apoptosis and dna damage in human spermatozoa

... the cell nucleus and the key molecules p53, p73 and homeodomain-interacting protein kinase (HIPK2) Multiple functions of p53 in DNA damage- induced apoptosis The tumour suppressor and transcription ... an increased SUMOylation and stability of PML, suggesting an additional role of HIPK2 in regulating DNA damageinduced cell death Collectively, these findings indicate that HIPK2 is involved in DNA ... p73 and HIPK2 in apoptosis N Bitomsky & T G Hofmann Fig Regulation of DNA damage- induced cell death by p53 and HIPK2 Genotoxic stress-induced DNA damage facilitates activation of the DNA damage- activated...

Ngày tải lên: 16/03/2014, 00:20

... formation of huntingtin-containing inclusion bodies in Huntington’s disease encourages cell survival, whereas monomers and small inclusion bodies of huntingtin, a protein possessing abnormal polyQ ... aB-Crystallin R120 corresponds to aA-crystallin R116 and both are conserved a-crystallin domain arginines R120G aB-crystallin permits intermediate filament self association in vitro, although binding ... aAcrystallin requires this domain [66] Attachment of R116C aA-crystallin to Hsp27 and aB-crystallin increases in comparison to wild type, while binding to cC-crystallin and bB2-crystallin decreases...

Ngày tải lên: 19/02/2014, 18:20

... worked in reducing maternal mortality include those relating to innovative financing measures; investment in human resources, both in terms of strengthening pre-service education and emphasizing in- service ... NGOs and other institutions Improving maternal and perinatal health working in the field on maternal and infant health Work with Doctors with Africa CUAMM, an NGO operating in Africa since 1955 in ... Hypertension in Pregnancy (ISSHP), in Geneva WHO will be leading and coordinating a number of sessions in both Congresses increasing visibility and repositioning HRP's work and priorities in the global...

Ngày tải lên: 23/03/2014, 06:20

... turn recruits other proteins, including paxillin, p130Cas, vinculin and talin in focal adhesions These protein complexes anchor the actin cytoskeleton and provide structural integrity to cells [16] ... either tyrosine kinase inhibitor, genistein, or MAP kinase inhibitor, PD98059 Inhibition of tyrosine kinase by genistein greatly inhibited LPA-induced PAK activation, whereas MAP kinase activity ... endothelial cells [52] and G protein coupled receptor kinase (GRK)-interacting targets (GIT) links PAK and FAK [53], involvement of PIX, Nck and GIT in LPA-induced FAK phosphorylation and cell motility...

Ngày tải lên: 30/03/2014, 13:20

... MAPK In contrast, inhibition of p38 by a specific inhibitor SB203580 decreased apoptosis while ERK1/2 inhibition by a specific inhibitor PD98059 resulted in an increase of apoptosis in H pylori-infected ... expression using PAR-2 AS ODN in AGS cells Additionally, inhibition of PAR-2 activity using trypsin inhibitor increased cell death and DNA fragmentation in H pylori-infected AGS cells These results ... activation of multiple signaling pathways, including MAPK cascades in distinct cell types [16,17] PAR-2 is involved in cell proliferation and apoptosis in several cell types [18,19] Recent data...

Ngày tải lên: 18/06/2014, 16:20

... Materials and methods Cells and chemicals Human hepatoma Huh7 cells were grown in DMEM containing 10% FBS, 1× penicillin, streptomycin, fungizone, 10mM L-glutamine, and 1× non-essential amino acids ... signaling and increased HCV replication in the presence of IFN Inhibition of Jak-Stat signaling may involve ethanol perturbation of IFN-α induced changes in membrane fluidity, inhibition of IFN binding ... Ras-ERK, and phosphoinositol kinase (PI3K) [36-40] However, p38 kinase activity, which is important in IFN-α and IFN-γ induced transcription, is not involved in IFN induced Stat1 serine phosphorylation...

Ngày tải lên: 19/06/2014, 08:20

... Materials and methods Cells and chemicals Human hepatoma Huh7 cells were grown in DMEM containing 10% FBS, 1× penicillin, streptomycin, fungizone, 10mM L-glutamine, and 1× non-essential amino acids ... signaling and increased HCV replication in the presence of IFN Inhibition of Jak-Stat signaling may involve ethanol perturbation of IFN-α induced changes in membrane fluidity, inhibition of IFN binding ... Ras-ERK, and phosphoinositol kinase (PI3K) [36-40] However, p38 kinase activity, which is important in IFN-α and IFN-γ induced transcription, is not involved in IFN induced Stat1 serine phosphorylation...

Ngày tải lên: 20/06/2014, 04:20

... species in regeneration, shows damage of 32% of individuals in the investigated area (open space 34% of damaged individuals, below the stand 32% of damaged individuals) whilst average damage in the ... at summarizing the damage (browsing, peeling) and at expressing both types of damage together As average values from the preceding maps of browsing and peeling were used as a basis, damage is ... assessment of peeling and browsing intensity was carried out in two categories including individuals with dbh from cm to 11.9 cm and individuals with dbh from 12 cm to 20 cm Individuals with dbh...

Ngày tải lên: 07/08/2014, 03:22

... cutting tool The ideal instrument would accomplish the necessary functions of cutting and coagulation while minimizing collateral tissue injury Decreasing overall tissue injury has obvious clinical ... first introduced into Otolaryngology by Jako and Strong in 1972 [7] At a wavelength of 10,600 nm, this laser is rapidly absorbed by the water in the tissues ensuring minimal thermal damage and ... and incisions were made in the tongue akin to a tongue tumor resection using the harmonic scalpel and flexible C02 laser fiber at recommended settings of 5W for the harmonic scalpel and settings...

Ngày tải lên: 09/08/2014, 02:20

... to inducing apoptosis of the synovium, intra-articular injection of exogenous TRAIL protein reduced the leukocytic infiltrate into the inflamed joints, indicating that TRAIL may inhibit joint inflammation ... relatively inefficient in inducing synovial apoptosis, requiring a high multiplicity of infection to confer apoptosis in culture Thus it appears as if TRAIL, either administered as a recombinant protein ... transfer, is relatively ineffective in inducing apoptosis of both rabbit and human synovial fibroblasts in culture In contrast to the cell culture results using human and rabbit synovial fibroblasts,...

Ngày tải lên: 09/08/2014, 07:20

... following invasion and degradation of surrounding matrix, and migration into the vasculature and surrounding tissue, E-cadherin catenin complex is reintroduced and cells adhere to the vasculature and ... for positivity and negativity We determined the expression of E-cadherin, α-, β- and γcatenin in human breast carcinomas using real time quantitative polymerase chain reaction and investigated ... relationship of E-cadherin, α-catenin, β-catenin and γ-catenin in breast tumours to Nottingham Prognostic Index (NPI) (E-cadherin p = 0.094, αcatenin p = 0.144, β-catenin p = 0.378, γ-catenin p = 0.131)...

Ngày tải lên: 09/08/2014, 07:21

... paraformaldehyde in PBS (pH 7.4) and mounted in Vectashield mounting medium with 1.5 Ag/mL 4V, 6-diamidino-2phenylindole (Vector Laboratories, Inc., Burlingame, CA) The cells were stained by method ... again with PBS and resuspended in PBS containing PI and RNase A The samples were subjected to a FACScaliber flow cytometer and data analysis was done using CellQuest Pro software (Becton Dickinson, ... separated apart and, instead, aggregated in the center of the nucleus in a round cell contour These findings indicated a mitotic arrest at the metaphase was induced by costunolide Signaling molecules...

Ngày tải lên: 09/08/2014, 09:20

... confluency DNA- binding proteins were cross-linked to DNA by adding 1% formaldehyde for 10 at 37°C Cells were washed twice with ice-cold PBS containing protease inhibitors (1 mM PMSF, μg/mL aprotinin and ... G2/M DNA- damage checkpoint [28] The G2/M arrest induced by DCQ and the S-phase accumulation induced by IR appear together in combination treatments Despite our intriguing findings that the combination ... was cultured in growth media containing RPMI 1640 with L-glutamine and 25 mm HEPES, supplemented with 10% FBS and 1% penicillin-streptomycin (50 μg/mL), and incubated in a humidified incubator (95%...

Ngày tải lên: 09/08/2014, 10:20

... chemokine ligand; CXCL, C-X-C chemokine ligand; IFIG, IFN-inducible gene; IFIT, interferon-induced protein with tetratricopeptide repeats; IL, interleukin; IP-10, interferon-inducible protein 10; ... chemokine scores in SLE patients with organ damage Given that chemokines are involved in tissue damage and inflammation, we next explored whether chemokine scores are related to organ damage in ... Mampaso F, Schlondorff D: Chemokine expression precedes inflammatory cell infiltration and chemokine receptor and cytokine expression during the initiation of murine lupus nephritis J Am Soc Nephrol...

Ngày tải lên: 09/08/2014, 13:22

... remained almost intact The findings support the view that T614 can protect the joints from damage in an inflammatory environment, in concert with MTX Proinflammatory cytokines TNF-α, IL-1β, and ... disease was indicated by increased edema and erythema of one or both ankle joints, followed by involvement of the metatarsal and interphalangeal joints Fully developed arthritis, including red and swollen ... sharply in the PBMCs from highdose T-614 and combination treated rats In particular, T-614 inhibited the elevated IL-17 expression in inguinal lymph node cells in a dose-dependent manner IFN-γ and...

Ngày tải lên: 09/08/2014, 13:22

... Fagerhol MK, Landewe R, Heijde D van der, Uhlig T, Mowinckel P, Kvien TK: Calprotectin (a major leucocyte protein) is strongly and independently correlated with joint inflammation and damage in rheumatoid ... relationship to joint and vascular damage and risk factors in RA patients [19] We report associations of each protein with both joint and vascular disease and their risk factors Materials and methods ... erosions and joint space narrowing using the modified Sharp score [47] Genotyping High resolution human leukocyte antigen (HLA)-DRB1 genotyping was carried out on buffy coat DNA using PCR and sequence-specific...

Ngày tải lên: 09/08/2014, 13:22

... formation and TFIIB binding in vivo The high prevalence for the occurrence of motif combinations in TFIIB-dominated genes in illustrated in Figure 5e In line with the known synergy between INR and ... (black line) and replicate (grey line) relative to aligned TSSs at 10-bp resolution Binding of TBP and the initiating form of RNAPII (phosphorylated at serine in its carboxy-terminal domain) were ... one initial cycle consisting of minutes at 55° (during which polymerase was added), minutes at 72°C and minutes at 94°C, followed by 22 cycles of 0.5 minutes at 94°C, 0.5 minutes at 60°C and minutes...

Ngày tải lên: 09/08/2014, 20:21

... pyruvate (Invitrogen), MEM vitamins (Invitrogen), 50 U/ml penicillin/streptomycin (Invitrogen), and 50 nM 2-mercaptoethanol (Invitrogen) The human cervical carcinoma cell line HeLa was maintained in ... model in which the TATA box binding protein-associated factor TAF12 recruits Gadd45a (growth arrest and DNA- damage induced-a) and the nucleotide excision repair machinery to promoters, resulting in ... Recombinant human interleukin-3 and granulocytemacrophage colony-stimulating factor show common biological effects and binding characteristics on human monocytes Blood 1989, 74:2349-2359 25 Finnin M,...

Ngày tải lên: 09/08/2014, 20:22

... cDNA prepared and RT-PCR performed for 30 cycles using primers for TNF-α and β-actin obtained from R & D Systems Thus, several studies suggest that minimizing TNF levels in both HIV and M Tb-infected ... TNF inhibited p24 release induced by mycobacteria In a recent review Collins et al [4] postulate that higher viral loads, increased HIV diversity, and changes in cytokine/chemokine levels in HIV-infected ... goes untreated in HIV+ patients, most will die within one year M Tb is the most common opportunistic infection occurring in HIV-infected individuals in resource poor countries and it accelerates...

Ngày tải lên: 10/08/2014, 05:20

... significantly inhibits PGE-induced activation of JNK1/2 within 30 min, and suppressed PGE2-induced expression of uPA and MMP-9 within 24 h in human LoVo colon cancer cells (Figure 4B) 17b-Estradiol Inhibits ... of proteins by immunoblotting assay Phosphorylation of JNK1/2 was significantly induced within 15 in response to PGE2 stimulation, and was maintained up for 24 h (Figure 3B) The findings suggested ... functionally related zinc-containing enzymes that include interstitial collagenases, gelatinases, stromelysin, matrilysin, metalloelastase, and membrane-type MMPs [7,8] Upregulation of MMP-2 and MMP-9 has...

Ngày tải lên: 10/08/2014, 10:20