTTTGTAAAATCATTGGGTTTAGAACATTACTTCGA rps >1849334_Rps16_8 Rps16_F_ CCCCCTAGAAACGTATAGGAGGTTTTATCCTCATACGGCTCGAACT CGAGAAAAATTTTTCATTCGTACTCATAACTCAAGTTGGGTAATTC TGACATAGTCCCAGGGGAATCCTTAAACATTTATTGAGCCGTCTCT AACCTCTTTTGTTTGTCTCATCCCGAGTCAATTATTCTGATCCCTTT CTTGAGACAATTGAAAATAGTGTTTCCTTGTTCCGGAATCCTTTAT CTTTCCTTTGTAAAATCATTGGGTTTAGAACATTACTTCGA rps 9) >1849335_Rps16_9 Rps16_F_ CCCCCTAGAAACGTATAGGAGGTTTTATCCTCATACGGCTCGAACT CGAGAAAAATTTTTCATTCGTACTCATAACTCAAGTTGGGTAATTC TGACATAGTCCCAGGGGAATCCTTAAACATTTATTGAGCCGTCTCT AACCTCTTTTGTTTGTCTCATCCCGAGTCAATTATTCTGATCCCTTT CTTGAGACAATTGAAAATAGTGTTTCCTTGTTCCGGAATCCTTTAT CTTTCCTTTGTAAAATCATTGGGTTTAGAACATTACTTCGA rps >1849336_Rps16_10 Rps16_F_ CCCCCTAGAAACGTATAGGAGGTTTTATCCTCATACGCGTCGAACT CGAGAAAATTTTTCATTCTTACTCTTAACTCAAGTTGGGTAATTCT GACATAGTCCCAGGGGAATCCTTAAACATTTATTGAGCCGTCTCTA ACCTCTTTTGTTTGTCTCATCCCGAGTCAATTATTCTGATCCCTTTC TTGAGACAATTGAAAATAGTGTTTCCTTGTTCCGGAATCCTTTATC TTTCCTTTGTAAAATCATTGGGTTTAGAACATTACTTCGA rps >1849337_Rps16_11 Rps16_F_ CCCCCTAGAAACGTATAGGAGGTTTTATCCTCATACGGCTCGAACT CGAGAAAAATTTTTCATTCGTACTCATAACTCAAGTTGGGTAATTC TGACATAGTCCCAGGGGAATCCTTAAACATTTATTGAGCCGTCTCT AACCTCTTTTGTTTGTCTCATCCCGAGTCAATTATTCTGATCCCTTT CTTGAGACAATTGAAAATAGTGTTTCCTTGTTCCGGAATCCTTTAT CTTTCCTTTGTAAAATCATTGGGTTTAGAACATTACTTCGA rps >1849338_Rps16_12 Rps16_F_ CCCCCTAGAAACGTATAGGAGGTTTTATCCTCATACGGCTCGAACT CGAGAAAAATTTTTCATTCGTACTCATAACTCAAGTTGGGTAATTC TGACATAGTCCCAGGGGAATCCTTAAACATTTATTGAGCCGTCTCT AACCTCTTTTGTTTGTCTCATCCCGAGTCAATTATTCTGATCCCTTT CTTGAGACAATTGAAAATAGTGTTTCCTTGTTCCGGAATCCTTTAT CTTTCCTTTGTAAAATCATTGGGTTTAGAACATTACTTCGA rps >1849339_Rps16_13 Rps16_F_ CCCCCTAGAAACGTATAGGAGGTTTTATCCTCATACGGCTCGAACT CGAGAAAAATTTTTCATTCGTACTCATAACTCAAGTTGGGTAATTC TGACATAGTCCCAGGGGAATCCTTAAACATTTATTGAGCCGTCTCT AACCTCTTTTGTTTGTCTCATCCCGAGTCAATTATTCTGATCCCTTT CTTGAGACAATTGAAAATAGTGTTTCCTTGTTCCGGAATCCTTTAT CTTTCCTTTGTAAAATCATTGGGTTTAGAACATTACTTCGA rps >1849340_Rps16_14 Rps16_F_ CCCCCTAGAAACGTATAGGAGGTTTTATCCTCATACGGCTCGAACT CGAGAAAAATTTTTCATTCGTACTCATAACTCAAGTTGGGTAATTC TGACATAGTCCCAGGGGAATCCTTAAACATTTATTGAGCCGTCTCT AACCTCTTTTGTTTGTCTCATCCCGAGTCAATTATTCTGATCCCTTT CTTGAGACAATTGAAAATAGTGTTTCCTTGTTCCGGAATCCTTTAT CTTTCCTTTGTAAAATCATTGGGTTTAGAACATTACTTCGA rps >1849341_Rps16_15 Rps16_F_ CCCCCTAGAAACGTATAGGAGGTTTTATCCTCATACGGCTCGAACT CGAGAAAAATTTTTCATTCGTACTCATAACTCAAGTTGGGTAATTC TGACATAGTCCCAGGGGAATCCTTAAACATTTATTGAGCCGTCTCT AACCTCTTTTGTTTGTCTCATCCCGAGTCAATTATTCTGATCCCTTT CTTGAGACAATTGAAAATAGTGTTTCCTTGTTCCGGAATCCTTTAT CTTTCCTTTGTAAAATCATTGGGTTTAGAACATTACTTCGA rps >1849342_Rps16_16 Rps16_F_ CCCCCTAGAAACGTATAGGAGGTTTTATCCTCATACGGCTCGAACT CGAGAAAAATTTTTCATTTCGTACTCATAACTCAAGTTGGGTAATT CTGACATAGTCCCAGGGGAATCCTTAAACATTTATTGAGCCGTCTC TAACCTCTTTTGTTTGTCTCATCCCGAGTCAATTATTCTGATCCCTT TCTTGAGACAATTGAAAATAGTGTTTCCTTGTTCCGGAATCCTTTA TCTTTCCTTTGTAAAATCATTGGGTTTAGAACATTACTTCGA rps >1849343_Rps16_17 Rps16_F_ CCCCCTAGAAACGTATAGGAGGTTTTATCCTCATACGGCTCGAACT CGAGAAAAATTTTTCATTCGTACTCATAACTCAAGTTGGGTAATTC TGACATAGTCCCAGGGGAATCCTTAAACATTTATTGAGCCGTCTCT AACCTCTTTTGTTTGTCTCATCCCGAGTCAATTATTCTGATCCCTTT CTTGAGACAATTGAAAATAGTGTTTCCTTGTTCCGGAATCCTTTAT CTTTCCTTTGTAAAATCATTGGGTTTAGAACATTACTTCGA rps >1849344_Rps16_18 Rps16_F_ (4-5A) CCCCCTAGAAACGTATAGGAGGTTTTATCCTCATACGGCTCGAACT CGAGAAAAATTTTTCATTCGTACTCATAACTCAAGTTGGGTAATTC TGACATAGTCCCAGGGGAATCCTTAAACATTTATTGAGCCGTCTCT AACCTCTTTTGTTTGTCTCATCCCGAGTCAATTATTCTGATCCCTTT CTTGAGACAATTGAAAATAGTGTTTCCTTGTTCCGGAATCCTTTAT CTTTCCTTTGTAAAATCATTGGGTTTAGAACATTACTTCGA rps >1849345_Rps16_19 Rps16_F_ CCCCCTAGAAACGTATAGGAGGTTTTATCCTCATACGGCTCGAACT CGAGAAAAATTTTTCATTCGTACTCATAACTCAAGTTGGGTAATTC TGACATAGTCCCAGGGGAATCCTTAAACATTTATTGAGCCGTCTCT AACCTCTTTTGTTTGTCTCATCCCGAGTCAATTATTCTGATCCCTTT CTTGAGACAATTGAAAATAGTGTTTCCTTGTTCCGGAATCCTTTAT CTTTCCTTTGTAAAATCATTGGGTTTAGAACATTACTTCGA rps - Thái Nguyên, ngày ... Curcuma longa L Curcumin 1.2 1.2 tiêu gen rps Curcuna longa L Curcumin 1.2.2 Y Curcuminoids phân tích - gen rps16 - rps Curcuminoids khác rps Curcuminoids rps Curcuminoids cao g 2.1 Curcuma longa. .. C longa loài chi Curcuma C longa Curcumin Sasaki cs (2002) [35] vùng gene 18S rRNA, Cao cs gen trn Curcumin Minani cs (2008) [31] Curcuma g loài C longa Curcumin trnS - trnfM gene rps phân tích. .. Ninh Bình ch Thành, Thanh Hóa Kon Tum, Kon Tum 3.1.2 ích gen rps16 rps16 - : 12/2014- 5/2015 - Thu th Curcuminoids rps16 + gen + - e e rps16 3.4.1 Tris-HCl, 20 mM EDTA, 2% PVP (w/v), 2% - -Mercaptoethanol