M ifiiv DICH ovcwrmujm
Cd the ndi rang tfnh chai sinh hda ciia leclin lir loai dau guom va lir loai dau cove cd nhieu diém khac biet nhaụ
loai dau cove cd nhieu diém khac biet nhaụ
Neu nhu lectin cay dau cove kha ben vdi nhiet, van cdn hoat tfnh d 70"C sau 15 phiil thi d nhiet đ > 40°C leclin dau guom da mat hoal linh sau
15 phiil (anh hirdng ciia nhiel do mdi trirdng den hoal tfnh leclin dau guom
xem phu luc 1).
3.3.1.1. Anh hirdng ciia pH mdi trudng.
Dd pH thfch hgp ciia leclin dau cove kha rgng lir 6,0 - 8,0. Leclin tii dau guam đ pH thfch hgp ciia nd lai rai hep 7,0 - 7,5. Nhu vay dau guom nhay cam vdi pH mdi trudng hem leclin loai dau covẹ
3.3.1.3. Tuong tac dac hieu dirdng.
Ve diem nay, chiing cung khac biet nhau ro ret. Leclin tir loai dau cove khdng bj kim ham dac hieu bdi cac loai dudng. Hau hei cac loai dirdng glucoza, manoza va mgl sd dan xuai ciia chung deu kim ham hoat đ leclin loai dau covẹ Ngugc lai leclin lir loai dau guom chi bj lic che hoal đng bdi glucoza, manoza va mgl vai dSn xual amin cua nd. Nhu vay leclin lir loai dau guom tuong tac dac hieu vdi nhdm dirdng glucoza, manozạ
3.3.1.4. So sanh cau tao phan tir. 3.3.1.4.1. Khdi lugng phan tuf lectin. 3.3.1.4.1. Khdi lugng phan tuf lectin.
Xac djnh khdi lugng phan lii bang phuang phap dien di tren gel polyacrilamit theo ky Ihuat ciia Laemmli chiing tdi thay Mr ciia hai dang lectin tren hoan loan khac nhaụ Leclin tir loai dau cove gdm 2 lieu don vi khac nhau la 31 kDa va 34 kDạ Trong khi đ, leclin tir loai dau guom gbm cac tiéu dan vj gidng nhau va khdng lien kei vdi nhau bang lien kei disunfua (-S-S-), mdi tieu don vi 31 kDa va xuat hien bang protein 14 - 15 kDa do thiiy phan ndi sinh lir lieu dan vj 31 kDạ
Bang 11 : So sanh mdt sd tinh chat sinh hda ciia lectin tir hai loai khac
chi : Loai dau cove (phaseolus vulgaris L.) va loai dau gucmi (Canavalia
gladiatafacq.).
Tinh chat sinh hoa Do ben nhiet pH thich hop
Duong uc die
Khoi lugng phan tir ciia cac tieu doti vi
Phaseolus vux^lgaris L. <70°C 6.0 - 8.0 D - glucoza D - glucozamin N - Axelvlglucozamin D - manoza D - galactoza Gbm cac tieu don vi khac
nhau 31 va 34 kDa
Canavalia gladiata jacq.
<35°C 7.0 - 7.5 D- glucoza D - glucozamin N - Axel'viglucozamin D - manoza
G6m cac tieu don vi gidng nhau 31 kDa
3.1.4.2. So sanh thanh phan axit amin va trinh tir ciia 15 axit amin tan cimg dau N ciia lectin tir loai dau cove va tir loai dau girorm. cimg dau N ciia lectin tir loai dau cove va tir loai dau girorm.
De giai thfch dugc sir khac nhau can ban ve tfnh chai hda sinh ciia leclin tir hai loai dau Iren, chiing tdi da lien hanh nghien ciiu thanh phan axit amin va Irinh lu 15 axit amin tan cimg d'au N ciia leclin dau guom, tiép theo lien hanh so sanh vdi thanh phan axil amin va trinh tu ciia 15 axil amin tan cimg dau N ciia lectin dau cove than da dugc Hoffman va cdng sir cdng bd (1982) 163). Tir kei qua Ihu dugc d bang 12 chung tdi nhan thay : Ihanh phan va so lugng axil amin cua hai dang leclin nay hoan loan khac nhaụ
a - Ve sd lugng axfl amin trong phan tir : leclin lir dau guom cd sd lugng gdc axil amin la 236, trong khi leclin lir dau cove chi chiia 222 gd'c axit amin.
b - Néu xet v'e Ihanh ph^an axil amin thi chung luong lu nhau gdm 17 loai axil amin va khdng chiia mgl gd'c Xistein naọ Tuy vay khi xel chi liei sd lugng timg loai axfl amin thi giua chiing cd sir sai khac rd ret. Cac loai axil amin trung tfnh khdng tfch dien nhu Ala,Val, Gly, Leu, He cd sd lugng khac