M ifiiv DICH ovcwrmujm
KET QUA VA BAN LUAN
3.1.linh che lectin tur 5 giong trong (Cultivar) ciia loai dau cove
(Phaseolus veilgaris L.) va nghien ciru tinh chat cua chung.
3.1.1. Tinh che lectin tir 5 giong trong trong loai dau covẹ
Leclin tir hat cua 5 gid'ng trbng d£tu cove deu diroc tinh sach theo quy Irinh gid'ng nhau nhir da md la phan phuong phap nghien cmị Két qua linh ché lectin dirac Idni tdi bang do thi va bang dirdi day:
Cac sac ky đ qua trinh sac ky trao đi ion qua cdt DEAE - Trysacryl tron*^ linh ché leclin tir hat dau covẹ
AO 20 3 0
, oo(620tti*»;
40 so
Hinh 1 : Sac ky do qua cot DEAE - Trysacryl, che pham lectin tir hat dau
cove naụ Dem phot phat 0,2M, pH 8,0. Toe d6 chay 20ml giof. Th^ tfch m6i phan doan 2ml. Kfch IhírcJc col 1,2cm x 8,0 cm. Chiet rut leclin bang gradient NaCl 0 ^ IM.
• • • Hoal do lectin, HAA
Mat do quang, OD - nbng do protein cac phan doan
A 0 0 ( ^ ^ 0 M Ml j
Hinh 2 : Sac ky đ qua col DEAE - Trysacryl, ché pham lectin tir hat dau
cove than. Dem phot phat 0,2M, pH 8,0. Tdc do chay 20ml gid. The tfch mdi phan doan 2ml. Kfch thiJoc col 1,2cm x 8,0 cm. Day bang gradient NaCl 0 ^ IM.
Hoat do leclin, HAA -«—•-
-*- OD - Mai do quang, ndng do protein cac phan doan.
4 A A OS- ^HAA I /] * C - — #— I T , 1 - \o OS 9 5 Q 2 ô 4 0 1<> 5 0 4 0 5 0
Hinh 3 : Sac ky đ qua cot DEAE - Trysacryl, che pham leclin tijr hat dau
cove vang. Dem phot phat 0,2M, pH 8,0. Tdc đ chay 20ml gid. Thd tfch mdi phan doan 2ml. Kfch thirac cot 1,2cm x 8,0 cm. Day bang gradient NaCl 0 - IM.
• • • Hoat do leclin, HAA
OD - Mat đ quang, ndng do protein cac phan doan. •* — * -
A HAA
0 - 6
-IO S.O 30 40