Tạo bộ sưu tập giống nấm họ linh chi ganodermataceae giai đoạn 2

BAN QUẢN LÝ KHU NÔNG NGHIỆP CÔNG NGHỆ CAO TP HCM TRUNG TÂM NGHIÊN CỨU VÀ PHÁT TRIỂN NÔNG NGHIỆP CÔNG NGHỆ CAO BÁO CÁO NGHIỆM THU TẠO BỘ SƯU TẬP GIỐNG NẤM HỌ LINH CHI (HỌ Ganodermataceae) - GIAI ĐOẠN KS Nguyễn Thị Ngọc Sương Thành phố Hồ Chí Minh, tháng 01/2018 BAN QUẢN LÝ KHU NÔNG NGHIỆP CÔNG NGHỆ CAO TP HCM TRUNG TÂM NGHIÊN CỨU VÀ PHÁT TRIỂN NÔNG NGHIỆP CÔNG NGHỆ CAO BÁO CÁO NGHIỆM THU (Đã chỉnh sửa theo góp ý Hội đờng nghiệm thu) TẠO BỘ SƯU TẬP GIỐNG NẤM HỌ LINH CHI (HỌ Ganodermataceae) - GIAI ĐOẠN CƠ QUAN CHỦ TRÌ (Ký tên, đóng dấu xác nhận) CHỦ NHIỆM ĐỀ TÀI (Ký tên) Thành phố Hồ Chí Minh, tháng 01/2018 c , Lò Gò Xa Mát ,B , Amauroderma subresinosum), T0300 (Amauroderma rugosum), T0302 (Ganoderma sp2.), T0317 (Ganoderma fulvellum Amauroderma rugosum Amauroderma subresinosum, Ganoderma australe amoiense, Amauroderma Ganoderma aff gibbosum, Ganoderma aff mastoporum, Ganoderma aff lobatum, Ganoderma capense, Ganoderma cochlear, Ganoderma flexipes, Ganoderma fulvellum, Ganoderma philippii, Ganoderma resinaceum, Humphreya aff lloydii, Humphreya coffeatum Trong c Trang i BM20-QT.QLKH qu c Trang ii BM20-QT.QLKH Trang TÓM T T i M C L C iii DANH SÁCH CÁC CH VI T T T v DANH SÁCH B NG vi TÀI vii M U NG QUAN NGHIÊN C U 1.1 Gi i thi u v h Ganodermataceae 1.2 L ch s phân lo i h th ng h c h n m Linh Chi th gi i 1.3 Ganoderma 12 1.3.1 Ngu n g c, phân lo i s phân b Linh Chi 12 1.3.2 m hình thái v trí phân lo i 14 1.3.3 Chu trình s m ni tr ng 15 1.3.4 Thành ph n hóa h c ch y u c a n m Linh Chi 19 1.3.5 Vai trò c a n i v i s c kh e 22 1.4 Amauroderma 27 m sinh h c 27 1.4.2 M t s nghiên c u v Amauroderma 28 1.5 Haddowia 29 1.6 Humphreya Steyeart (1972) 30 I DUNG NGHIÊN C U 35 2.1 Th i g m nghiên c u 35 35 35 2.2 V t li u thi t b 35 Trang iii BM20-QT.QLKH 35 35 2.3 N i dung nghiên c u 36 c hi n x lý th ng kê 36 T QU VÀ TH O LU N 44 3.1 Kh o sát m t s 3.2 Kh u ki n b o qu i v i gi ng n m b ng d ng c a gi ng n m thu th 3.3 Phân lo i m u n m b c ab p 44 c 50 p 53 p 67 T LU N VÀ KI N NGH 124 4.1 K t lu n 124 4.2 Ki n ngh 124 TÀI LI U THAM KH O 125 Trang iv BM20-QT.QLKH CZA Czapek dox agar MEA Malt extract agar PDA Potato dextrose agar YMA Yeast malt extract agar ctv c viên TGBQ MT NT Trang v BM20-QT.QLKH B ng T a b ng Trang 44 51 60 62 Trang vi BM20-QT.QLKH Giai 01/2017 - 12/2017 252.589.000 - ánh - - odermataceae Trang vii BM20-QT.QLKH Bidoup Núi Bà Ninh; 10 m T , Ganodermataceae) Trang BM20-QT.QLKH Tên khoa phân T0310 Amauroderma amoiense phân danh minh Trình rDNA (ITS2) AGGCAAAGGCTAGTATACCTTGCTTGCGACGCTGC GCTATAAGCGGGTGCTTCTCCTGGCCGAGCCTTTT ACACCGCTTGTGGACACAATGCTGTTCGAGGTGTA GGCGTAGGGGAGGTCAATTTTCCCATGTCTTTCCC TTGAGACGGCCTCACTTCCACCAGAAAGGCCCGGA GAGTGTAAAACCCTCTCGAGTCTCTCAAAGCATCC GGCTACCGCGTGCCTGGAACCCGTGACCTGGATGC TCTACGTGACTCGAAATGATTTGTGGTGAAACGAC CGAATATATCAACAACTTTCTAGATCTGGAGCTGA ACATAGGGGAAATTTGCTTGCCCCTGACACATTTA Trang 115 BM20-QT.QLKH CCGCACAGCCCTCAATCCTACAAGCACTTGGAAAT CGTCTGCAAGAGGAAGACGTGCGGAGTTTTCCCGT GTGTCACTAAACACGTTTTTATCCAGACTTGACAT CCGCCGGTTCACCTACCGAAACTCGGTGTGATAAT GTCTACGCCGCGACCGTGAAGCGTTTGGCAAGCTT CTAACAGTCTCAGTTGGAGACAGCTTTATGACCTC TGACCTCAAATCAGGTAGGACTACCCGCTGAACTT AAGCATATCAATAAGCGGAAGGAAAAATT C C C 0 C C Môi nuôi C Potato dextrose agar, Yeast Malt extract agar, Malt extract agar Trang 116 BM20-QT.QLKH Tên khoa phân T0316 Amauroderma rugosum phân danh minh Trình rDNA (ITS2) C PDA glycerol 10%, 40C C YMA glycerol 10%, 40C C 0 C C Môi nuôi C Potato dextrose agar, Yeast Malt extract agar, Malt extract agar Trang 117 BM20-QT.QLKH Tên khoa phân T0317 Ganoderma fulvellum h Tây Ninh phân danh minh Trình rDNA (ITS2) GGGCTTTTCGAGTCTTGACTGGGTTGTAGCTGGC CTTCCGAGGCATGTGCACGCCCTGCTCATCCACT CTACACCTGTGCACTTACTGTGGGCTTCAGATCG TAAAGCAGGGCCCTTCACCGGGCTTTGCAGGAC GTGTCTGTGCCTGCGTTTATCACAAACTCTATAA AGTATCAGAATGTGTATTGCGATGTAACGCATCT ATATACAACTTTCAGCAACGGATCTCTTGGCTCT CGCATCGATGAAGAACGCAGCGAAATGCGATAA GTAATGTGAATTGCAGAATTCAGTGAATCATCG AATCTTTGAACGCACCTTGCGCTCCTTGGTATTC CGAGGAGCATGCCTGTTTGAGTGTCATGAAATC ATCAACCTACAAGCCTTTGCGGTTTTGTAGGCTT Trang 118 BM20-QT.QLKH GGACTTGGAGGCTTGTCGGCCGTTCTTGGTCGGC TCCTCTTAAATGCATTAGCTTGGTTCCTTGCGGA TCGGCTCTCGGTGTGATAATGTCTACGCCGCGAC CGTGAAGCGTTTGGCAAGCTTCTAACAGTCTCA GTTGGAGACAGCTTTATGACCTCTGACCTCAAAT CAGGTAGGACTACCCGCTGAACTTAAGCATATC AATAGGCCGAAGGAA C C C C Potato dextrose agar, Yeast Malt extract agar, Malt extract agar Môi nuôi Trang 119 BM20-QT.QLKH T0409 Tên khoa phân Polyporaceae (Formitoptaceae) phân danh minh Trình rDNA (ITS2) C PDA glycerol 10%, 40C C YMA glycerol 10%, 40C C 0 C C Môi nuôi C Potato dextrose agar, Yeast Malt extract agar, Malt extract agar Trang 120 BM20-QT.QLKH Tên khoa phân T0414 Ganoderma aff gibbosum (Ness) Pat phân danh minh Trình rDNA (ITS2) GTGGGTTGTAGCTGGCCTTCCGAGGCATGTGCAC GCCCCGCTCATCCACTCTACACCTGTGCACTTAC TGTGGGCTTCAGGTCGTGAAGCGAGCCCTTTACC GGGCTCGCGGAGCGCATCTGTGCCTGCGTTTATC ACAAACCCTATAAAGTAATCGAATGTGTATTGC GATGTAACGCATCTATATACAACTTTCAGCAACG GATCTCTTGGCTCTCGCATCGATGAAGAACGCAG CGAAATGCGATAAGTAATGTGAATTGCAGAATT CAGTGAATCATCGAATCTTTGAACGCACCTTGCG CTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAG TGTCATGAAATCTTCAACCTACAAGCTTCTTAAT TGAGTTTGTTAGGCTTGGACTTGGAGGCTTGTCG GTCCTTGTAGGTCGGCTCCTCTTAAATGCATTAG CTTGGTTCCTTGCGGATCGGCTTGTCGGTGTGAT AATGTCTACGCCGCGACCGAGAAGCGTTTTGGCT GAGCTTCTAACCGTCTTGTTATAAAGACAACATT ATGACCTCTGACCTCAAATCAGGTAGGA Trang 121 BM20-QT.QLKH C PDA glycerol 10%, 40C C YMA glycerol 10%, 40C C 0 C C Môi nuôi C Potato dextrose agar, Yeast Malt extract agar, Malt extract agar Trang 122 BM20-QT.QLKH Tên khoa N phân V0110 Ganoderma resinaceum phân danh minh Trình rDNA (ITS2) AGGGGTTGTAGCTGGCCTTCCGGGGCATGTGCA CATCCTGCTCAATCCACTCTACACCTGTGCACTT ATTGTGGGTTCCTGTGAGGTGCTGAGCAGGCCTC TTTTGGCCTGTGAGGCCCTTCTGGGCCTGCGTTT TTATTATCGACTCCGAAGTAATCAGAACGTGCAA TGCGCGATGTAACGCATTTATATACAACTTTCAG CAACGGATCTCTTGGCTCTCGCATCGATGAAGAA CGCAGCGAAATGCGATAAGTAATGTGAATTGCA GAATTCAGTGAATCATCGAATCTTTGAACGCACC TTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGT TTGAGTGTCATGAATTCCTCAACTTGCAGGCCTT TGTGGTTTGTAAGGTTGGACTTTGGAGGTGTTGT CGGCCGGGGTGGTTGGCTCCTCTTAAATGCATTA GCTCGGTTCCTTGTGGATCGGCTCTCGGTGTGAT AATGTCTACGCTGCGGCCGTGAAGCGTTTGGCG GGCTTCTTGAACCGTCCTTTGTTTGGATAATTTAT TGACCTCTGACCTCAAATCAGGTA C PDA glycerol 10%, 40C C YMA glycerol 10%, 40C C , 40C Trang 123 BM20-QT.QLKH C Môi nuôi C Potato dextrose agar, Yeast Malt extract agar, Malt extract agar T LU N VÀ KI N NGH 4.1 K t lu n - - Amauroderma subresinosum), T0300 (Amauroderma rugosum), T0302 (Ganoderma sp2), T0317 (Ganoderma fulvellum - dermataceae 4.2 Ki n ngh - - Trang 124 BM20-QT.QLKH Ti ng Vi t [1] (2008), -14 [2] [3] N [4] - -36 [5] (2002), , [6] ctv (1993), , Tài [7] [8] [9] i Liên (1983), [10] Vi [11] [12] Suy (2009) Ganoderma colosum, T chí K , 55, Tr 25-32 Trang 125 BM20-QT.QLKH [13] Lê Xuân Thám (1996a), , nam [14] Lê Xuân Thám (1996b), [15] Lê Xuân Thám (2005), - - , 31/05(1154) [16] Lê Xuân Thám (2012), [17] Lê Duy [18] Ahmed I and Lee T.S (2007), Screening of antibacterial and antifungal activities from Korean wild mushrooms, World journal of Agricultural Sciences, 3(3), pp 316-321 [19] Alexopoulos, C.J., Mims, C.W (1979), Introductory Mycology, John Wiley & Sons, New Jersey [20] Benzie I F F, Wachtel-Galor S (2009), Biomarkers of long-term vegetarian diets, Adv Clin Chem, 47, pp 169 220 [21] Bisen P.S., (2009), Ganoderma lucidum: A Potent Pharmacological Macrofungus, Current Pharmaceutical Biotechnology, 10(8), pp.717-742 [22] Boh, B (2013), Ganoderma lucidum: a potential for biotechnological production of anti-cancer and immunomodulatory drugs,Recent Patents on AntiCancer Drug Discovery, 8(3), pp 255-287 [23] Cao L Z, Lin Z B (2002), Regulation on maturation and function of dendritic cells by Ganoderma lucidum polysaccharides, Immunol Lett, , pp 163 169 Trang 126 BM20-QT.QLKH [24] Chiu S W, Wang Z M, Leung T M, Moore D (2000), Nutritional value of Ganoderma extract and assessmentof its genotoxicity and antigenotoxicity using comet assays of mouse lymphocytes, Food Chem Toxicol, [25] Collins A R (2005), Antioxidant intervention as a route to cancer prevention, Eur J Cancer, [26] , pp 173 178 1923 1930 Amauroderma and Ganoderma Co Beihefte zur Nov Hedw., 75, pp.1-182 [27] Haralampidis K, Trojanowska M, Osbourn AE (2002), Biosynthesis of triterpenoid saponins in plants, Adv Biochem Eng Biotechnol, 75, pp 31 49 [28] Hikino H, Konno C, Mirin Y, Hayashi T (1985), Isolation and hypoglycemic activity of ganoderans A and B, glycans of Ganoderma lucidum fruit bodies, Planata Med,4, pp 339 340 [29] Hikino H, Ishiyama M, Suzuki Y, Konno C (1989), Mechanisms of hypoglycemic activity of ganoderan B: A glycan of Ganoderma lucidum fruit body, Planta Med,55, pp 423 428 [30] Kim, B., Hathaway, T.R., and Loeb, L.A (1996), Human immunodeficiency virus reverse transcriptase: Functional mutants obtained by random mutagenesis coupled with genetic selection in Escherichia coli, J Biol Chem., 271, pp 4872 4878 [31] Kolesnikova O P, Tuzova M N, Kozlov V A (1997), Screening of immunoactive properties of alkanecarbonic acid derivatives and germaniumorganic compounds in vivo, Immunologiya, 10, pp 36 38 [32] Lee J M, Kwon H, Jeong H, editors et al (2001), Inhibition of lipid peroxidation and oxidative DNA damage by Ganoderma lucidum, Phytother Res, 15, pp 245 249 [33] Ma C, Guan S H, Yang M, Liu X, Guo D A (2008), Differential protein expression in mouse splenic mononuclear cells treated with polysaccharides from spores of Ganoderma lucidum, Phytomedicine, 15, pp 268 276 [34] Mahato S B, Sen S Advances in triterpenoid research, 1990- 1994, Phytochemistry,44(7), 1185 1236 Trang 127 BM20-QT.QLKH [35] Mau, J.L, H.C Lin and C.C Chen(2001), Non-volatile components of several medicinal mushrooms,Food Research International, 34, pp 521 526 [36] Mino Y, Ota N, Sakao S, Shi momura S (1980), Determination of germanium in medicinal plants by atomic absorption spectrometry with electrothermal atomization Chem Pharm Bull, [37] Paterson R R biofactory, Phytochemistry, [38] , pp 2687 2691 (2006), Ganoderma-a therapeutic fungal (18), pp 1985 2001 Rajesh K, Dhanasekaran D and Panneerselvam A (2004), Isolation and taxonomic characterization of medicinal mushroom Ganoderma spp., Academia Journal of Microbiology Research, 2(2), pp 61-70 [39] Shi Y L, James A E, Benzie I F, Buswell J A (2002), Mushroom-derived preparations in the prevention of H2O2-induced oxidative damage to cellular DNA, Teratog Carcinog Mutagen, [40] , pp 103 111 Smith, B J and Sivasithamparam, K (2000), Internal transcribed spacer ribosomal DNA sequence analysis for species of Ganoderma from Australia, Mycological Research, 104, pp 943-951 [41] Tomoda M, Gonda R, Kasahara Y, Hikino H (1986), Glycan structures of ganoderans B and C, hypoglycemic glycans of Ganoderma lucidum fruit bodies, Phytochemistry, 25, pp 2817 2820 [42] Xuan Tham Le, Quoc Hung Nguyen Le, Ngoc Duong Pham, Van Hop Duong, Bryn T M Dentinger and Jean-Marc Moncalvo (2012), Tomophagus cattienensissp nov., a new Ganodermataceaespecies from Vietnam: Evidence from morphologyand ITS DNA barcodes, Mycol Progress, 11, pp 775 780 [43] Wachtel-Galor S., J Yuen, J.A Buswell and I.F.F Benzie (2011), Chapter Ganoderma lucidum (Lingzhi or Reishi) A Medicinal Mushroom, CRC Press,Florida, pp.175-199 [44] Wang Chi, Zh Y Hua (1994), A new kind of preparation of Ganoderma lucidum in treating 35 patients with coronary cardiac disease, Proc 94 Inter Sym On Ganoderma Res., Beijing, China, pp 58-9 Trang 128 BM20-QT.QLKH [45] Wang S Y, Hsu M L, Hsu H C, editors et al (1997), The anti-tumor effect of Ganoderma lucidum is mediated by cytokines released from activated macrophages and T lymphocytes, Int J Cancer, 70, pp 699 705 [46] Wasser S P, Coates P, Blackman M, Cragg G, Levine M, Moss J, White J (2005), Reishi or Lingzhi (Ganoderma lucidum), Encyclopedia of Dietary Supplements, Marcel Dekker, New York, pp 680 690 [47] Wu Xingliang Dai Yucheng (2014) Colored illustrations of Ganodermataceae of China, Science Press, Beijing, China [48] Zhang Q H, Lin Z B (1999), Antitumor activity of Ganoderma lucidum (Curt.: Tr.) P.Karst (Lingzhi) (Aphyllophoromycetideae) polysaccharides is related to tumor necrosis factor-alpha and interferongamma, Int J Med Mushrooms, 1, pp 207 215 [49] Zhou X, Lin J, Yin Y, Zhao J, Sun X, Tang K (2007), Ganodermataceae: Natural products and their related pharmacological functions, Am J Chin Med, 35, 559 74 [50] a taxonomic mini-review, Mycology, (1), pp Trang Web [51] Trang 129 BM20-QT.QLKH