dna damage response and dna repair

... 87(15):5837–5841 18 DNA Repair − On the Pathways to Fixing DNA Damage and Errors Holmes, AM & Haber, J (1999) Double-strand break repair in yeast requires both leading and lagging strand DNA polymerases, ... Sgs1 helicase and two nucleases Dna2 and Exo1 resect DNA double-strand break ends, Cell 134(6):981-994 2 Synergy Between DNA Replication and Repair Mechanisms Maria Zannis-Hadjopoulos and Emmanouil ... essential role in several DNA repair pathways, such as base excision repair, nucleotide excision repair, and double-strand (ds) break and mismatch repair Recent work from prokaryotes and eukaryotes has...

Ngày tải lên: 28/06/2014, 05:20

... phosphorylation, and kinetics of repair of DNA strand breaks in irradiated cervical cancer cell lines Cancer Res 2004, 64(19):7144-7149 47 Bilsland E, Downs JA: Tails of histones in DNA double-strand break repair ... double-strand DNA damage and complex with other important repair molecules g-H2AX is required for foci formation for numerous factors including p53, MRN complex (MRE-11, RAD50, and NBS1), and BRCA1 ... produce DNA interstrand and/ or intrastrand cross-links [29,30] As has been known, bifunctional alkylating agents induce collapsed replication forks that can lead to either cell cycle arrest, DNA repair, ...

Ngày tải lên: 09/08/2014, 09:20

... neighborhood network restricted to DNA damage repair response The 3-core representation of the G2* second neighborhood network restricted to DNA damage repair response Shown is the result of clustering ... analysis to the DNA damage repair response Thus, we asked which of these known protein interactions has a known function that would potentially impact the cellular DNA repair response process ... which DNA- PKcs is a member (DNA- PKcs, PCNA, PARP, Ku70, Ku86, and TRBP) Thus, DNA- PKcs demonstrates an increased rank when consideration is refocused toward protein interactions involved in DNA damage...

Ngày tải lên: 13/08/2014, 05:21

... BER but also in DNA doublestrand break repair and meiosis, and Pol b and TdT may be evolved for differentiation and development of specific organs in vertebrates such as the nervous and immune systems ... meristem and panicle (lanes and in Fig 5A), and showed Plant DNA polymerase k (Eur J Biochem 271) 2805 moderate expression in the roots, root apices, flag leaves and young leaves (lanes 3, 4, and 7) ... involved in DNA repair and cell cycle checkpoint regulation on DNA damage [13] shows a lower degree of homology to human Pol k To determine the phylogenetic relationship between OsPol k and other...

Ngày tải lên: 07/03/2014, 15:20

... with orientations that differ between hHR23B and hHR23A are shown Also, b-N and b-C are the N- and C-termini of XPCB–hHR23B; a-N and a-C are the N- and C-termini of hHR23A FEBS Journal 272 (2005) ... (HT1 and HT2) were identified in XPCB–hHR23B, one between helix and helix 3, and one between helix and helix 4–5 These two trails were linked by a hydrophobic linker patch between helix and helix ... inducible) and TPR (tetratrico peptide repeat) domains (B) Multiple sequence alignments of the XPCB domains of hHR23B and hHR23A with other STI1-homologous domains from yeast STI1 and DSK2, and from...

Ngày tải lên: 07/03/2014, 17:20

... involved in DNA repair and RNA metabolism in the protozoan parasites such as T gondii and the Plasmodia Further analyses should contribute to our understanding of the biochemistry of DNA repair proteins ... I & Tinland, B (2002) Repair of damaged DNA by Arabidopsis cell extract Plant Cell 14, 263–273 Cromie, G.A., Connelly, J.C & Leach, D.R (2001) Recombination at double-strand breaks and DNA ends: ... P.C (1999) A phylogenomic study of DNA repair genes, proteins, and processes Mut Res 435, 171– 213 Marti, T.M., Kunz, C & Fleck, O (2002) DNA mismatch repair and mutation avoidance pathways J...

Ngày tải lên: 08/03/2014, 22:20

... ATM, ATR and DNA- PKcs Role of Nbs1 in DNA damage checkpoint control The similar clinical phenotypes of AT, NBS and ATLD suggested that the MRN complex functions in the same DNA damage response ... kinase, ATR, and the catalytic subunit of DNA- dependent protein kinase (DNA- PKcs), respectively This conserved motif found in Nbs1, ATRIP and Ku80 thus appears to be important for DNA damage responses ... recruitment of ATM to the region of DNA strand breakage and in thr activation of ATM in a manner dependent on DNA strand breaks Whether ATM directly recognizes or senses DNA strand breaks, or whether it...

Ngày tải lên: 16/03/2014, 13:20

... Labeling DNA Damage with Terminal Transferase I LABELING DNA BREAKS USING TERMINAL TRANSFERASE (TUNEL ASSAY) Walker et al Labeling DNA Damage with Terminal Transferase Labeling DNA Damage with ... single stranded DNA molecules containing a 3'-OH and will attach nucleotides to a single-strand nick in DNA This is particularly useful since many single-strand breaks are also introduced into DNA ... study of DNA damage holds a wide interest within both basic and applied fields of research Elucidating the mechanisms involved in the generation of DNA damage, and the consequences of this damage, ...

Ngày tải lên: 11/04/2014, 09:47

... [14-16] Oxaliplatin and cisplatin share the similar mechanism, and cause mono-adducts and intra-strand or inter-strand cross-links in the double DNA helix that severely block DNA synthesis [17-19] ... pathways of DNA damage repair may switch on, including nucleotide excision repair (NER), mismatch repair (MR), homologous recombination (HR), translesion DNA synthesis (TLS) [20] These adduct repairs ... indicator for poor treatment response and shorter survival in patients of above settings DNA polymeraseh is coded by POLH gene which is one of the 150 human DNA repair genes, whose defection...

Ngày tải lên: 18/06/2014, 16:20

... bulky DNA damage that is repaired mostly by the nucleotide excision repair system (NER) Cellular response to cisplatin -DNA damage involves the induction of DNA repair proteins to initiate DNA repair ... Following DNA damage recognition, downstream DNA repair proteins (XPA, RPA, TFIIH complex, ERCC1/XPF and XPG) are recruited to the DNA damage recognition complexes in both TCR and GGR to remove the damage ... the expression of DNA repair proteins and tumor platinum levels We show that NaAsO2 and hyperthermia either as single agents or in combination reverse key DNA repair protein responses to cisplatin...

Ngày tải lên: 20/06/2014, 07:20

... lymphopenia and microcephaly Disrupted DNA repair pathway DSB repair, DNA damage signalling DSB repair, DNA damage signalling DSB repair, DNA damage signalling DSB repair DSB repair, DNA damage signalling ... lead to DNA lesions which trigger DNA damage checkpoint response and DNA repair signalling The accumulation of unrepaired/unrepairable DNA damage within cells leads to a sustained DNA damage checkpoint ... machinery in response to DNA strand breaks J Cell Biol 173:195-206 Bekker-Jensen, S., and N Mailand 2010 Assembly and function of DNA double-strand break repair foci in mammalian cells DNA Repair (Amst)...

Ngày tải lên: 27/06/2014, 19:20

... lymphopenia and microcephaly Disrupted DNA repair pathway DSB repair, DNA damage signalling DSB repair, DNA damage signalling DSB repair, DNA damage signalling DSB repair DSB repair, DNA damage signalling ... lead to DNA lesions which trigger DNA damage checkpoint response and DNA repair signalling The accumulation of unrepaired/unrepairable DNA damage within cells leads to a sustained DNA damage checkpoint ... machinery in response to DNA strand breaks J Cell Biol 173:195-206 Bekker-Jensen, S., and N Mailand 2010 Assembly and function of DNA double-strand break repair foci in mammalian cells DNA Repair (Amst)...

Ngày tải lên: 28/06/2014, 03:20

... means that DNA breakage and repair occurs in a non-physiological environment Whitaker and McMillan reported in 1992 that encapsulating cells before irradiation impair the estimation of DNA repair ... estimate DNA repair Nevertheless, the underlying mechanisms affecting cells embedded in agarose during DNA repair in PFGE methodology are still not well understood To better understand spontaneous DNA ... Mahrhofer H, Burger S, Oppitz U, Flentje M, Djuzenova CS: Radiation induced DNA damage and damage repair in human tumor and fibroblast cell lines assessed by histone H2AX phosphorylation Int J Radiat...

Ngày tải lên: 09/08/2014, 09:20

... the repair kinetics of single and double strand DNA breaks induced in wild type and silenced lines Of the different forms of DNA damage, DSBs are one of the most cytotoxic and, if left unrepaired, ... understanding of the DNA double-strand break repair machinery of plants DNA Repair 2006, 5(1):1 Friesner J, Britt AB: Ku80- and DNA ligase IV-deficient plants are sensitive to ionizing radiation and ... Col0 and R2 =0.9365 atlig1-RNAi respectively) (B) Time course of SSB repair in Col0 and atlig1-RNAi lines over hour repair period Background DNA damage in untreated (control) seedlings and damage...

Ngày tải lên: 12/08/2014, 03:20

... genetic susceptibility in SLE [20] Bassi and colleagues, however, reported that SLE leucocytes less efficiently repair radiation-induced DNA damage and that DNA repair gene polymorphic sites may predispose ... Donadi E: Efficiency of the DNA repair and polymorphisms of the XRCC1, XRCC3 and XRCC4 DNA repair genes in systemic lupus erythematosus Lupus 2008, 17:988-995 Fernandez D, Perl A: Metabolic control ... to mitochondrial dysfunction Also worth noting is that repairs of UV-induced DNA damage by ERCC2/XPD and ERCC5/XPG requires ATP The ATP and DNA binding regions are contained in ERCC2/XPD [24] Most...

Ngày tải lên: 12/08/2014, 15:23

... glycosylase hOGGl and the E coli DNA glycosylase AlkA kink error-free DNA in the same way they kink DNA during the excision of a damaged base [15] It is thus likely that some DNA glycosylases ... happened to be bound to DNA downstream of the error at the time of damage or by one that subsequently binds to DNA downstream of the error and then scans the DNA until it finds the damaged base pair ... purposes) Theoretical Biology and Medical Modelling 2005, 2:15 repair enzymes, MutY and endonuclease III, are estimated to be 30 and 500 respectively and the number of MutM repair enzymes is estimated...

Ngày tải lên: 13/08/2014, 22:22

... checkpoint responses; however, the processing of many of these DNA structures generates single-stranded DNA, the salient intermediate in the DNA- damage checkpoint response In fact, double-strand DNA ... and because essential genes can be used in the assay Pan et al [4] take a wide view of the DNA- damage response, and include DNA replication, cell-cycle checkpoints and other contributors to DNA ... Singlestrand nicks Nuclease Singlestranded DNA Checkpoint response Cell-cycle arrest Increased dNTP pools Repair/ recombination Adaptation Figure Various pathways by which damage to DNA can elicit...

Ngày tải lên: 14/08/2014, 17:22

... of DNA damage, DNA repair mechanisms have evolved to eliminate or at the very least, minimize DNA damage Specific pathways have evolved to deal with distinct forms of DNA damage Base excision repair ... kinase-like protein kinases FAT FRAP, ATM, and TRRAP C-terminus Carboxy terminus dsDNA double-strand DNA ssDNA single-strand DNA SAP SAF-A/B, Acinus, and PIAS domain VHS Vps27p/Hrs/STAM XLF XRCC4-like ... Introduction 1.1 DNA Damage and Repair DNA is under constant assault which threatens the integrity of the genome Damaged DNA interferes with several cellular processes including transcription, DNA replication,...

Ngày tải lên: 24/08/2014, 09:33

... sequence-independent DNA binding activity, favoring double-stranded DNA over single-stranded DNA I demonstrated that the two proteins form a 1:1 stochiometric complex, and once formed, Metnase can localize to DNA ... cells, DNA double-strand breaks induced by IR and V(D)J recombination are mainly repaired by nonhomologous end-joining (NHEJ) (1-5) The NHEJ repair proteins Ku70/80, DNA PKcs, Artemis, and Xrcc4/ligase ... urea) for DNA cleavage DNA size makers indicated on the left B) Kinetic analysis of Metnase’s DNA cleavage activity with partial duplex DNA (3’- and 5’-overhangs) M1 and M2 represent two DNA markers...

Ngày tải lên: 24/08/2014, 10:50

... (NHEJ) DNA Repair Pathway A DSB occurs and the pathway is initiated by the Ku protein binding damaged DNA termini, translocates inward, and recruits DNA- PKcs to form the DNA- PK heterotrimer DNA- PK ... OF REDOX EFFECTS AND THE KU80 CTERMINAL REGION IN THE REGULATION OF THE HUMAN DNA REPAIR PROTEIN KU DNA double strand breaks (DSB) are among the most lethal forms of DNA damage and can occur as ... instability and contribute to the development and progression of cancer An increasing amount of research is drawing a close correlation between DNA repair and how it affects the development and treatment...

Ngày tải lên: 24/08/2014, 11:02