... essential for the formation of all subcomplexes However, the disappearance ofa peak at 214 nm in the HPLC chromatogram does not reveal the extent ofthe damage to b-phycocyanin In fact, it may be that ... before and after illumination revealed the disappearance ofthe b-phycocyanin already after h of illumination (Fig 4) In contrast, SDS/PAGE analysis ofthe total mixture of phycobilisomes exposed to ... allowed a quantitative estimation ofthe relative amount of each protein component from the area underlying each peak This was facilitated by the fact thatthe biliproteins are strongly conserved and...
... involved in an intramolecular H-bond withthe b-galactose [20] and the lack of VT1, as opposed to mAb anti-Gb3 binding to this analogue may indicate that restriction of rotation around this anomeric ... low mass gate at 800 Da The mass spectra were externally calibrated withthe molecular mass ofa mixture of standard peptides Results Comparison of ligand/Gb3 binding by RELISA The binding of VT1, ... goat anti-rat IgM (1 : 1000), and StreptABComplex/ AP, in the same manner as above, and mAb 38.13 binding was visualized with HistoMark Red substrate After washing with water and 20 blocking with...
... separately in the region tothe “left” of ξ1 (that is, in the region AKJDE) and tothe “right” of ξ1 (that is, in the region ABCLK) In particular, it is not difficult to see thatthatthe number of ... addition, in that case the determinant in (2.3) is equal to because it is the determinant ofa triangular matrix with 1s on the antidiagonal This completes the proof ofthe lemma We are now in the position ... generality, we may suppose that d ≥ e We choose to place the matching X ∪m∪Y in Conjecture 1.2 in such a way that, again, the set of arches of X appear on the very right ofthe upper side of the...
... all-1 matrix Proof of Theorem 1.1: The square ofthe number of perfect matchings of G counts ordered pairs of such matchings We claim that this is the number of spanning 2-regular subgraphs H of ... tothe permanent) Thus the square ofthe number of perfect matchings is at most the permanent ofthe adjacency matrix, and the desired inequality follows from Bregman-Minc by taking the square ... came from M1 and which from M2 The permanent ofthe adjacency matrix Aof G also counts the number of spanning 2-regular subgraphs H of G, where now we allow odd cycles and cycles of length as...
... be associated witha favorable safety and tolerability profile As the improvement of adolescents with cannabis abuse disorders may be the result of general treatment factors [26], the use ofa ... Patient-report of depressive symptomatology was assessed withthe BDI [30] The 21 items are summed to obtain a total score that can range from to 63, with scores of 20 or more indicative of moderate to severe ... interval is greater than zero, this indicates a 95% or greater probability thatthe mean change associated with fluoxetine treatment is greater than the mean change associated with placebo dGeneralized...
... assigned on the basis of NOEs from the a- and b-protons of Val1 tothe amide proton of Cys2 The amide proton of Val1 disappeared in the H2O spectrum, possibly because of its special position at the N-terminus ... Pro4 was assigned by its TOCSY spin system and the NOEs from its a- proton tothe amide proton of Phe5, and from its d-proton tothe a- proton of Gly7 The valine residue at position was finally assigned ... IA, Gehrmann J, Loughnan ML, Thomas L, Adams DA, Atkins A, Palant E, Craik DJ, Adams DJ, 2602 Alewood PF et al (2001) Two new classes of conopeptides inhibit the alpha1-adrenoceptor and noradrenaline...
... 0 a (1 − α1 ) > − α1 and ® A ∪ Awith >a ≥ 0, β1 = a0 a ≥ a1 ≥ a − A1 ∪ A2 a β1 = > It is noted thatthe condition (3.1) is not guarana0 − α1 teed to be contractive for any point of A1 It is also ... k1 ofthe equality defining a circle Aa1 for a fixed a1 = a1 (x0, y0) ≤ a- a’ is defined below together with available point-dependent lower and upperbounds: De la Sen and Agarwal Fixed Point Theory ... since the associated (X, || ||) is a Banach space, z ∈ i∈p Ai is a unique fixed point of T q : i∈p Ai → i∈p Ai Thus, again the continuity ¯ ¯ ¯ of T q : ¯ i∈p Ai → ¯ i∈p Ai and the fact that it has...
... go off at any moment go off begin, start (used with signals, alarms, warning sounds) The alarm clock went off at 6:00 AM go off stop (said ofa machine) The DVD player goes off automatically if ... finally passed away at the age of 87 pass * off + try to convince someone that something is real He tried to pass the fake watch off as a real Rolex pass * on + transmit Please pass this message ... need to take some of my skirts tothe tailor to have them taken in take * off whena plane or rocket leaves the ground My stomach felt funny whenthe plane took off take * off + remove In many...
... arthritis All treated animals are illustrated in panels a and b; those witha clinical score of or less at the time of DNA injection (day 27) are illustrated in panels c and d; and animals witha clinical ... in Fig 4a and 4b The results indicate that there was no therapeutic effect of pcTNFR treatment The clinical score of mice at the time of DNA delivery on day 27 was in the range 0.54, and a published ... self-contained plasmid would reduce basal expression and increase the magnitude of regulation, as was recently achieved with an adenoviral vector [28] According to data obtained in clinical trials,...
... many other factors that limit the use of adoptive T-cell therapy for cancer For example, the failure of adoptive immuno therapy against cancers lies in the absence of tumorspecific sources of ... Cancer, Ovarian Cancer and Leukemia SPOREs, and by a 2009 Seena Magowitz Pancreatic Cancer Action Network AACR Pilot Grant Author details RNA interference and non-coding RNA Center and the Department ... laboratory Since July 2007 he has been an associate professor in Experimental Therapeutics at the MD Anderson Cancer Center and studies the roles of microRNAs and other non-coding RNAs in cancer...
... comparative dual channel approach, at a limited risk of increased false negatives but withthe added advantage of being able to identify the presence and absence of any gene on the microarray and ... coli pathogenicity All authors edited and approved the final manuscript Additional data files The following additional data are available withthe online version of this paper Additional data file ... assess the performance ofthe chip as well as to identify the best way of analyzing data from it, control sample hybridizations were analyzed Comparative hybridizations on dual channel microarrays...
... throughput assay to systematically interrogate genes of maximal interest at low cost [6,7] Boutique arrays are almost always two-color cDNA arrays because cDNA arrays are the easiest to customize and the ... Boutique arrays are custom-made arrays that may contain only a few score genes With such small arrays it is easy to step beyond the tolerance of lowess normalization, particularly as genes are often ... for normalization [12] The fact thatthe spike-in RNA is not extracted withthe main RNA sample and has to be added separately means thatthe spike-in probes will not always follow the same intensity-dependent...
... information is an important indicator ofthe stabilization of denitrification activity in the water treatment apparatus and will help withthe water treatment apparatus improvement Furthermore, the ... (in Japanese with English abstract) Miura A. , Shiratani E., Hamada K., Hitomi T., Yoshinaga I and Kubota T (2009) Removal of nutrients, organic matter and heavy metals from paddy field drainage ... (Hiraishi et al., 1996) The molar ratio of menaquinone to ubiquinone (MK/Q) is used as an indicator ofthe respiratory state such as the balance between anaerobic and aerobic respiration The Quinon...
... thatthe conformational space of b-amino acids is larger than thatof a- amino acids, but low-energy conformations ofthe b-amino acids backbone, corresponding to gauche rotamers around the Ca–Cb ... [b2-HAla9]SP and [b3-HAla9]SP may adopt conformations around residue that are analogous to those adopted by a- amino acids (Gly, Ala, Sar, Pro) To explain the slightly higher biological potency of ... in the sequence ofthe C-terminal heptapeptide of NKA, another peptide ofthe tachykinin family that binds the NK-1 and NK-2 receptors [HGly8] NKA(4–10) is as potent as NKA and [Ala8]NKA on rabbit...
... proposed thatthe WD protein acts as a copperspecific pump that mediates the export of copper from the cytosol, similar tothe P-type ATPase [14] On the basis ofthe fact that MREs mediate the transcriptional ... 5047–5052 Hoff, C.M & Jacob, S.T (1993) Characterization ofthe factor E1BF from a rat hepatoma that modulates ribosomal RNA gene transcription and its relationship tothe human Ku autoantigen Biochem ... Several studies have reported that MREa in the promoter ofthe MT gene possesses transcriptional regulatory activity and thata variety of nuclear transcription factors interact specifically with...
... G A G AAAAA AT T TA A G AT C T AT T T G AA C T A G A C C A AT G C T G G G A G AAAAA AT T TA A G AT C T Mut A AT T T G AA C T G T G AA G AT G C T G G G A G AAAAA AT T TA A G AT ... T Mut B AT T T G AA C T G T G C C A GGAC T G G G A G AAAAA AT T TA A G AT C T Mut C AT T T G AA C T G T G C C A AT G A G A G G A G AAAAA AT T TA A G AT C T Mut D AT T T G AA C T G ... C A AT G C T G AA G G AAAAA AT T TA A G AT C T Mut E AT T T G AA C T G T G C C A AT G C T G G G AA G G AAA AT T TA A G AT C T M u t F AT T T G AA C T G T G C C A AT G C T G G G A G A...
... basis of human IP cDNA with specific modifications The primer sequences used were 5¢-ATTCTCGAGATGGCGGAT TCGTGCAGGAAC-3¢ (forward) and 5¢-AAGAATTCA CAGGGTCAGCTTGAAATGTCAG-3¢ (reverse), with XhoI and ... (IP-wt) was approximately 2% ofthe total c.p.m added, whereas the binding of HEK293 cells expressing the same receptor reached up to 10% (Fig 2Ab) These data indicated thatthe expression level of ... have shown that after replacement of R173 with an Ala residue (R17 3A) , IP loses ligand-binding activity [16]; and (d) it has also been reported thatthe mutant with replacement of P179 by Ala...
... dLtA-P1, 5¢-ACAAATATAGACACCGAGCAAAATGG CAA; dLtA-P2, 5¢-CGAGCTCGAATTCGTAATCATGGT CATATTATAAATATATGAACCGCTATTCGCGGT-3¢ (3¢ kanamycin fragment underlined); dLtA-P3, 5¢-GTAT AATCTTACCTATCACCTCAAATGGTTCTCGTTTTTA ... A- domain witha d-aa as the sole substrate has been biochemically characterized Especially the fact thatthe enzyme does not activate l-Ala corroborates the finding that A- domains as well as aaRS ... used to exclude the possibility that may act as an inhibitor in pathways other than thatof DltA We also test the susceptibility of these strains toa combination of and vancomycin and observed as...