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General procedures+ Specimen preparation+ Specimen volume: 10-15 mL+ Centrifugation: 5 mins and RCF of 400+ Volume of sediment examined: 20 uL 0.02 mL+ Examination of the sediment: both
PHÂN TÍCH NƯỚC TIỂU, CẶN LẮNG NƯỚC TIỂU TS Nguyễn Hữu Tùng I Introduction History and importance + Uroscopy Urinalysis Past + Physical properties Modern + Physical properties + Chemical analysis + Microscopic examination of urine sediment ❖Urine Formation ❖Urine Composition ❖Urine Volume II Microscopic Examination of Urine Urinary sediment: insoluble materials present in the urine + RBCs + WBCs + Epithelial cells + Casts + Bacteria, yeast + Parasite + Mucus + Spermatozoa + Crystals + Artifacts Macroscopic Screening Preparation and Examination of the Urine Sediment 2.1 General procedures + Specimen preparation + Specimen volume: 10-15 mL + Centrifugation: mins and RCF of 400 + Volume of sediment examined: 20 uL (0.02 mL) + Examination of the sediment: both low (10x) and high (40x) power + Reporting of the microscopic examination - Protocol Result: Casts: low-power field and average of 10 fields RBCs, WBCs: high-power field and average of 10 fields Epithelial cells, crystals, and other elements: (+), (++), (+++), (++++) 2.2 Sediment Examination Techniques 2.2.1 Sediment Stains