vitro in vivo scaling of drug interactions
In vivo aspects of potential stereospecific drug interactions of oral warfarin and rutin in rats
... protein binding affinity differs between the two enantiomers of warfarin The S- isomer has a greater affinity to protein binding in man compared to the R- isomer Some of the drug interactions of ... such interactions are yet to be established (1) New drug products are routinely examined in vitro and in vivo for the potential to cause drug interactions via inhibition of drug- metabolizing enzymes ... helps in understanding the source of variation and mechanism of interaction of drugs with warfarin A better understanding of the warfarin drug interactions could be possible by either giving each...
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Stability and in vivo evaluation of pullulan acetate as a drug nanocarrier
... were fixed in 10% phosphate buffered formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E) In vivo pharmacokinetics and bioavailability In vivo pharmacokinetic study ... i.v at a dose of 200 mg/kg In vivo pharmacokinetics The plasma levels of EPI were determined following a single i.v injection of EPI or PA/EPI (10 mg/kg EPI equiv.) in female Wistar rats The plasma ... 6. Plasma drug concentration of EPI and PA/EPI after i.v injection in rats at a single equivalent dose of 10 mg/kg Table 5. Pharmacokinetic parameters of EPI and PA/EPI i.v injected in rats at...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... disrupting protein–protein interactions within PSI, or by altering the conformation of the loop His-tags were employed in a topological study of the major light harvesting chlorophyll a ⁄ b binding ... shown in Fig 4C Interestingly, both tags are recognized more efficiently in the context of the PSI-G loop region than at the C-terminus Topology of PSI-G in vivo In order to examine the topology of ... altered protein–protein interactions, which may explain why the Chlamydomonas loop region is exposed to trypsin in the in vitro assay The in vitro import experiments suggested that the loop of Arabidopsis...
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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
... upregulate expression of PD-L1, iNOS, and COX-2 in MSCs, making these molecules candidate mediators of T-cell inhibition The involvement of iNOS and COX-2 in inhibition of T-cell proliferation ... addition of inhibitors of these enzymes - GW274150 and indomethacin [8,36], respectively - to the co-cultures The addition of these inhibitors resulted in the abrogation of the inhibition of Tcell ... effect of MSCs on the disease course of CIA, mice were injected intravenously or intraperitoneally with × 106 MSCs in 100 μl of sterile PBS at the indicated time points Controls received injections...
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Báo cáo khoa học: "Comparative study on the in vitro and in vivo properties of two bovine herpesvirus-5 reference strains" pptx
... having an impact on the in vitro behavior of both BoHV-5 strains Concerning the in vivo characterization, in spite of the in vitro differences discussed above, we observed that both BoHV-5 strains ... BoHV-5 strain was isolated from a brain sample after several intracerebral inoculations of calves with brain tissue obtained from an outbreak of meningoencephalitis in calves reported in 1962 in Australia ... strains induced a similar degree of virulence in cattle Regarding the in vitro characterization, the results obtained from the one-step growth kinetic showed that the production of total infectious...
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Báo cáo y học: "In vitro and in vivo evaluation of a new active heat moisture exchange" potx
... the offset was maintained, with no significant variations We used this offset (in the order of 0.1–0.2°C) to correct the measurements obtained during the study In each condition, the average of ... is increased [16,30], causing an increase in carbon dioxide levels, especially during low tidal volume ventilation [31] HMEs also cause an increase the inspiratory work of breathing, with an increase ... 30 mgH2O/l) when using HMEs in patients with high minute ventilation (between 10.5 and 16.5 l/min) [19,23,24] In the present study we found that increasing or decreasing the minute ventilation...
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Predicting in vivo anti hepatofibrotic drug efficacy based on in vitro high content analysis
... 95], tracking of proteome sub-cellular localization [96], study of protein-protein interactions, and drug screening [97, 98] In order to interpret information captured in images obtained from ... combining all data into a single index for ranking We proposed an innovative method for determining the optimized weights for each of the 10 markers of fibrosis in Chapter 1.5.4 In vitro- in vivo correlation ... describe the extent of changes in fibrotic markers from in vitro culture 3.3.3 An in vivo anti-fibrotic drug efficacy index ranks drugs based on their in vivo effects 3.3.4 An in vitro efficacy predictor...
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In vitro and in vivo study of ABT 869 in treatment acute myeloid leukemia (AML) alone or in combination with chemotherapy or HDAC inhibitors insight into molecular mechanism and biologic characterization
... Phosphoinositide 3-kinase Promyelocytic Leukemia Zinc Finger protein Phosphatase of Regenerating Liver Retinoblastoma Protein Red Fluorescence Protein RNA interference Receptor Tyrosine Kinase ... Shock Protein 90 Indirubin Derivative Interferon gamma-inducible gene 16 Immunohistochemistry Intraperitoneally Internal Tandem Duplication c-Jun N-terminal Kinase Kinase Insert Domain Receptor ... Nanjing, M.sc National University of Singapore) A THESIS SUBMITTED FOR THE DEGREE OF DORCTOR OF PHILOSOPHY DEPARTMENT OF MEDICINE YONG LOO LIN SCHOOL OF MEDICINE NATIONAL UNIVERSITY OF SINGAPORE...
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In vitro and in vivo assessments of PCL TCP composites for bone tissue engineering
... a heparin binding site in N-terminal region of mature BMP-2 polypeptide In pioneering work by Ruppert in 1996, it was shown that BMP-2 activity was increased upon interactions with heparins present ... which participate in bone repair is of utmost importance Bone is composed of around 70-90% of minerals with the rest in the form of proteins Within the proteins in bone, the ratio of collagenous ... site Bioactivity of eluted BMP-2 maintained 19 2.2.6 Heparin The discovery of heparin occurred in John Hopkins University in 1916 While researching on the cause of blood clotting, Jay McLean accidentally...
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In vitro and in vivo characterisation of recombinant lactobacilli expressing house dust mite allergen
... IN VITRO AND IN VIVO CHARACTERIZATION OF RECOMBINANT LACTOBACILLI EXPRESSING HOUSE DUST MITE ALLERGEN LIEW LEE MEI 2009 IN VITRO AND IN VIVO CHARACTERIZATION OF RECOMBINANT LACTOBACILLI ... evaluation of in vivo 101 immunogenicity of the Blo t expressed in recombinant lactobacilli Figure 4.2 Oral feeding of recombinant Lactobacillus plantarum NC8 104 induced the production of Blo t ... of cytokines in splenic cultures Figure 4.5 Oral feeding of recombinant Lactobacillus rhamnosus GG 108 induced the Blo t 5-specific IgG2c production in mice Figure 4.6 Oral feeding of recombinant...
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In vitro and in vivo evaluation of customized polycaprolactone tricalcium phosphate scaffolds for bone tissue engineering
... degree of Master of Engineering in the Department of Mechanical Engineering at the National University of Singapore under the supervision of Professor Teoh Swee Hin and Dr Alvin Yeo No part of this ... CHAPTER 4: OPTIMIZATION OF NATIVE AND CUSTOMIZED SCAFFOLDS IN VITRO AND THEIR EFFECTS IN INITIAL BONE HEALING 4.1 INTRODUCTION 44 4.1.1 In vitro degradation study 44 4.1.2 In vivo degradation study ... coined as “bone tissue engineering” A key component in tissue engineering for bone regeneration is the scaffold that serves as a 3D template for initial cell interactions and the formation of...
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In vitro and in vivo evaluation of transferrin conjugated lipid shell and polymer core nanoparticles for targeted delivery of docetaxel
... anticancer drug delivery is the high plasma protein binding effect happening once the drugs enter the physiological system Plasma protein binding is part of the opsonisation process The opsonin proteins ... protein binding after IV administration (Extra et al., 1993) Docetaxel is mainly bound to alpha acid glycoprotein, lipoproteins, and albumin Among them, alpha acid glycoprotein is the main determinant ... THE DEGREE OF MASTER OF ENGINEERING DEPARTMENT OF CHEMICAL AND BIOMOLECULAR ENGINEERING NATIONAL UNIVERSITY OF SINGAPORE 2011 Acknowledgements First of all, I would like to express my profound gratitude...
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In vitro and in vivo investigation of nanoparticles of a novel copolymer for substained and controlled delivery of docetaxel
... as vindesine and vinorelbine can inhibit the assembly of microtubule by reducing the rate of tubulin addition, taxanes have the opposite effect, inhibiting the disassembly of microtubules during ... the drugs can be distributed to other parts of the body The most common kind of enzyme involved in this degradation of drugs is cytochrome P450 (or CYP), mainly located in liver and intestine ... used in clinical treatment of Parkinson’s disease which is characterized by severe depletion of neurotransmitter dopamine in brain Unlike dopamine, levodopa is capable of crossing the blood-brain...
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In vitro and in vivo study of vitamin e TPGs coated immunoliposomes for sustained and targeted delivery of docetaxel
... parts of the body The most common kind of enzyme involved in this degradation of drugs is cytochrome P450 (or CYP), mainly located in liver and intestine CYP is a large family of hemoproteins which ... as vindesine and vinorelbine can inhibit the assembly of microtubule by reducing the rate of tubulin addition, taxanes have the opposite effect, inhibiting the disassembly of microtubules during ... of tubulin in the microtubules rather than to tubulin dimers The binding site to tubulin differs from the one of vinca-alkaloids and podophyllotoxins While vincas inhibit polymerization and increase...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... are in agreement with those of a recent work in which GatY has been identified by other means as an in vivo substrate of GroEL [27] Interestingly, GatY is also included in a list of proteins that ... Services Department of the Weizmann Institute by a single injection of 150 lg of the purified protein, followed by two booster shots of the same amount of protein at 2-week intervals Serum was ... protein family [15] E coli contains five small members of this family including UspA itself (Fig 2), and one larger protein consisting of two UspA domains in tandem [17] The small members of this...
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Báo cáo khoa học: In vivo production of catalase containing haem analogues pot
... spectrum of the porphyrin contained in Zn-PP-KatA, in a pyridine ⁄ water ⁄ NaOH ⁄ Tween-80 mixture, was not similar to that of the reference solution of Zn-PP in the same solvent, indicating that ... the last step in haem synthesis, i.e the insertion of ferrous iron into PP As growth of bacteria in the Cofactor-substituted catalase presence of PP did not result in catalase containing Fe-PP, ... protein, in binding porphyrins It is not known how haem is transported intracellularly in bacteria, and the mechanisms by which haem is incorporated into soluble and membrane-bound proteins inside...
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Báo cáo khoa học: A steady-state modeling approach to validate an in vivo mechanism of the GAL regulatory network in Saccharomyces cerevisiae ppt
... reflected by the sequence of interactions; (c) parameters quantifying strength of interactions; and (d) spatial localization of protein in a compartment or shuttling of proteins between compartments ... sequences of the GAL genes through its N-terminal DNA binding site Gal80p inhibits the transcriptional activity of Gal4p by binding to its 28 amino acid region at the carboxyl terminal [25–27] In vitro ... enumerated various interactions resulting in a complex regulatory network Most of such studies demonstrate the in vitro interaction between the various components However, these in vitro experiments...
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Báo cáo khoa học: A mouse model for in vivo tracking of the major dust mite allergen Der p 2 after inhalation docx
... selenocysteine residue in a C-terminal Sel-tag [18] Der p carrying the Sel-tag had an intact core sequence and maintained allergen-specific IgE-binding epitopes and the use of a Sel-tag enabled labelling ... difficult to follow the in vivo clearance and turnover of an allergen after inhalation In this study we used a novel approach for specific labelling of proteins in order to investigate how an airborne ... deposited in the airways of mice and metabolized The labelling method involves the incorporation of a selenocysteine residue and the gamma-emitter selenium-75 (75Se) within an engineered C-terminal...
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Báo cáo khoa học: In vivo studies of altered expression patterns of p53 and proliferative control genes in chronic vitamin A deficiency and hypervitaminosis pot
... protein extracts were obtained as described in experimental procedures A single band of about 53 kDa was detected in liver and lung indicating that the amount of p53 protein was significantly increased ... role of vitamin A status in the expression of genes related to the regulation of cell proliferation has been studied in vivo and in vitro, and shows that retinoids play an important role in proliferation ... reproduced in lung, which can explain in part the conflicting results found in adults and children given b-carotene or vitamin A [29] Moreover, in lung of rats injected with high-dose of vitamin A over...
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Báo cáo Y học: In vivo activation of plasma membrane H+-ATPase hydrolytic activity by complex lipid-bound unsaturated fatty acids in Ustilago maydis docx
... observed, and this was independent of the kind of genetic lesion or inhibitor used, suggesting that this activating effect was indeed caused by the fatty acid/lipid environment of the ATPase It must ... and inhibitors acting on the same biosynthetic points has proved to be a useful way of distinguishing the in uence of direct effects, i.e sterol alteration, and indirect effects of EBI compounds ... cerevisiae, grown in the presence of octanoic acid [37] In this case, only Vmax was affected showing an increase that was accompanied by an increase in the presence of oleic acid in its plasma membrane...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose