hoàn thiện quy trình sản xuất và sử dụng vắc xin nhũ dầu tụ huyết trùng trâu bò p52 phòng bệnh tụ huyết trùng cho đàn trâu bò nuôi ở việt nam

8 ^ /r^ r^ A«i^^Vtfsajfr*M*»flivWS#^ (V-«eH?##.tii»V¥^Nifii^<V*N*yii^^ »-^ G6NG KGHg B() NdNG NGHISP* PIMfl £HGN TR.ONO BifeM cAP NHA Ntrd^C KCO4/06-W I Bi0 eAo f fee 10P ^^^^^^i^^^^B^^^^^ JO KHOA HOC VA CONG NGHE BO NONG NGHIEP & PINT CHirONG TRINH KHCN TRQNG DIEM CAP NBA Nir6'C KC 04/06-10 Dir AN SAN XUAT THU' NGHIEM BAO CAO TONG HOP KET QUA KHOA HQC CONG NGHE DlT AN "HOAN THIEN QUI TRINH SAN XUAT VA SU* DUNG VAC XIN NHU DAU TU HUYET TRUNG TRAU BO CHUNG P52 PHONG BENH TU HUYET TRUNG CHO DAN TRAU BO NUOI 6 VIET NAM" Ma so: KC.04 DA 07/06-10 Chu nhiem da an (kitten) chu tri du* an ^fcy ten) 6NGGIAMD6C TRAN XUAN H^NH Ban chu nUem chircmg trinh (k^ tin) ^HAMQUANGTHAI Bo Khoa hoc va C6ng nghe ^y ten) 'W^ (JgriSCKltaii PvyCJtwf TP. Ho Chi Minh, 2010 S I/O ( 2M0 CONG TY TNHH MOT THANH CONG HOA XA HOI CHU NGHIA VIET NAM VIEN THUOC THU Y TW Doc lap - Ty do - Hanh phuc' S6:JJ.S./DN-CTTY TP. HCM, ngay i^'thang ^Tnam 2010. V/v: De nghi ddnh gia kit qua de tai/d\e an a cap nha mc&c Kinh giri: - Ban chu nhi^m Chu-ffng trinh KC 04/06-10 - Van phong cac ChiroTng trinh KHCN trgng diln c3p Nha nu-d-c can cir vao Thong tu s6:12/2009/TT-BKHCN ngay 08/5 /2009 cua Bp trucmg Bo Khoa hoc va Cong nghe huang dan ve viec danh gia nghiem thu de tai khoa hoc cong nghe va du an san xuat thuc nghiem cap nha nuac, Cong ty TNHH mgl thanh vien thuoc thu y TW de nghi Ban chii nhiem Chuong trinh KC 04/06-10 va Van phong cac Chucrng trinh KHCN trong dien cap Nha nuac xem xet va to chuc danh gia cap nha nuac Icet qua thuc hien doi vai dy an sau day: Ten du an: Hoan thien quy trlah san xu^t vk su" dung \ic xin nhu dan tu huyet r^'^ng trau b6 chdng P52 ph6ng b^nh tu huyet trung cho dan trSu bd nu6i d Viet Nam. Ma s6 du an: KC 04 DA 07/06-10 Thuoc: - Chuong trinh KHCN trong diim cdp Nha nu&c "Nghien ciJu, phat trien va rfng dung c6ng ngfad sinh hoc", Ma s6: KC 04/06-10 Hop d6ng KHCN s6: 07/2008/HD - DACT-KC 04 DA 07/06-10 Thai gian thuc hien theo hop dong: tu thang 3 nam 2008 d^n thang 2 nam 2010. Thai gian dugc dieu chinh, gia han (neu c6) den: Khong Chu nhiem dir an: TS. Tran Xuan Hanh Du an tren da dugc hoi dong danh gia ca so hop ngay 13 thang 4 nam 2010, xep loai "Dat". Kem theo cong van nay la ho so danh gia cap nha nuac, gom: 1. Bao cao tong hop ket qua, bao cao torn tat va san pham KHCN cua de tai, du an vcri so luong va yeu cau theo hop dong; 2. Bao cao nhan xet ve to chiic thuc hien cua ca quan chu tri d§ tai, du an; 3. Hop d6ng va thuyet minh de tai, du an kem theo; 4. Thong tu ban hanh danh muc vac xin, ch6 phim sinh hoc, vi sinh vat. hoa chit dung trong thti y dugc phep luu hanh tai Viet Nam do Bo NN&PTNT ban hanh nam 2009; giky chung nhan dang ky san xuit thuoc thii y do Cue thu y cip nam 2G10; dSc tai lifu ve ket qua kiem nghiem vac xin tu huyet trung trau bo nhu dSu chung P52, la cac 16 san pham dugc san xudt trong du an; cac bao cao ve ket qua su dung vac xin nhia d4u tu huySt trung trau bo 6k tiem phong tai cac dia phuang; 5. Cac Bien ban kiem tra dinh ky ciia Ban chu nhiem chuong trinh, ca quan chu quan, Bg KH&CN; 6. Bao cao dk nghj quy^t toan tai chinh cua dk tai, dir an; 7. So nhat ky dir an; 8. Quyet dinh va bien ban danh gia cua Hoi dong danh gia cap ca so; 9. Van ban xac nhan ve su thoa thuan cua cac t^c gia ve vice sap xep thu tu ten trong danh sach tac gia thuc hien d6 tai, du ^n; 11. Cac tai lieu khac (n6u c6). So lugng ho so gom: - 05 bg (ban sao) diy du tai lieu ke tren; - 08 bg (ban sao) cac tai lieu tren trir tai lieu so 7 (tai lieu thiet ke, so lieu ). De nghi Ban chu nhiem Chuong trinh KC 04/06-10 va Van phong cac Chuong trinh KHCN trong dien cap Nha nuac xem xet va to chuc danh gia nghiem thu cap nha nuac ket qua d^ tai/du an./i^^:=i- THUTRU'OfNG TO CHlTC CHU TRI DlT AN '^S^^ ^AZn niAM B6C IT THANH |» I THUO'f PHAM OUANG THAI CONG TY THUOC THU Y TW CONG HOA XA HOI CHU NGHIA VIET NAM fioc lap - Ty do - Hanh phuc TP. HCM, ngay ^ thang .^" nam 2010 BAO CAO VE VIEC HOAN THIEN HO SO DANH GIA CAP CO SO I. Nh&ng thong tin chung 1. Ten du an: Hoan thi$n quy trinh sdn xuat va siJ dung vdc xin nhu dau tu huyet trung trSu b6 chiing P52 ph6ng benh tu huyet triing cho dan trSu bo nuoi d Viet Nam. Ma s6 dir an: KC 04 DA 07/06-10 Thuoc: - Chuong trinh KHCN trong dilm cip Nha nuoc "NgMen cilu, phat trien va ling dung c6ng nghe sinh hoc", Ma s6: KC 04/06-10 2. Chu nhiem du an: TS. Tran Xuan Hanh 3. Co quan chu tri du an: Cong ty thu6c thii y TW - NAVETCO 4. Chii tich hoi d6ng danh gia: PCS. TS. TrSn Dinh TCr. Hoi thu y Viet Nam. 5. Thoi gian danh gia: Bat dau: 09gia. ngay 13/4/2010 Ka thuc: 11 gia, ngay 13 M /2010 II. Noi dung da thuc hien theo ket luan cua hoi d6ng cip ca so: 1. NhiJng noi dung da bo sung hoan thien: Du an da chinh sila cAc iSi in an, chinh ta, sap xep trat tit cac noi dung trong bao cao hoan chinh theo gop y cua Hoi dong. 2. NhQng van de bo sung moi: - Bo sung nhOng hinh anh va giai thuong du an tham gia hoi chg Techmart Viet Nam Asean + 3 vao thang 9/2009. - Giai thuong TOP TEN NGANH HANG san phSm thuong hieu Viet hoi nhap WTO cho vac xin tu huyet trung trau bo nhu dau chung P52. 3. Nhung vSn de chua hoan thien duac (neu ro ly do): - IChong III. KiSn nghj ciia chii nhiem de tii/du' an: Du an da hoan thanh diy dii cac noi dung va vugt dang l<y ve so lugng san phim vac xin nhu d4u tu huyet triing trau bo chung P52. San pham vac xin da dugc sii dung tiem phong de phong benh tu huyet triing cho dan trau bo rat hieu qua tai hau het cac tinh trong nuoc dac biet cac tinh Tay Nguyen va da xuat khau. Be nghi Hoi dong nghiem thu cap nha nuoc cong nhan ket qua du an. CHU NHIEM BE TAX, DU AN (Ho, ten va chii ky) TRAN XUAN HANH XAC NHAN CUA CHU TICH HOl D6NG (HQ, ten va chit ky} XAC NHAN CUA T6 cHtrc CHU TRI DE TAI, Dir AN , chit ky va dong ddu) '^SooioSr PGS,TS.gi^J)^^ PHAM QUANG THAI BAO CAO GIAI TRINH MOT SO VAN DE TAI HQI NGHI NGHIEM THU CAP CO Sd Du- an: HOAN THIEN QUI TRINH SAN XUAT VA SU' DUNG VAC XIN NHU DAU TU HUYET TRUNG TRAU B6 CHUNG P52 PHONG BENH TV HUYET TRUNG CHO DAN TRAU BO 6 VIET NAM 1. Noi ro hem ve cai tien moi truong nuoi cay Cai tien moi trydng nuoi cay de san xuat vac xin nhu dau la van de then chot quyet dinh den thanh cong ciia dir an. Qua trinh cai tien dugc thuc hien bang each bo xung mot so cac yeu to dinh duong can thiet nham nang cao sir phat trien ciia vi khuan P.multocida trong dieu kien nuoi cay trong Fermenter voi cac thong so ve pH, toe do khuay dao, do thong Idii (do hoa tan oxy) da dugc chuan hoa. Viec cai tien moi truong thuc hien trong du an nay da dam bao dugc 3 van de: Tang hieu qua nuoi cay ciia vi khuan P.multocida, chiing P52 Moi trirong de che tao va de kiem soat ve mat chat lugng Khong CO sir bien dong ve gia thanh san xuat moi tnrong Thirc te cho thay nong do vi khuan thu hoach dugc tang len khi nuoi cay trong moi truong cai tien khong nhitng dam bao du tieu chuan de san xuat vac xin nhu dau, ma con mang y nghia kinh te rat Ion. Vi day la mot du an san xuat thu nghiem, san pham la vac xin doi hoi ky thuat cao va dieu kien nghiem ngat khi san xuat, hon nira trong dieu kien kinh te thi truong, sir canh tranh giiia cac nha san xuat rat khac nghiet, do vay viec giii bi mat cong thiic, cac bi quyet la van de can dirge ton trong, vi vay chiing toi xin phep khong neu chi tiet cong thirc, thanh phan moi trucmg cung nhu cong thitc phoi hgp lam nhii dau. Tuy nhien chiing toi khang djnh rang chat lugng vac xin san xuat tir moi truong cai tien dat chit lugng tot va dieu nay da dugc chiing minh qua 16 16 vac xin voi hon 7 trieu lieu vac xin da san xuat deu dat tieu chuan chat lugng va khi sir dung trong thuc te cho kit qua an toan va hieu qua bao ho cao. 2. Cach che khang nguyen diing trong ky thuat ELISA phat hien Ichang the THT trau bo? Tac gia c6 the dong y chuyen giao ley thuat ELISA? Ky thuat ELISA dliu tien dugc chung toi phat trien de sir dung phat hien khang the tu huyet triing heo (Septicaemic Pasteurellosis) vgi khang nguyen diing chua vi khuan Pasteurella multocida serotype B:2. Ky thuat nay dugc thirc hien tai khoa thu y (Vet School) truong dai hgc Queensland, Australia duoi su huang dan cua giao su Alan Frost. Tir ket qua nay, chung toi da chuan hoa de dung kiem tra Idiang the benh tu huyet triing trau bo (Haemorrhagic septicaemia). Khang nguyen diing trong ky thuat nay la khang nguyen chiu nhiet, diing chung VP 816 (hoac) HPKT99. Day la chung dugc phan lap tir mot 6 djch tu huyet triing trau bo va heo tai tinh Kontum vao nam 1999 va bang cac phuong phap khac nhau (huyet thanh hoc, PCR) da xac dinh la vi khuan P. multocida serotype B:2 (phuong phap che tao khang nguyen tham khao trong luan an tien sT ciia Tran Xuan Hanh, 2002). Ve mat chuyen giao ky thuat: Day la mot phuang phap danh gia mien dich trong benh tu huyet trung rat chinh xac va hieu qua ve mat kinh te, do vay chung toi san sang chuyen giao ky thuat neu dugc yeu cau. 3. Loi chinh ta va each trinh bay trong bao cao. Chiing toi da kiem tra lai mot each can than, sira loi chinh ta, cac loi in an va trinh bay lai mot so bang bieu cho de theo doi hem theo nhu gop y ciia hoi dong. Chu nhiem du an TS. Tran Xuan Hanh CONG TY TNHH MOT THANH VIEN THUOC THU Y TW CONG HOA XA HOI CHU NGHIA VIE I NAM Doc lap - Ty do - Hanh phiic TP. HCM, ngay Jc thang ,-? nam 2010 DANH SACH TAC GIA THVC HIEN DE TAI KHCN/Dir AN SXTN CAP NHA NlTdC (Danh sach nhitng ca nhan da dong gop sang tao chii yeu cho de tai, du an ditac sap xep theo thit tu do thoa thuan) 1. Ten dir an: Hoan thien quy tiinh sJn xuS't v4 sil dung vdc xin nhu dau tu huyg't triing trau bo chiing P52 ph6ng bfinh tu huyet triing cho dan ttau bo nuoi S Vi$t Nam. Ma s6 du an: KC 04 DA 07/06-10 Thuoc: Chuong trinh KHCN trong diem cdp Nha nuoc "Nghien clJu, ph£t tri^n v4 iJng dung cong ngh^ sinh hoc". Ma s6: KC 04/06-10 2. Thai gian thirc hien: tir thang 3 nam 2008 dSn thang 2 nam 2010 3. T6 chirc chii tri: Cong ty TNHH mot thanh vien thudc thii y TW-NAVETCO. 4. Co quan chu quan: Bo Nong nghiep va PTNT. 5. Tac gia thuc hien de tai/dir an tren gom nhimg nguoi c6 ten trong danh sach sau So TT 1 2 3 4 5 6 7 8 9 Ch^c danh khoa hoc, hoc vi, ho va ten TS. Tran Xuan Hanh TS. Nguyen Thien Thu TS. Pham Quang Thai BS. To Thi Phan BS. VoCongMmh ThS. D6 Vanfiiang BS. Pham Hao Quang ThS. Nguyen Van Dung KS. Nguyen Tang Kien To chirc cong tac Cong ty thuoc thu y TW Cong ty thuoc thii y TW C6ng ty thuoc thu y TW - Cong ty thuoc thii y.TW Cong ty thuoc thii y TW Cong ty thuoc thii y TW • Cong ty thuoc thii y TW Cong ty thuoc thii y TW Cong ty thuoc thu y TW ChCtky 1.)^''^- 'Wfz^^^' /^t^£ -^ ^y^- •f'tfii- V> Chu nhifm de tai/dy an (Ho, ten vd chu ky) Thu truong to chiic chu tri de tai/du* an ', chir ky vd dong ddu) ^•si^rrS^^ynMO GIAM DOC PHAM QUANG THAJ CONG TY TNHH MOT THANH VIEN CONG HOA XA HOI CHU NGHIA VIET NAM THUOC THi) Y TW Doc lap - Tir do - Hanh phiic TP. HCM. ngay 9 llidng 4 nam 2010 NHAN XET VE TO CHlTC THtfC HIEN DE TAI KHCN/Dir AN SXTN CAP NHA NlTOfC 1. Thong tin chung: 1 Ten dir an: Ho&n thien quy trinh san xurft va si dung vac xin nhu diu tu huy^t triing trSu b6 chiing P52 ph6ng bfinh tu huy^t triing cho dJn trau bo nuoi d Vi6t Nam Ma s8 dif an: KC 04 DA 07/06-10 Thuoc: Chuong trinh KHCN trong diim cap nha nuoc "Nghi8n ci?u, ph^t trien va itng dung cong nghe sinh hoc", Ma s6: KC 04/06-10 2. Ten chu nhiem: TS. Tran Xuan Hanh 3. Ten to chirc chii tri: Cong ty TNHH mot thanh vien thuoc thu y TW- NAVETCO 4. Tong kinh phi thuc hien: 6.695,876 trieu d6ng - Trong do, Kinh phi tir ngan sach SNKH: 2.000 tr.d - Ty le va kinh phi thu h6i (ddi v&i Die an SXTN): 60% bSng 1.200 Tr. d. 5. Thoi gian thirc hien: 24 thang - Bat dau (theo hgp dong): thang 3 nam 2008 - KSt thuc (theo hgp d6ng): thang 2 nam 2010 - Dirge dieu chinh, gia han (niu c6) den: Khong 6. Tai lieu kem theo (neu c6): (van ban gia han, ket luan cua thanh tra, kiem tra ) Dir an khong phai gia han II. Nhan xet vl t6 chirc thirc hien: 1. Nhan xet vk Ti6n do thirc hien: Thuc hien dung han: Dir an trien khai thuc hien dung han. BChSng thuc hien diing han (neu ro ly do ): Khong. 2. Nhan xet v6 tinh hinh sil dung va huy dong nguon kinh phi thuc hien. [...]... le tiem phong uoc tinh dat khoang 50% (4 trieu con) chi phi cho viec tiem phong vac xin cho moi loai vac xin trong 1 nam se la: -I- Vac xin keo phen: 4 (trieu con) x 6.600 (dong) = 26,4 ty d6ng /nam -I- Vac xin nhii dau (tiem 1 Ian): 4 trieu x 3424 (d) = 13,7 ty dong /nam -I- Vac xin nhii dau (tiem 1,5 lin): 4 trieu x 5136 (d) = 20,5 ty d6ng /nam 3 Tinh hinh thirc hien che do bao cao, kilm tra cua de... nghe san xuat vac xin THT trau bo nhu dau chiing P52 6 qui mo cong nghiep Hoan thien qui trinh ki^m nghiem vac xin THT trau bo nhu dSu chimg P52 Xay dung phuong phap danh gia mien dich va bao h6 ciia vac xin trong di^u ki6n thuc te' bang ky thuat ELISA Xay dirng qui trinh bio quan, van chuyen va sir dung vac xin Danh gia so sanh hieu qua phong benh ciia vac xin nhu P52 vol cac vac xin truyen thong khac... xin vo hoat tu huyet trung trau bo nhu dau chiing P52 (qui mo cong nghiep) 2 Qui trinh kiem nghiem vac xin vo boat tu huyet trimg trau bo nhu dau chiing P52 3 Qui trinh ky thuat ELISA dung phat hien khang the Pasteurella multocida serotype B:2 4 Qui trinh bao quan vac xin vo hoat tu huyet trung trau bo nhu dau chiing P52 5 Qui trinh van chuyen vac xin vo hoat tu huyet trung trau bo nhu dau chiing P52. .. san xuat 7 trieu lieu vac xin THT trau bo nhu dau chiing P52 cung cap cho thi truong trong nuac va xuat khau Ap dung de kiem nghiem vac xin THT trau bo nhu dau chiing P52 i truac khi xuat xuong Duac ap dung de danh gia mien dich ciia trau bo duac tiem vac xin THT trau bo Ap dung tai kho vac xin ciia Cong ty va tai cac chi cue thii y Ap dung tai Cong ty i Ichi van chuyen vac xin tai cac chi cue thii... thgi gian va nhan lire + Tang ty le tiem phong va ty le bao ho cho dan trau bo + Nang cao hieu qua kinh te cho nguoi chan nuoi Chi phi t6 chirc tiem phong cho 1 dau trau bo/l nam dugc so sanh: Chi so Vac xin GiaVX (d6ng) Lan tiem /nam Chi phi tiem phong/lan (d6ng) Chi phi/con/ nam (d6ng) Ghi chii keo phen 1.300 2 2.000 6.600 1 2,000 3.424 Vac xin nhu dau Viing it de doa 1.424 Viing CO 1,5 2.000 5.136 nguy... nghiep vac xin nhu dau Chuan hoa qui trinh bao quan van chuyen, sir dung vac xin nhil Danh gia so sanh VX nhii v6i cac loai vac xin keo phen khac Xay dung qui trinh kiem nghiem vac xin Xac dinh do dai mien dich cua vac xin HoEln thien qui ttinh ki^m nghi§m cac chi tieu vacxin bang phUPng phap thay thS' va trong tai Phu trach tri^n khai San xuat duac san xu3't vac xin qui hon 7 trieu lieu vac xin nhu dau... cua vac xin THT trau bo keo phen, vac xin hien nay dang dugc sir dung phong benh THT trau bo a nuac ta, trong chuong trinh cai tien nang cao chat lugng cua vac xin THT, Trung tam nghien cim thii y thuoc Cong ty thuoc thii y TW-NAVETCO, da nghien ciiu cai tien chat bo trg mien dich ciia vac xin THT trau bo keo phen chimg P52 thanh vac xin THT trau bo nhii dau (vac xin THT trau bo keo phen chiing P52 da... vac xin THT trau bo nhii dau chimg P52 qui mo cong nghiep; ii/ Hoan thien qui trinh kiem nghiem vac xin THT nhii dau; iii/ Hoan thien qui trinh bao quan, sir dung, van chuyen vac xin THT trau bo nliQ dau; iv/ So sanh hieu qua ciia vac xin THT trau bo nhii dau chiing P52 voi cac vac xin THT khac; v/ Xay dung phuong phap kiem tra mien djch de danh gia miln dich ciia dan gia site dugc tiem phong vac xin. .. tinh In djnh phugng phap ly tam va dg nhat t phuang phap cho chay tu do (the phuang phap kiem nghiem cua T Qu6c) Ket qua cho thiy vac xin tieu chuan ve vat ly Kiem tra v6 triing Kiem tra chat lugng vac xin tu huyet trimg trau bo nhij dau chung P52 Tinh chit vat ly ciia vac xin Hoan thien qui trinh kilm nghiem vac xin THT trau bo nhu dfiu chung P52 ... cong nghe: Vac xin dugc san xuat dat cong nghe tien tien dugc ap dung o nhieu nuoc tren thi gioi b) Hieu qua ve kinh te xa hoi: Do nhijng iru viet ciia vac xin nhQ dau tu huyet tning trau bo chiing P52 da dugc nghien cuu trong du an, loi ich ciia vac xin the hien cii the nhir sau: + Giam so Ian tiem phong tit 1 nam 2 Ian xuong 1 nam 1 Ian (hoac 1,5 lin) + Giam chi phi to chirc tiem phong cho cac dja phuong . an: Hoan thien quy trlah san xu^t vk su" dung ic xin nhu dan tu huyet r^'^ng trau b6 chdng P52 ph6ng b^nh tu huyet trung cho dan trSu bd nu6i d Viet Nam. Ma s6 du. du an: Hoan thi$n quy trinh sdn xuat va siJ dung vdc xin nhu dau tu huyet trung trSu b6 chiing P52 ph6ng benh tu huyet triing cho dan trSu bo nuoi d Viet Nam. Ma s6 dir an:. Hoan thien quy tiinh sJn xuS't v4 sil dung vdc xin nhu dau tu huyg't triing trau bo chiing P52 ph6ng bfinh tu huyet triing cho dan ttau bo nuoi S Vi$t Nam. Ma s6