dna damage checkpoint signaling pathways in human cancer

... held hour at 4°C and incubated at 37°C for 24 hours The study of initial DNA damage was completed in the University of Granada (Spain) Initial radiation-induced DNA damage in PBLs was measured ... ln(Gy), confirming our previously observations [13,17,19] The increments in RIA were defined by two constants: the coefficient in origin a (as the origin of the curve in the Y axis determining the spontaneous ... to restore DNA sequence integrity remains unclear Intrinsic radiosensitivity is correlated in a first approach to the ability of the cell to detect and repair DNA damages DSB can be induced by...

Ngày tải lên: 09/08/2014, 09:20

... quadruplex-binding acridine ligands BRACO-19 and RHPS4 (Fig 1), in common with telomestatin, induce rapid replicative senescence in cancer cells and activate the same DNA damage response that follows DNA ... A431 human epithelial carcinoma MDA-MB-231 human breast cancer MIA PaCa-2 human pancreatic cancer UXF1138L human uterine carcinoma M14, LP, LM melanoma RHPS4b CG5 breast carcinoma Quarfloxin Quarfloxin ... breaks This involves in particular ATM, p16INK4a kinase and p53 pathways [32–35] which can be visualized by the appearance of charac- teristic DNA damage foci using an antibody to the damage response...

Ngày tải lên: 06/03/2014, 09:22

... calmodulin and PtdIns3K In guinea pig neutrophils, it has been demonstrated that inhibition of calmodulin results in inhibition of Rac and p21 activated kinases [50] Involvement of Rac in ROS ... Ca2+/calmodulin-dependent kinases in human neutrophils Increased [Ca2+]i is sufficient to regulate multiple intracellular signaling pathways Therefore, the role of calmodulin, the major downstream effector of Ca2+, in ... calmodulin Additionally, these data suggest that fMLP induced GSK-3a phosphorylation in human neutrophils can occur in a PKBindependent manner Binding of Ca2+ to calmodulin enables it to interact...

Ngày tải lên: 08/03/2014, 10:20

... f, (inset in i), m, (inset in n), o, (inset in o), p, (inset in r)) 20 µm; (e, g, h, i, q, r) 50 µm Local injury to the hippocampus via saline injection produces a strong induction of activin-βA ... compared to the uninjured brain 15 16 Trends in Cell Signaling Pathways in Neuronal Fate Decision A major component of the brain post-injury in comparison to the uninjured brain is the inflammatory ... highlighted in the following sections Biological effects associated with Insulin/IGF-signalling in neural development 3.1 Insulin/IGF-signalling in ESC Insulin and IGF are important factors to keep human...

Ngày tải lên: 16/03/2014, 20:21

... Ras-RafMEK-ERK signaling during RV infection in RK13 cells The PI3K inhibitor LY294002 and the MEK inhibitor U0126 were used to investigate PI3K-Akt and Ras-Raf-MEK-ERK signaling respectively during RV ... http://www.virologyj.com/content/2/1/1 Inhibition of PI3K results in an increase in the speed and magnitude of RV-induced apoptosis To evaluate the role of PI3K-dependent signaling during RV infection, the effects of PI3K inhibitor ... Figure Kinase phosphorylation during RV infection Kinase phosphorylation during RV infection Serum-starved RK13 cells were mock infected or infected with RV at an m.o.i of PFU/cell At indicated...

Ngày tải lên: 18/06/2014, 22:20

... required for the DNA damage response in vivo in C elegans and in vitro in human breast cancer cells Oncogene 2009, 28:2419-2424 12 Maes OC, An J, Sarojini H, Wu HL, Wang E: Changes in MicroRNA Expression ... dissemination and migration of malignant cells during a course of radiation therapy, since miR151-5p was found to increase migration and intra-hepatic metastasis in hepatocellular carcinoma [15] Indeed, ... exerts its effects, involves changes in the tumor microenvironment Interestingly, miR-24-1 levels were increased following irradiation and miR-24-1 might influence angiogenesis, invasion and local...

Ngày tải lên: 09/08/2014, 09:20

... Combination of FWGE with 5-FU, Oxaliplatin and Irinotecan in human colon cancer cell lines The combined drug effect of a parallel exposure to FWGE and either 5-FU, irinotecan or oxaliplatin was ... observed for the combinations of FWGE and 5-FU (6 out of cell lines) and to a lesser extend for irinotecan and oxaliplatin (2 out of cell lines) Drug interaction for the remaining cell lines was additive ... drug interaction of FWGE with irinotecan, oxaliplatin and 5-FU These data are in line with a previous clinical report of Jakab et al They observed in their study with colon cancer patients an increased...

Ngày tải lên: 10/08/2014, 10:21

... (FRET)/emission ratioing using a classic laser point scanning microscope, this is associated with a number of challenging technical issues (see introduction) In these studies we therefore used a spinning disk ... b2-adrenoceptors in human primary cells [28] In their review, Evans et al., [22] highlighted the importance of confirming “proof of principle” studies of ligand-directed signaling in recombinant systems ... agonists determined via confocal emission ratio imaging In addition to isoproterenol, we assessed the fold increase in confocal emission ratio imaging in response to a range of clinically relevant...

Ngày tải lên: 12/08/2014, 13:22

... human KDR expression [11], while TGF-β inhibits Flk-1/KDR expression through a mechanism that involves reduced binding of GATA-2 to a palindromic GATA site in the 5'-UTR [30] These findings indicate ... constructs containing different deletion segments of mouse Flk-1 promoter sequence [22], including binding sites for E-Box, Sp1, AP2 and NF-κB (Fig 5A) As shown in Fig 5B and 5C, deletion binding sites ... Flk-1 expression in mouse lungs HIMF enhances Flk-1 expression in mouse lungs Recombinant HIMF protein or BSA was intratracheally instilled into adult mice (200 ng/animal in 40 μl saline, n = for...

Ngày tải lên: 12/08/2014, 16:20

... determine inhibition of viral binding (■), or co-cultured with permissive T cells (PM-1) to determine inhibition of viral transfer ( ) Background binding to Raji cells was determined using Raji/DC-SIGN- ... by steric hindrance following binding to CD4 as first thought [9,14], but through bind- Figure Inhibitory effect of polyanionic compounds against HSV-2 infection of epithelial cells Inhibitory ... concentrations in the above models, we then investigated the potential of either compound to elicit pro-inflammatory cytokine production in human cervical tissue Only increases in IL-1β and RANTES, following...

Ngày tải lên: 13/08/2014, 09:20

... Besides stimulating proliferation, serum exposure induces a wound healing response in fibroblasts, involving the activation of genes that play a role in intercellular signaling and remodeling of the ... [22] In cancer, chemokine signaling can also affect migratory behavior For instance, mesenchymal stem cells in the tumor stroma are able to increase breast cancer cell motility through paracrine ... the increased metabolic activity of cancer cells, and chromatin remodeling (ACTL6A, SMARCC1), indicating that epigenetic mechanisms may be involved in the maintenance of an embryonic phenotype in...

Ngày tải lên: 14/08/2014, 20:22

... performed by heating sections in pH 6.0 citrate buffer Sections were then rinsed in PBS and incubated in blocking buffer (5% bovine serum albumin, 0.3% Triton X-100, 10% normal goat serum, in PBS) for ... between multiple signaling pathways, the ability to detect the activation status of individual pathways within a complex network of other pathways in the cell is of paramount importance In this manner, ... principal component (PC1) MTB/ TRAS mice uninduced (open triangles) or induced (filled triangles) for 48 or 96 h were used for training, while the remaining MTB/TRAS time points and MTB uninduced...

Ngày tải lên: 14/08/2014, 21:20

... eIF4E binding protein beclin-1-regulated autophagy AMP activated protein kinase artesunate bafilomycin A1 Bax-interacting factor big mitogen-activated protein kinase bovine serum albumin cycloheximide ... regulatory-associated protein of mTOR rapamycin-insensitive companion of mTOR reactive oxygen species Beclin-1 interacting and cystein-rich containing S6 kinase Small interfering RNA soluble N-ethylmaleimide-sensitive ... 2000; Sinha et al., 2013) MAPK signaling pathways including ERK, JNK, p38 kinase, ERK3/4, and BMK1 (big mitogen-activated protein kinase 1) pathway have been identified as important signaling pathways...

Ngày tải lên: 09/09/2015, 08:16

... 1.2 Incidence of cancers in Singapore Both tables were taken from the Singapore Cancer Registry Interim Report 13 Section Introduction Figure 1.6 Figure 1.6 Ten most prevalent cancers in Singapore ... and perhaps involve in shaping human diseases including malignancies Hence, it is important to systematically identify low abundance genes that are altered in human disease Section Introduction ... according to projections carried out by WHO in year 2006 12 Section Introduction Table 1.2a Incidence of cancers by gender in five-year period Singapore, 1968-2002 Table 1.2b Incidence of cancers...

Ngày tải lên: 12/09/2015, 09:59

... BRCT-domain BRCT-domain BRCT-domain PI3K-like ATR-binding PI3K-like Kinase Kinase Nuclease Transcription factor Phosphatase APC-inhibitor Kinase Plk1-4 Table DNA damage checkpoint proteins DNA damage ... employ checkpoints that monitor various cellular events [58] Four major checkpoints are now known in the budding yeast: morphogenetic checkpoint, DNA replication checkpoint, DNA damage checkpoint ... the DNA damage checkpoint 1.1.2 DNA damage checkpoint (DDC) As expected, the DNA damage checkpoint pathway and its components are wellconserved across eukaryotes (see Table 1) The checkpoint...

Ngày tải lên: 14/09/2015, 08:23

... JAB Janus kinase binding protein JAKs Janus protein-tyrosine kinases JNK c-Jun N-terminal kinases MAPK mitogen activated protein kinase xiv MAPKK mitogen activated protein kinase kinase MAPKKK ... hydrogen peroxide HIV human immunodeficiency virus IAPs inhibitor of apoptosis proteins IL Interleukin Iκ B NF-κB inhibitory protein IKK IκB kinase INF-γ interferon-γ iNOS inducible nitric oxide ... resistance to doxorubicin-induced cytotoxicity in cancer cells 4.3.5 Andro enhances doxorubicin-induced apoptosis in human cancer cells 123 127 4.3.6 Andro sensitizes doxorubicin-induced apoptosis...

Ngày tải lên: 14/09/2015, 14:11

... as a new binding partner for integrinlinked kinase (ILK), which is also an adaptor within integrin-associated cytoskeletal complexes implicated in integrin-mediated signaling (Mackinnon et al., ... consequently integrins in mammalian cells (Tu et al., 2003) 1.3.2 Integrin signaling pathway in cell growth control Integrins are cell surface receptors connecting cells to the surrounding ECM They ... in patients with Kindler syndrome, an autosomal recessive skin disorder They therefore defined a family of kindlin-related proteins namely Kindlin-1, Kindlin-2 (mig-2) and Kindlin-3 (Siegel et...

Ngày tải lên: 05/10/2015, 22:15

... cisplatin -DNA damage involves the induction of DNA repair proteins to initiate DNA repair [10] We determined if NaAsO2 and hyperthermia modulated the expression of XPC, a platinum -DNA damage ... (A2780/CP70) human ovarian cancer cells were the kind gift of Dr Eddie Reed Cells were maintained in RPMI 1640 medium containing 10% fetal bovine serum, 100 μg/mL penicillin/streptomycin, mM L-glutamine ... suppressing MSH2 Sodium arsenite alone or combined with hyperthermia inhibits mechanisms of cisplatin resistance by suppressing XPC induction, maintaining higher levels of MSH2 and increasing tumor...

Ngày tải lên: 20/06/2014, 07:20