hdacs dna damage repair and radiation sensitivity
in situ detection of dna damage, methods and protocols
... Labeling DNA Damage with Terminal Transferase I LABELING DNA BREAKS USING TERMINAL TRANSFERASE (TUNEL ASSAY) Walker et al Labeling DNA Damage with Terminal Transferase Labeling DNA Damage with ... single stranded DNA molecules containing a 3'-OH and will attach nucleotides to a single-strand nick in DNA This is particularly useful since many single-strand breaks are also introduced into DNA ... study of DNA damage holds a wide interest within both basic and applied fields of research Elucidating the mechanisms involved in the generation of DNA damage, and the consequences of this damage, ...
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báo cáo hóa học:" Sodium arsenite and hyperthermia modulate cisplatin-DNA damage responses and enhance platinum accumulation in murine metastatic ovarian cancer xenograft after hyperthermic intraperitoneal chemotherapy (HIPEC)" pptx
... bulky DNA damage that is repaired mostly by the nucleotide excision repair system (NER) Cellular response to cisplatin -DNA damage involves the induction of DNA repair proteins to initiate DNA repair ... Following DNA damage recognition, downstream DNA repair proteins (XPA, RPA, TFIIH complex, ERCC1/XPF and XPG) are recruited to the DNA damage recognition complexes in both TCR and GGR to remove the damage ... hyperthermia alter the expression of DNA repair proteins and tumor platinum levels We show that NaAsO2 and hyperthermia either as single agents or in combination reverse key DNA repair protein responses...
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DNA REPAIR − ON THE PATHWAYS TO FIXING DNA DAMAGE AND ERRORS docx
... 87(15):5837–5841 18 DNA Repair − On the Pathways to Fixing DNA Damage and Errors Holmes, AM & Haber, J (1999) Double-strand break repair in yeast requires both leading and lagging strand DNA polymerases, ... Sgs1 helicase and two nucleases Dna2 and Exo1 resect DNA double-strand break ends, Cell 134(6):981-994 2 Synergy Between DNA Replication and Repair Mechanisms Maria Zannis-Hadjopoulos and Emmanouil ... essential role in several DNA repair pathways, such as base excision repair, nucleotide excision repair, and double-strand (ds) break and mismatch repair Recent work from prokaryotes and eukaryotes has...
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Báo cáo khoa học: "Radiation induced apoptosis and initial DNA damage are inversely related in locally advanced breast cancer patients" ppt
... between initial DNA damage, measured as DSB, and RIA Ionizing radiation (IR) kills cells by damaging the structure and function of genomic DNA The response of cells to this damage and their ability ... [29,30], and 2) cell-cycle checkpoints that provide time for repair and apoptosis [31] Depending on the severity of the DNA damage, cells may undergo apoptosis instead of attempting to repair the damage ... ability to restore DNA sequence integrity remains unclear Intrinsic radiosensitivity is correlated in a first approach to the ability of the cell to detect and repair DNA damages DSB can be induced...
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Báo cáo khoa học: "The use of caspase inhibitors in pulsed-field gel electrophoresis may improve the estimation of radiation-induced DNA repair and apoptosis" pdf
... before irradiation [8] However, this tactic means that DNA breakage and repair occurs in a non-physiological environment Whitaker and McMillan reported in 1992 that encapsulating cells before irradiation ... estimate DNA repair Nevertheless, the underlying mechanisms affecting cells embedded in agarose during DNA repair in PFGE methodology are still not well understood To better understand spontaneous DNA ... killing) and would rapidly recover their proliferative potential On the other hand, a significant smear would indicate a less aggressive behaviour due to low radiation repair and high sensitivity...
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Báo cáo khoa học: "Combined low initial DNA damage and high radiation-induced apoptosis confers clinical resistance to long-term toxicity in breast cancer patients treated with high-dose radiotherapy" pdf
... initial DNA damage and RIA in LABC patients [23] Taking into account the above background and our previously observations, we explored the clinical association between initial DNA damage and RIA ... variables, both considered as predictive factors for radiation toxicity, and normal tissue damage Conclusions Initial DNA double-strand breaks and radiationinduced apoptosis in peripheral blood lymphocytes ... predictor assays taking into account the initial DNA damage and radiation- induced apoptosis levels, and introduces new data which may help to understand and define the complex mechanisms behind the...
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Báo cáo y học: "A general framework for quantifying the effects of DNA repair inhibitors on radiation sensitivity as a function of dose" pptx
... modifiers of DNA repair upon four distinct components of the radiation response was achieved: (1) radiation- independent survival (parameter z, Equation 1), (2) low-dose radiation sensitivity (parameters ... effect of the drug on plating efficiency; α and β are the classical linear and quadratic radiosensitivity parameters; G and dC are the low-dose hyper -sensitivity parameters [14]; i is an indicator ... (parameters G and dc), (3) the linear component of cell survival (α), and (4) the quadratic component of cell survival (β) A data set comprising complex survival curves and varied responses to DNA repair...
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Checkpoint and Coordinated Cellular Responses to DNA Damage
... of DNA damage is coupled to a specific DNA repair pathway as well as the cell cycle regulatory apparatus Certain types of Checkpoint and Coordinated Cellular Responses to DNA Damage 73 DNA damage ... ATR-ATRIP, Rad17, and 9-1-1 complexes can independently recognize damage- induced DNA structures such as ssDNA and junctions of dsDNA/ssDNA Moreover, RPA appears to play important roles in the damage recognition ... interaction between ATR-ATRIP and RPA-ssDNA, and might contribute to the activation of ATR Transduction of DNA Damage Signals After ATM and ATR are activated by DNA damage, they phosphorylate numerous...
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Tài liệu Báo cáo khoa học: Pyruvate reduces DNA damage during hypoxia and after reoxygenation in hepatocellular carcinoma cells pptx
... causes DNA damage in hepatocytes Transplant Proc 26, 907–909 Moller P, Loft S, Lundby C & Olsen NV (2001) Acute hypoxia and hypoxic exercise induce DNA strand breaks and oxidative DNA damage ... number of DNA strand breaks (e.g radiotherapy and alkylating agents) Pyruvate might thus reduce the efficiency 5194 E Roudier et al of treatment by limiting DNA damage and favoring repair Pyruvate ... enhance DNA repair Pyruvate thus plays a role in DNA protection and repair in tumor HepG2 cells during hypoxia and after reoxygenation The mechanism might involve both antioxidant properties and...
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Báo cáo khoa học: DNA mismatch repair system Classical and fresh roles potx
... ExoVII [22] DNA polymerase III, single-stranded DNA- binding protein and DNA ligase carry out repair synthesis [3] Eukaryotic mismatch repair All eukaryotic organisms, including yeast, mouse and human, ... synthesized strand between the nick and the mismatch is carried out by four redundant single-strand DNAspecific exonucleases: the 3¢ fi 5¢ exonucleases ExoI and ExoX and the 5¢ fi 3¢ exonucleases RecJ and ... of mismatch repair proteins S.-H Jun et al Fig A simplified model of DNA damage response pathways that are dependent on mismatch repair (MMR) proteins MMR proteins bind to damaged DNA and recruit...
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Báo cáo khoa học: Apoptosis and autophagy: Regulation of apoptosis by DNA damage signalling – roles of p53, p73 and HIPK2 ppt
... p73 and HIPK2 in apoptosis N Bitomsky & T G Hofmann Fig Regulation of DNA damage- induced cell death by p53 and HIPK2 Genotoxic stress-induced DNA damage facilitates activation of the DNA damage- activated ... turn determines whether DNA lesions can, or cannot, be repaired As unrepaired DNA damage is characterized by constant activity of the DNA damage checkpoint kinase ATM and ⁄ or ATR, continuous ... cellular context and the extent of DNA damage – which determines whether or not damage is reparable – the activated DDR can trigger different cellular responses Mild DNA damage is usually handled through...
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Báo cáo khoa học: Chromatin dynamics at DNA replication, transcription and repair pdf
... helicase and is required for the repair of damaged DNA Thus, in agreement with a role of Tip60 in DNA repair, the expression of a mutant version of Tip60 causes defects in DNA repair and reduces ... the HM loci and the telomeres, a mild sensitivity to UV irradiation [65,66], and defects in kinetochore function, thus reflecting roles in heterochromatin formation, DNA damage repair and centromere ... larger portion of the DNA In NER, a short oligonucleotide including the damaged site is excised by nicking one DNA strand on either side of the damage and removing the intervening DNA including the...
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DNA REPAIR AND HUMAN HEALTH ppt
... lymphopenia and microcephaly Disrupted DNA repair pathway DSB repair, DNA damage signalling DSB repair, DNA damage signalling DSB repair, DNA damage signalling DSB repair DSB repair, DNA damage signalling ... lead to DNA lesions which trigger DNA damage checkpoint response and DNA repair signalling The accumulation of unrepaired/unrepairable DNA damage within cells leads to a sustained DNA damage checkpoint ... of DNA repair pathways 3.1 Base excision repair and single strand break repair Base excision repair (BER) is the major pathway responsible for handling the mutagenic and cytotoxic effects of DNA...
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DNA REPAIR AND HUMAN HEALTH doc
... lymphopenia and microcephaly Disrupted DNA repair pathway DSB repair, DNA damage signalling DSB repair, DNA damage signalling DSB repair, DNA damage signalling DSB repair DSB repair, DNA damage signalling ... lead to DNA lesions which trigger DNA damage checkpoint response and DNA repair signalling The accumulation of unrepaired/unrepairable DNA damage within cells leads to a sustained DNA damage checkpoint ... of DNA repair pathways 3.1 Base excision repair and single strand break repair Base excision repair (BER) is the major pathway responsible for handling the mutagenic and cytotoxic effects of DNA...
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Báo cáo khoa học: "Protective effect of the isoflavone equol against DNA damage induced by ultraviolet radiation to hairless mouse skin" potx
... dna 27 ,84 ,42 ,1 ta dna erusopxe VUSS fo DEM × dna DEM × retfa nim 03 dellik erew puorg tnemtaert hcae morf ecim owT :selpmas niks fo noitcelloC )sdnalrehteN( hcraeseR dooF dna noitirtuN ONT morf ... tnacifingis llits saw dna ,stniop emit emas eht ta )ylno VUSS( puorg lortnoc eht ot derapmoc ,)ylevitcepser sDPC fo noitcuder %62 dna ,%05 ,%45 gnilaever ;10.0 < p( sdrawretfa h 42 dna ta dna erusopxe ... mret-trohs etaredom retfa dna erofeb niks eht ot deilppa nehw ,louqe lacipot fo stceffe noitcetorp egamad AND elbissop eht yduts ot tseretni fo saw ti ,sisenegonicracotohp dna metsys enummi eht fo...
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Báo cáo khoa học: " Enhancement of radiosensitivity in human glioblastoma cells by the DNA N-mustard alkylating agent BO-1051 through augmented and sustained DNA damage response" potx
... phosphorylation, and kinetics of repair of DNA strand breaks in irradiated cervical cancer cell lines Cancer Res 2004, 64(19):7144-7149 47 Bilsland E, Downs JA: Tails of histones in DNA double-strand break repair ... important determinant of irradiation sensitivity [22,34] The cell cycle is strongly affected by DNA damage, and a cell’s radiosensitivity depends on cell cycle position and progression [22] Conventionally, ... enzymes, blocks CDK1 activation and causes a G2 arrest [39] Furthermore, ATM’s essential role in DNA damage and repair is highlighted by the extreme sensitivity to ionizing radiation of cells with defective...
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Báo cáo khoa học: " Enhanced radiation sensitivity and radiation recall dermatitis (RRD) after hypericin therapy – case report and review of literature" doc
... sensitise the skin to UV radiation or visible light (photosensitivity), x-rays (radiation sensitivity) or can induce a radiation recall dermatitis (RRD) (Tab 1) [6] Photosensitivity occurs both ... http://www.ro-journal.com/content/1/1/32 Table 1: Drugs associated with radiation sensitivity, RRD and photosensitivity [6] Increased radiation sensitivity and RRD Adriamycin Bleomycin Dactinomycin Daunorubicin Docetaxel Doxorubicin ... USA and 6.7 billion dollars in Page of (page number not for citation purposes) Radiation Oncology 2006, 1:32 http://www.ro-journal.com/content/1/1/32 Table 1: Drugs associated with radiation sensitivity, ...
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Báo cáo y học: "Detection of Chlamydia trachomatis-DNA in synovial fluid: evaluation of the sensitivity of different DNA extraction methods and amplification systems" pps
... by standard regression analysis The number of C trachomatis EBs and C trachomatis PBMOs measured by immunofluorescence were the basis for determining sensitivity For the PCR and LCR assays, sensitivity ... evaluated for their feasibility and sensitivity to detect C trachomatis DNA in SF in clinical practice We therefore analyzed five previously published DNA extraction methods and five amplification systems ... of clinical samples, culture of C trachomatis and performed DNA extraction, PCR analysis and drafted the manuscript IB and SM performed parts of DNA extraction as well as parts of PCR analysis...
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Báo cáo y học: " Physical and in silico approaches identify DNA-PK in a Tax DNA-damage response interactome" pot
... which DNA- PKcs is a member (DNA- PKcs, PCNA, PARP, Ku70, Ku86, and TRBP) Thus, DNA- PKcs demonstrates an increased rank when consideration is refocused toward protein interactions involved in DNA damage ... second neighborhood network restricted to DNA damage repair response The 3-core representation of the G2* second neighborhood network restricted to DNA damage repair response Shown is the result of ... our analysis to the DNA damage repair response Thus, we asked which of these known protein interactions has a known function that would potentially impact the cellular DNA repair response process...
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Báo cáo y học: "Ubiquitination of HTLV-I Tax in response to DNA damage regulates nuclear complex formation and nuclear export" pps
... (upper Tax band) to total Tax (upper and lower Tax bands) for both unirradiated and UV irradiated cells These results demonstrate that mono-ubiquitination of Tax occurs following DNA damage and that ... residues 280 and 284 are ubiquitinated in response to DNA damage Since Tax is ubiquitinated in response to DNA damage (Figure 2) and its subsequent translocation to the cytoplasm following UV irradiation ... after irradiation, Tax was immunoprecipitated, and levels of Tax and UB-Tax and were examined by immunoblot using α-Tax (Figure 4) and α-HA (data not shown) As expected, following UV irradiation...
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