... Averages and SD were calculated separatelyfor the numerator and the denominator of Eqn (5). Basedon the SD of the numerator and the denominator the SEM of qhwas computed, assuming statistical independence ... University of Groningen, The NetherlandsIntroductionLiving organisms have the option to regulate theirmolecular activities by altering expression of the cor-responding genes. For example, in the ... reactions contained5 lL of SYBR Green PCR Core Kit (Bioke, Leiden, The Netherlands), 3 pmol of each primer (Isogen, De Meern, The Netherlands or Biolegio, Nijmegen, The Netherlands) and 3 lL of cDNA...