Functional analysis of metallothionein 2a isoform in breast cancer

... Tell-tale signs of breast cancer other than presence of lumps include thickening in an area of the breast, change in the size or shape of the breast, dimpling of the skin, change in shape of the nipple, ... impact on breast cancer has not been elucidated Furthermore, there are ten functional isoforms in human, with MT -2A being the most abundant isoform in...

Ngày tải lên: 14/09/2015, 14:08

... group of GHMP kinases in which proton transfer does not play a major role in the rate determining step of catalysis That five of the 10 disease-causing mutations resulted in insoluble protein in ... play a major role in the rate-determining step of catalysis Several of the disease-causing mutations are not soluble following induction in E coli Although all the mut...

Ngày tải lên: 08/03/2014, 02:20

... Timson Disease-causing mutations in human GALE Fig Crosslinking of wild-type and disease-causing mutants of UDP-galactose 4-epimerase (GALE) Crosslinking was carried out with N-(3dimethylaminopropyl)-N¢-ethylcarbodiimide ... during purification Kinetic analysis of disease-causing mutations The steady-state kinetic parameters of each of the nine disease-causing m...

Ngày tải lên: 23/03/2014, 11:20

... STRUCTURAL AND FUNCTIONAL ANALYSIS OF CRITICAL PROTEINS INVOLVED IN mRNA DECAY CHENG ZHIHONG (B.Sc) Ease China University of Science and Technology A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR ... deadenylation, decapping and degradation of the mRNA body Three proteins, hUpf1, Dhh1 and Ski8 involved in eukaryotic mRNA decay were structurally and f...

Ngày tải lên: 14/09/2015, 22:22

... curatively treated breast cancer patients [10] to address whether the EQ-5D is responsive to changes in HRQoL in a population of breast cancer patients in their first year after treatment Methodology ... Conclusion In this study the responsiveness of the EQ-5D was investigated for its use in economic evaluations of health interventions in bre...

Ngày tải lên: 18/06/2014, 19:20

... binding domains and ATPbinding domains, is illustrated in Figure Single marker associations Risk for developing breast cancer The association between any variant in the ATM gene and risk of breast ... mutations of either one of the three serine residues (367, 1893 and 1981) give rise to proteins defective in ATM signalling in vivo [24] Studies of l...

Ngày tải lên: 09/08/2014, 10:21

... Metallothionein- 2A silencing In Proceedings of the AACR 101st Annual Meeting 17-21 April 2010, Washington DC, USA Lai Y, Koo CY, Yip GW, Bay BH Over -expression of Metallothionein- 2A isoform increases ... transformation of breast tissues Table Functional significance of MT isoforms in breast cancer MT Isoforms MT-1A Expression Level in Cells Low Expression Level in...

Ngày tải lên: 11/09/2015, 10:03

... activity of DEC1 is also required for the interaction with BMAL1 Binding of DEC1 mutants to a CACGTG E-box To examine the binding ability of DEC1 mutants to the CACGTG E-box in the Dec1 promoter, ... for DEC1 suppressive activity against CLOCK/BMAL1-induced transcription In addition, the N-terminal region of DEC1, including the bHLH domain, interacted with...

Ngày tải lên: 19/02/2014, 16:20

... the C-terminal domain, the E coli Pta N-terminal domain is involved in stabilization of the hexameric native structure, in expression of the maximum catalytic activity, and in allosteric regulation ... Results Expression and purification of E coli Pta and truncated Ptas containing the C-terminal end By analysis of the protein domain architecture of E coli Pta...

Ngày tải lên: 06/03/2014, 11:20

... transfected individually or in combination into ldlD cells and immunoprecipitation was carried out with myc antibody using detergent-soluble cell lysates containing the amount of protein indicated ... 4¢-epimerase mutations associated with the intermediate form of type III galactosaemia J Inherit Metab Dis 31, 108–116 Timson DJ (2005) Functional analysis of disease-cau...

Ngày tải lên: 07/03/2014, 00:20

... report on the isolation of four new monoterpene synthase cDNAs by random sequencing of a flavedo-derived cDNA library of C limon and their characterization by functional expression in Escherichia ... sabinene, a-terpinene (E )-b-ocimene, terpinolene, linalool and a-terpineol cDNA isolation and sequencing Random sequencing of a cDNA library made from mRNA isolate...

Ngày tải lên: 08/03/2014, 23:20

... regulators in S coelicolor Fig Comparative alignment of domains (DNA–protein interaction), (dimerization and metal binding) and (containing a nonconserved amino acid stretch), of the Streptomyces coelicolor ... on DNAÆprotein interaction and of domain in the protein dimerization and metal binding (see the Discussion) There are important differences between DmdR1 and...

Ngày tải lên: 16/03/2014, 18:20

... Uncovering the mechanism of 5-FU resistance and characterization of pyrimidine biosynthesis enzymes in C elegans will help to further our understanding of pyrimidine biosynthesis enzymes by filling in ... metabolic enzymes UP mediates the first step of 5-FU conversion We searched for homologs of the human pyrimidine biosynthesis enzymes, including uridin...

Ngày tải lên: 30/03/2014, 01:20

... question of detecting therapy-related changes in the mass profiles registered for blood samples collected from breast cancer patients SELDI-ToF analysis of the plasma proteome of breast cancer patients ... Pietrowska et al., Mass spectrometry-based analysis of therapy-related changes in serum proteome patterns of patients with earlystage br...

Ngày tải lên: 18/06/2014, 16:20

... be one of the causes of high expression of the drug-metabolising enzyme CYP1B1 in cancerous tissues [14] Finally, as a tumor suppressor in breast cancer cells, miR-17-5p regulates breast cancer ... Ras-GAP binding proteins (G3BP) Oncogene 2007, 26(29):4209-4215 27 French J, Stirling R, Walsh M, Kennedy HD: The expression of Ras-GTPase activating protein SH3 domain-bind...

Ngày tải lên: 10/08/2014, 10:20