Human DNA repair enzyme o6 methylguanine DNA methyltransferase in cellular regulation upon DNA damage

... change in human DNA repair enzyme O6- methylguanine -DNA methyltransferase upon alkylation of its active site by SN1 (indirect-acting) and SN2 (direct-acting) alkylating agents: breaking a "salt-link" ... bromodomain and the third zinc finger SRC interacts at a SID (SRC interacting domain for binding SRC) domain and PCAF interacts at the CH3 domain Interestingly, p300 was re...

Ngày tải lên: 16/09/2015, 12:15

... reported that 5% of the cases of oral squamous cell carcinoma show diminished expression of hMSH2 protein In the present study, we demonstrated decreased expression of hMSH2 protein in reticular ... OLP subtypes Figure Positive labelling for hMSH2 protein in basal and intermediate epithelial layers of reticular subtype of oral lichen planus patient (str...

Ngày tải lên: 03/11/2012, 09:54

... characterization of Pol k from a higher plant, rice, using cDNA and recombinant proteins The results suggest that plant DNA Pol k is a DNA repair enzyme which functions in plant meristematic and ... S., Kasai, N., Shimazaki, N., Takemura, M., Asahara, H., Linn, S., Yshida, S., Matsukage, A. , Koiwai, O., Sugawara, F., Yoshida, H & Sakaguchi, K (2002) A plant...

Ngày tải lên: 07/03/2014, 15:20

... 2A) The N-terminal part of XPCB hHR23B B C Fig NMR structure of the XPCB domain of hHR23B (A) Stereoview of the 12 superimposed structures of XPCB hHR23B All Pro residues conserved among the ... further our knowledge with respect to the mechanisms of action of these proteins, it is crucial that we define the precise boundaries of the STI1-homologous domai...

Ngày tải lên: 07/03/2014, 17:20

... proteins specialized in DNA repair Interestingly, they also contain motifs typical of proteins involved in RNA metabolism TgDRE mRNA and protein expression have been evaluated using RT-PCR and ... many new avenues of future investigations into the unexplored molecular mechanisms involved in DNA repair and RNA metabolism in the protozoan parasites such as T g...

Ngày tải lên: 08/03/2014, 22:20

... unwavering support of my goals iv ABSTRACT Sara M McNeil ELUCIDATING THE ROLE OF REDOX EFFECTS AND THE KU8 0 CTERMINAL REGION IN THE REGULATION OF THE HUMAN DNA REPAIR PROTEIN KU DNA double strand breaks ... than that of the 16 kDa Ku8 0CTR band and the band intensity generated from Ku7 0 and Ku8 0 C antibodies, indicating a small per...

Ngày tải lên: 24/08/2014, 11:02

... Anatomy of a DNA replication fork revealed by reconstitution of SV40 DNA replication in vitro Nature 369, 207–212 Tsurimoto T & Stillman B (1991) Replication factors required for SV40 DNA replication ... al ¨ Human DNA polymerases a, d and e in replication A B Fig Effect of inhibitory antibodies on replicative DNA synthesis in isolated, permeabilized nuclei duri...

Ngày tải lên: 30/03/2014, 10:20

... design DNAdirected alkylating agents by linking the alkylating pharmacophore to the DNA- affinity molecules (e.g., DNA intercalating agents, DNA minor groove binder) [7,8] In most cases, the DNA- directed ... article as: Chu et al.: Enhancement of radiosensitivity in human glioblastoma cells by the DNA N-mustard alkylating agent BO-1051 through...

Ngày tải lên: 09/08/2014, 09:20

... doi:10.1186/gm145 Cite this article as: Dalgleish R, et al.: Locus Reference Genomic sequences: an improved basis for describing human DNA variants Genome Medicine 2010, 2:24 ... at EBI to formulate the specification of an improved reference sequence that is now known as Locus Reference Genomic (LRG) Creation of the formal LRG specification began in May 2008 an...

Ngày tải lên: 11/08/2014, 12:20

... observation that Ku binds to retroviral preintegration complex (PIC) raises the possibility that DSB repair enzymes may play other roles in integration or pre- We report here that defects in DSB repair ... Modeling the late steps in HIV-1 retroviral integrase-catalyzed DNA integration J Biol Chem 2000, 275:39287-39295 Yoder KE, Bushman FD: Repair of gaps in retroviral...

Ngày tải lên: 12/08/2014, 23:22

... Contamination of human DNA samples with mouse DNA can lead to false detection of XMRV-like sequences Retrovirology 2010 7:109 Submit your next manuscript to BioMed Central and take full advantage of: • ... obtained with the XMRV gag PCR assay are due to variable contamination of the human samples with mouse DNA, most likely in laboratory reagents...

Ngày tải lên: 13/08/2014, 01:20

... number of repair enzymes is increased the efficiency of the charge exchange mechanism decreases In this way, doubling the number of repair enzymes only shortens the location time by 30%, not by 50% ... is the N/NMutY repair enzyme to arrive at the damaged site Thus the MutY location time MutY tlocation = N N MutY (4) tlocation Here N is the total number of repair enz...

Ngày tải lên: 13/08/2014, 22:22

... and spatially controlled, how Checkpoint and Coordinated Cellular Responses to DNA Damage 83 checkpoint signaling is regulated by different types of DNA damage, and how different downstream cellular ... after DNA damage might expose these histone modifications to present a damage- specific “histone code” to proteins involved in DNA repair or checkpoint si...

Ngày tải lên: 25/10/2013, 21:20

... hypoxia and reoxygenation have been reported to induce DNA damage [30–32], we examined the effects of pyruvate addition during hypoxia and after reoxygenation on DNA damage HepG2 cells were incubated ... by Singh et al [48], using alkaline electrophoresis, which allows detection of single-strand and double-strand breaks Pyruvate reduces DNA damage during...

Ngày tải lên: 18/02/2014, 16:20