Nghiên cứu phân lập, giải trình tự và phân tích gen rpl16 ở một số mẫu giống nghệ (Curcuna longa L.) có hàm lượng curcuminoids khác nhau (Khóa luận tốt nghiệp)Nghiên cứu phân lập, giải trình tự và phân tích gen rpl16 ở một số mẫu giống nghệ (Curcuna longa L.) có hàm lượng curcuminoids khác nhau (Khóa luận tốt nghiệp)Nghiên cứu phân lập, giải trình tự và phân tích gen rpl16 ở một số mẫu giống nghệ (Curcuna longa L.) có hàm lượng curcuminoids khác nhau (Khóa luận tốt nghiệp)Nghiên cứu phân lập, giải trình tự và phân tích gen rpl16 ở một số mẫu giống nghệ (Curcuna longa L.) có hàm lượng curcuminoids khác nhau (Khóa luận tốt nghiệp)Nghiên cứu phân lập, giải trình tự và phân tích gen rpl16 ở một số mẫu giống nghệ (Curcuna longa L.) có hàm lượng curcuminoids khác nhau (Khóa luận tốt nghiệp)Nghiên cứu phân lập, giải trình tự và phân tích gen rpl16 ở một số mẫu giống nghệ (Curcuna longa L.) có hàm lượng curcuminoids khác nhau (Khóa luận tốt nghiệp)
Trang 1: Chính quy Chuyên ngành
: 2011 - 2015
Trang 2CÓ HÀM
: Chính quy Chuyên ngành
: K43 - CNSH : 2011 - 2015
êm
Trang 3tích gen rpl Curcuna longa L. ng
i
-Sinh viên
Trang 413
rpl16 19
19
19
22
23
25
rpl 34
Trang 55
8
9
22
24
25
26
rpl16 27
28
Hình 4.8 rpl 36
Trang 6PCR polymerase chain reaction
RNA ribonucleic acid
Trang 71
2
2
3
3
3
3
4
4
4
5
6
2.1 6
8
8
8
8
11
11
13
13
13
14
14
14
Trang 814
15
15
15
17
18
® ExpinTM 20
3 .21
22
22
22
23
rpl1624 24
27
36
37
5.1 .37
37
38
Trang 10(Kalpana et al 2004)[14].
rpl Curcuna longa L.
Trang 12xanhthorrhiza R Curcuma aeruginosa R.).
1,5
lòng máng, dài
Trang 14sabinen (0,6%), cineol (1%), borneol (l0,5%;) zingi, beren (25%),
Trang 15TNV-999-vàng do X
, Hoàng , 2008)[2]
Trang 162.2.3
Trang 17et al 2002)[25] ( Ravindran et al 2007)[22]
Trang 18(2-amidinopropane hydrochloride) (AAPH), Cu2+
phenolic:1,7-bis(3,4-dimethoxyphenyl)-1,6-heptadiene 3,5-dione (1), methoxyphenyl)-1,6-heptadiene-3,5-dione (2) và 1,7- diphenyl-1,6-heptadiene-3,5-
Trang 20là
-1, HIV-1-RT
Hayakawa et al (2011a)[9],
các loài trong chi Curcuma
Trang 24- o:
UV-1800
n (mg/ml)
Trang 27rpl C.longa
Trang 28µl+
Trang 32
gen rpl16
Doyle & Doyle (1978)
lý RNase,
tách
Hình 4.2
N15.
o
(Hình 4.3)
Trang 35rpl rpl16 F và rpl16
rpl16 F, rpl16
PCR
®ExpinTM
8% (Hình 4.6)
Trang 36GTTTCTTCTCATCCAGCTCCATGACATATTACAGATACACACGGAAAAAATAATCCAATAAGACATTTATCAATATTGAATTTGTTATATAGGAAGATGTATGTACGGGATATACAGATAGAATGTTTCTATTATGCATATTTATGGATTATAGATAATGTTTATAATGTTTTTTTATAATGATCCTTTTTTTGACCCTTCTCAAAGGATGCCAAAATATTGTAATGTAATCTAAAGTTTCGCGGGCGAATATTGACTCTTTC (264 bp)
rpl
GTTTCTTCTCATCCAGCTCCATGACATATTACAGATACACACGGAAAAAATAATCCAATAAGACATTTATCAATATTGAATTTGTTATATAGGAAGATGTATGTACGGGATATACAGATAGAATGTTTCTATTATGCATATTTATGGATTATAGATAATGTTTATAATGTTTTTTTATAATGATCCTTTTTTTGACCCTTCTCAAAGGATGCCAAAATATTGTAATGTAATCTAAAGTTTCGCGGGCGAATATTGACTCTTTC
rpl
Trang 37AATAATCCAATAAGACATTTATCAATATTGAATTTGTTATATAGGAAGATGTATGTACGGGATATACAGATAGAATGTTTCTATTATGCATATTTATGGATTATAGATAATGTTTATAATGTTTTTTTATAATGATCCTTTTTTTGACCCTTCTCAAAGGATGCCAAAATATTGTAATGTAATCTAAAGTTTCGCGGGCGAATATTGACTCTTTC (262 bp)
rpl
GTTTCTTCTCATCCAGCTCCATGACATATTACAGATACACACGGAAAATAATCCAATAAGACATTTATCAATATTGAATTTGTTATATAGGAAGATGTATGTACGGGATATACAGATAGAATGTTTCTATTATGCATATTTATGGATTATAGATAATGTTTATAATGTTTTTTTATAATGATCCTTTTTTTGACCCTTCTCAAAGGATGCCAAAATATTGTAATGTAATCTAAAGTTTCGCGGGCGAATATTGACTCTTTC
rpl
GTTTCTTCTCATCCAGCTCCATGACATATTACAGATACACACGGAAAATAATCCAATAAGACATTTATCAATATTGAATTTGTTATATAGGAAGATGTATGTACGGGATATACAGATAGAATGTTTCTATTATGCATATTTATGGATTATAGATAATGTTTATAATGTTTTTTTATAATGATCCTTTTTTTGACCCTTCTCAAAGGATGCCAAAATATTGTAATGTAATCTAAAGTTTCGCGGGCGAATNATTGACTCTTTC
rpl
GTTTCTTCTCATCCAGCTCCATGACATATTACAGATACACACGGAAAATAATCCAATAAGACATTTATCAATATTGAATTTGTTATATAGGAAGATGTATGTACGGGATATACAGATAGAATGTTTCTTTATGCATATTTATGGATTATAGATAATGTTTATAATGTTTTTTTATAATGATCCTTTTTTTGACCCTTCTCAAAGGATGCCAAAATATTGTAATGTAATCTAAAGTTTCGCGGGCGAATATTGACTCTTTC
Trang 38GTTTCTTCTCATCCAGCTCCATGACATATTACAGATACACACGGAAAATAATCCAATAAGACATTTATCAATATTGAATTTGTTATATAGGAAGATGTATGTACGGGATATACAGATAGAATGTTTCTTTATGCATATTTATGGATTATAGATAATGTTTATAATGTTTTTTTATAATGATCCTTTTTTTGACCCTTCTCAAAGGATGCCAAAATATTGTAATGTAATCTAAAGTTTCGCGGGCGAATATTGACTCTTTC
GTTTCTTCTCATCCAGCTCCATGACATATTACAGATACACACGGAAAAAATAATCCAATAAGACATTTATCAATATTGAATTTGTTATATAGGAAGATGTATGTACGGGATATACAGATAGAATGTTTCTATTATGCATATTTATGGATTATAGATAATGTTTATAATGTTTTTTTATAATGATCCTTTTTTTGACCCTTCTCAAAGGATGCCAAAATATTGTAATGTAATCTAAAGTTTCGCGGGCGATAATTGACTCTTTC
rpl
GTTTCTTCTCATCCAGCTCCATGACATATTACAGATACACACGGAAAAAATAATCCAATAAGACATATTATCAATATTGAATTTGTTATATAGGAAGATGTATGTACGGGATATACAGATAGAATGTTTCTATTATGCATATTTATGGATTATAGATAATGTTTATAATGTTTTTTTATAATGATCCTTTTTTTGACCCTTCTCAAAGGATGCCAAAATATTGTAATGTAATCTAAAGTTTCGCGGGCGAAATATTGACTCTTTC
rpl
GTTTCTTCTCATCCAGCTCCATGACATATTACAGATACACACGGAA
AATAATCCATAAGACATTTATCAATATTGAATTTGTTATATAGGAAGATGTATGTACGGGATATACAGATAGAATGTTTCTATTATGCATATTTATGGATTATAGATAATGTTTATAATGTTTTTTTATAATGATCCTT
Trang 39rpl
GTTTCTTCTCATCCAGCTCCATGACATATTACAGATACACACGGAAAAAATAATCCAATAAGACATTTATCAATATTGAATTTGTTATATAGGAAGATGTATGTACGGGATATACAGATAGAATGTTTCTATTATGCATATTTATGGATTATAGATAATGTTTATAATGTTTTTTTATAATGATCCTTTTTTTGACCCTTCTCAAAGGATGCCAAAATATTGTAATGTAATCTAAAGTTTCGCGGGCGAATTTGGACTCTTTC
rpl
GTTTCTTCTCATCCAGCTCCATGACATATTACAGATACACACGGAAAAAATAATCCAATAAGACATTTATCAATATTGAATTTGTTATATAGGAAGATGTATGTACGGGATATACAGATAGAATGTTTCTATTATGCATATTTATGGATTATAGATAATGTTTATAATGTTTTTTTATAATGATCCTTTTTTTGACCCTTCTCAAAGGATGCCAAAATATTGTAATGTAATCTAAAGTTTCGCGGGCGAATATTGACTCTTTC
rpl
GTTTCTTCTCATCCAGCTCCATGACATATTACAGATACACACGGAAAAAATAATCCAATAAGACATTTATCAATATTGAATTTGTTATATAGGAAGATGTATGTACGGGATATACAGATAGAATGTTTCTATTATGCATATTTATGGATTATAGATAATGTTTATAATGTTTTTTTATAATGATCCTTTTTTTGACCCTTCTCAAAGGATGCCAAAATATTGTAATGTAATCTAAAGTTTCGCGGGCGAATATTGACTCTTTC
GTTTCTTCTCATCCAGCTCCATGACATATTACAGATACACACGGAAAAAATAATCCAATAAGACATTTATCAATATTGAATTTGTTATATAG
Trang 40rpl
GTTTCTTCTCATCCAGCTCCATGACATATTACAGATACACACGGAAAAAATAATCCAATAAGACATTTATCAATATTGAATTTGTTATATAGGAAGATGTATGTACGGGATATACAGATAGAATGTTTCTATTATGCATATTTATGGATTATAGATAATGTTTATAATGTTTTTTTATAATGATCCTTTTTTTGACCCTTCTCAAAGGATGCCAAAATATTGTAATGTAATCTAAAGTTTCGCGGGCGAATATTGNACTCTTTC
rpl
GTTTCTTCTCATCCAGCTCCATGACATATTACAGATACACACGGAAAAAATAATCCAATAAGACATTTATCAATATTGAATTTGTTATATAGGAAGATGTATGTACGGGATATACAGATAGAATGTTTCTATTATGCATATTTATGGATTATAGATAATGTTTATAATGTTTTTTTATAATGATCCTTTTTTTGACCCTTCTCAAAGGATGCCAAAATATTGTAATGTAATCTAAAGTTTC
rpl
GTTTCTTCTCATCCAGCTCCATGACATATTACAGATACACACGGAAAAAATAATCCAATAAGACATTTATCAATATTGAATTTGTTATATAGGAAGATGTATGTACGGGATATACAGATAGAATGTTTCTATTATGCATATTTATGGATTATAGATAATGTTTATAATGTTTTTTTATAATGATCCTTTTTTTGACCCTTCTCAAAGGATGCCAAAATATTGTAATGTAATCTAAAGTTTCGCGGGCGAATATTGGACTCTTTC
rpl
Trang 41curc
Trang 462 Aoi K., Kaburagi K., Seki T., Tobata T., Sarak M and Kuroyanagi M
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