nghiên cứu xác lập các điều kiện thích hợp để cố định nấm men saccharomyces cerevisiae trong gel alginate và trên cellulose vi khuẩn nhằm ứng dụng lên rượu vang nho

ĐẠI HỌC QUỐC GIA TP. HỒ CHÍ MINH I HC BÁCH KHOA TÔN N MINH NGUYT  SACCHAROMYCES CEREVISIAE    Chuyên ngành: Chế biến thực phẩm và đồ uống Mã số chuyên ngành: 62540201 TÓM TT LUN ÁN TI THUT TP. HỒ CHÍ MINH, NĂM 2014 Công trình được hoàn thành tại: i hc Bách Khoa i hc Quc gia  TP.HCM Người hướng dẫn khoa học : PGS.  Phản biện độc lập 1:  Phản biện độc lập 2:  Phản biện 1:  Phản biện 2:  Phản biện 3:  1 A.        lên men   ,     Saccharomyces cerevisiae.             men Saccharomyces cerevisiae .         trong ê         . u là,  ;  ; m men c ng vt ch u vang nho chom men t do. 2   Xthông s  ch thc nghi bào.  S               alginate, nm men c nh trên cellulose vi khun và nm men t    rong gel alginate   Thing vt ch trong sn xu nm men c   có 122 trang, 46 41 hình và 227    b .            chia thành hai nhóm là Saccharomyces và . Saccharomyces cerevisiae,  ,  ethanol.  là  Kloeckera, Candida, Pichia, Zygosaccharomyces,       3  Các  và Ca alginate  :   trong.     vang  , cellulose vi k có    - . Vcellulose vi               trong gia   P-mang lên   Saccharomyces cerevisiae    2 , tannin        .   - Nm men Saccharomyces cerevisiae TSV09: do phòng Vi Sinh, Bách Khoa. - Alginate: do  Sinh hc, Nha Trang. 4 - Cellulose vi khun:           Acetobacter xylinum BC12 do  Khoa. - Nho Shiraz  n. 2.2.  2.2.1.  trong gel alginate   ging (50500.10 6  2 (14%) nho Red Cardinal hiu sut c nh t bào.  hai   2.2.2.   :      m       ng (100500.10 6   (1cm x 1cm x 0,7cm4cm x 4cm x 0,7cm), pH huyn phù ging (37), t lo (100400vòng/phút), th (16h); hiu sut c nh mang.    : Ncellulose vi k -Gm mang. h trên cellulose v theo hai lên  Red Cardinal  5 -   2.2.3.   vang Lên Shiraz   trong gel alginate,     nho (200280g/L)  g          112 ppmSO 2 );  2.2.4. men    2 2%:   ;   .   ;     vang  Lên men dShiraz    2   lên men. 2.3.   s ( men) 6  : Qng kh,   2 , acid, Ca 2+ ),  Dynamic flash combustion (C,H,O,N). 2.4.  - P: ANOVA, Statgraphics plus 3.2 - : Modde 5.0  3.1.  trong gel alginate              n  : 4%, m: 100.10 6 tb/mL, n 2 : 2%, t 2 : 2h. H89,1%.   , Y 1 (%). H:  M t bào trong huyn phù ging: X 1 [50; 150]; tâm X 1 =100.10 6 tb/mL  N 2 [3; 5]; tâm X 2 =4%  2 2 2 121 88,788,383,563,94 XXXY     t.10 6  dd CaCl 2  2 : 2h.  7 tb/g. H 1 =95 %.                 alginate là 94,97±0,57%,  quy. 7               trên cellulose v m     6 tb/mL, k   cm x 1cm x 0,7cm, k          ,   pH 4, t, t . H60,2%.    2   M t bào trong huyn phù ging: X 3 [100; 300]; tâm X 3 =200.10 6 tb/mL  Th 4 [4; 6]; tâm X 4 =5h   mth 185.10 6 tb/mL; t: 4h45ph. 1,4.10 9 tb/g. H                Y 2 =62,61%.    trong gel alginate là 62,60±0,37%, quy.  Sau 2 ngày .10 8 tb mang.   3.3.    3.3.1.     8  4.10 6   gel alginate.    /   -    lag         Hình 3.10.   men vang khi  trong Hình 3.11.   lên men vang khi   [...]... ; -28 ó ó ó ó ó trong gel alginate  ng v t ch m men c m men c nh trong gel alginate và n m men t do: Hi u su t t ng h p sinh kh i tính ng lên men c a n m men c nh trên cel ó 8,0% so v i n m men t u su t t ng h p sinh kh n m men c 54 do; Hi u su t sinh t ng h p ethanol ng lên men c a n m men c 24 nh tr i n m men t so v i n m men t do, trong khi hi u su t sinh t ng h 5  so v i n m men t do â C C2 bào... 3.27 ; 7 ; - 7 ; -cellulose ) Khi so sánh v i quá trình lên men b ng n m men t do, t t c các chu k lên men b ng n m men c u có th i gian lên men ngắ th i gian lên men 101,6 78 4 ứ ắ 27 23 3 31) K gi m các chi phí 19 Hình 3.30 S quá trình lên men vang Hình 3.31 T ứ 357 T s d n t chu n chu k 7 k 33 t giá tr cao nh 34 m 7 men t T hai 5 vi c tái s d ng không làm nh 3 33) K t qu này chứng t n sinh t ng...9 P< 5 ó ó â K 3 C Saccharomyces cerevisiae TSV09 ó 3 3 200–280 g/L, c max (48h) 4 ứ nhau; men 3.3 ì 28 ử Hình 3.12 S khi ó 3 2 ó ó 10 ọ Th 9 2 K 28 T ứ 28 3 7 2 ; 35 n 49,4% õ ì ử ( ) K ứ 3 3 S ó Hình 3.14 S khi 3 3 khi hàm 3 4 ó ó R gel 2 ú – 28 ứ 11 72 ó 3.13) Nguyên nhân â , ó lên men T ó ọ K ó 3 4 do ứ K 3.3.3 ì ợ Hình 3.15 S khi men, ó 3 5 â 12 3 5 K 200g/L lên 240g/L ứ cao 25 2 T 28... ng h 5  so v i n m men t do â C C2 bào 30 – 64 m t l thoát sinh t ng h p ethanol 9 – 27% so v i gi i pháp không ngâm h t; N m men c 25 ó gel algina T T ị  S ó ọ ọ lên men vang  T ứ  Th nghi d ng n m men c quy mô pilot  ứ   Nghiên cứ c s d ng n m men c â ó o qu n n m men c ... c tái s d ng không làm nh 3 33) K t qu này chứng t n sinh t ng h p ethanol c a n m men T sinh t ng h tái s d i chu k ứ7 S 3 35) ó : ethano , si 20 Hình 3.35 T Hình 3.33 S trong quá trình lên men vang ứ 357 Khi so sánh v m men c ó ó ub ng th p Q ú T t 3.5 sử d P ươ e v â trì ị â bằ v t v t do ó : ( ườ e ) + b( d ồ m men) + e(ethanol) + fCO2 + g(glycerol) + h( d( t o qu trì ) + ( tơ vô ơ)  d u ơ) +... 0,027 2,45 46,28 6,35 4,68 0,055 0,032 0,036 (3) 23 H 46 99 46 28 45 86 â trong các quá ; 1,5% và 2,4% H C 2 85 ó T ó â ó do; n 4.1  t u m huy n phù gi ng 2 43 108.10 tb/m C C2 CaCl2 t bào trong 6 i u su t c 2 â nh là 95,0% 72 7 tb/g  6 185.10 tb/mL; th i gian c 4; k t bào trong huy n phù gi ng: nh: 4h 45phút; pH d ch huy n phù n m men: c ch t mang: 1cmx1cmx0,7cm; 40g/200mL huy n phù :2 m ; lắ o: 200... 15 ó â ó méo Hình 3.19 S ; ngâm trong dd CaCl2 â ó ứ C C2 â 7 5 99 -1 8 â 7 ; - ; - â ó â 9 2- ó ắ 3 22 K 45 ó 16 Hình 3.22 T Hình 3.23 S gel alginate T ó vang â â C C2 3,5 – 4,1 Tuy â ng h ng h p có ngâm h t ọ G â 3 23 C C2 3 M n m men c chu k 10 i n t chu k 3 24) 3 ó ắ t 5 ó 7 t 7 ó ng c a n ng c a n m me T T n 17 7( ) Hình 3.24 S 3 26) Hình 3.26 T bình ứ ứ 3 5 7 các chu 3 5 7 T 34 – 6 9– 9 ẳ 3... 90,83 90,42 89,82 88,26 98,10 97,62 e s r ( / ) 4,20 4,95 100,15 102,25 99,60 91,30 14,68 12,33 0,13 0,19 0,08 0,12 0,07 0,06 10,60 11,40 /cellulose vi b u) 91,25 91,19 99,05 5,37 101,35 100,50 13,90 0,12 0,07 0,08 10,25 T â 3 36 3 36 C T ứ â C nho và ứ â trong alginate CH1,985O0,616N0,255 CH1,985O0,616N0,255 CH1,985O0,616N0,255 CH1,985O0,616N0,255 CH0,126O0,642 CH1,952O0,664N0,122 CH0,208O0,333 CH1,985O0,616N0,255... CH0,126O0,642 CH1,952O0,664N0,122 CH0,208O0,333 CH1,985O0,616N0,255 CH0,126O1,637 CH1,952O0,664N0,122 CH0,209O0,342 CH1,985O0,616N0,255 CH1,513O1,233 CH1,952O0,664N0,122 CH0,21O0,345 Sau khi lên men 22 T ó ú â và â â 2 3 trên C6H12O6 + 0,023CH1,985O0,616N0,255 + 0,0148NH3 + 0,094H2O  0,156CH1,952O0,664N0,122 + 1,839C2H6O + 1,716CO2 + 0,111C3H8O3 + 0,009CH0,209O0,342 + 0,002C2H4O + 0,001C4H8O2 + 0,246CH0,126O1,637... - e v ) [*] ọ : glucose, fructose ó : - : NH3 - : acid tartaric, malic, citric và acetic - : ethylacetate, acetaldehyde, propanol, n-butanol, iosbutanol, iso-amyl alcohol, methanol T H2O t ươ [*], ọ â 21 men ú T ó 3 35 3.35 T T T ệ ơ t ư Glucose - fructose ư s ẩ do Ethanol CO2 Glycerol R â Acetaldehyde Ethylacetate s /alginate e sử d ( so v ư 90,83 90,42 89,82 88,26 98,10 97,62 e s r ( / ) 4,20 4,95 . so sánh vi quá trình lên men bng nm men t do, tt c các chu k lên men bng nm men c u có thi gian lên men ng. thi gian lên men  101,6 31). Nm men Saccharomyces cerevisiae TSV09: do phòng  Vi Sinh, Bách Khoa. - Alginate: do  Sinh hc, Nha Trang. 4 - Cellulose vi.              alginate, nm men c nh trên cellulose vi khun và nm men t 