... phosphate [15] According to its sequence, PhyK belongs to a group of phytases synthesized by plant- associated bacteria such as Xanthomonas campestris, Pseudomonas syringae and Erwinia carotovora ... ofthe substrate-free PhyK with substrate-free ˚ AppA the Ca atoms are 2.41 A apart, whereas for the substrate-free PhyK and the substrate-loaded AppA ˚ the averaged distance is only 1.87 A Distinct ... whereas AppA undergoes a distinct conformational change upon substrate binding Classification of HAPs Induced conformational changes upon substrate binding For a detailed structural superposition, lsqman...
... is now also available; there is also a nice associated audit size calculation utility ona web site [7] Stanislevic [11] also examines the issue of choosing a sufficient audit size; he gives a ... situation; the math is the same We assume that we are in an adversarial situation, where an adversary may have corrupted some ofthe objects For example, the adversary might have tampered with the ... replacement, instead of sampling with replacement, so a sample size derived by assuming sampling with replacement is going to be an overestimate—in some cases a serious overestimate Nonetheless the...
... 5¢-GAGCTGAATCATTGTAACTTTAACTTC-3¢ 5¢-CAACATTGATGTCTTCAACAATG-3¢ 5¢-GCTGCAACATTGATGAATTCAACAATGTAAAG-3¢ 5¢-CTGCAACATTAGCGTTTTCAACAATG-3¢ 5¢-CATACGGAGCATGAGGGTTTCCTTG-3¢ 5¢-CGGGATCGCCATTTCGAGACGGAGGG-3¢ ... efficiencies than CA with the modified proteins, were analysed in catalytic assays Metabolism of alternate substrates The metabolism of CA analogues was assayed with the N302, I371 and K484 mutants (Table ... I37 1A I371K K484M 5¢-GAGTTTGGTTCGATGACAAGGAATGTTG-3¢ 5¢-GTTCGAGAACAATGAATGTTGTGTTC-3¢ 5¢-GAACACAACATTCTCTGTTCTCGAACC-3¢ 5¢-GAAGTTAAAGATACAATGATTCAGCTC 5¢-CATTGTTGAAGACATCAATGTTG-3¢ 5¢-CTTTACATTGTTGAATTCATCAATGTTGCAGC-3¢...
... being a historical representation, and Shakespeare play, was worthy of note 'The success of Henry V challenges the assumption [ ] that documentary style realism is the sole criterion of propaganda ... loved ones and the very real temptation of extra-marital affairs Whilst the Ministry of Information were not unaware of these issues they wanted to maintain an image of strength These problems could ... individual possessed The situations presented were, at last, 'real' and not distortions ofthe truth as had been previously depicted The characters had a sense of power and ability despite their background...
... templates for nanoscale conduits and networks Science 1996, 273:933-935 Karlsson A, Karlsson R, Karlsson M, Cans A, Strömberg A, Ryttsén F, Orwar O: Networks of nanotubes and containers Nature ... junctions by simple geometrical shapes and express the free elastic energy ofthe membrane in terms ofthe length ofthe LNT, its radius, the radius ofthe junction and the tension ofthe membrane Variation ... theory, and applications Biophys J 2005, 88:2714-2726 15 Karlsson M, Sott K, Cans A, Karlsson A, Karlsson R, Orwar O: Micropipetteassisted formation of microscopic networks of unilamellar lipid bilayer...
... is an elaboration on her/his suggestions 1.3 Models of Random Graphs: From k-regular Multi–graphs to Permutations Most work on random regular graphs is based onthe so called random configuration ... detailed account of these results, history and related work see the surveys of Aldous and Diaconis [AD99] and Stanley [Sta02] 1.1 Main Results In this article we establish combinatorial identities which ... For example, if one allows them to be weakly increasing This is equivalent to asking for the distribution ofthesizeofa largest planar subgraph, i.e the largest set of non–crossing edges which...
... purposes these calculations are considerably shorter as the oppositeness relation can be directly associated with the dual polar graph The dual polar graph is distance-regular and hence we readily ... Bose and T Shimamoto Classification and analysis of partially balanced incomplete block designs with two associate classes J Amer Statist Assoc., 47:151– 184, 1952 [4] A E Brouwer, A M Cohen, and ... In particular, the valency of i the oppositeness graph Γd+1 is q (d+1)(d+2+2ǫ)/2 Calculation ofa specific subset of eigenvalues ofthe association scheme The eigenvalues ofthe dual polar graph...
... databases, and 2) International Medication System (IMS) Because Turkey is an inflation country we have escalated all antibiotic prices The cost of antibiotics was calculated as US dollars (USD) ... consumption calculated by two-tailed Spearman s coefficient (r) for non-parametric correlations A P value of less than 0.05 was regarded as significant Software package STATA 9.0 (USA) was used for the ... many ofthe restricted antibiotics was decreased and the trend of resistance rates became downwards after implementation of NARP The amount of money saved increased further After two years of...
... measurements the concentration of pro-protease has been used in the calculations because once the proteases are autocatalytically activated as described above, it is difficult to isolate the active ... domain and is > A when the extension is on its own The fluctuation in the radius of gyration was also analyzed (Fig 6B) as a measurement ofthe overall stability ofthe CT-extension for both forms These ... intervals for 10 each and then immediately chilled on ice The activity ofthe proteases was measured at 65 °C (optimum temperature of activity for the proteases) against azocasein as substrate, as...
... and probably concentrated along bottomlands and terraces adjacent to streams (Sassaman et al 1990; Sassaman 1993) Sustained seasonal habitation ofthe area began between 9,800 and 8,000 years ... ofthe area The SRS manages its forests ona far longer rotation length than most managed lands in the Southeast Thus, the vast majority ofthe land area of SRS has suffered relatively minimal ... Terrestrial snakes associated with xeric upland habitats and mesic floodplain habitats onthe Savannah River Site 212 Figure 4.19 Aquatic snakes associated with stream systems and Carolina bays on...
... interpersonal skills, information provision, and availability; the nurses’ technical skills, interpersonal skills, information provision, and availability; the other hospital staff s interpersonal skills ... local situation as was suggested by Ganz [19] A subgroup of gynecologists, oncology nurses, a radiotherapist, a medical oncologist, as well as a general practitioner and a few patients adjusted ... participants onthe other hand are unaware ofthe group assignment, as they are under the assumption that the hospital is providing usual care Intervention versus usual care Usual care: The health care provider...
... Aspergillus niger and A awamori prefer longer malt-oligosaccharides as substrates, which is also the case for S fibuligera glucoamylases [15] The determination ofthe crystal structure of recom˚ binant ... in the ability to digest raw starch While Glu adsorbs to, but does not digest raw starch, Glm adsorbs well to starch granules and is capable of raw starch digestion The glucoamylases from Aspergillus ... al site, one acting as general acid and the other as general base [24] The mechanism of hydrolysis consisting of three steps involves proton transfer to the glycosidic oxygen ofthe scissile bond...
... c-glutamyl hydroxamate as standard Control assays were performed in the absence of ATP to ensure that the reaction was dependent on ATP Nitrate reductase (NR) was assayed in a total volume of 100 ... (Table 3) These findings suggest that the NR/GS inhibitor functions during both the assay preparation and the assays by converting the cofactors necessary for the NR or GS reactions into AMP Consistent ... percent ofthe amount of NADH used in our standard NR assay, and 60% ofthe amount of ATP in a GS assay, was converted into AMP within 10 at 30 °C by an amount ofa Mono Q fraction that appeared...
... the Swiss Alps I have seen few landscapes which so insistently preach the gospel of becoming as the mountains of Guanabara Bay Just as some pictures are so vividly painted as to give the spectator ... clouds the slanting rays ofthe sun ; pour down upon sea and coast The city yonder takes plastic shape as we look above the house-fronts rises a forest of towers and steeples to the right gleams the ... by the much smaller State of Espirito Santo Voyaging along the coast, one enters the new State as through an inlet ; for abreast ofthe frontier waters oftheof Bahia and Espirito Santo the Abrolho...
... NPR -A also appears to regulate fatty acid mobilization [5] NPR -A is a phosphoprotein that contains 1029 amino acids and migrates as a band of % 125 kDa molecular mass under reducing SDS ⁄ PAGE ... undergoes desensitization, which correlates with a loss of phosphate content in the KHD It is suggested that dephosphorylation ofthe KHD by distinct protein phosphatases [17] occurs as a consequence ... log-transforms for all experiments Finally, antilogs ofthe corrected log transforms were obtained and used for further testing Statistical analysis was performed by analysis of variance (anova),...
... CGGTAAGCCCATAACGCCCA CAGGCCAGGATTTGCAGCC CATAAACAYGAGCCAGTTGCC GAGTGGATGCACAGTCGTTG GAAACGGAGGTAGTGACACAT GCCTGCTCGAATTCGGGATG CTCCTTCTTGCACAAAAAGTG CTGCTCGAATTCGGGATG GTCYGGGTAATTCCTATATA GTGATCGAATTTGGGAAGATGATCCA ... also a single nonsense mutation leading to insertion ofthe TAA stop codon in exon of one ofthe vaspin A chain genes of V a zinnikeri (data not shown) These four clones are inactive The existence ... intron D similar to that ofthe V am ammodytes ammodytin I1 gene All PLA2 genes contained a TAA stop codon, an AATAAA polyadenylation site 80 bp downstream from the stop codon, and a TATA-like...
... IL, USA) was used for all statistical analyses A P value < 0.05 was considered to be statistically significant The results are reported as mean ± SD values Table 1: Baseline characteristics of ... terminated if participants developed severe dyspnea, dizziness, or chest pain, or had an abnormal heart rate response Expired gases were analysed every seconds using an automated online gas analyser ... cardiovascular risk profile, while assessing whether it remains successful at ensuring exercise adherence It appears that supervision and progression ofthe exercise programme may influence adherence...
... this variant The etiology of axial torsion of MD remains unclear Onthe basis ofthe available literature, we have identified several risk factors Although primary neoplasms arising within MD is ... with a normal appearance ofthe rest ofthe bowel and viscera The twisted Meckel s diverticulum was resected along with an cm flange of ileum Discussion This case report presents the unusual case ... within MD, such as lipomas, have also been recognized as a potential cause of torsion [12] Complications associated with this presentation include intussusception, with the tumor as the lead point,...
... although laparoscopy was shown to havea diagnostic accuracy of 96.9%, a significant number of cases still required conversion for their subsequent management Based onthe above literature and the ... terminal ileum and the anastomosis over sewn with continuous 3/ sutures The small bowel was then decompressed and the content milked gently into the stomach before being aspirated via the nasogastric ... Suffolk Hospital with a 3-day history of abdominal pain, vomiting, absolute constipation and abdominal distension The abdominal pain initially started as a dull generalised discomfort, but later...
... data are presented as means ± standard deviations All statistical procedures were analyzed using SPSS (Statistical Package for Social Science) version 16.0 Results Medical Monitoring, Dietary Analysis, ... increased in cross sectional area, the possibility exists that a strength increase accompanied this adaptation, even though there were no strength measurements assessed in this study The results ... cross sectional area of muscle fibers There is a direct relationship between a muscle s cross sectional area and overall strength of that particular muscle [40] Therefore, if the levator ani muscle...