what organ and which hormones are responsible for maintaining normal blood glucose levels
Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx
... Research Board and Enter-prise Ireland. We are grateful to Dr Harinder Singh,Department Molecular Genetics and Cell Biology,University of Chicago for the gifts of pcDNA3:HaOct1 and pcDNA3:HaOct2.References1 ... Prm2 and Prm3, in addition to exon (E) 1, E1b and E2, which are illustratedabove each panel. Nucleotide +1 corresponds to the translational start site (ATG) and nucleotides 5Â of that site are ... expression A. T. Coyle and B. T. Kinsella1038 FEBS Journal 272 (2005) 1036–1053 ª 2005 FEBS tion and cells lysed and prepared for luciferase assays asdescribed above.HEK293 and HEL cell supernatants...
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Báo cáo khoa học: Two short protein domains are responsible for the nuclear localization of the mouse spermine oxidase l isoform pdf
... nuclear localization for the mSMOl isoform (Fig. 4).Taken together, these results consistently substanti-ate the hypothesis that these two structural regions are mandatory for the nuclear localization ... model from a given template and alignment[18].Expression of mSMOa, mSMOl and mSMOlDisoforms in E. coli cellsE. coli BL21 DE3 (Novagen) cells transformed with thepmSMOa and pmSMOl plasmids, as ... containing 20% (w ⁄ v)sucrose and 1 mm EDTA, and then incubated for 5–10 minat room temperature. Each suspension was centrifuged at10 000 g for 10 min at 4 °C and then the pellets were resus-pended...
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Tài liệu Investigation of Organic Chemicals Potentially Responsible for Mortality and Intersex in Fish of the North Fork of the Shenandoah River, Virginia, during Spring of 2007 ppt
... 2008–1093Prepared in cooperation with the Friends of the North Fork of the Shenandoah RiverInvestigation of Organic Chemicals Potentially Responsible for Mortality and Intersex in Fish of the North Fork ... North Fork of the Shenandoah River organization and their support-ers, including the Virginia Environmental Endowment, for pro-viding funding for this work. We also thank John Holmes and his ... spectrophotometrically (Routledge and Sumpter, 1996; Investigation of Organic Chemicals Potentially Responsible for Mortality and Intersex in Fish of the North Fork of the Shenandoah River, Virginia,...
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Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
... increase of 106-fold for dCyd. Vmaxwas fairlyunchanged compared with the wild-type. The Kmvalue for BVDU was increased approximately twofold com-pared that for the wild-type, which indicated ... identified and proposed as being responsible for substrate specificity [4], were mutagenized and themutant enzymes characterized for their ability to phos-phorylate native deoxyribonucleosides and ... analmost 2000-fold decrease in the kcatvalue for dThd, and a 270-fold decrease for dCyd. The Kmvalue wasincreased sevenfold for dThd and 49-fold for dCyd.The large decrease in kcatvalue...
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Tài liệu Báo cáo khoa học: "Which words are hard to recognize? Prosodic, lexical, and disfluency factors that increase ASR error rates" ppt
... Edinburgh-StanfordLINK and ONR MURI award N000140510388. Wethank Andreas Stolcke for providing the ASR out-put, language model, and forced alignments usedhere, and Raghunandan Kumaran and Katrin ... withShinozaki and Furui (2001) and Siegler and Stern(1995). The effects of pitch minimum and maximum are not shown for reasons of space, but are similarto pitch mean. Also not shown are intensity mini-mum ... fixed effects and a ran-dom effect for speaker identity. One model also con-tained a random effect for word identity. Results are shown in Table 3. The model without a random ef-fect for word identity...
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Báo cáo khoa học: Glutamic acid residues in the C-terminal extension of small heat shock protein 25 are critical for structural and functional integrity pptx
... residues inFig. 1) are tallied. Only the totals are given for murine sHsps. Residues that promote disorder are in bold and those that promote order are in italic [32]. Residues are denoted as charged ... polar segment which is unstructured and highly flexible and protrudes from the oligomeric structure. Both the polar-ity and flexibility of the C-terminal extension are important for the mainte-nance ... property that has also been observed recently for wild-type and mutant forms of aB-crystallin [60,61]. TheE190A and E204A mutants performed poorly comparedwith the wild-type protein in both assays,...
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Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
... fold are cycled via the calnexin-calreticulin-glucosidase I and II system within the ER lumen, providingspace and time for the unfolded/partially folded proteins toacquire the correct 3D conformation. ... plasmids encoding the correspondingGCPII variants and that the DNA was transcribed, weisolated genomic DNA and total RNA from the inducedcells and performed PCR or RT-PCR assays, respectively.The ... electroblotted onto a nitro-cellulose membrane, and immunostained as described in the Materials and methods. Relative band intensities were recorded using a CCDcamera, and the concentrations of individual...
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Báo cáo khoa học: Saccharomyces cerevisiae Ybr004c and its human homologue are required for addition of the second mannose during glycosylphosphatidylinositol precursor assembly ppt
... wasthe case for ybr004cD-pGAL-YBR004c cells which showed impaired growth compared to a wild-typestrain on medium containing glucose and 16 lg CFWper mL (Fig. 1A). Furthermore, glucose- grownybr004cD-pGAL-YBR004c ... embryonic development inmammalian cells [6], and for viability of the parasitesLeishmania mexicana [7] and the bloodstream form ofTrypanosoma brucei [8].GPIs are assembled in the membranes of the ... precursor assembly for which a responsible gene hasnot been discovered. A bioinformatics-based strategy identified the essentialSaccharomyces cerevisiae Ybr004c protein as a candidate for the secondGPI...
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Báo cáo khoa học: Sequences downstream of the transcription initiation site are important for proper initiation and regulation of mouse ribonucleotide reductase R2 gene transcription ppt
... TATAWtUTRmut and TATAmutUTRmut). We found that both TBP (Fig. 2A,compare lanes 4 and 5) and the R2 TATA-box (Fig. 2A,compare lanes 2 and 3) were absolutely required for transcription ... represent the intensity of each band normalized to theband in lane 1. The lower numbers represent the intensity of the bandsin lanes 5 and 6 normalized to the band in lane 4.1798 I. Kotova et ... asimportant for expression from different promoters, for example downstream promoter element (DPE) [14]. Wetherefore analyzed the sequence of the R2 promoter and the 5Â-UTR, and compared it to...
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Báo cáo Y học: Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf
... also are important constituents ofeubacterial cell walls [11]. They are found in microorga-nisms, plants and animals and their function and physio-logical roles have been investigated and identified ... theenvironment and the gene for the enzyme was cloned and then sequenced. The recombinant protein, N-D-AAase, wasalso produced in the Escherichia coli, purified and charac-terized.MATERIALS AND METHODSBacterial ... sterile toothpick and incubated at 37 °C overnight. The transformantswere screened for enzyme activity by adding to each well10 lL2mgặmL)1lysozyme and incubating at 37 °Cfor30 min. This was...
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Báo cáo khoa học: Pyruvate:ferredoxin oxidoreductase and bifunctional aldehyde–alcohol dehydrogenase are essential for energy metabolism under oxidative stress in Entamoeba histolytica pdf
... characterization was carried out onEhPFOR, which is required for a full description and understanding of amebal glycolysis and fermentationpathways.EhPFOR kinetic propertiesPFOR activity was obtained ... before O2exposure, which were1.05 0.11 Uặmg)1(n = 6), 0.075 0.033 Uặmg)1(n = 3),0.47 0.28 Uặmg)1(n = 4) and 0.176 Uặmg)1(n = 1) for PFOR,ALDH and ADH for EhADH2, and acetyl-CoA synthetase, ... shown).Modeling EhPFORA 3D model of EhPFOR was built by using theDesulfovibrio a fricanus PFOR (DaPFOR) tertiary s tructure0 60 120 180 240 300 360020406080100 PFOR PFOR + dithionite% EhPFOR activityMin0.00...
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Báo cáo khoa học: A characteristic Glu17 residue of pig carnitine palmitoyltransferase 1 is responsible for the low Km for carnitine and the low sensitivity to malonyl-CoA inhibition of the enzyme docx
... concentrations(from 0 to 15 lm for P50H, H50P, P128H, H128P,PigE17DCPT1B and HumanD17ECPT1B, and from 0 to500 lm for D 18PigCPT1B and D28PigCPT1B). The assaywas performed at 1 mm carnitine as standard. To analyzePigE17DCPT1B ... observedbetween pig and human (Asp17 for human and Glu17 for pig) might be responsible for the kinetic charac-teristics of both CPT1B enzymes. Consequently, wegenerated two single mutant (pig E17D and humanD17E) ... recombining pig and human CPT1Bsequences before (H50P and P50H) and after (H128P and P128H) TM1 and TM2 respectively (Fig. 2A).These chimeras had similar specific activities (Table 1) and were expressed...
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Báo cáo khoa học: The sulfur atoms of the substrate CoA and the catalytic cysteine are required for a productive mode of substrate binding in bacterial biosynthetic thiolase, a thioester-dependent enzyme doc
... SPP and its thioesters are known to be func-tional substrates for thiolase, but kinetic constantshave been reported only for AcAc-SPP [3]; for exam-ple, Km and kcat are 73 lm and 469 ⁄ s for ... thiolase at 25 °C. The values and error estimates are calculatedfrom separate measurements (three for the wild-type and two for C89A). Ka and Kd are the association and dissociation constants, ... binding tunnel: Ser247 and His156. Ser247is known to adopt two different conformations; in theunliganded form, it points away from the tunnel, and, in the liganded conformation, it points into...
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Get a Life, Not a Job: Do What You Love and Let Your Talents Work For You
... PROFESSIONALLY, AND FINANCIALLY REWARDING CAREER29 for friends for about 7 years—mostly for free or in exchange for a good bottle of wine and a dinner and had taken a lot of courses toward that career ... interests:ã What are you good at what do others say you are good at?ã How could I be paid and leverage my talents?ã Realistically, what would it take to do this ideal job?ã What are the paths ... jobs, and the increased desire for work-life balance, people are looking for more stability, greater fulfillment, and increased satisfaction from work. Are you?HAVE MULTIPLE CAREER ACTS AND...
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