phân tích trình tự ITS và matK cây họ Gừng Zingiberaceae

luận văn nói về quá trình phân tích trình tự gen ITS và matK để phân loại bằng Sử dụng phương pháp PCR – RAPD khảo sát đa dạng di truyền,dựng cây phát sinh loài dựa vào trình tự DNA vùng ITS, vùng trnK – matK và kết hợp vùng ITS và trnK – matK

-i- MỤC LỤC - ii - DANH MỤC CHỮ KÍ HIỆU, CHỮ VIẾT TẮT AFLP AP - PCR bp cpDNA CTAB DAF DNA DNase dNTPs EDTA ISSR ITS matK Mega ML Amplified fragment length polymorphism Arbitrary primed polymerase chain reaction base pair Chloroplast geneome, gene lục lạp Cetyl trimethyl ammonium bromide DNA amplification fingerprinting Deoxyribonucleic acid Deoxyribonuclease Deoxynucleotide triphosphate Ethylenediamine tetraacetate Inter simple sequence repeat Internal transcribed spacer maturaseK, gene mã hóa cho megakaryocyte - associated tyrosine kinase Molecular evolutionary genetics analysis Maximum likelihood MP Maximum parsimony mtDNA nDNA NJ nrDNA NTSYSpc OD ORF OTU PCR PVP RAPD rDNA RFLP RNA Rnase rRNA SM SSR TAE TE ts ts/tv tv trnK Mitochondrial DNA, DNA ti thể Nuclear DNA, DNA nhân Neighbour joining Nuclear ribosomal DNA, DNA ribosome nhân Numerical taxonomy and multivariate analysis system for personal computer Optical density Open reading frame, khung đọc mở Operational taxonomic units Polymerase chain reaction Polyvinylpyrrolidone Random amplification of polymorphic DNA Ribosomal DNA Restriction fragment length polymorphism Ribonucleic acid Ribonuclease Ribosomal ribonucleic acid Simple matching coefficient Simple sequence repeat Tris – acetic acid – EDTA Tris- HCl + EDTA Transition, đồng hoán Transition/transversion, tỉ lệ đồng hoán / dị hoán Transversion, dị hoán Translocated tRNA-Lys gene - iii - U UPGMA UV V VNTR Unit Unweighted pair group method with arithmetic mean Ultraviolet Volt Variable number tandem repeat - iv - DANH MỤC BẢNG -v- DANH MỤC HÌNH VẼ, ĐỒ THỊ Hình 1.1 Các vùng gene dùng phân tích phân loại thực vật mức độ khác [33] Hình 1.2 Sơ đồ vùng ITS [37] 11 Hình 1.3 Sơ đồ mô tả phương pháp dùng để định danh thực vật mức loài [33] .13 Hình 1.4 Sơ đồ mã vạch DNA đề cử để xác định lồi thực vật [10] 14 Hình 2.1 Sơ đồ mơ tả vị trí mồi dùng giải trình tự đoạn ITS 24 Hình 2.2 Sơ đồ mơ tả vị trí mồi dùng giải trình tự đoạn trnK .25 Hình 3.1 Sơ đồ thể mối tương quan di truyền 25 mẫu ngải sở kiểu gene 31 Hình 3.2 Sản phẩm khuyếch đại vùng ITS M: thang 100 bp 33 Hình 3.3 Sản phẩm khuyếch đại vùng trnK M: thang Kb .35 39 Hình 3.4 Cây phát sinh lồi có gốc trình tự ITS Giá trị bootstrap từ trái qua phải: ML / MP / NJ Mơ hình tiến hóa theo Akaike Information Criterion: TrNef+G Phần chữ in đậm trình tự -lnL = 2733.7436 39 41 Hình 3.5 Cây phát sinh lồi có gốc trình tự matK Giá trị bootstrap từ trái qua phải: ML /MP /NJ Mơ hình tiến hóa theo Akaike Information Criterion: TPM1uf+G Phần chữ in đậm trình tự -lnL = 3737.1652 41 43 Hình 3.6 Cây phát sinh lồi có gốc kết hợp trình tự ITS matK Giá trị bootstrap từ trái qua phải: ML /MP /NJ Mơ hình tiến hóa theo Akaike Information Criterion: GTR+I+G Phần chữ in đậm trình tự -lnL = 6832.0743 .43 -1- MỞ ĐẦU Họ Gừng (Zingiberaceae) Việt Nam phong phú, có từ 17 đến 20 chi 100 loài [3] Các họ Gừng sử dụng từ lâu đời, làm gia vị làm thuốc, phận dùng thường thân rễ Với phát triển khoa học, người ta nghiên cứu chứng minh tác dụng dược lý quý giá họ Gừng Các nghiên cứu ban đầu thuốc có khả chữa ung thư Việt Nam cho thấy có 50 lồi thuộc 36 chi 24 họ có khả chữa ung thư, Zingiberaceae đa dạng (16%) [8] Ngồi ra, nghiên cứu sàng lọc tác dụng kháng ung thư 77 thuốc Việt Nam thu thập từ vùng Bảy Núi, An Giang Lâm Đồng cho thấy 15 dịch chiết có khả kháng ung thư số dòng tế bào, có lồi nằm chi Alpinia thuộc họ Gừng [43] Ở vùng Bảy Núi, bên cạnh gừng, nghệ, riềng, đồng bào trồng sử dụng số ngải thuộc họ Gừng để chữa chứng đau bụng khí, viêm đại tràng, bó chữa trật chân Thực tế lồi ngải nói riêng, họ Gừng nói chung phong phú, lại có đặc điểm hình thái giống nhau, phân bố vùng khác với tên gọi khác nhau, nên dựa vào đặc điểm hình thái thực vật, vi phẫu, hố học (kiểu hình) chưa đủ để xác định, dễ bị nhầm lẫn Để góp phần xây dựng sở liệu phục vụ công tác bảo tồn nguồn gene phát triển nguồn dược liệu nhiều tiềm này, tiến hành đề tài khảo sát đa dạng di truyền số thuốc họ Gừng (Zingiberaceae) vùng Bảy Núi – An Giang Nội dung nghiên cứu đề tài: Sử dụng phương pháp PCR – RAPD khảo sát đa dạng di truyền Xây dựng phát sinh lồi dựa vào trình tự DNA vùng ITS, vùng trnK – matK kết hợp vùng ITS trnK – matK Bước đầu định danh mẫu ngải dựa vào trình tự ITS matK Mở đầu -2- CHƯƠNG TỔNG QUAN 1.1 Khái quát họ Gừng (Zingiberaceae) 1.1.1 Đặc điểm hình thái Các họ Gừng (Zingiberaceae) gồm thân thảo sống lâu năm Rễ nhỏ, hình sợi; đơi đầu rễ phình to lên thành dạng củ Thân rễ to, nạc, nằm ngang, chứa nhiều chất dự trữ, có ngắn mang hoa Các bẹ ôm chặt lấy tạo thành thân giả, ngắn (Distichochlamys, Kaempferia…) hay cao - m, đơi cao tới - m (Alpinia, Amomum…), không phân nhánh Cây thường có mùi thơm mùi hắc số loài chi Zingiber Lá đơn, mọc cách, xếp thành hai hàng, thường hướng lên trên, nằm ngang gần song song với mặt đất (Kaempferia galanga, K pulchra); có bẹ dạng vảy Lá gồm phần: bẹ lá, cuống lá, lưỡi phiến Bẹ mở đến gốc, phần bẹ thường ôm chặt lấy thành thân giả Cuống có hay khơng có, ngắn hay dài (có thể dài tới 25 cm), hình lịng máng nơng sâu Lưỡi (thìa lá) phần bẹ cuống lá, từ bẹ kéo dài lên Lưỡi dày hay mỏng dạng màng, đầu nguyên hay xẻ hai, cụt ngang, dài - mm tới vài cm Phiến lá: hình mác, hình trứng hẹp, bầu dục, gần trịn (K pulchra), gốc phiến nhọn, hình nêm hay gần trịn; đầu phiến thường nhọn, đơi thót nhỏ thành dạng đi, trịn Thơng thường phiến màu xanh, vài loài số chi mặt có đốm trắng loang lổ (Stahlianthus) hay dọc gân mặt nâu đỏ (Curcuma) mặt nâu đỏ (Distichochlamys, Stahlianthus, Zingiber) Cụm hoa mọc thân có hay từ thân rễ sát mặt đất, tách biệt với thân có lá, từ bẹ Cụm hoa dạng chùy, chùm hay Cuống cụm hoa mọc từ thân rễ số chi bao phủ bẹ dạng vảy thưa hay dày Cụm hoa thường không phân nhánh, trừ số lồi chi Globba, Alpinia, Elettaria, Elettariopsis [2] Mở đầu -2- 1.1.2 Phân bố Họ Gừng phân bố chủ yếu vùng nhiệt đới Nó tìm thấy vùng nhiệt đới châu Á, châu Phi châu Mỹ; đặc biệt phân bố rộng rãi vùng Đơng Nam Á Một chi tìm thấy Trung Nam Mỹ Renealmia; bốn chi Aframomum, Aulotandra, Siphonochilus, Renealmia tìm thấy Châu Phi; chi cịn lại phân bố vùng Đơng Á đảo thuộc Thái Bình Dương [23] Bảng 1.1 trình bày phân bố họ Gừng châu Á, vùng Đơng Dương có tới 120 lồi (số liệu năm 1998) [40] Bảng 1.1 Phân bố họ Gừng Châu Á [40] Vùng địa lý Thế giới (tổng cộng) Trung Quốc Ấn Độ Đông Dương Malaysia Nepal Philippines Thái Lan Chi 52 21 18 14 25 11 15 20 Loài 1500 200 120 120 650 35 103 200 1.1.3 Giá trị sử dụng Trong Gừng (Zingiberales) Việt Nam, họ Gừng (Zingiberaceae) họ có số lượng lồi nhiều Đây họ có nhiều đại diện có giá trị làm thuốc chữa bệnh, nhiều loài sử dụng làm gia vị, làm cảnh [2] Các thuộc họ Gừng sử dụng làm thuốc theo thuốc dân gian Riềng (Alpinia officinarum Han.) dùng làm gia vị làm thuốc Nghệ (Curcuma domestica-Val hay C Longa L.) có thân rễ làm gia vị, làm thuốc chữa bệnh dày, bệnh vàng da, dùng cho phụ nữ sau sinh đẻ Gừng (Zingiber officinale Rosc.) có thân rễ thơm cay, dùng làm gia vị, làm mứt làm thuốc, có tác dụng hưng phấn, dễ tiêu Gừng gió (Z zerumbet Sm ex L.) có thân rễ vị đắng cay, dùng làm thuốc Ở rừng Việt Nam, gặp số mọc tầng thấp Ré (Alpinia speciosa K Chum.) có cánh mơi vàng viền đỏ, mọng hình cầu, dùng lấy Tổng quan -3- sợi Thảo (Amomum tsaoko Roxb.) Sa nhân (A villosum Lour.) hai loại dùng làm thuốc, khai thác nhiều để xuất khẩu, gặp nhiều rừng miền Bắc Việt Nam 1.1.4 Phân loại thực vật Hệ thống phân loại Giới (Kingdom) Thực vật (Plantae) Phân giới (Subkingdom) Tracheobionta Ngành (Division) Magnoliophyta Lớp (Class) Liliopsida Phân lớp (Subclass) Zingiberidae Bộ (Order) Zingiberales Họ (Family) Zingiberaceae Tại hội nghị chuyên đề lần III Zingiberaceae tổ chức Thái Lan từ ngày - 12 tháng năm 2002 , Dr W John Kress đề nghị cách phân loại họ Gừng mới, dựa nghiên cứu gần đây, bao gồm phân tích đặc điểm phân tử hình thái Ông đề nghị chia họ Gừng thành phân họ lớn, gồm tông 53 chi, thể Bảng 1.2 Bảng 1.2 Phân loại họ Gừng theo W John Kress [23] Phân họ Phân họ Siphonochiloideae Tamijioideae Phân họ Alpinioideae Phân họ Zingiberoideae Tông Tông Tông Tông Tông Tông Siphonochileae Tamijieae Alpinieae Riedelieae Zingibereae Globbeae Siphonochilus Tamijia *Aulotandra Burbidgea Curcuma Aframomum Pleuranthodium Smithatris Mantisia Renealmia Riedelia Hitchenia Gagnepainia *Elettaria Siamanthus Stahlianthus Hemiorchis *Leptosolena *Paracautleya *Geostachys *Laosanthus *Geocharis Globba Camptandra Tổng quan -4- *Cyphostigma Pyrgophyllum Amomum Hedychium Paramomum Rhynchanthus *Elettariopsis Pommereschea Alpinia *Stadiochilus Plagiostachys *Nanochilus Vanoverberghia Cautleya Etlingera Roscoea Hornstedtia Haniffia Siliquamomum (Incertae Sedis) Boesenbergia Curcumorpha Kaempferia *Haplochorema Zingiber Distichochlamys Scaphochlamys Cornukaempferia *Parakaempferia Caulokaempferia (Incertae Sedis) * chi chưa có mẫu phân tích phát sinh lồi dựa đặc tính phân tử Theo nghiên cứu nước gần đây, họ Gừng Việt Nam có 19 chi với khoảng 136 - 145 loài [2] Sau chi thuộc họ Gừng (Zingiberaceae) có Việt Nam [2]: Chi Alpinia Roxb – Riềng, Sẹ Chi Amomum Roxb nom cons – Sa nhân, thảo Chi Boesenbergia Kuntze – Bồng nga truật Chi Caulokaempferia K Larsen – Đại bao khương Chi Cauley (Benth.) Royle ex Hook f – Cầu ly Chi Curcuma L nom cons – Nghệ Chi Distichochlamys M F Newman – Gừng đen Chi Elettaria (L.) Maton – Trúc sa, tiểu đậu khấu Tổng quan - 59 - Phụ lục B Hình PCR-RAPD với mồi Hình 1PLB Sản phẩm RAPD-PCR với mồi OPN 09 Hình 2PLB Sản phẩm RAPD-PCR với mồi OPP 08 Hình 3PLB Sản phẩm RAPD-PCR với mồi OPX 01 Hình 4PLB Sản phẩm RAPD-PCR với mồi OPX 04 Phụ lục C - 60 - Hình 5PLB Sản phẩm RAPD-PCR với mồi OPX 14 Hình 6PLB Sản phẩm RAPD-PCR với mồi OPX 15 Hình 7PLB Sản phẩm RAPD-PCR với mồi OPZ 03 Hình 8PLB Sản phẩm RAPD-PCR với mồi OPE 07 Kí hiệu mẫu: Giếng → giếng 13: N1 → N13; Giếng 14→ giếng 25: N18 → N29; M: thang 100 bp Phụ lục C - 61 - Phụ lục C Trình tự ITS mẫu thực nghiệm PLC1 Kết đọc peak ITS N12, mồi ITS 5P BIO TRACE Model 3100 File: 12DF_2010-02-05_11-58-23_G11_AN.ab1 KB.bcp 6286250-02 6286202-01 6286103-04 6258005-00 12DF Lane 13 Signal G:1446 A:909 T:1116 C:968 KB_3130_POP7_BDTv3.mob ?? no 'MTXF' field Points 864 to 12229 Page of 2/5/2010 Spacing: 12.3548269271851 BioEdit v ersion 7.0.5.3 (10/28/05) C T A T G A T TA C G TG AG AG C G TG AACA T T G T T G AG AG T G CA T TG AAT G AT G G AT G G T T G C G AAT G T G T CAA CG T G C CC C T T T C C T T G C C C C A T G T T G C T G G G C AA T T G AT C G T A G C 10 20 30 40 50 60 70 80 90 100 110 T C G G TG C G AT CG G C A C C A AG G A AT A AT A A A C T G AG A AGC A A AG G G C C C T CG G TG T G T G CG GG G AG C C C AA TG C G T C G G AG AAG C C T C G A AAT CA A AT G AC T C T C G G C A AT G G AT A T C T C G 120 130 140 150 160 170 180 190 200 210 220 230 G C T C T T G CAT CG AT G AAG A AC G T AG T G A AAT G C G A T A C T T G G T G T G A AT T GC AG A AT C T C G T G AA T CA T T G AG T C T T TG A A CG CA AG T TG T G C C C G AG G C C T TG T G GC CG AGG G C ACG C C T G C 240 250 260 270 280 290 300 310 320 330 340 350 T T GG GC G T C AT G G CA T CG T CG C C T T TG C T C CT T G C T T T GC T G C T G G T G C T A AG T G C GG A AAT T G G C C T CG T G T G CC C T CG G G C G AGG GC ACA G T C G G T T G A A G A G T G GG T A G T C G G T AG A CG 360 370 380 390 400 410 420 430 440 450 460 470 T CG G G C G CG A T G G G T G T T G G T C A C T C T AT G C G T G A AT C G A AC AT CG T C CC C G T C G T A C T G G G AT G A G T C C T C A AG AG AC C T T G T G T G AT T G C AG C AT C G C A T G A A AG T G C C G T G T T C A 480 490 500 510 520 530 540 550 560 570 580 590 PLC2 trình tự ITS mẫu thực nghiệm >N1 actctcggcaatggatatctcggctcttgcatcgatgaagaacgtagtgaaatgcgatacttggtgtgaattgcagaatcgcctcaaccattgattc tttgaacgcaggttgtgacgcaggccgtgtggtggagggcccgcctgcttgtcgggcctgacgtcctcttttttgctccttgcttcgtcggcattga gcacggaggtcggccgcgcgcgccctctggcacagtcggtcaacgagtgggtagtcggtaatcagcaagcacgatggacgttggtcgtcgc gagcaataactgaacgtcttgtcctcgtcgttttgggatgagtcctccagagaccctgtgtgatgattgcggagtcgcgtgaaagcgccgcgtca atcatttgcggccccaagtcaggcgagaccacccgccgagtttaagcatataaataagcggaggaggagaaacttacgaggattcccctagta acggcgagtgaaccgggatcagcccagcttgagaattgagcggatcc >N2 tcacaaggtttccttagggaactgcggaggtcattgttgagagagcatagcatagaatgatggatgattgcgaacgtgtgaacgtgaccctttcgt ctgcccctcccatgttggtgggcgattgaccgtagctcggtgcgatcggcactaaggaacaacgaacttggaagcagaggacccccttagcgt gagcggggagcccaatgcgtcggagattcttcggaatcaaatgactctcggcaatggatatctcggctcttgcatcgatgaagaacgtagtgaa atgcgatacttggtgtgaattgcagaatctcgtgaaccattgagtctttgaacgcaagttgtgcccgaggccttgtggtcgagggcacgcctgctt gggtgtcatgacatcgtcgcttttgctccatgcttcgtcggcattgagcgcggaagttggccccgtgtgccctcgggcacagtcggtcgaagag tgggtagtcggtaatcgtcgagcacgatggacgttggtcgtcgcgagcgagaactgaacgtcgtgtcctcgtcgttttgggatgagtcctccag agaccctgtgtgatgattgcggagtcgcgtgaaagcgccgcgtcaatcatttgcggccccaagtcaggcgagaccacccgccgagtttaagc atataaataagcggaggaggagaaacttacgaggattcccctagtaacggcgaggaaccgggtcagcccagctgagattacgtacta >N3 Phụ lục C - 62 - ctagctactgcggaggacattgttgagagagcatagcatagaatgatggatgattgcgaacgtgtgaacgtgaccctttcgtcgtcccatcccat gttggtgggcgattgaccgtagctcggtgcgatcggcactaaggaacaacgaacttggaagcagaggacccccttagcgtgagcggggagc ccaatgcgtcggagattcttcggaatcaaatgactctcggcaatggatatctcggctcttgcatcgatgaagaacgtagtgaaatgcgatacttgg tgtgaattgcagaatctcgtgaaccattgagtctttgaacgcaagttgtgcccgaggccttgtggtcgagggcacgcctgcttgggtgtcatgac atcgtcgcttttgctccatgcttcgtcggcattgagcgcggaagttggccccgtgtgccctcgggcacagtcggtcgaagagtgggtagtcggt aatcgtcgagcacgatggacgttggtcgtcgcgagcgagaactgaacgttgtgtcctcgtcgttttgggatgagtcctccagagaccctgtgtga tgattgcggagtcgcgtgaaagcgccgcgtcaatcatttgcggccccaagtcaggcgagaccacccgccgagtttaagcatataaataagcg gaggaggagaaacttacgaggattcccctagtaacggcgaggaaccgggtcagcccagctgagattacgtacga >N4 tgcggaggtcttgttgagagagcaataacagaatgatggatggttgcgaaacgtgtgaatgtgtccctttcctttgcccctcgacgtgcaagcatg cgtgcatgcatgtcggcgggcgattaacgatagctcgtcgctatcatcactactgaagaatgatctcctaagcaacggactcctcgtcgtgctca gcgagcccaatgcttcatagaatcctcgtaatcaatcatcatgactctcgactatggatatctcagctcttgcatctatcatcaatgaagtgaattgc gatacgtgatgtgtgttgtgaaatctcgagtatcattaactctttgatcgctagttgtgcctgatgccttctggcttatggcacacctgcttgctagtca tgagtttgccgcctcccctccacgctatgctggtgcttagcgcgtagatcgtccacgtgtgccctctggcatcgtcggccgacgaacgagcagt cgccagtcgtcggtcacgatggatgatggtcgctgatatcagcgataacagaaaatcgtccctctcctctctcgactaacgatcaaccgacccta tgtgatcgatgattctggcgtcagtgcctcgtcccgtccatcgatcatctagttgtcgccacgcgtgagagaccacccaccgaccacattcatcta attaatcgaaggaagaaaaaataactacgatccttctactaataacgaacaaacaaccatcatcc >N5 gggaaggagaagtcgtaacaaggtttccgtaggtgaacctgcggaggatcattgttgagagagcatatagaatatgacggatggctgcgaac gtgtgaatgtgcccctttcctcgccsccacccacccacacccgtgtgtgtcggttggcgggcggttgaccgtagctcggtgcgatcggcactaa ggaacaaatgaactcggaagcggagggccccttggcgtgcgcaggggagcccaacgcgtcggagattcctcggaatcaaacgactctcgg caatggatatctcggctcttgcatcgatgaagaacgtagtgaaatgcgatacttggtgtgaattgcagaatctcgtgaaccattgagtctttgaacg caagttgtgcccgaggccttgtggccgagggcacgcctgcttgggcgtcatggcatcgtcgcctcagccccattccctgttgtcagtcggctgg tgctgagcgcggaaattggccccgtgtgccctcgggcacagtcggccgaagagcgggtagtcggtaagtcgtcgggcacgacgggcgttg gtcgccgtgagcgggaacagaacgtcgtccccgtcattttcggacgagccctcaagagaccctgcgtggctgcggcaccgggcgaacgaaa gaacgcgccgtgtccatcaacttgtggccccaagtcaggcgagaccacccgccgaatttaagcatataaataagcggaggaggagaaactta cgaggattcccctagtaacggcgagtgaaccgggatcagcccagctc >N6 ggcctctgggaaggagaagtcgtaacaaggtttccgtaggtgactgcggaggatcattgttgagagagcatatagacggatgactgcgaatgt gtgaatgtgtcctttcctcgcccacaccccccatgtgtcggtggcgggcgattgaccgtagctcggtgcgatcggcactaaggaacaaatgaac tcggaagcggagggcccccttggcgtgcacaggggagcccaatgcgtcggarattcctcggaatcaaacgactctcggcaatggatatctcg gctcttgcatcgatgaagaacgtagtgaaatgcgatacttggtgtgaattgcagaatctcgtgaaccattgagtctttgaacgcaagttgtgcccg aggccttgtggccgagggcacgcctgcttgggtgtcatggcatcgtcgcctctgctccatgcgccgttatagtcggttggtggtgccgagcgcg gaaattggccccgtgtgccctcgggcacagtcggccgaagagcgggtagtcggcagtcgtcgggcacgatgggtgttggtcgccgtgagcg ggaacagaacgtcgtccccgtcgttttcggacgatccctcaagagaccctgcgtgattgtgtggcaccgggcgaaagaaagaaagcgccgtg tccaccatcaacttgtggccccaagtcaggcgaggccacccgccgaatttaagcatataaataagcggaggaggagaaacttacgaggattcc cctagtaacggcgagtgaaccgggatcagcccagctgagaattgagcg >N7 ctagcgacgtgcggaggatcattgttgagagagcatcatagaatgatggaggattgtgaacgtgtgaacgtgaccctttcgtcgtcagcccgcc catgttggtgggcgattgaccgtagctcggtgcgatcggcactaaggaacaacgaacttggaagcagagggcccctcagcgtgagcgggga gcccaatgcgtcggagattcttcggaatcaaatgactctcggcaatggatatctcggctcttgcatcgatgaagaacgtagtgaaatgcgatactt ggtgtgaattgcagaatctcgtgaaccattgagtctttgaacgcaagttgtgcccgaggccttgtggtcgagggcacgcctgcttgggcgtcatg acattgtcgcttttgctccatgctttgtcggcattgagcgcggaagttggccccgtgtgccctctcgggcacagtcggtcgaagagcgggtagtc ggtaatcgtcgagcacgatggacgttggtcgtcgcgagcgagaactgaacgtcgtcctctccgttttgtgatgagacctctccagaccctgtgtg attgatgattgcggactcgcgagaaagcgccccgtcaatcatttgcggccccaagtcaggcgagaccacccgccgagtttaagcatataaata agcggaggaggagaaacttacgaggattcccctagtaacggcgagtgaaccgggatcagcccagctgagaat >N8 ggtgggactggggaggacattgttgagagagcatcatagaatgatggatgattgtgaacgtgtgaacgtgaccctttcgtcggcccgcccccc atgtgggggggggattgaccgtagctcggtgcgatcggcagtaaggaacaacgaactaggaagcagagggcccctcagcgtgagcgggg agcccaatgcgtcggagattcttcggaatcaaatgactctcggcaatggatatctcggctcttgcatcgatgaagaacgtagtgaaatgcgatac ttggtgtgaattgcagaatctcgtgaaccattgagtctttgaacgcaagttgtgcccgaggccttgtggtcgagggcacgcctgcttgggcgtcat Phụ lục C - 63 - grcatygtcgcttttgctccatgctttgtcggcattgagcgcggaagttggccccgtgtgccctcgggcacagtcggtcgaagagcgagtagtc ggtaatcgtccagcaagatggacgttggtcgtcccgagcgagaactgaccgtcgtcctcgtcgttttgggatgagcgccatcgagagcccgtg tgtgatgagtggggccgcgcgagaaagccccgcgtcaatcatttgcggccccaagtcaggcgagaccacccgccgagtttaagcatataaat aagcggaggaggagaaacttacgaggattcccctagtaacggcgagtgaaccgggatcagcccagctta >N9 atttgagatggaaggagaaatcttacaaggttaccgtaggtgaacgtgcggaaggatcattgttgagagagcatagaatgaatgatggatgata gagaacgagcgaacgtcgtccctttgtcccgtccccccaacttggtgggcgattgaccgtagctcggtgcgatcggcgctaaggaacaacga acttgtaggcagaagggccccctcagcgtggagcggggagccccgtgcgtcggagagtttcttcggaatcaaatgactctccgcaatggatat ctcggctcttgcatcgatgaagaaaatagtgaaatgcgatacttggtgagaattgtagaatctcgtgaaacattgagtctttgaacgcaagttgtgc ccgaggccttgtggtcgagggcacacccgcttgggggtcatgatatcgtcgctttttctccctgctttgtcggcatttagcgcggaaattggcccc ctgtgcccccgggcacagtcggtcgaagagtgggtagtcggtaatcgtccagcacgatggacgttggtcgtcgcgagcgagaactgaacgtc gtcctcgtcgttttgggatgagtcctcmataagagaccctgtgtgatgattgcggactcgcgcgaaagcgccccgtcaatcattt >N10 tcttgtgatgcggaggtcttgttgagagagcatcatagaatgatggatgattgtgaacgtgtgaacgtgaccctttcgtcatcagcccgcccatgtt ggtgggcgattgaccgtagctcggtgcgatcggcactaaggaacaacgaacttggaagcagagggcccctcagcgtgagcggggagccca atgcgtcggagattcttcggaatcaaatgactctcggcaatggatatctcggctcttgcatcgatgaagaacgtagtgaaatgcgatacttggtgt gaattgcagaatctcgtgaaccattgagtctttgaacgcaagttgtgcccgaggccttgtggtcgagggcacgcctgcttgggcgtcatggcatc gtcgcttttgctccatgctttgtcggcattgagcgcggaagttggccccgtgtgccctctcgggcacagtcggtcgaagagcgggtagtcggta atcgtcgagcacgatggacgttggtcgtcgcgagcgagaactgaacgtcgtcctcgtcgttttgggatgagccctcaataaagagaccctgtgt gattgatgattgcggactcgcgcgaaagcgccgcgtcaatcatttgcgtgccgacgtcaggcaagacagaccacccagctagatcatacatat aagtggacgaagaaagacatactaagaatcactcacttactaccagtgaatgaggctcgatccgcctagcttgttagactgatgaatg >N11 gaaacttgattgtggacggagaccatcctttgcgggtgagcccggaagcccaatggattggagattcttcggaatcaaatgactctgggcaatg gatatctcggctcttgcatcaatgaagaaagtagtgaaatgcgatacttggtgtgaattgcagaatctcgtgaaccattgagtctttgaacgcaagt tgggcccggggccttgtggtcgagggcacgcctggttgggtgtcatgacattgtcgcttttgcttcatggttagtgggcattgagcgcggaagtt ggccccgtgtgccctcgggcacagtcggtcgaagagtgggtagtcggtaatcgtcgagcacgatggacgttggtagtcgcgagcgagaattg aatgttggttcctcgtcgttttgggatgagtcctccagagaccctgtgtgatgattgcggagtcgcgtgaaagcgccgcgtcaatcatttgcggcc ccaagtcaggcgagaccacccgccgagtttaagcatataaataagcggaggaggagaaacttacgaggattcccctagtaacggcgagtga accgggatcagcccagctgagaa >N12 aggtttccgtaggtgaacctgcggaaggatcattgttgagagtgcattgaatgatggatggttgcgaatgtgtcaacgtgcccctttccttgcccc atgttgctgggcaattgatcgtagctcggtgcgatcggcaccaaggaataataaactgagaagcaaagggccctcggtgtgtgcggggagcc caatgcgtcggagaagcctcgaaatcaaatgactctcggcaatggatatctcggctcttgcatcgatgaagaacgtagtgaaatgcgatacttgg tgtgaattgcagaatctcgtgaatcattgagtctttgaacgcaagttgtgcccgaggccttgtggccgagggcacgcctgcttgggcgtcatggc atcgtcgcctttgctccttgctttgctgctggtgctaagtgcggaaattggcctcgtgtgccctcgggcgagggcacagtcggttgaagagtggg tagtcggtagacgtcgggcgcgatgggtgttggtcactctatgcgtgaatcgaacatcgtccccgtcgtactgggatgagtcctcaagagacctt gtgtgattgcagcatcgcatgaaagtgccgtgttcatcatattgtggccccaagtcaggcgaggcccacccgccgagtttaagcatataaataag cggaggaggagaaacttacgaggattcccctagtaacggcgagtgaatcgggatcagcccagcttgagaatttgacggttgaatccgtccga gtt >N13 cgttgctactgcggaggacattgttgagagagcatatagacggatgactgcgaatgtgtgaatgtgtccctttcctcgcccacaccccccatgtgt cggtggcgggcgattgaccgtagctcggtgcgatcggcactaaggaacaaatgaactcggaagcggagggcccccttggcgtgcacaggg gagcccaatgcgtcggagattcctcggaatcaaacgactctcggcaatggatatctcggctcttgcatcgatgaagaacgtagtgaaatgcgat acttggtgtgaattgcagaatctcgtgaaccattgagtctttgaacgcaagttgtgcccgaggccttgtggccgagggcacgcctgcttgggtgt catggcatcgtcgcctcttctccatgccctgttagacagtcggctggtggtgttgagcgcggaaattggccccgtgtgccctcgggcacagtcg gccgaagagcgggtagtcggcagtcgtcgggcacgatgggtgttggtcgccgtgagcgggaacagaacgtcgtccccgtcgttttcggacg agccctcaagagaccctgcgtgattgtggcaccgggcgaaagaaagaaagcgccgtgtccaccatcaacttgtggccccaagtcaggcgag gccacccgccgaatttaagcatataaataagcggaggaggagaaacttacgaggattcccctagtaacggcgagtgaaccgggatcagccca gcttgagaattgagcgg >N18 Phụ lục C - 64 - cgagctactgcggaggacattgttgagagagcatatagacggatgactgcgaatgtgtgaatgtgtccctttcctcgcccacaccccccatgtgt cggtggcgggggattgaccgtagctcggtgcgatcggcactaaggaacaaatgaactctgaagcggagggcccccttggcgtgcacaggg gagcccaatgcgtcggagaatcctccgaatcaaacgactctcggcaatggatatctcggctcttgcatcgatgaagaacgtagtgaaatgcgat acttggtgtgaattgcagaatctcctgaaccattgagtctttgaacgcaagttgtgcccgagaccttgtggccaagggcacccctgcttgggtgtc atggcatcgtcgcctcttctccatgccccgttagacagtcggctggtggtgctgagcgcggaaattggccccgtgtgccctcgggcacagtcg gccgaagagcgggtagtcggcagtcgtcgggcacgatgggtgttggtcgccgtgagcgggaacagaacgtcgtccccgtcgttttcggacg agccctcaagagaccctgcgtgattgtggcaccgggcgaaagaaagaaagcgccgtgtccaccatcaacttgtggccccaagtcaggcgag gccacccgccgaatttaagcatataaataagcggaggaggagaaacttacgaggattcccctagtaacggcgaggaaccgggtcagcccag c >N19 ctagctactgcggaggatcattgttgagagagcataacgaatgatggatggttgcgaatgtgtgaatgtgaccctttcctcgccccacccaccca cgtcggtggggggtcgatcgaccgtagctcggtgcgatcagcactaaggaacaatgaactcagaagcggagggccccttggggtgcgcgg gggagcccaatgcgtcggagattcctcggaatcaatcaaatgactctcggcaatggatatctcggctcttgcatcgatgaagaacgtagtgaaat gcgatacttggtgtgaattgcagaatctcgtgaaccattgagtctttgaacgcaagttgtgcccgaggccttgtggccgagggcacgcctgcttg ggtgtcatggcatcgccgcctctgctccatgctttgttcgtgggtgccgagcgcggaaattggccccgtgtgccctcgggggcacagtcggct gaagagcgggtagtcgccagtcgtcgggcacgatgggtgttggtcgccgtgagcgggaacagaacgtcgtgtccccgtcgttttgggatgat gagccctcagtcaagagaccctgtgtgatggtttgtgcctataaacaatcaacttgtggccccaagtcaggcgaggccacccgccgagtttaag catataaataagcggaggaggagaaacttacgaggattcccctagtaacggcgagtgaaccgggatcagcccagcttgagaat >N20 gaccgtagctcggtgcgatcggcactaaggaacaatgaacttgaaagcagagggccctttggcgtgagcggggagcacaatagcgtcaaag attcttcggaatcaaatgactctcggcaatggatatctcggctcttgcatcgatgaagaccgtagtgaaatgcgattcttggtgtgaattgcagaat ctcgcgaaccattgagtctttgaacgcaacaagttccccagtccatgtggtcgagggcacgcccatttgggtgtcatgacatcgtcgcttatgctc catgccttgttggcatagagttcggaaattgcccccgtgtaccgtcgggcacattcggtagaagagtgggctctcggcaattgtcgagctcgatg gggtttgttagtcgcacaagagacgagatagtcgtcctcgtcattcatggatgagtcctcaagaggccctatgtatgtgattgcagagtcggctca aagcgctgtgtcaatcatcatattgggcggccgccactcaggcgagaccacccgccgattttacgcatataaataactggaggagaggaacttt cgaggattcccctagtaccggcgagtgatccgggtttagcccagatccagaagaa >N21 gttggtgggcattgactagagctcggtgcgatccgcactaaggaacaataaaatcggaagcagagggccccttggcatgcacagggagccc aatgcgtcggagattcctcggaatcaaatgactctcggcaatggatatctcggctcttgcatcgatgaagaacgtagtgaaatgcgatacttggtg tgaattgcagaatctcgtgaaccattgagtctttgaacgcaagttgtgcccgaggccttctggtcgagggcacgcctgcttgggcgccatggcat cgtcgcctttgctccaagcgttgttggtgctgagtgcgaaaattgaccccgtgtgccctcgggcatagtcggttgaagagggggtagtcggcag tcgtcgggcacgatgggtgttggtcgccgtgagcgggaacagaacatcggcctcgtcatttttggacgagtcctcaagagaccctgttagattgt ggcatcaggtcaaagtgccaagtccaccaacttgtggccccaagtcaggcgaggccacccgccgagtttaagcatataaataagcggagga gaagaaacttacaaggattcccctagtaacggcgagtgaaccgggatcagcccagctgagaatttgacggttgaaaccgttcgagttgtagtct gggaagc >N22 aatgcgcccctttccttgccccccatgtcggcgggcattgaccgtagctcggtgcgatccgcactaaggaacaatgaactcggaagcagacgg gccccccggcgtgcgcaagggagcccgatgcgtcggagattcttcggaatcaatcaaatgactctcggcaatggatatctcggctcttgcatcg atgaagaacgtagtgaaatgcgatacttggtgtgaattgcagaatctcgtgaaccattgagtctttgaacgcaagttgtgcccgaggccttgtggc cgagggcacgcctgcttgggcgtcatggcaccgtcgccttcgctccatgcgtggttggcgcgagcgcggaaattggccccgtgtgccctagg gcacagtcggtcgaagagcgggcagtcggcaatcgtcgggcacgatgggcgttggtcgccgtgagcgggaacagaacatcgtcctcgtcgt tttgggacgagccctagatagagaccctgtgcgattgcggcatcggacgaaagcgccgtgcccatccatctacttgtggccccaagtcaggcg aggccacccgccgagtttaagcatataaataagcggaggaggagaaacttacgaggattcccctagtaacggcgagtgaaccgggacaagc ccagcttgagaatttaacggttgaatccgttcgagttgtagctggggaagc >N23 ggtgaataatgtagtgaaatgcgatacttggtgtgaattgcagaatctcgcgaaccattgagtctttgaacgcaagttgtgcccgaggccttgtgg tcgagggcacgcctgcttgggtgtcatggcatcgtcgcttttgctccatgcttttttggcattgagcgcggaaattggccccgtgtgccctcgagc acagtcggtcgaagagtgggtagtcggtactcgtcgagcacgacggacgttggtcgccatgagcgggaactgaacgtcgtcgctcgtcgtttt gggaatagtcctcaagagaccctgtgtgatgatwgcggagtcggatgaaagtgccgtgtcaatcatttgtggccccaagtcaggcgagacca cccgccgagtttaagcatataaataagcggaggaggagaaacttacgaggattcccctagtaacggcgagtgaaccgggtcagcccagctga gaatg Phụ lục C - 65 - >N24 aacctacggaaggatcattgttgagagagcatcgaatgacggatgtttgtgaacgtgtgaatgtgttcctttcctcgccccccaatgcctttggcg ggcgattgactgtaactcggtgcgatcggcactaaggaacaatgaactcggaagcggagggccccttgccgtgcgcagggagcccaacgc gtcagagattcctcggaatcaaatgactctcggcaatggatatctcggctcttgcatcgatgaagaacgtagtgaaatgcgatacttggtgtgaat tgcagaatctcgtgaaccattgagtctttgaacgcaagttgtgcccgaggccttctggccgagggcacgcctgcttgggagtcatggcaccgcc gcctctgctccatgcaatattggtgcaccagagcgcgtaaagtggccccgtgtgccctcgggcacagtcggctgaagagcgggtagtcatcaa tcgtcgggcacgacgggtgttggtcgccgcgatgcgggaacagaacgtcgcccccgtcggttcgggacgagccctcaaagagaccctatgt gattgtggcgtcgggtgaaagtgccgcgtccatcaacttgtggccccaagtcaggcgagaccacccgctgagtttaagcatataaataagcgg aggaggagaaacttacgaggattcccctagtaacggcgaggaaccgggtcagcccagctgagattacgtacaga >N25 gatactgggtgtgaattgcagaatctcgtgaaccattgagtctttgaacgcaagttgtgcccgaggccttgtggtcgagggcacgcctgcttggg cgtcatgacattgtcgcttttgctccatgctttgtcggcattgagcgcggaagttggccccgtgtgccctctcgggcacagtcggtcgaagagcg ggtagtccgtaatcgtcgagcacgatggacgttggtcgtcccgagcgagaactgaacgtcctcctcgtcgttttgggatgagccctcactaaga gagcccgtgtgatgaagatgtggagccgcgcgagaagccccgcgtcaatcatttgcggccccaaagtcaggcgagaccacccgccgagttt aagcatataaataagcggaggagaagaaacttacgaggattcccctagtaacggcgagtgaaccgggatcagcccagctgagaattgaacg gattgagttttattctgtggaagtgtgagagagcgtgaccagagataaaaaaaactgactttcctttgttttatgagaaaccgccacccaccccctc gcatagtcgctgtc >N26 aagctagtgaaaagagaaacttggtgtgaattgcagaatctcgtgaaccattgagtctttgaacgcaagttgtgcccgaggccttctggccgagg gcacgcctgcttgggagtcatggcattgccgcctttgcaccaccacgatgtgttggtgctgagcgcgtgaaattggccctcgggtgccctcggg caccgtccgttgaagagtgggcagtcgccaatcgtcgggcaccatgggtgttggtcgcggtgagcggtgaacagaacagtcgtccccgtcgt agttgggatgaagtcccctgacaccctgagtgattgcggcgtcgcggaaaaggggccatgtccatcaacttgtggccccaagtcaggcgagg cccacccgccgagtttaagcatataaataagcggaggaggagaaacttacgaggattcccctagtaacggcgagtgaaccgggatcagccca gcttgagaatttgagcggattcttttcgagttatatgtgcgagaggttgactggagaaagcgtgaaataaccattatcctttgcccaaagactttcaa ttggtccggaagacccccctgactcc >N27 cctttggtatatctatttttgcacttatgaagaaaataaagaaataggaacttgggtgtgaattgcagaatctcgtgaaccattgagtctttgaacgca agttgtgcccgaggccttgtggtcgagggcacgcctgcttgggcgtcatggcattgtcgcttttgctccatgctttgtcggcattgagcgcggaa gttggccccgtgtgccctcgggcacagtcggtcgaagagcgagtagtcggtcatcgtcgagcacgatggacgttggtcgtcgcgagcgaga actgaacgtcctcctcctccttttgggatgagtcatccagaagaccctgtgtgattgatgattgcggacccgcgcgaaagcgccgcgtcaatcat ttgcggcgcccaagtcgagcgcgagaccacccgccgagtttaagcatataaataagcggaggaggagaaacttacgaggattcccctagtaa cggcgagtgaaccgggatcagcccagctgagaatgatcgggaatgttttagtctggtgtggtggagagtgagaaagcgtgagcgagaactga ctgtccttctccctctt >N28 tgcggaggatcattgttgagagagcataataaagtgatggatggttgtgaacgtgtgaatgtgtcttaccttacccctatgtacccatccatctgac cgtagctcggtgcggccttaaggacacaataagaaatttggaagcaggggcccccgacgtgcgcggagagccccccgtctcagagatttttc agaaataaatgactcttcacagcaaatatcttttctcttgtagagagaagaagaaagaaataaaatacttggtgtgaagaagagaatcttgtgaca caccgtgtttttgtgcaagacgtgcgagaggctgtgtggccgggggcacgcctgcttgggacgtcatggcatcgtcgcctcttctcctcgatgtg atggtggtgagcgtgaaaaaactccccgcgccctcccctcctcgtggggccaccacagtcggtagaggggggggtcgtctcatgtcgtacgc caggagtgttggtcgtcgccaggggcgggaacatatcatcgtcctcgttgtggtgggcccacccttcaaggaagaaagaagaccctgtgtgat ttgactgcggcggcgggcgaaagtgccacgtcgtccgtccatcaatcaacttgtggccccaagtcaggcgagaccacccgccgagtttaagc atataaataagcggaggaggagaaacttacgaggattcccctagtaacggcgagtgaaccgggatcagcccagcttgagaatt >N29 gacggatggctgcgaacgtgtgaatgtgcccctttcctcgcccccacccacccacacccgtgtgtgtcggttggcgggcggttgaccgtagct cggtgcgatcggcactaaggaacaaatgaactcggaagcggagggccccttggcgtgcgcaggggagcccaacgcgtcggagattcctcg gaatcaaacgactctcggcaatggatatctcggctcttgcatcgatgaagaacgtagtgaaatgcgatacttggtgtgaattgcagaatctcgtga accattgagtctttgaacgcaagttgtgcccgaggccttgtggccgagggcacgcctgcttgggcgtcatggcatcgtcgcctcagccccattc cctgttgtcagtcggctggtgctgagcgcggaaattggccccgtgtgccctcgggcacagtcggccgaagagcgggtagtcggtaagtcgtc gggcacgacgggcgttggtcgccgtgagcgggaacagaacgtcgtccccgtcattttcggacgagccctcaagagaccctgcgtgactgcg gcaccgggcgaacgaaagaacgcgccgtgtccatcaacttgtggccccaagtcaggcgagaccacccgccgaatttaagcatataaataag cggaggagggaaacttacgaggattcccctagtaacggcgagtgaaccgggatcagcccagcttgagaattgaacgggattagcc Phụ lục C - 66 - Phụ lục D Trình tự trnK - matK mẫu thực nghiệm PLD1 Kết đọc peak trnK N12, mồi mIF BIO TRACE Model 3100 File: 28TH1_2010-08-31_20100831.ab1 KB.bcp ðÍ-D' 6286250-02 6286202-01 6286103-04 6258005-00 28TH1 Lane Signal G:73 A:85 T:83 C:56 KB_3130_POP7_BDTv3.mob ?? no 'MTXF' field Points 956 to 12314 Page of 8/31/2010 Spacing: 12.4696292877197 BioEdit version 7.0.5.3 (10/28/05) AAGC A AGA A TTA TTAT T T G AT TTAT T CG G T T AT G ATAT T T AC CA A A AT A T AT T T G T T G G G CAT A ACA C CT T T T T TA A T T T T CA T T T T T T T T C TC AG A T T C TA T C T G AG G G T T T T GC AG T C 10 20 30 40 50 60 70 80 90 100 110 120 A T TG T A A AA AC T C C A T T T T CGC T G C AG T T AA T A T C T T C C C T TG AAG A A A A AG AA AT A CC A A A AT C T CA C A A T T TA CAA T C T A G T CA T T C A A T AT T T C C T T T T T TAG AG G AT AA AT T G T 130 140 150 160 170 180 190 200 210 220 230 T GC A T T TA A A T T A T C T G TC C G AT A TAC T A A T AC C C T AT C C C G T C CA T A T G G AA A TC T T G G T CC A A AT G C T T CA A T C C TG G A T C C AG G AT G T TC T C T C T T T A C AT T T AT T G C AG T TC C T T 240 250 260 270 280 290 300 310 320 330 340 350 C T CC ACG A AT AT T A T AA T T G G A A T AG T C T C A T T AT T CC G AA A AA ATC T AT T T AC G T AT T T T C A AA AG A AA AT A AA AG AT T AT T T T G G T T CT T A T AT A AT T T A T AT A T AT A T G A A T A C G A AT T 360 370 380 390 400 410 420 430 440 450 460 470 T C T A T T AG T G T T T CC T TG T A AA C AA T C C T C T T T T T T AC G A T T A AT AT C T T C T G G AG TC C T T C T T G AG CG A AT A C A T T T T T AT G T A A A AAT AG AA C AT C T T G G AG T G T G CC G A AT T T T T T 480 490 500 510 520 530 540 550 560 570 580 590 G T CA G A AG ACT C T AT G G AT T T T CA A GG AT C C T T T C AT AC A T T AT AT T C G A T AT C A AG G A A A A T C G A T T C T G GG T T C A AG A G G G AC T C A T T T T T T G AT G A AG A AAT GG AA AT AC C AC CT T 600 610 620 630 640 650 660 670 680 690 700 710 G T T A AT T T T T G G C AA T A T T AT T T T C AT T T T T G G T C T C AA CC A T A T A GG AT T G AT AT AA AG A A AT T AT C A A A C T A T T C T T T T T A T T T T C T G GG T T AT T T T T C A AG T G T AC A AA T T AA T T C T 720 730 740 750 760 770 780 790 800 810 820 830 T T G AT G G T AAG G A AT C A AA T G C T AG AG A A T T C AT T T C T A AT G G AT A C T CT T A CT A AG AA AT T T G AT AC T AT A AT C C C AA T T A T T C CT C T T AT T CG AT C AT TG T CT AA A G C T C A ATT T T GT A 840 850 860 870 880 890 900 910 920 930 940 950 Phụ lục C - 67 - PLD1 Kết trình tự matK mẫu thực nghiệm >N1 atggaagaattacaaggatatttagaagaagatagatctcggcaacaacagtttctatatccacttctctttcaagaatatatttacgtatttgcttatg atcatgggttaaatagttcaattttttatgaaccccaaaactccttgggttatgacaataaatttagttcagtacttgtgaaacgtttaattattcgaatgt atcaaaaaaattatttgatttattcggttaatgatatttaccaaaatttatttgttgggcataacaattatttttattttaattttttttctcagattctatctgaag gttttgcagtcattgtagaaattccattttcgctgcagttaatatcttcccttgaagaaaaagaaataccaaaatctcacaatttacaatctagtcattca atatttccttttttagaggataaattattgcatttaaattatctgtccgatatactaataccctatcccgtccatatggaaatcttggtccaaatgcttcaat cctggatccaggatgttctctctttacatttattgcagttccttctccacgaatattataattggactagtctcattattccgaaaaaatctatttacgtattt tcaaaagaaaataaaagactattttggttcttatataatttatatatatatgaatacgaatttctattagtgtttccttgtaaacaatcctcttttttacgatta atatcttctggagtccttcttgagcgaatacatttttatgtaaaaatagaacatcttggagtgtgccgaattttttgtcagaagactctatggattttcaa ggatcctttcatacattatattcgatatcaaggaaaatcgattctgggttcaagagggactcattttttgatgaagaaatggaaataccaccttgttaa tttttggcaatattattttcatttttggtctcaaccatataggattgatataaagaaattatcaaactattctttttattttctgggttatttttcaagtgtacaaa ttaattcttcgatggtaaggaatcaaatgctagagaattcatttctgatggatactcttactaagaaatttgataccataatcccaattattcctcttattc gatcattgtctaaagctcaattttgtaccgtatctgggtatcctattagtaaaccaatttggaccgatttagcggattgtgatattattaatagatttggtc ggatatgtagaaagctttctcactatcacagtggatcctcaaaaaaacagagtttgtatcgaatgaagtatatacttcgactttcatgtgccagaact ttggcccgtaaacataaaagttcagcacgcagttttttgcaaagattaagttcgggattattagaagaattctttacggaagaagaacaagttattttt ttgatctttccaaaaataatttatttttatttatatgtatcatatagagaacgtatttggtatttggatattatccgtatcaatgacttggtaaattgtttattag tcacaacataa >N3 atggaagaattacaaggatatttagaagaagatagatctcggcaacaacagtttctatatccacttctctttcaagaatatatttacgtatttgcttatg atcatgggttaaatagttcaattttttatgaaccccaaaactccttgggttatgacaataaatttagttcagtacttgtgaaacgtttaattattcgaatgt atcaaaaaaattatttgatttattcggttaatgatatttaccaaaatatatttgtcgggcataaaaaaaaaattaattttaattttttttctcagattctatctg atcgttttgcagtcattgtagaaattccattttcgctgcagttaatatcttcccttgaagaaaaagaaataccaaaatctcacaatttacaatctagtcat tcaatatttccttttttagaggataaattattgcatttaaattatctgtccgatatactaataccctatcccgtccatatggaaatcttggtccaaatgcttc aatcctggatccacgatgttctctctttacatttattgcagttccttctccacgaatattataattggaatactctcattattccgaaaaaatctatttacgt attttcaaaagaaaataaaagactattttggttcttatataatttatatatatatgaatacgaatttctattagtgtttccttgtaaacaatcctcttttttacg attaatatcttctggagtccttcttgagcgaatacatttttatgtaaaaatagaacatcttggagtgtgccgaattttttgtcagaagactctatggatttt caaggatcctttcatacattatattcgatatcaaggaaaatcgattctgggttcaagagggactcattttttgatgaagaaatggaaataccaccttgt taatttttggcaatattattttcatttttggtctcaaccatataggattgatataaagaaattatcaaactattctttttattttctgggttatttttcaagtgtac aaattaattcttcgatggtaaggaatcaaatgctagagaattcatttctgatggatactcttactaagaaatttgataccataatcccaattattcctctt attcgatcattgtctaaagctcaattttgtaccgtatctgggtatcctattagtaaaccaatttggaccgatttagcggattgtgatattattaatagattt ggtcggatatgtagaaagctttctcactatcacagtggatcctcaaaaaaacagagtttgtatcgaatgaagtatatacttcgactttcatgtgccag aactttggcccgtaaacataaaagttcagcacgcagttttttgcaaagattaagttcgggattattagaagaattctttacggaagaagaacaagtt atttttttgatctttccaaaaataatttctttttatttatatggatcatatagagaacgtatttggtatttggatattatccgtatcaatgacctggtaaattgtt tattagtcacgacataa >N4 atggaagaattacaaggatatttagaagaagatagatctcggcaacaacagtttctatatccacttctctttcaagaatatatttacgtatttgcttatg atcatgggttaaatagttcaattttttatgaaccccaaaactccttgggttatgacaataaatttagttcagtacttgtgaaacgtttaattattcgaatgt atcaaaaaaattatttgatttattcggttaatgatatttaccaaaatatatttgtcgggcataaaaaaaaaattaattttaattttttttctcagattctatctg aaggttttgcagtcattgtagaaactcaattttcgctgcagttaatatcttcccttgaagaaaaagaaataccaaaatctcacaatttacaatctagtc attcaatatttccttttttagaggataaattattgcatttaaattatctgtccgatatactaataccctatcccgtccatatggaaatcttggtccaaatgttt caatcctggatccaggatgttctctctttacatttattgcagttccttctccacgaatattataattggaatagtctcattattccgaaaaaatctatttac gtattttcaaaagaaaataaaagattattttggttcttatataatttatatatatatgaatacgaatttctattagtgtttccttgtaaacaatcctcttttttac gattaatatcttctggagtccttcttgagcgaatacatttttatgtaaaaatagaacatcttggagtgtgccgaattttttgtcagaagactctatggattt tcaaggatcctttcatacattatattcgatatcaaggaaaatcgattctgggttcaagagggactcattttttgatgaagaaatggaaataccaccttg ttaatttttggcaatattattttcatttttggtctcaaccatataggattgatataaagaaattatcaaactattctttttattttctgggttatttttcaagtgta caaattaattcttcgatggtaaggaatcaaatgctagagaattcatttctgatggatactcttactaagaaatttgataccataatcccaattattcctct tattcgatcattgtctaaagctcaattttgtaccgtatctgggtatcctattagtaaaccaatttggaccgatttagcggattgtgatattattaatagattt ggtcggatatgtagaaagctttctcactatcacagtggatcctcaaaaaaacagagtttgtatcgaatgaagtatatacttcgactttcatgtgccag aactttggcccgtaaacataaaagttcagcacgcagttttttgcaaagattaagttcgggattattagaagaattctttacggaagaagaacaagtt Phụ lục C - 68 - ctttttttgatctttccaaaaataatttctttttatttatacggatcatatagagaacgtatttggtatttggatattatccgtataaatgacctggtaaattgtt tattagtcacgacataa >N5 atggaagaattacaaggatatttagaagaagatagatctcggcaacaacagtttctatatccacttctctttcaagaatatatttacgtatttgcttatg atcatgggttaaatagttcaattttttatgaaccccaaaactccttgggttatgacaataaatttagttcagtacttgtgaaacgtttaattattcgaatgt atcaaaaaaattatttgatttattcggttaatgatatttaccaaaatatatttgtcgggcataacaattatttttattttaattttttttctcagattctatctgaa ggttttgcagtcattgtagaaattccattttcgctgcagttaatatcttcccttgaagaaaaagaaataccaaaatctcacaatttacaatctagtcattc aatatttccttttttagaggataaattattgcatttaaattatctgtccgatatactaataccctatcccgtccatatggaaatcttggtccaaatgcttcaa tcctggatccaggatgttctctctttacatttattgcagttctttctccacgaatattataattggaatagtctcattattccgaaaaaatctatttacgtatt ttcaaaagaaaataaaagactattttggttcttatataatttatatatatatgaatacgaatttctattagtgtttccttgtaaacaatcctcttttttacgatt aatatcttctggagtccttcttgagcgaatacatttttatgtaaaaatagaacatcttggagtgtgccgaattttttgtcagaagactctatggattttca aggatcctttcatacattatattcgatatcaaggaaaatcgattctgggttcaagagggactcattttttgatgaagaaatggaaataccaccttgtta atttttggcaatattattttaatttttggtctcaaccatataggattgatataaagaaattatcaaactattctttttattttctgggttatttttcaagtgtacaa attaattcttcgatggtaaggaatcaaatgctagagaattcatttctaatgtatactcttactaagaaatttgatactataatcccgattattcctcttattc gatcattgtctaaagctcaattttgtaccgtatctgggtatcctattagtaaaccaatttggaccgatttagcggattgtgatattattaatagatttggtc ggatatgtagaaagctttctcactatcacagtggatcctcaaaaaaacagagtttgtatcgaatgaagtatatacttcgactttcatgtgccagaact ttggcccgtaaacataaaagttcagcacgcagttttttgcaaagattaagttcgggattattagaagaattctttacggaagaagaacaagttattttt ttgatctttcctaaaataatttctttttatttatatggatcatatagagaacgtatttggtatttggatattatccgtatcaatgacctggtaaattgtttatta gtcacgacataa >N6 atggaagaattacaaggatatttagaagaagatagatctcggcaacaacagtttctatatccacttctctttcaagaatatatttacgtatttgcttatg atcatgggttaaatagttcaattttttatgaaccccaaaactccttgggttatgacaataaatttagttcagtacttgtgaaacgtttaattattcgaatgt atcaaaaaaattatttgatttattcggttaatgatatttaccaaaatatatttgtcgggcataacaattatttttattttaattttttttctcagattctatctgaa ggttttgcagtcattgtagaaattccattttcgctgcagttaatatcttcccttgaagaaaaagaaataccaaaatctcacaatttacaatctagtcattc aatatttccttttttagaggataaattattgcatttaaattatctgtccgatatactaataccctatcccgtccatatggaaatcttggtccaaatgcttcaa tcctggatccaggatgttctctctttacatttattgcagttccttctccacgaatattataattggaatagtctcattattccgaaaaaatctatttacgtatt ttcaaaagaaaataaaagactattttggttcttatataatttatatatatatgaatacgaatttctattagtgtttccttgtaaacaatcctcttttttacgatt aatatcttctggagtccttcttgagcgaatacatttttatgtaaaaatagaacattttggagtgtgccgaattttttgtcagaagactctatggattttcaa ggatcctttcatacattatattcgatatcaaggaaaatcgattctgggttcaagagggactcattttttgatgaagaaatggaaataccaccttgttaa tttttggcaatattattttcatttttggtctcaaccatataggattgatataaagaaattatcaaactattctttttattttctgggttatttttcaagtgtacaaa ttaattcttcgatggtaaggaatcaaatgctagagaatttatttctaatggatactcttactaagaaatttgatactataatcccgattattcctcttattcg atcattgtttaaagctcaattttgtaccgtatctgggtatcctattagtaaaccaatttggaccgatttagcggattgtgatattattaatagatttggtcg gatatgtagaaagctttctcactatcacagtggatcctcaaaaaaacagagtttgtatcgaatgaagtatatacttcgactttcatgtgccagaacttt ggcccgtaaacataaaagttcagcacgcagttttttgcaaagattaagttcgggattattagaagaattctttacggaagaagaacaagttattttttt gatctttccaaaaataatttctttttatttatatggatcatatagagaacgtatttggtatttggatattatccgtatcaatgacctggtaaattgtttattagt cacgacataa >N8 atggaagaattacaaggatatttagaagaagatagatctcggcaacaacagtttctatatccacttctctttcaagaatatatttacgtatttgcttatg atcatgggttaaatagttcaattttttatgaaccccaaaactccttgggttatgacaataaatttagttcagtacttgtgaaacgtttaattattcgaatgt atcaaaaaaattatttgatttattcggttaatgatatttaccaaaatttatttgttgggcataacaattatttttattttaattttttttctcagattctatctgaag gttttgcagtcattgtagaaattccattttcgctgcagttaatatcttcccttgaagaaaaagaaataccaaaatctcacaatttacaatctagtcattca atatttccttttttagaggataaattattgcatttaaattatctgtccgatatactaataccctatcccgtccatatggaaatcttggtccaaatgcttcaat cctggatccaggatgttctctctttacatttattgcagttccttctccacgaatattataattggactagtctcattattccgaaaaaatctatttacgtattt tcaaaagaaaataaaagactattttggttcttatataatttatatatatatgaatacgaatttctattagtgtttccttgtaaacaatcctcttttttacgatta atatcttctggagtccttcttgagcgaatacatttttatgtaaaaatagaacatcttggagtgtgccgaattttttgtcagaagactctatggattttcaa ggatcctttcatacattatattcgatatcaaggaaaatcgattctgggttcaagagggactcattttttgatgaagaaatggaaataccaccttgttaa tttttggcaatattattttcatttttggtctcaaccatataggattgatataaagaaattatcaaactattctttttattttctgggttatttttcaagtgtacaaa ttaattcttcgatggtaaggaatcaaatgctagagaattcatttctgatggatactcttactaagaaatttgataccataatcccaattattcctcttattc gatcattgtctaaagctcaattttgtaccgtatctgggtatcctattagtaaaccaatttggaccgatttagcggattgtgatattattaatagatttggtc ggatatgtagaaagctttctcactatcacagtggatcctcaaaaaaacagagtttgtatcgaatgaagtatatacttcgactttcatgtgccagaact ttggcccgtaaacataaaagttcagcacgcagttttttgcaaagattaagttcgggattattagaagaattctttacggaagaagaacaagttattttt Phụ lục C - 69 - ttgatctttccaaaaataatttatttttatttatatgtatcatatagagaacgtatttggtatttggatattatccgtatcaatgacttggtaaattgtttattag tcacaacataa >N9 atggaagaattacaaggatatttagaagaagatagatctcggcaacaacagtttctatatccacttctctttcaagaatatatttacgtatttgcttatg atcatgggttaaatagttcaattttttatgaaccccaaaactccttgggttatgacaataaatttagttcagtacttgtgaaacgtttaattattcgaatgt atcaaaaaaattatttgatttattcggttaatgatatttaccaaaatttatttgttgggcataacaattatttttattttaattttttttctcagattctatctgaag gttttgcagtcattgtagaaattccattttcgctgcagttaatatcttcccttgaagaaaaagaaataccaaaatctcacaatttacaatctagtcattca atatttccttttttagaggataaattattgcatttaaattatctgtccgatatactaataccctatcccgtccatatggaaatcttggtccaaatgcttcaat cctggatccaggatgttctctctttacatttattgcagttccttctccacgaatattataattggactagtctcattattccgaaaaaatctatttacgtattt tcaaaagaaaataaaagactattttggttcttatataatttatatatatatgaatacgaatttctattagtgtttccttgtaaacaatcctcttttttacgatta atatcttctggagtccttcttgagcgaatacatttttatgtaaaaatagaacatcttggagtgtgccgaattttttgtcagaagactctatggattttcaa ggatcctttcatacattatattcgatatcaaggaaaatcgattctgggttcaagagggactcattttttgatgaagaaatggaaataccaccttgttaa tttttggcaatattattttcatttttggtctcaaccatataggattgatataaagaaattatcaaactattctttttattttctgggttatttttcaagtgtacaaa ttaaatcttcgatggtaaggaatcaaatgctagagaattcatttctgatggatactcttactaagaaatttgataccataatcccaattattcctcttattc gatcattgtctaaagctcaattttgtaccgtatctgggtatcctattagtaaaccaatttggaccgatttagcggattgtgatattattaatagatttggtc ggatatgtagaaagctttctcactatcacagtggatcctcaaaaaaacagagtttgtatcgaatgaagtatatacttcgactttcatgtgccagaact ttggcccgtaaacataaaagttcagcacgcagttttttgcaaagattaagttcgggattattagaagaattctttacggaagaagaacaagttattttt ttgatctttccaaaaataatttatttttatttatatgtatcatatagagaacgtatttggtatttggatattatccgtatcaatgacttggtaaattgtttattag tcacaacataa >N10 atggaagaattacaaggatatttagaagaagatagatctcggcaacaacagtttctatatccacttctctttcaagaatatatttacgtatttgcttatg atcatgggttaaatagttcaattttttatgaaccccaaaactccttgggttatgacaataaatttagttcagtacttgtgaaacgtttaattattcgaatgt atcaaaaaaattatttgatttattcggttaatgatatttaccaaaatttatttgttgggcataacaattatttttattttaattttttttctcagattctatctgaag gttttgcagtcattgtagaaattccattttcgctgcagttaatatcttcccttgaagaaaaagaaataccaaaatctcacaatttacaatctagtcattca atatttccttttttagaggataaattattgcatttaaattatctgtccgatatactaataccctatcccgtccatatggaaatcttggtccaaatgcttcaat cctggatccaggatgttctctctttacatttattgcagttccttctccacgaatattataattggactagtctcattattccgaaaaaatctatttacgtattt tcaaaagaaaataaaagactattttggttcttatataatttatatatatatgaatacgaatttctattagtgtttccttgtaaacaatcctcttttttacgatta atatcttctggagtccttcttgagcgaatacatttttatgtaaaaatagaacatcttggagtgtgccgaattttttgtcagaagactctatggattttcaa ggatcctttcatacattatattcgatatcaaggaaaatcgattctgggttcaagagggactcattttttgatgaagaaatggaaataccaccttgttaa tttttggcaatattattttcatttttggtctcaaccatataggattgatataaagaaattatcaaactattctttttattttctgggttatttttcaagtgtacaaa ttaattcttcgatggtaaggaatcaaatgctagagaattcatttctgatggatactcttactaagaaatttgataccataatcccaattattcctcttattc gatcattgtctaaagctcaattttgtaccgtatctgggtatcctattagtaaaccaatttggaccgatttagcggattgtgatattattaatagatttggtc ggatatgtagaaagctttctcactatcacagtggatcctcaaaaaaacagagtttgtatcgaatgaagtatatacttcgactttcatgtgccagaact ttggcccgtaaacataaaagttcagcacgcagttttttgcaaagattaagttcgggattattagaagaattctttacggaagaagaacaagttattttt ttgatctttccaaaaataatttatttttatttatatgtatcatatagagaacgtatttggtatttggatattatccgtatcaatgacttggtaaattgtttattag tcacaacataa >N11 atggaagaattacaaggatatttagaagaagatagatctcggcaacaacagtttctatatccacttctctttcaagaatatatttacgtatttgcttatg atcatgggttaaatagttcaattttttatgaaccccaaaactccttgggttatgacaataaatttagttcagtacttgtgaaacgtttaattattcgaatgt atcaaaaaaattatttgatttattcggttaatgatatttaccaaaatttatttgttgggcataacaattatttttattttaattttttttctcagattctatctgaag gttttgcagtcattgtagaaattccattttcgctgcagttaatatcttcccttgaagaaaaagaaataccaaaatctcacaatttacaatctagtcattca atatttccttttttagaggataaattattgcatttaaattatctgtccgatatactaataccctatcccgtccatatggaaatcttggtccaaatgcttcaat cctggatccaggatgttctctctttacatttattgcagttccttctccacgaatattataattggactagtctcattattccgaaaaaatctatttacgtattt tcaaaagaaaataaaagactattttggttcttatataatttatatatatatgaatacgaatttctattagtgtttccttgtaaacaatcctcttttttacgatta atatcttctggagtccttcttgagcgaatacatttttatgtaaaaatagaacatcttggagtgtgccgaattttttgtcagaagactctatggattttcaa ggatcctttcatacattatattcgatatcaaggaaaatcgattctgggttcaagagggactcattttttgatgaagaaatggaaataccaccttgttaa tttttggcaatattattttcatttttggtctcaaccatataggattgatataaagaaattatcaaactattctttttattttctgggttatttttcaagtgtacaaa ttaattcttcgatggtaaggaatcaaatgctagagaattcatttctgatggatactcttactaagaaatttgataccataatcccaattattcctcttattc gatcattgtctaaagctcaattttgtaccgtatctgggtatcctattagtaaaccaatttggaccgatttagcggattgtgatattattaatagatttggtc ggatatgtagaaagctttctcactatcacagtggatcctcaaaaaaacagagtttgtatcgaatgaagtatatacttcgactttcatgtgccagaact ttggcccgtaaacataaaagttcagcacgcagttttttgcaaagattaagttcgggattattagaagaattctttacggaagaagaacaagttattttt Phụ lục C - 70 - ttgatctttccaaaaataatttatttttatttatatgtatcatatagagaacgtatttggtatttggatattatccgtatcaatgacttggtaaattgtttattag tcacaacataa >N12 atggaagaattacaaggatatttagaagaatatagatctcggcaacaacagtttctatatccacttctctttcaagaatatatttacgtatttgcttatga tcatgggttaaatagttcgattttttatgaaccccaaaactccttgggttatgacaataaatttagttcagtacttgtgaaacgtttaattattcgaatgta tcaaaaaaattattggatttattcggttaatgatatttaccaaaatatatttgttgggcataacaattatttttattttcattttttttctcagattatatctgaag gttttgcagtcattgtagaaattccattttcgctgcagttaatatcttcccttgaagaaaaagaaataccaaaatctcacaatttacaatctagtcattca atatttccttttttagaggataaattattgcatttaaattatctgtccgatatactaataccctatcccgcccatatggaaatcttggtccaaatgcttcaat cctggatccaggatgttctctctttacatttattgcagttccttctccacgaatattataattggaatagtctcattattccgaataaatctatttacgtattt tcaaaagacaataaaagactattttgtttcttatataatttatatatatatgaatatgaatttctattagtgtttccttgtaaacaatcctcttttttacgactaa tatcttctggagtccttcttgagcgaatacatttttatgtaaaaatagaacgtcttggagtgtgccgaattttttgtcagaagactctatggattttcaag gatcctttcatacattatattcgatatcaaggaaaatcgattttgggttcaagagggactcattttttgatgaagaaatggaaataccatcttgttcattt ttggcaatatcattttcatttttggtctcaaccatataggattgatataaagaaattatcaaactattctttttattttcttggttatttttcaagtgtacaaatg aattcttcgatagtaaggaatcaaatgctagagaattcatttctagtggatactcttactaagaaatttgatactagaatcccaattattcctcttattcg atcattgtctaaagctcaattttgtactgtatatgggtatcccattagtaaaccaattttgaccgatttagcagattgtgatattattaatagatttggtcg gatatgtagaaaactttctcactatcatagtggatcctcaaaaaaacagagtttgtatcgaatgaagtatatacttcgactttcatgtgccagaacttt ggctcgtaaacataaaagttcagcacgcagttttttgcaaagattaagttcgggattattagaagaattctttacggaagaagaacaagttatttcttt gatctttccaaaaataacttctttttatttatatggatcatatagagaacgtatttggtattttgatattatccgtatcaatgacctggtaaatagtttattag tcacgacataa >N13 atggaagaattacaaggatatttagaagaagatagatctcggcaacaacagtttctatatccacttctctttcaagaatatatttacgtatttgcttatg atcatgggttaaatagttcaattttttatgaaccccaaaactccttgggttatgacaataaatttagttcagtacttgtgaaacgtttaattattcgaatgt atcaaaaaaattatttgatttattcggttaatgatatttaccaaaatatatttgtcgggcataacaattatttttattttaattttttttctcagattctatctgaa ggttttgcagtcattgtagaaattccattttcgctgcagttaatatcttcccttgaagaaaaagaaataccaaaatctcacaatttacaatctagtcattc aatatttccttttttagaggataaattattgcatttaaattatctgtccgatatactaataccctatcccgtccatatggaaatcttggtccaaatgcttcaa tcctggatccaggatgttctctctttacatttattgcagttccttctccacgaatattataattggaatagtctcattattccgaaaaaatctatttacgtatt ttcaaaagaaaataaaagactattttggttcttatataatttatatatatatgaatacgaatttctattagtgtttccttgtaaacaatcctcttttttacgatt aatatcttctggagtccttcttgagcgaatacatttttatgtaaaaatagaacatcttggagtgtgccgaattttttgtcagaagactctatggattttca aggatcctttcatacattatattcgatatcaaggaaaatcgattctgggttcaagagggactcattttttgatgaagaaatggaaataccaccttgtta atttttggcaatattattttaatttttggtctcaaccatataggattgatataaagaaattatcaaactattctttttattttctgggttatttttcaagtgtacaa attaattcttcgatggtaaggaatcaaatgctagagaattcatttctaatggatactcttactaagaaatttgatactataatcccgattattcctcttatt cgatcattgtttaaagctcaattttgtaccgtatctgggtatcctattagtaaaccaatttggaccgatttagcggattgtgatattattaatagatttggt cggatatgtagaaagctttctcactatcacagtggatcctcaaaaaaacagagtttgtatcgaatgaagtatatacttcgactttcatgtgccagaac tttggcccgtaaacataaaagttcagcacgcagttttttgcaaagattaagttcgggattattagaagaattctttacggaagaagaacaagttatttt tttgatctttccaaaaataatttctttttatttatatggatcatatagagaacgtatttggtatttggatattatccgtatcaatgacctggtaaattgtttatta gtcacgacataa >N18 atggaagaattacaaggatatttagaagaagatagatctcggcaacaacagtttctatatccacttctctttcaagaatatatttacgtatttgcttatg atcatgggttaaatagttcaattttttatgaaccccaaaactccttgggttatgacaataaatttagttcagtacttgtgaaacgtttaattattcgaatgt atcaaaaaaattatttgatttattcggttaatgatatttaccaaaatatatttgtcgggcataacaattatttttattttaattttttttctcagattctatctgaa ggttttgcagtcattgtagaaattccattttcgctgcagttaatatcttcccttgaagaaaaagaaataccaaaatctcacaatttacaatctagtcattc aatatttccttttttagaggataaattattgcatttaaattatctgtccgatatactaataccctatcccgtccatatggaaatcttggtccaaatgcttcaa tcctggatccaggatgttctctctttacatttattgcagttccttctccacgaatattataattggaatagtctcattattccgaaaaaatctatttacgtatt ttcaaaagaaaataaaagactattttggttcttatataatttatatatatatgaatacgaatttctattagtgtttccttgtaaacaatcctcttttttacgatt aatatcttctggagtccttcttgagcgaatacatttttatgtaaaaatagaacatcttggagtgtgccgaattttttgtcagaagactctatggattttca aggatcctttcatacattatattcgatatcaaggaaaatcgattctgggttcaagagggactcattttttgatgaagaaatggaaataccaccttgtta atttttggcaatattattttcatttttggtctcaaccatataggattgatataaagaaattatcaaactattctttttattttctgggttatttttcaagtgtacaa attaattcttcgatggtaaggaatcaaatgctagagaattcatttctaatggatactcttactaagaaatttgatactataatcccgattattcctcttatt cgatcattgtttaaagctcaattttgtaccgtatctgggtatcctattagtaaaccaatttggaccgatttagcggattgtgatattattaatagatttggt cggatatgtagaaagctttctcactatcacagtggatcctcaaaaaaacagagtttgtatcgaatgaagtatatacttcgactttcatgtgccagaac tttggcccgtaaacataaaagttcagcacgcagttttttgcaaagattaagttcgggattattagaagaattctttacggaagaagaacaagttatttt Phụ lục C - 71 - tttgatctttccaaaaataatttctttttatttatatggatcatatagagaacgtatttggtatttggatattatccgtatcaatgacctggtaaattgtttatta gtcacgacataa >N19 atggaagaattacaaggatatttagaagaagatagatctcggcaacaacagtttctatatccacttctctttcaagaatatatttacgtatttgcttatg atcatgggttaaatagttcaattttttatgaaccccaaaactccttgggttatgacaataaatttagttcagtacttgtgaaacgtttaattattcgaatgt atcaaaaaaattatttgatttattcggttaatgatatttaccaaaatatatttgtcgggcataacaattatttttattttaattttttttctcagattctatctgaa ggttttgcagtcattgtagaaattccattttcgctgcagttaatatcttcccttgaagaaaaagaaataccaaaatctcacaatttacaatctagtcattc aatatttccttttttagaggataaattattgcatttaaattatctgtccgatatactaataccctatcccgtccatatggaaatcttggtccaaatgcttcaa tcctggatccaggatgttctctctttacatttattgcagttccttctccacgaatattataattggaatagtctcattattccgaaaaaatctatttacgtatt ttcaaaagaaaataaaagactattttggttcttatataatttatatatatatgaatacgaatttctattagtgtttccttgtaaacaatcctcttttttacgatt aatatcttctggagtccttcttgagcgaatacatttttatgtaaaaatagaacatcttggagtgtgccgaattttttgtcagaagactctatggattttca aggatcctttcatacattatattcgatatcaaggaaaatcgattctgggttcaagagggactcattttttgatgaagaaatggaaataccaccttgtta atttttggcaatattattttcatttttggtctcaaccatataggattgatataaagaaattatcatacaattctttttattttctgggttatttttcaagtgtacca attaattcttcgatggtaagaaatcaaatgctagagaattcatttctaatggatactcttactaagaaatttgatactataatcccgattattcctcttattc gatcattgtttaaagctcaattttgtaccgtatctgggtatcctattagtaaaccaatttggaccgatttagcggattgtgatattattaatagatttggtc ggatatgtagaaagctttctcactatcacagtggatcctcaaaaaaacagagtttgtatcgaatgaagtatatacttcgactttcatgtgccagaact ttggcccgtaaacataaaagttcagcacgcagttttttgcaaagattaagttcgggattattagaagaattctttacggaagaagaacaagttattttt ttgatctttcctaaaataatttctttttatttatatggatcatatagagaacgtatttggtatttggatattatccgtatcaatgacctggtaaattgtttatta gtcacgacataa >N20 atggaagaattacaaggatatttagaagaagatagatctcggcaacaacagtttctatatccacttctctttcaagaatatatttacgtatttgcttatg atcatgggttaaatagttcaattttttatgaaccccaaaactccttgggttatgacaataaatttagttcagtacttgtgaaacgtttaattattcgaatgt atcaaaaaaattatttgatttattcggttaatgatatttaccaaaatatatttgtcgggcataacaattatttttattttaattttttttctcagattctatctgaa ggttttgcagtcattgtagaaattccattttcgctgcagttaatatcttcccttgaagaaaaagaaataccaaaatctcacaatttacaatctagtcattc aatatttccttttttagaggataaattattgcatttaaattatctgtccgatatactaataccctatcccgtccatatggaaatcttggtccaaatgcttcaa tcctggatccaggatgttctctctttacatttattgcagttccttctccacgaatattataattggactagtctcattattccgaaaaaatctatttacgtatt ttcaaaagaaaataaaagactattttggttcttatataatatatatatatatgaatacgaatttctattagtgtttccttgtaaacaatcctcttttttacgatt aatatcttctggaatccttcttgagcgaatacatttttatgtaaaaatagaacatcttggagtgtgccgaattttttgtcagaagactctatggattttca aggatcctttcatacattatattcgatatcaaggaaaatcgattctgggttcaagggggactcattttttgatgaagaaatggacataccaccttgtta atttttggcaatattattttcattttaggtctcaaccatataggattgatataaagaaattatcaaactattctttttattttctgggttatttttcaagtgtaca aattaattcttcgatggtaaggaatcaaatgctagagaattcatttctaatggatactcttactaagaaatttgataccataatcccaattattcctcttat tcgatcattgtctaaagctcaattttgtaccgtatctgggtatcctattagtaaaccaatttggaccgatttagcggattgtgatattattaatagatttgg tcggatatgtagaaagctttctcactatcacagtggatcctcaaaaaaacagagtttgtatcgaatgaagtatatacttcgactttcatgtgccagaa ctttggcccgtaaacataaaagttcagcacgcagttttttgcaaagattaagttcgggattattagaagaattttttacggaagaagaacaagttattt ttttgatctttccaaaaataatttctttttatttatatggatcatatagagaacgtatttggtatttggatattatccgtatcaatcacctggtaaattgtttatt agtcacaacataa >N22 atggaagaattacaaggatatttagaagaagatagatctcggcaacaacagtttctatatccacttctctttcaagaatatatttacgtatttgcttatg atcatgggttaaatagttcaattttttatgaaccccaaaactccttgggttatgacaataaatttagttcagtacttgtgaaacgtttaattattcgaatgt atcaaaaaaattatttgatttattcggttaatgatatttaccaaaatatatttgttgggcataactattatttttattttaattttttttctcagattctatctgaag gttttgcagtcattgtagaaattccattttcgctgcagttaatatcttcccttgaagaaaaagaaataccaaaatcttacaatttacaatctagtcattca atatttccttttttagaggataaattattgcatttaaattatctatccgatatactaataccctatcccgtccatatggaaatcttggtccaaatgcttcaat cctggatccaggatgttctctctttacatttattgcagttccttctccacgaatattataattggaatagtctcattattccgaaaaaatctatttacgtattt tcaaaagaaaataaaagactattttggttcttatataatttatatatatatgaatacgaatttctattagtgtttccttgtaaacaatcctcttttttacgatta atatcttctggagtccttcttgagcgaatacatttttatgtaaaaatagaacatcttggagtgtgtcgaattttttgtcagaagactctatggattttcaag gatcctttcatacattatattcgatatcaaggaaaatcgattctgggttcaagagggactcattttttgatgaagaaatggaaataccaccttgttaatt tttggcaatattattttcatttttggtctagaccatataggattgatataaagaaattatcaaattattctttttattttctgggttatttttcaagtgtacaaatt aattcttcgatggtaaggaatcaaatgctagagaattcatttctaatggatactcttactaagaaatttgatactataatcccaattattcctcttattcga tcattgtctaaagctcaattttgtaccgtatctgggtatcctattagtaaaccaatttggaccgatttagcggattgtgatattattaatagatttggtcg gatatgtagaaagctttctcactatcacagtggatcctcaaaaaaacagagtttgtatcaaatgaagtatatacttcgactttcatgtgccagaacttt ggcccgtaaacataaaagttcagtacgcagttttttgcaaagattaagttcgggattattagaagaattctttacggaagaagaacaagttattttttt Phụ lục C - 72 - gatctttccaaaaataatttctttttatttatatggatcatatagagaacgtatttggtatttggatattatccgtatcaatgacccggtaaattgtttattag tcacgacataa >N26 atggaagaattacaaggatatttagaagaagatagatctcggcaacaacagtttctatatccacttctctttcaagaatatatttacgtatttgcttatg atcatgggttaaatagttcaattttttatgaaccccaaaactccttgggttatgacaataaatttagttcagtacttgtgaaacgtttaattattcgaatgt atcaaaaaaattatttgatttattcggttaatgatatttaccaaaatatatttattgggcataacaattatttttattttcattttttttctcagattctatctgaa ggttttgcagtcattgtagaaactccattttcgctgcagttaatatcttcccttgaagaaaaagaaataccaaaatctcacaatttacaatctagtcatt caatatttccttttttagaggataaattattgcatttaaattatctgtccgatatactaataccctatcccgtccatatggaaatcttggtccaaatgcttca atcctggatccaggatgttctctctttacatttattgcagttccttctatacgaatattataattggaatagtctcattatttcgaaaaaatctatttacgtat tttcaaaagaaaataaaagattattttggttcttatataatttatatatatatgaatacgaatttctattagtgtttccttgtaaacaatcctcttttttacgatt aatatcttctggagtccttcttgagcgaatacatttttatgtaaaaatagaacatcttggagtgtgccgaattttttgtcagaagactctatggattttca aggatcctttcatacattatattcgatatcaaggaaaatcgattctgggttcaagagggactcattttttgatgaagaaatggaaataccaccttgtta atttttggcaatattattttcatttttggtctcaaccatataggattgatataaagaaattatcaaactattcttttttttttctgggttatttttcaagtgtacaa attaattctttgatggtaaggaatcaaatgctagagaattcatttctaatggatactcttactaagaaatttgatactataatcccaattattcctcttattc gatcattgtctaaagctcaattttgtaccgtatctgggtatcctattagtaaaccaatttggaccgatttagcggattgtgatattattaatagatttggtc ggatatgtagaaagctttctcactatcacagtggatcctcaaaaaaacagagtttgtatcgaatgaagtatatacttcgactttcatgtgccagaact ttggcccgtaaacataaaagttcagcacgcagttttttgcaaagattaagttcgggattattagaagaattctttacggaagaagaacaagttattttt ttgatctttccaaaaataatttctttttatttatacggatcatatagagaacgtatttggtatttggatattatccgtatcaatgacctggtaaattgtttatta gtcacgacataa >N29 atggaagaattacaaggatatttagaagaagatagatctcggcaacaacagtttctatatccacttctctttcaagaatatatttacgtatttgcttatg atcatgggttaaatagttcaattttttatgaaccccaaaactccttgggttatgacaataaatttagttcagtacttgtgaaacgtttaattattcgaatgt atcaaaaaaattatttgatttattcggttaatgatatttaccaaaatatatttgtcgggcataacaattatatttattttaattttttttctcagattctatctgaa ggttttgcagtcattgtagaaattccattttcgctgcagttaatatcttcccttgaagaaaaagaaataccaaaatctcacaatttacaatctagtcattc aatatttccttttttagaggataaattattgcatttaaattatctgtccgatatactaataccctatcccgtccatatggaaatcttggtccaaatgcttcaa tcctggatccaggatgttctctctttacatttattgcagttccttctccacgaatattataattggaatagtctcattattccgaaaaaatctatttacgtatt ttcaaaagaaaataaaagactattttggttcttatataatttatatatatatgaatacgaatttctattagtgtttccttgtaaacaatcctcttttttacgatt aatatcttctggagtccttcttgagcgaatacatttttatgtaaaaatagaacatcttggagtgtgccgaattttttgtcagaagactctatggattttca aggatcctttcatacattatattcgatatcaaggaaaatcgattctgggttcaagagggactcattttttgatgaagaaatggaaataccaccttgtta atttttggcaatattattttcatttttggtctcaaccatataggattgatataaagaaattatcaaactattctttttattttctgggttatttttcaagtgtacaa attaattcttcgatggtaaggaatcaaatgctagagaatttatttctaatggatactcttactaagaaatttgatactataatcccgattattcctcttattc gatcattgtttaaagctcaattttgtaccgtatctgggtatcctattagtaaaccaatttggaccgatttagcggattgtgatattattaatagatttggtc ggatatgtagaaagctttctcactatcacagtggatcctcaaaaaaacagagtttgtatcgaatgaagtatatacttcgactttcatgtgccagaact ttggcccgtaaacataaaagttcagcacgcagttttttgcaaagattaagttcgggattattagaagaattctttacggaagaagaacaagttattttt ttgatctttccaaaaataatttctttttatttatatggatcatatagagaacgtatttggtatttggatattatccgtatcaatgacctggtaaattgtttatta gtcacgacataa Phụ lục C ... vùng ITS M: thang 100 bp 3.2.1.2 Phân tích trình tự vùng ITS Trình tự ITS 25 mẫu ngải (xem thêm phụ lục C) phân tích với 25 trình tự ITS GenBank Như vậy, tổng cộng có 53 trình tự ITS phân tích, ... Kb 3.2.2.2 Phân tích trình tự vùng trnK - matK Trình tự trnK - matK 19 mẫu thực nghiệm phân tích với 20 mẫu trình tự trnK - matK GenBank Như vậy, tổng cộng có 39 trình tự trnK phân tích, gồm 36... pc 2.1 2.3.7.2 Phân tích trình tự dựng phát sinh lồi dựa vào trình tự ITS matK Trình tự 25 mẫu ngải, 25 lồi thuộc nhóm lồi thuộc nhóm ngồi (Bảng 2.1) so sánh với Phân tích trình tự dựng phát sinh