vegetative cells endospores within cell and free endospores of bacillus subtilis mbi600 rif on endospore forming medium incubated for 8 days at room temperature 945 x
báo cáo hóa học:" Proliferating and differentiating effects of three different growth factors on pluripotent mesenchymal cells and osteoblast like cells" pdf
... methanol for 10 After rinsing with water cells were incubated for 30 at room temperature (RT) with 3% silver nitrate and for in formaldehyde Surplus silver nitrate was removed by incubation in 5% ... enhanced the in vitro bone formation synergistically [40] Therefore, for optimal stimulation of bone repair the controlled and local delivery of factors and factor combinations is mandatory [14,41,42] ... Cirri et al demonstrated the inhibition of PDGF induced cell proliferation after application of insulin [26] The simultaneous application of IGF-I, TGF-β1 and PDGF to osteoblast like cells enhanced...
- 8
- 434
- 0
Báo cáo y học: " Double rupture of interventricular septum and free wall of the left ventricle, as a mechanical complication of acute myocardial infarction: a case report" pot
... junctional, located at the junction between IVS and free wall [13] The analysis of similar cases has shown that the coexistence of FWR is frequently established only at the time of operation for ... hypertension (60%), and male sex (male/female ratio :8/ 2) were risk factors for the development of this complication [1] There are two forms of DMR: a) true, with rupture of both IVS and LV free wall, and ... days later, the patient was admitted to our Department for the scheduled coronary arteriography She reported mild exertional dyspnea and fatigue until about days ago On examination the patient...
- 5
- 280
- 0
Tài liệu Báo cáo khoa học: Secondary substrate binding strongly affects activity and binding affinity of Bacillus subtilis and Aspergillus niger GH11 xylanases docx
... Enzyme concentration (×10 8 M) LEGEND XBS wild-type XBS W 185 A XBS G56A-T 183 A-W 185 A 10 20 30 40 Enzyme concentration (×10–9 M) D 40 0 OSX solubilisation (% of OSX) OSX solubilisation (% of OSX) B ... subtraction of the control value (no enzyme) under the conditions of the assay and the assumption that no substrate exhaustion occurs Solubilization of water-unextractable AX and OSX Water-unextractable ... subtraction of the control value (no enzyme) under the conditions of the assay Activity on Azo-wheat AX An appropriate enzyme dilution and liquid Azo-wheat AX substrate were pre -incubated separately for...
- 14
- 600
- 0
Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc
... mevalonate pathway in conjunction with type II isomerase (62 out of 283 ) constitute the largest sets within the prokaroytic kingdom (Fig 8) The combination of the deoxyxylulose phosphate pathway ... T., Watanabe, H & Seto, H (19 98) A 1-deoxy-D-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-D-erythritol 4-phosphate in an alternative nonmevalonate pathway for terpenoid ... classical mevalonate pathway and the more recently discovered nonmevalonate pathway via deoxyxylulose phosphate (Fig 1) Of the 16 possible combinations, 10 were actually found in the group of 283 completed...
- 12
- 692
- 0
Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx
... ATP, at a physiological concentration, negatively affected GS binding to the TnrA sensor surface At a concentration of 2.5 mm, it decreased complex formation by approximately 60%, and at a concentration ... compilation ª 2011 FEBS 1 781 Interaction of TnrA with GlnK and GS A Kayumov et al Fig BIAcore analysis of GlnK–TnrA complex formation and ATP effect on dissociation of the GlnK–TnrA complex The ... (I) At late exponential growth phase, cells were washed and shifted to combined nitrogen -free medium, incubated at 37 °C with shaking for 10 min, and then harvested (II) The crude cell extracts...
- 11
- 596
- 0
Báo cáo khóa học: Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding pdf
... I), one oxygen atom of the ˚ carboxylic group formed a hydrogen bond (2.90 A) with bTyr 68 On the other hand, one carboxylic oxygen atom of another state (type II) appeared to be positioned at ... oxidative conditions, if the site contains no metal ion The metal activator of NHase may incorporate a metal ion to prevent the formation of a disulfide bond, and may facilitate the correct oxidative ... 7.6) From a cell- free extract prepared by sonication, the protein was purified by ammonium sulfate precipitation (40–70%) and anion-exchange chromatographies (DEAE–Sepharose and MonoQ columns)...
- 10
- 510
- 0
Báo cáo khoa học: N-Terminal segment of potato virus X coat protein subunits is glycosylated and mediates formation of a bound water shell on the virion surface docx
... corresponds to superposition of several absorption peaks Here a complex band of valent water OH-bond vibrations is overlapped with peaks of valent OH-bond and NH-bond vibrations of PVX CP Thus, ... originate from the virion ends, where the subunit conformation may be unsuitable for glycosylation What is the importance of this CP N-terminal glycosylation for PVX virion structure and function? ... although data on the effects of glycosylation on the conformation and dynamics of O-linked glycoproteins are accumulating [ 28 30] First, we were interested in the presence of glycosyl modification(s)...
- 10
- 398
- 0
Báo cáo khoa học: Quantitative interdependence of coeffectors, CcpA and cre in carbon catabolite regulation of Bacillus subtilis pot
... to increased complex formation of HPrSerP (Fig 3) Titration with rising concentrations of up to 40 mm of FBP or Glc6-P did not yield saturation (Fig 3B and D) The stimulation of HPrSerP binding ... CrhP at a fixed concentration of 10 nm of CcpA (Fig 5A and B) The results demonstrate that 50 lm HPrSerP leads to 2570 Fig HPrSerP and CrhP concentration dependence of the CcpA– xylAcre association ... carbon catabolite repression of the hut operon in Bacillus subtilis J Bacteriol 181 , 288 3– 288 8 Warner JB & Lolkema JS (2003) A Crh-specific function in carbon catabolite repression in Bacillus subtilis, ...
- 12
- 278
- 0
Care and compassion? Report of the Health Service Ombudsman on ten investigations into NHS care of older people pot
... compassion and professionalism The reasonable expectation that an older person or their family may have of dignified, pain -free These stories illuminate the gulf between the principles and values of ... be treated with respect, dignity caring for people with multiple and complex issues, and compassion disabilities and long‑term conditions and offering palliative care to people at the end of their ... dosage and contraindication) was that NSAIDs should be used with caution in elderly patients and that a proton‑pump inhibitor may be considered for protection against NSAID-associated gastric and...
- 44
- 430
- 0
Báo cáo khoa học: Characterization of L-aspartate oxidase and quinolinate synthase from Bacillus subtilis potx
... Chem 2 78, 88 04– 88 08 Ceciliani F, Caramori T, Ronchi S, Tedeschi G, Mortarino M & Galizzi G (2000) Cloning, overexpression and purification of E coli quinolinate synthetase Protein Expr Purif 18, ... absorbance of the solution at 562 nm was measured, and an extinction coefficient of 28 mm)1Æcm)1 was used to calculate the iron concentration Control experiments were carried out using an iron solution ... ampicillin to a D600nm of 0.6 Expression was induced for h with mm IPTG at 28 °C Cells were harvested by centrifugation at 3000 g for at °C, and the cellular pellet was stored at )20 °C The protein...
- 18
- 350
- 0
SUPERVISORY, INSTRUCTIONAL SKILLS AND PROFESSIONAL PERFORMANCE OF TEACHERS IN VOCATIONAL COLLEGES IN NGHE AN PROVINCE: BASIS FOR DEVELOPING AN ENHANCEMENT PROGRAM
... the motivation to develop vocational training under the direction of standardization, modernization, socialization, democratization and international integration Improving the quality and developing ... proper demonstration and execution of the lessons He can clarify classroom rules and procedures for students He can assist the teachers on presentation of a combination of instructional techniques ... Professional Performance of Teachers of Viet Han Vocational College 86 13 Table 13 Correlation of Supervisory and Instructional Skills of Teachers to their Professional Performance 88 vii LIST OF FIGURES...
- 117
- 375
- 0
Báo cáo sinh học: " Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein" pot
... formation of NP-induced intracellular inclusions, interaction of NP with VP35 and for the function of NP in RNA synthesis Materials and methods Cells and cDNA transfections HeLa, HUH7 and HUH-T7 cells ... NP and VP35 (binding is enhanced in the absence of C2) and is essential for the function of NP in transcription and replication of MARV RNA It is possible that the conformational flexibility of ... sonicated (3× 10s at -10°C), incubated for h at 4°C after the addition of 0.1% Triton ×-100 The suspension was cleared by centrifugation (8, 000 rpm, 4°C, 10 min) The supernatant was incubated...
- 8
- 393
- 0
Báo cáo hóa học: " Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein" pdf
... formation of NP-induced intracellular inclusions, interaction of NP with VP35 and for the function of NP in RNA synthesis Materials and methods Cells and cDNA transfections HeLa, HUH7 and HUH-T7 cells ... NP and VP35 (binding is enhanced in the absence of C2) and is essential for the function of NP in transcription and replication of MARV RNA It is possible that the conformational flexibility of ... sonicated (3× 10s at -10°C), incubated for h at 4°C after the addition of 0.1% Triton ×-100 The suspension was cleared by centrifugation (8, 000 rpm, 4°C, 10 min) The supernatant was incubated...
- 8
- 379
- 0
Báo cáo hóa học: " Research Article Design and Experimental Evaluation of a Vehicular Network Based on NEMO and MANET" pdf
... optimization problem statement,” RFC 488 8 (Informational), July 2007 C Ng, F Zhao, M Watari, and P Thubert, “Network mobility route optimization solution space analysis,” RFC 488 9 (Informational), ... these environments Second, traffic flows have to be allocated to appropriate paths depending on the application demands and network performances Since real-time applications are sensitive to handovers, ... integral communication platform for the ITS frame, which has been experimentally evaluated by means of a complete and open testbed In a first stage, an integrated evaluation environment for VANET enabled...
- 18
- 596
- 0
Báo cáo hóa học: " Research Article Detection and Parameter Estimation of Multicomponent LFM Signal Based on the Cubic Phase Function" pptx
... Wang and Y.-C Jiang [11] S Peleg and B Porat, “Estimation and classification of polynomial-phase signals,” IEEE Transactions on Information Theory, vol 37, no 2, pp 422–430, 1991 [12] S Peleg and ... no longer valid at low SNRs For the second component, we can see from Figure [1] T J Abatzoglou, “Fast maximum likelihood joint estimation of frequency and frequency rate,” IEEE Transactions on ... and Electronic Systems, vol 22, no 6, pp 7 08 715, 1 986 [2] R M Liang and K S Arun, “Parameter estimation for superimposed chirp signals,” in Proceedings of the IEEE International Conference on...
- 7
- 387
- 0
Báo cáo hóa học: " Research Article Bounded and Periodic Solutions of Semilinear Impulsive Periodic System on Banach Spaces" docx
... solution if and only if P has a fixed point Proof Suppose x · x · T0 , then x x T0 P x This implies that x is a x0 , x0 ∈ X, then for the P C-mild solution fixed point of P On the other hand, ... that there exists a constant M > such that x t, x − x t, y ≤ MMT0 x − y ≡ L x − y , ∀ t ∈ 0, T0 , 3.29 which implies that P x −P y x T0 , x − x T0 , y Hence, P is a continuous operator of x on ... Journal of Mathematical Analysis and Applications, vol 276, no 1, pp 98 1 08, 2002 13 X Xiang and N U Ahmed, “Existence of periodic solutions of semilinear evolution equations with time lags,” Nonlinear...
- 15
- 248
- 0
Screening of Bacillus strains as potential probiotics and subsequent confirmation of the in vivo effectiveness of Bacillus subtilis MA139 in pigs doc
... chloroform vapor for 30 The chloroform was then evaporated for 20 0.1 ml of a 24 h culture of E coli K 88 in Nutrient Broth (about 1 08 CFU/ml) was spread on the plate After 16– 18 h incubation at ... 37°C for 36 h and then heated at 80 °C by water bath for 15 to kill vegetative cells (Oscariz et al 1999; Foldes et al 2000) The surviving spores were collected by centrifugation (2500 · g for ... (2004) Field evaluation of the effect of a probiotic-containing Bacillus licheniformis and Bacillus subtilis spores on the health status, performance, and carcass quality of grower and finisher pigs...
- 8
- 420
- 0
Báo cáo lâm nghiệp: "Effects of a calcium deficiency on stomatal conductance and photosynthetic activity of Quercus robur seedlings grown on nutrient solution" pdf
... maintenance of CO concentrations in the sub2 stomatal spaces (c and a decrease of CO ) i at the carboxylation sites (c In contrast, ) c the activation state of Rubisco was reduced to 80 % We may exclude ... (1 984 ) Electronic microprobe study of variations in potassium concentration of stomatal complexes of two Commelinaceae Physiol Vég 22, 84 1 -84 9 von Caemmerer S, Farquhar GD (1 981 ) Some relationships ... the relationships existing between the known effects of Ca on the regulation 2+ of individual metabolic steps, and their consequences for photosynthesis and water relations at an integrated leaf...
- 11
- 370
- 0
Báo cáo y học: "Behavioral and antioxidant activity of a tosylbenz[g]indolamine derivative. A proposed better profile for a potential antipsychotic agent" pptx
... and its application to the prediction of transport phenomena Prediction of intestinal absorption J Pharm Sci 1999, 88 (8) :80 8 -81 4 SPARTAN SGI Version 5.1.3 OpenGL, Wavefunction, Inc., 184 01 Von ... surface area and its application to the prediction of transport phenomena Prediction of blood-brain barrier penetration J Pharm Sci 1999, 88 (8) :81 5 -82 1 Clark DE: Rapid calculation of polar molecular ... Low energy conformation and van der Waals surface of comLow energy conformation and van der Waals surface of compound TPBIA Page of (page number not for citation purposes) Annals of General Hospital...
- 7
- 400
- 0
Báo cáo y học: " Reliability and predictive validity of a hepatitisrelated symptom inventory in HIV-infected individuals referred for Hepatitis C treatment." docx
... tool for consideration of Cachay et al AIDS Research and Therapy 2011, 8: 29 http://www.aidsrestherapy.com/content /8/ 1/29 Page of Figure Correlation matrix displaying the correlations among symptom ... quantitative assessment of symptoms that were hypothesized to be associated with the decision to initiate or defer HCV treatment initiation, independent of standard of care clinical considerations ... lack of standardization for assessment of these symptoms among HCV/HIV patients being considered for HCV treatment initiation In April 20 08, a multidisciplinary hepatitis-HIV coinfection program...
- 9
- 483
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25