functional analysis of the cervicalfunctional analysis of intergenic

... Chapter one: The introduction – presents the rationale of the study, the aims of the study, scope of the study, methods of the study, data collection, and the research design  Chapter two: The literature ... Aims of the Study With in the framework of the paper, the study attempts to:  Re-examine some of the most important issues related to the experiential aspect of functional grammar  Analyze the ... called Theme and Rheme The Theme “served as the point of departure of the message” (Halliday 1994:37), which in English coincides with the initial element(s) of the clause; and the Rheme is the

Ngày tải lên: 07/09/2013, 13:48

... the (door) the (ladder) the (planet) the (woman) her (arm) It she the (man) we the (air) It them their they the (plants) they they their the (man) the (controls) he I the ... describing the state of being of the things under the observation of the astronauts in the new planet. Most of the processes are in the simple past tense (25/36). They are used in the narrative ... the writer and the reader(s), and the other between the characters themselves. The analysis shows that most of the Subjects in the clauses of the text are personal. Apart from the fictional

Ngày tải lên: 12/02/2014, 20:20

... that the basic region, particularly the conserved Arg65, but not His57, is essential for the suppressive activity of DEC1 Determination of the binding domain of DEC1 to BMAL1 The interaction of ... importance of the bHLH region of DEC1 The HXXXXXXXR sequence in the basic region has been reported to be conserved among group B bHLH proteins [26,27] We therefore examined whether these amino ... ng of each) Transfection of 0.1 ng of the DEC1 expression vector did not significantly decrease the transcriptional activity of CLOCK/BMAL1 (Fig 1B) Transfection of ng of the expression vector resulted

Ngày tải lên: 19/02/2014, 16:20

... these, there is the possibility that, through the effect of the proximities of the antibody-binding and Fn-binding sites, the interaction of the repeat with Fn may be sterically hindered in the ... by the injection of soluble repeats or 10 of both FnBPA and FnBPB across the sensor chip surface Table reports the kinetic constants of the binding and the calculated KD values The affinities of ... proteins in the presence or absence of Fn (or the NTD), all of the mAbs showed strong anti-LIBS activity However, these results not rule out the possibility that the immune system of the host might

Ngày tải lên: 06/03/2014, 22:21

... comprising the N-terminal fragment and the first AAA-cassette of PEX6 (PEX6N + D1–GAL4-BD). Neither of the cas- settes alone nor the two AAA-cassettes together or the N-terminal part together with the ... activation of the reporter gene and thus is suffi- cient to maintain the interaction with Pex1p. These findings demonstrate the importance of the first AAA- cassette together with the N-terminal region of ... required for the overall function of Pex1p in peroxisome biogenesis. This conclusion was based on the analysis of functional consequences of muta- tions of the conserved lysine in WalkerA of both AAA-cassettes

Ngày tải lên: 07/03/2014, 16:20

... Deletional analysis of the 5 ¢ end of the xMGP promoter reveale d a minimal activating ele- ment in the s equence from )70 to )36 bp. Synthetic reporter constructs containing three c opies of the d ... present the molecular organization of the first nonmammalian M GP gene and the functional analysis of its promoter. We identified a region within the first 70 bp of the xMGP promoter that mediates ... 2002 The understanding of the fine tuning of MGP gene expression requires further investigation and the use of different vertebrate systems may be useful in bringing new insights into the mat ter of

Ngày tải lên: 08/03/2014, 10:20

... features of the consensus motif into the Zm-p60.1 scaffold, an analysis of structural and catalytic properties of the mutants, and simulations of the substrate–enzyme interactions of the wild-type ... despite the substantial progress that has been made towards elucidating the mechanism of glucosidic bond cleavage and the roles of the catalytic pair, our knowledge of the molecular determinants of ... bioinformatic analysis of plant b-glucosidases to obtain insights into the evolution of the molecular sites involved in the two modes of aglycone binding The amino acid sequence of Zm-p60.1 was

Ngày tải lên: 16/03/2014, 04:20

... from the Trp residue, buried in the interior of the protein molecule, to the ionone ring of retinol would be expected. Inter- estingly, binding of retinol caused an increase of the emission of the ... cells of the inner hypodermis and the epithe- lium of the oviduct as well as extracellularly in the pseudocoelomic cavity of the worms. Discussion A bundle of four a-helices constitutes the secondary structure ... good correspondence of the experimentally determined pI of 6.1 and the theoretically deduced pI value of 6.22 was found. A theoretical prediction of the secondary structural organization of Ag-NPA-1

Ngày tải lên: 16/03/2014, 18:20

... estimated from Hanes plots. Determination of the metal contents of the Qor proteins, and detection of the nucleotide moiety of the molybdenum cofactor The contents of molybdenum and iron were determined ... catalyse the synthesis and insertion of a cytidine dinucleotide cofactor into recombinant Qor as suggested by the release of CMP after hydrolysis of the enzyme, it appears that the assembly of intact ... indicated in Fig. 4B. The absence of Mo(V)- signals reveals the g 2 -component of FeSII. It is noted that the g-factors of the FeSI and II signals of the Qor proteins from the wild-type strain

Ngày tải lên: 17/03/2014, 10:20

... members of the VOC superfamily. Superimposing P. shermanii MCE on the human glyoxalase structure shows that the Co 2+ ion of the MCE is only 0.2 A ˚ from the position of the Zn 2+ ion in the glyoxalase ... involved the insertion of several fragments of the 5¢-region of mce-1 into the mul- tiple cloning site of the vector pPD95.77 provided by A. Fire (Carnegie Institute, Baltimore, MD, USA). The inser- ted ... suggests the participation of two active site functional groups that act as acid ⁄ base catalysts in the epimerization reaction [21], wherein one base abstracts the C2 proton of the S-epimer of methylmalonyl-CoA,

Ngày tải lên: 23/03/2014, 13:20

... character- istic of the L4 stage. The arrow indicates turning of the gonad, which occurs at the early L4 larval stage (F). Wild-type worms growing on the 5-FU plates are arrested at the L2 stage ... 5¢-deoxy-5-fluorouridine (5dFUR) to further examine the function of UPP-1 in the pyrimidine bio- synthesis pathway. 5dFUR is converted to 5-FU by UP and TP [9]. The effects of 5dFUR in the upp-1 mutant are questionable, ... that knockdown of these two genes is very specific. To more precisely understand the functions of these uridine kinase homologs, we analyzed their enzyme activity in vitro. The proteins purified from the

Ngày tải lên: 30/03/2014, 01:20

... DU145 cells. (C) The effects of 2 lg of the NEG-U decoy, 2 lg of the NEG-D decoy and a combination of 1 lg of each decoy on the activity of F-560. The open ellipse indicates the wild-type NEG-U ... Nucleotide sequence of the 5¢-upstream region of the Pokemon gene. The upstream region of the Pokemon gene containing the pro- moter and a short stretch of the transcribed region is shown. The nucleotides ... POS-D. Importance of the POS-D element for the strong activity of the Pokemon promoter Because the POS-D element plays an important role in the strong activity of the Pokemon promoter, it may be the target of

Ngày tải lên: 30/03/2014, 04:20

... comparison of these cells has not yet been carried out, particularly in relation to their proteomes We therefore sought to run a comparative study of the proteomes and functional properties of these ... compared the proteomes of all three cell types In this study we compare the proteomes of mDCs, moDCs and KG-1 cells, and then attempt to relate this to the functional properties of these cells ... functions The functional relevance of differentially expressed proteins was confirmed by analysis of surface marker expression, cytokine profile, endocytic and T cell activation abilities of the cells,

Ngày tải lên: 14/08/2014, 18:20

... are used The former is concerned with the description of the main areas of functional grammar and the latter deals with the analysis of the text for discussion 1.5 Data collection The text is ... Introduction – presents the rationale of the study, the aims of the study, scope of the study, methods of the study, data collection, and the research design  Chapter two – Theoretical Background ... Analyzing the meaning and structure of President Nguyen Minh Triet’s speech addressed on the occasion of the 1000 th anniversary of the foundation of Thang Long – Hanoi based on the systemic functional

Ngày tải lên: 28/02/2015, 11:54

... listener; the Subject is the warranty of the exchange It is the element the speaker makes responsible for the validity of what he is saying (iii) The Theme functions in the structure of the CLAUSE ... cohesion The focus is on some issues relating to the analysis of the text My original writing of the thesis was based on the format and methods employed in the previous M.A Theses in the same ... Terms of Transitivity, Mood and Theme…… 12- 28 2.3.1 The Transitivity Pattern of the Text……………… 28-29 2.3.2 The Mood Pattern of the Text……………… 29 2.3.3 The Thematic Pattern of the

Ngày tải lên: 28/02/2015, 11:55

... using systemic functional grammar as the theoretical framework Scope of the Study Given the aim of study set above, in this thesis I would only focus on the analysis of a text in terms of transitivity, ... 2.3 The Context of the Chosen Text ……………………………………… 16 2.4 Clause and Clause Complex Analysis ………………………………… 17 2.5 The Analysis of the Text in Terms of Transitivity, Mood and Theme 19 2.5.1 The ... Students of Geography, Book Methods of the Study This study attempts to analyze the meaning and structure of a geography text Therefore, description and analysis are the main methods of the study The

Ngày tải lên: 02/03/2015, 14:22

... Morupule. The local mine Morupule Colliery supplies the coal for the power station. Most of the people living in Palapye are employed by either the power station or the colliery. The other groups of ... coping with the burden of orphans and providing support. The caregivers acknowledge that there are lots of OVC who are cared for by the government. As in Palapye, one of the strengths of the government ... take care of their own needs as well as the needs of the children with whom they live. The conditions under which OVC are raised indicate that most of them live in poverty. Indicators of poverty...

Ngày tải lên: 06/03/2014, 01:22

... envelope of the bound protein. The percentage of protein bound to RNA was the ratio between the value of bound protein and the total protein present in the sample. The relative percentage of binding ... evaluation of the available model on the interaction and cleavage of the RNA substrate and the Kid toxin is of interest in itself because it is the basis of important cellular roles of this toxin ... test the formation of a stable heterooctameric Kid 2 –Kis 2 –Kid 2 –Kis 2 complex on the parD promoter [9]. We further analysed the effects of the mutations on the co-regulatory activity of the...

Ngày tải lên: 16/03/2014, 03:20

... no. Q7QKF0) where the Asp235 is missing. In this latter case, the absence of the Asp235 can be explained in the light of the model of the inter- action of the substrate with the active site (vide ... such enzymes. The structural and functional roles of the residues involved in the substrate selection and binding are proposed through the analysis of the predicted interactions of the model active ... moiety tethered to the phosphopantetheinyl group and the free amino group of the downstream aminoacyl moiety, thus facilitating the translocation of the growing chain onto the next module. The structural...

Ngày tải lên: 23/03/2014, 13:20