use of the in vitro model of stroke induced ae to evaluate the calcium hypothesis of epileptogenesis
Báo cáo y học: "In vitro model for the analysis of synovial fibroblast-mediated degradation of intact cartilage" pptx
... shown) Total RNA was then isolated using the RNeasy® Micro kit according to the supplier's instructions including a DNase digestion step Following removal of the SFB (in the case of co-culture), the ... potent inductors of aggrecanase activity in both cases, underlining the key role of these cytokines in proteoglycan depletion On the other hand, the clearly increased aggrecan activity in co-cultures ... (two pro-inflammatory cytokines centrally involved in the pathogenic process of RA) in order to simulate the influence of macrophage (leukocyte)derived pro-inflammatory cytokines on both chondrocytes...
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Báo cáo y học: " Cysteinyl-leukotrienes in the regulation of β2-adrenoceptor function: an in vitro model of asthma" ppsx
... in asthma may be the result of activation of the β2-AR by specific agonists (homologous desensitization) or activation of other receptors by the inflammatory mediators, which are present in the ... salbutamol -induced PKC inhibitorchallenged Effect of the pretreatment with the PKC inhibitor GF109203X on salbutamol -induced relaxation in challenged human bronchial rings Relaxant responses to salbutamol ... β2-AR desensitization in HASMC, thus ruling out the possibility of the involvement of this protein kinase Indeed, H89 tended to enhance the response to isoproterenol both in LTD4-challenged and...
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Báo cáo khoa học: Activator-binding domains of the SWI ⁄ SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo pot
... replace the recruiting function of the Swi1 and Snf5 activator-binding domains The in vivo validation of the Swi1 and Snf5 activator-binding domains is also of interest in relation to the twostep binding ... activation of these genes within the same time window The activator-binding domains in the Swi1 and Snf5 subunits of the SWI ⁄ SNF complex are essential for recruitment of the SWI ⁄ SNF complex to these ... with these activator-binding domains is thus critical for normal galactose induction of the tested genes Interestingly, the strains lacking either the FEBS Journal 276 (2009) 2557–2565 ª 2009 The...
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báo cáo hóa học: " A refined in vitro model to study inflammatory responses in organotypic membrane culture of postnatal rat hippocampal slices" potx
... demonstrate, the microglia are capable of complex behaviour and therefore it is of crucial importance to pay attention to the factors that contribute to the consistency of in vitro models used to mimic in ... Figure the Effect of3 dose of LPS on the inflammatory response in P7 hippocampus slices Effect of the dose of LPS on the inflammatory response in P7 hippocampus slices LPS was added to the medium ... express Toll-like receptor (TLR4) [27,28], which mediates the LPS induced intracellular NF-κB signaling pathway and evokes the release of cytokines To investigate whether the concentration of LPS...
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Báo cáo khoa học: "The response of dehydrated Douglas fir (Pseudotsuga menziesii) pollen to three in vitro viability assays and their relationship to actual fertility" doc
... models, the equations shown in figures 6-8 allow us to explain over 80% (see table V) of the variation within the data The remaining 20% of the variability is likely related to field pollination ... pollinator types (ie, paintbrushes and misting pollinators) It should be possible, then, to influence the proportion of applied male parents in Douglas fir using early pollination with lots of ... PEG The addition of 10% Brewbaker’s solution to the PEG concentrations completely eliminated the Class grains (fig 3B) Also, the addition of 10B to the concentrations of PEG further lowered the...
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Báo cáo y học: "CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model" pdf
... with integrins, whereas the cytoplasmic tail may be involved in regulation of the cytoskeleton through PLICs (proteins linking IAP to cytoskeleton) [32,33] Alternatively, inter- Available online ... modulate integrin affinity without intracellular signalling has been suggested [31] The extracellular IgV domain of CD47/IAP may interact with the integrin, inducing change to a high-affinity state ... following 0.083-Hz stimulation These cells, although expressing the extracellular IgV domain of CD47/IAP, not express the transmembrane domain or the intracytoplasmic tail of the CD47/IAP, indicating...
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Báo cáo y học: "Gender differences and inflammation: an in vitro model of blood cells stimulation in prepubescent children" pptx
... mount an inflammatory response than females with the beginning of infection This is exactly opposed to the actual in vivo situation The paradox must be explained by factors not included in the ex ... quantita- Page of tive and qualitative differences in the cytokine responses that could explain gender differences in the inflammatory profile Higher levels of cytokines in males could be explained by ... genes on the X chromosome that are involved in the inflammatory cascade [8-10] As the normal silencing process of one of the X chromosomes is incomplete in females [reviewed in [11]], some inflammation...
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Báo cáo y học: "Gender differences and inflammation: an in vitro model of blood cells stimulation in prepubescent children" pot
... mount an inflammatory response than females with the beginning of infection This is exactly opposed to the actual in vivo situation The paradox must be explained by factors not included in the ex ... quantita- Page of tive and qualitative differences in the cytokine responses that could explain gender differences in the inflammatory profile Higher levels of cytokines in males could be explained by ... genes on the X chromosome that are involved in the inflammatory cascade [8-10] As the normal silencing process of one of the X chromosomes is incomplete in females [reviewed in [11]], some inflammation...
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Báo cáo y học: "Propofol: neuroprotection in an in vitro model of traumatic brain injury" pot
... Due to the nature of the model it excludes mechanisms of injury that may influence brain damage in the in vivo situation such as the absence of any injury pathways related or due to brain swelling ... successful in both aetiologies of brain injury PI was used in this method as a staining agent to assess the degree of tissue injury PI labelling has been proven to correlate well with the extent of neuronal ... level of tissue injury was maintained in all slices prior to inclusion in the study groups This initial injury could be observed in all slices and is attributable to minimal cell death originating...
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Pathobiological studies of zoonotic blastocystis subtypes using in vitro model systems
... Strain -to- strain variation in Blastocystis induced drop in Caco-2 TER 4.3.2 Blastocystis ST-7 (B) induces an increase in Caco-2 epithelial permeability to FITC conjugated Dextran 4.3.3 Blastocystis ... illustrating prevention of Blastocystis -induced ZO-1 and actin rearrangement in Caco-2 monolayers by ROCK and HMG CoA reductase inhibition 151 Fig 5.6 Quantification of ZO-1 staining in Blatocystis-infected ... aim of this study was to investigate the pathogenic potential of Blastocystis, by studying the interactions of zoonotic Blastocystis isolates belonging to subtype-4 and subtype-7 with human intestinal...
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Tài liệu Báo cáo khoa học: Trophoblast-like human choriocarcinoma cells serve as a suitable in vitro model for selective cholesteryl ester uptake from high density lipoproteins pdf
... Results Binding of HDL to choriocarcinoma cells Specific binding of HDL3 to all three choriocarcinoma cell lines at °C was saturable at the highest lipoprotein concentrations (100 lg proteinÆmL)1) used ... pathway The fact that the C-terminal cytosolic domain of SR-BI which is a critical domain involved in selective CE-uptake [29,53] is lacking in SR-BII and the lack of SR-BII protein in all three ... was achieved by increasing the competitor concentration of unlabeled HDL3 to 500 lgÆmL)1 The addition of ox-LDL led to a pronounced inhibition in JAr and Jeg3 cells, findings in line with previous...
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Báo cáo sinh học: " In-vitro model systems to study Hepatitis C Virus" ppt
... etc.) to infect target cells HCVpp attach to the target cells, become endocytosed and fuse with the endocytic membranes to release the retroviral core containing the luciferase vector into the cytoplasm ... to produce long-term infection Infection of Daudi cells, a B-lymphoplastoid cell line, managed to produce long-term infection for up to year [20] but addition of the virus led to cellular cytopathic ... (HCVcc) in Huh- [29] Chimaeric constructs of JFH-1 with the structural region of the J6 genotype 2a clone improved the infectivity [30] Further refinements have led to the development of Huh7...
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Blastocystis investigations on host pathogen interactions using in vitro model systems
... activity of B hominis B and effect of inhibitors 52 Fig 2.3 Histogram showing the effect of parasite concentration 53 on the rate of azocasein hydrolysis by B ratti WR1 Fig 2.4 Histogram showing the ... Percentage of secretory IgA intact heavy chain remaining 75 after incubation with the lysates and conditioned medium of Blastocystis Table 3.2 Degrees of inhibition by different proteinase inhibitors ... studying the interactions of Blastocystis with intestinal epithelial cell lines This study reports that B ratti WR1 induces apoptosis in IEC-6 cells in a contact-independent manner Furthermore,...
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Báo cáo Y học: A Ca2+/CaM-dependent kinase from pea is stress regulated and in vitro phosphorylates a protein that binds to AtCaM5 promoter ppt
... found to be an in vitro substrate for the PsCCaMK, the effect of phosphorylation on its DNAbinding property was tested The protein was in vitro phosphorylated using cold ATP and used for DNA binding ... phytohormones) affect the level of same kinase and in turn different kinases are modulated by the same signal, depending on the specificity of calcium signal and position of the kinase in the ... similar to those of ZmCCaMK and p40 is one of its in vitro substrates To check the interaction of these proteins with DNA, we selected the promoter of the Arabidopsis CaM5 gene (AtCaM5) The reason to...
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Model Policy Guidelines for the Appropriate Use of Social Media and Social Networking in Medical Practice ppt
... authority to discipline physicians for unprofessional behavior relating to the inappropriate use of social networking media, such as: Inappropriate communication with patients online Use of the Internet ... on the other end of the communication For that reason, the standards of medical care not change by virtue of the medium in which physicians and their patients choose to interact The following ... applied to all aspects of a physician's practice, including online interactions through social media and social networking sites Referencing the FSMB House of Delegate’s Model Guidelines for the...
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Báo cáo y học: "Use of a highly sensitive strand-specific quantitative PCR to identify abortive replication in the mouse model of respiratory syncytial virus disease" pptx
... cycling (Table 1) The use of this tag is designed to reduce detection of cDNAs synthesised due to RNA self-priming in the reverse RSV infection in vitro To investigate whether RSV RNA synthesis ... observed in cultures infected with an MOI of 1, rising from day (105 copies) to day (106 copies) and was maintained until the end of the study at day 10 Similar to cells infected with the MOI of × ... polymerase chain reaction (QPCR) method to monitor the kinetics of RSV RNA replication in the mouse lung BALB/c mice were infected with RSV A2 and viral RNA in mouse lungs were monitored over an...
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Báo cáo khoa học: "Application of a zona pellucida binding assay (ZBA) in the domestic cat benefits from the use of in vitro matured oocytes" pptx
... in viability between fresh and FT spermatozoa may explain the differences in ZP binding registered However, the lack of binding to FT ZP is mainly due to the ZP and not to the spermatozoa, since ... and FT spermatozoa bound to the ZP of in vitro matured oocytes (Table 1, Figure 1) The percentage of binding of electroejaculated spermatozoa was higher in in vitro matured ZP than in the immature, ... suitable for use in feline ZBA, in contrast to the situation in other species Further studies are needed to explore the causes of our results Competing interests The author(s) declare that they have...
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The role of caspase 1 in a murine model of influenza pneumonitis, and studies on cell death inhibitors in vitro
... caspase-1 processes the IL-1β precursor (step 7) in the cytosol results in the generation of the mature IL-1β An influx of calcium into the cell (step 8) with an increase in intracellular calcium levels ... C-terminal CARD (ASC) is required together with CARDINAL for oligomerization The assembled components of the inflammasome initiate the processing of procaspase-1, resulting in the formation of the ... understanding of the mechanisms that undermine pathogenicity and the host-virus interactions that influence the outcome of the illness are critical for the control of influenza 1.2 Influenza viruses The...
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Báo cáo y học: "Use of chinese and western over-the-counter medications in Hong Kong"
... western medicine community pharmacists might be the only point of contact when they had minor aliments Therefore, there is a need in promoting and extending the roles of the western medicine community ... access to patients’ medical information are essential to integrate pharmacists in the primary care system [20] The role of medical professionals is a dominant factor in defining, controlling and ... when the integration with Chinese medicine is taken into account Previous research has already indicated the needs for western pharmacists to study Chinese medicine [24] A total of 26.2% of the...
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596 THE USE OF RELATIONSHIP MARKETING TECHNIQUES IN HIGHER EDUCATION A CASE STUDY
... 1.3 Kinh nghiệm chiến lược Marketing du lịch nước 12 1.3.1 Chương trình chiến lược Marketing du lịch đại 12 1.3.2 Kinh nghiệm thực chiến lược Marketing du lịch nước 13 1.3.2.1 Marketing ... xãy tương lai 1.3.2 Kinh nghiệm thực chiến lược Marketing du lịch nước: Tư tưởng Marketing giới phát triển theo ba xu hướng chính: Xu hướng Mỹ: Chú trọng phát triển Marketing theo hướng nghiên cứu ... TRƯỜNG ĐẠI HỌC KINH TẾ THÀNH PHỐ HỒ CHI MINH LÊ MẠNH HÀ Cao học kinh tế K.14 - Dalat ĐỊNH HƯỚNG CHIẾN LƯỢC MARKETING DU LỊCH DALAT - LÂM ĐỒNG ĐẾN NĂM 2020 Chuyên ngành : Quản trị kinh doanh Mã số...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25