dna damage and repair assays

... 87(15):5837–5841 18 DNA Repair − On the Pathways to Fixing DNA Damage and Errors Holmes, AM & Haber, J (1999) Double-strand break repair in yeast requires both leading and lagging strand DNA polymerases, ... Sgs1 helicase and two nucleases Dna2 and Exo1 resect DNA double-strand break ends, Cell 134(6):981-994 2 Synergy Between DNA Replication and Repair Mechanisms Maria Zannis-Hadjopoulos and Emmanouil ... essential role in several DNA repair pathways, such as base excision repair, nucleotide excision repair, and double-strand (ds) break and mismatch repair Recent work from prokaryotes and eukaryotes has...

Ngày tải lên: 28/06/2014, 05:20

... myelin repair seen in the NG2 null mouse Similarly, it is possible that pericytes affect both myelin damage and repair The recruitment of pericytes for revascularization of the lesion and repair ... OPCs, pericytes, and macrophages/microglia The differential contributions of these three cell types to the damage and repair processes, Table Proliferation of PDGFRa, PDGFRb, and IBA-1 expressing ... contribute to myelin repair by clearing myelin debris and by producing cytokines and growth factors that promote recruitment of OPCs and prime interactions between OPCs and axons Thus, NG2-dependent...

Ngày tải lên: 19/06/2014, 22:20

... initial DNA damage and RIA in LABC patients [23] Taking into account the above background and our previously observations, we explored the clinical association between initial DNA damage and RIA ... to develop new predictor assays taking into account the initial DNA damage and radiation-induced apoptosis levels, and introduces new data which may help to understand and define the complex mechanisms ... experiments and data acquisition MIN has been involved in conception and design of the study and has made the DNA- DSB experiments and analyses PCL has been involved in conception and design of...

Ngày tải lên: 09/08/2014, 09:20

... GROWING DNA POLYMER ENTERING dTTP DNA TEMPLATE dNTP GẮN VÀO CHUỖI DNA ĐANG KÉO DÀI TẠI VỊ TRÍ 3’ Các thành phần tham gia trình nhân đôi DNA dnaA Primasome dnaB dnaC dnaG DNA gyrase Rep protein SSB DNA ... the content of T, and the content of G equals the content of C in double-stranded DNA from any species Mạch đôi DNA 5’ 3’ Rãnh Rãnh phụ “B” DNA 3’ 5’ 3’ 5’ DNA LÀ CỐT LÕI CỦA NST DNA LÀ CỐT LÕI ... III DNA pol I DNA ligase gắn vào trình tự DNA mạch gốc helicase (tháo xoắn DNA sợi gốc) gắn dnaB primase (tổng hợp RNA primer) nhận biết đầu âm cuộn xoắn DNA ngã nhân đôi helicase (tháo xoắn DNA...

Ngày tải lên: 09/11/2016, 04:25

... of DNA damage is coupled to a specific DNA repair pathway as well as the cell cycle regulatory apparatus Certain types of Checkpoint and Coordinated Cellular Responses to DNA Damage 73 DNA damage ... ATR-ATRIP, Rad17, and 9-1-1 complexes can independently recognize damage- induced DNA structures such as ssDNA and junctions of dsDNA/ssDNA Moreover, RPA appears to play important roles in the damage recognition ... interaction between ATR-ATRIP and RPA-ssDNA, and might contribute to the activation of ATR Transduction of DNA Damage Signals After ATM and ATR are activated by DNA damage, they phosphorylate numerous...

Ngày tải lên: 25/10/2013, 21:20

... causes DNA damage in hepatocytes Transplant Proc 26, 907–909 Moller P, Loft S, Lundby C & Olsen NV (2001) Acute hypoxia and hypoxic exercise induce DNA strand breaks and oxidative DNA damage ... number of DNA strand breaks (e.g radiotherapy and alkylating agents) Pyruvate might thus reduce the efficiency 5194 E Roudier et al of treatment by limiting DNA damage and favoring repair Pyruvate ... enhance DNA repair Pyruvate thus plays a role in DNA protection and repair in tumor HepG2 cells during hypoxia and after reoxygenation The mechanism might involve both antioxidant properties and...

Ngày tải lên: 18/02/2014, 16:20

... p73 and HIPK2 in apoptosis N Bitomsky & T G Hofmann Fig Regulation of DNA damage- induced cell death by p53 and HIPK2 Genotoxic stress-induced DNA damage facilitates activation of the DNA damage- activated ... turn determines whether DNA lesions can, or cannot, be repaired As unrepaired DNA damage is characterized by constant activity of the DNA damage checkpoint kinase ATM and ⁄ or ATR, continuous ... cellular context and the extent of DNA damage – which determines whether or not damage is reparable – the activated DDR can trigger different cellular responses Mild DNA damage is usually handled through...

Ngày tải lên: 16/03/2014, 00:20

... helicase and is required for the repair of damaged DNA Thus, in agreement with a role of Tip60 in DNA repair, the expression of a mutant version of Tip60 causes defects in DNA repair and reduces ... larger portion of the DNA In NER, a short oligonucleotide including the damaged site is excised by nicking one DNA strand on either side of the damage and removing the intervening DNA including the ... indirectly be required to repair defective DNA Restoring chromatin after repair Restoration of chromatin structure is an important final step in the repair of damaged DNA and must ensure that all...

Ngày tải lên: 16/03/2014, 16:20

... Labeling DNA Damage with Terminal Transferase I LABELING DNA BREAKS USING TERMINAL TRANSFERASE (TUNEL ASSAY) Walker et al Labeling DNA Damage with Terminal Transferase Labeling DNA Damage with ... single stranded DNA molecules containing a 3'-OH and will attach nucleotides to a single-strand nick in DNA This is particularly useful since many single-strand breaks are also introduced into DNA ... study of DNA damage holds a wide interest within both basic and applied fields of research Elucidating the mechanisms involved in the generation of DNA damage, and the consequences of this damage, ...

Ngày tải lên: 11/04/2014, 09:47

... bulky DNA damage that is repaired mostly by the nucleotide excision repair system (NER) Cellular response to cisplatin -DNA damage involves the induction of DNA repair proteins to initiate DNA repair ... Following DNA damage recognition, downstream DNA repair proteins (XPA, RPA, TFIIH complex, ERCC1/XPF and XPG) are recruited to the DNA damage recognition complexes in both TCR and GGR to remove the damage ... hyperthermia alter the expression of DNA repair proteins and tumor platinum levels We show that NaAsO2 and hyperthermia either as single agents or in combination reverse key DNA repair protein responses...

Ngày tải lên: 20/06/2014, 07:20

... between initial DNA damage, measured as DSB, and RIA Ionizing radiation (IR) kills cells by damaging the structure and function of genomic DNA The response of cells to this damage and their ability ... [29,30], and 2) cell-cycle checkpoints that provide time for repair and apoptosis [31] Depending on the severity of the DNA damage, cells may undergo apoptosis instead of attempting to repair the damage ... individual, and the amount of initial DNA damage depends on each patient The two main mechanisms of DSB repair are 1) non-homologous end joining (NHEJ) and homologous recombination repair (HRR)...

Ngày tải lên: 09/08/2014, 09:20

... phosphorylation, and kinetics of repair of DNA strand breaks in irradiated cervical cancer cell lines Cancer Res 2004, 64(19):7144-7149 47 Bilsland E, Downs JA: Tails of histones in DNA double-strand break repair ... double-strand DNA damage and complex with other important repair molecules g-H2AX is required for foci formation for numerous factors including p53, MRN complex (MRE-11, RAD50, and NBS1), and BRCA1 ... produce DNA interstrand and/ or intrastrand cross-links [29,30] As has been known, bifunctional alkylating agents induce collapsed replication forks that can lead to either cell cycle arrest, DNA repair, ...

Ngày tải lên: 09/08/2014, 09:20

... the repair kinetics of single and double strand DNA breaks induced in wild type and silenced lines Of the different forms of DNA damage, DSBs are one of the most cytotoxic and, if left unrepaired, ... understanding of the DNA double-strand break repair machinery of plants DNA Repair 2006, 5(1):1 Friesner J, Britt AB: Ku80- and DNA ligase IV-deficient plants are sensitive to ionizing radiation and ... Col0 and R2 =0.9365 atlig1-RNAi respectively) (B) Time course of SSB repair in Col0 and atlig1-RNAi lines over hour repair period Background DNA damage in untreated (control) seedlings and damage...

Ngày tải lên: 12/08/2014, 03:20

... which DNA- PKcs is a member (DNA- PKcs, PCNA, PARP, Ku70, Ku86, and TRBP) Thus, DNA- PKcs demonstrates an increased rank when consideration is refocused toward protein interactions involved in DNA damage ... second neighborhood network restricted to DNA damage repair response The 3-core representation of the G2* second neighborhood network restricted to DNA damage repair response Shown is the result of ... our analysis to the DNA damage repair response Thus, we asked which of these known protein interactions has a known function that would potentially impact the cellular DNA repair response process...

Ngày tải lên: 13/08/2014, 05:21

... (upper Tax band) to total Tax (upper and lower Tax bands) for both unirradiated and UV irradiated cells These results demonstrate that mono-ubiquitination of Tax occurs following DNA damage and that ... to DNA damage may be a consequence of their inability to be ubiquitinated Lysine residues 280 and 284 are ubiquitinated in response to DNA damage Since Tax is ubiquitinated in response to DNA damage ... (Figure 5E and 6B) where it was colocalized with sc35 (Figure 5E and 6C) As with wild-type Tax, following DNA damage, increased cytoplasmic localization of TaxK6R (Figure 5F and 6B) and reduced...

Ngày tải lên: 13/08/2014, 06:20

... glycosylase hOGGl and the E coli DNA glycosylase AlkA kink error-free DNA in the same way they kink DNA during the excision of a damaged base [15] It is thus likely that some DNA glycosylases ... happened to be bound to DNA downstream of the error at the time of damage or by one that subsequently binds to DNA downstream of the error and then scans the DNA until it finds the damaged base pair ... purposes) Theoretical Biology and Medical Modelling 2005, 2:15 repair enzymes, MutY and endonuclease III, are estimated to be 30 and 500 respectively and the number of MutM repair enzymes is estimated...

Ngày tải lên: 13/08/2014, 22:22

... (uninfected and the LacZ control cells and cells knocked-down for Rel-A, p53 and ATM), each probed at two time points: without treatment and h after exposure to NCS Definition of the damage- responding ... by RNAi Nat Biotechnol 2003, 21:635-637 Povirk LF: DNA damage and mutagenesis by radiomimetic DNA- cleaving agents: bleomycin, neocarzinostatin and other enediynes Mutat Res 1996, 355:71-89 Irizarry ... A, Shamir R: CLICK and EXPANDER: a system for clustering and visualizing gene expression data Bioinformatics 2003, 19:1787-1799 EXPANDER [http://www.cs.tau.ac.il/~rshamir/expander/] Dennis G Jr,...

Ngày tải lên: 14/08/2014, 14:21

... of influenza infection and inflammation on DNA damage and DNA damage responses By studying chromatin phosphorylation as a measure of DNA damage, we show that the level of DNA damage increases following ... possibility that DNA damage and DNA repair modulate the severity of influenza-induced cytotoxicity, thereby affecting tissue damage and regeneration, and ultimately disease outcome Materials and methods ... of DNA damage generated by endogenous stresses are single strand lesions, such as base damage, abasic sites and SSBs [39, 40] While DNA strand breaks can arise directly via the cleavage of DNA...

Ngày tải lên: 09/09/2015, 08:16

... sensitivity, and (III) a single-strand DNA (ssDNA) standard bearing the same sequence and length as of the target gene was created and accurately quantified for use in the PCR-free quantitative assays ... sensitive and quantitative tool alternate to the RQPCR for early disease diagnosis (III) Preparation of ssDNA standard for quantitative assays A ssDNA standard bearing the same sequence and length ... detection of the p185-ssDNA by both CNT-based label and bDNA hybridization assays indicated good integrity and functionality of the standard The approach offers a means to prepare standard suitable for...

Ngày tải lên: 12/09/2015, 09:28

... checkpoints, and the G2/M DNA damage checkpoint The G1 and G2 checkpoints respond to some types of localized physical damage to the DNA and are known as DNA damage checkpoints In S.cerevisiae, the G1 DNA ... Phase and G2/M Checkpoints: In the budding yeast, DNA damage surveillance mechanisms operate in G1, S and G2 phases These are the G1 DNA damage checkpoint, the S phase replication and DNA damage ... replication checkpoint and DNA damage checkpoint , we enquired whether DNA damage checkpoint pathway also target spindle to restrain the segregation of damaged chromosomes until they are repaired Our results...

Ngày tải lên: 14/09/2015, 14:03