... Graduate Management Admission Test, which isa standardized exam given at various locations in the United States and Canada and around the world Throughout North America and in many international ... as wideofa margin as any candidate in the state’s history (A) she was reelected with as wideofa margin as any candidate in the state’s history (B) she had been reelected with as wideofa ... a margin as any candidate in the state’s history (C) having been reelected with as widea margin as any candidate in the state’s history (D) she was reelected with as widea margin as any candidate...
... and 5-CTGAGGGAAGCAAG AATGGA-3, OXI1 (At3g25250): 5-GACGAGATTATC AGATTTTACGC-3 and 5-AACTGGTGAAGCGGAAG AGAC-3, PTI1-4 (At2g47060): 5-CCCCAAAGAAAATG AGTTGCT-3 and 5-GCATCATTTCCTGGAGGAAAG-3 Acknowledgement ... (Duchefa, Haarlem, The Netherlands) Tissues were cleared in ethanol and visualized with a stereomicroscope (Leica MZ16FA) RNA isolation and real-time quantitative RT-PCR analysis RNA was isolated ... buffer and [c-32P]-ATP MBP was used as an artificial substrate to assess the kinase activity and GST alone was used as a negative control The top panel shows the kinase assay visualized by autoradiography...
... antisense) for mouse Slc1 2a2 mRNA were designed as follows; Slc1 2a2 antisense, 5¢-ATCTTCACAAGAAAAAT CACCTGGTACCAAGGATGT; Slc1 2a2 sense, 5¢-ACAT CCTTGGTACCAGGTGATTTTTCTTGTGAAGAT All experimental protocols ... FEBS Z-i Ando et al Six1– ⁄ – ⁄ Six4– ⁄ – mice revealed specific anomalies in earlier stages of otic and nasal development and in the formation of branchial arch and some cranial ganglia, which ... GSE2043 (a complete list of these genes appears in Table S1 anda partial list is shown in Table 1) Because only a single microarray was used for each condition, genes with a relatively low level of...
... Raleigh JA & van der Kogel AJ (2000) Spatial relationship between hypoxia and the (perfused) vascular network in a human glioma xenograft: a quantitative multi-parameter analysis Int J Radiat ... demonstration of complete HIF- 1a degradation after each cycle of 30 of reoxygenation, showing that HIF- 1a had not accumulated in the course of intermittent hypoxia cycles, and therefore that it is ... enzymes that lead to extracellular matrix degradation (matrix metalloproteases) [75–78] In addition, NF-jB activation was reported as an early event in malignant transformation in vitro [79], and...
... molecules, LAPP (an approximately 13 kDa leech antiplatelet protein isolated from Haementeria of cinalis) and calin and saratin (approximately 65 kDa and 12 kDa proteins, respectively, both isolated ... Gruber for stimulating discussions, and Dr Barry Coller for the generous gifts of 6F1 and 6D1 Tara C White and Michelle A Berny are ARCS scholars, and David K Robinson is the recipient ofa Johnson ... dose–response and maximal aggregation of platelets did not differ in the presence of saratin (data not shown) However, onset of aggregation was significantly delayed in the presence of saratin (Fig 1A) , and...
... stratification Conclusions Identification of cytogenetic abnormalities using karyotyping for prognosis and treatment of hematological malignancies has been a standard diagnostic tool formany years ... were normalized to a value of 150 using the mas5 algorithm in the Affymetrix Microarray Analysis Suite 5.0 For subsequent analysis, the average probe intensity was used for triplicates Values of ... cycle and response studies CM participated in the design of the study and performed the statistical analysis YD, MAH, CM conceived of the study, and participated in its design and coordination and...
... been evaluated in laboratory studies of SARS-CoV: notable among these approaches are those using siRNA [7], passive antibody transfer [8], DNA vaccination [9], vaccinia or parainfluenza virus expressing ... FACS analysis BE performed data acquisition from the immunofluorescence experiments PR and TK provided critical reagents and revised the manuscript critically NS and SN along with MV and EB participated ... EB participated in the planning of the experiments, review and interpretation of data and critical review of the manuscript All authors read and approved the content of the manuscript Acknowledgements...
... stratification Conclusions Identification of cytogenetic abnormalities using karyotyping for prognosis and treatment of hematological malignancies has been a standard diagnostic tool formany years ... were normalized to a value of 150 using the mas5 algorithm in the Affymetrix Microarray Analysis Suite 5.0 For subsequent analysis, the average probe intensity was used for triplicates Values of ... cycle and response studies CM participated in the design of the study and performed the statistical analysis YD, MAH, CM conceived of the study, and participated in its design and coordination and...
... been evaluated in laboratory studies of SARS-CoV: notable among these approaches are those using siRNA [7], passive antibody transfer [8], DNA vaccination [9], vaccinia or parainfluenza virus expressing ... FACS analysis BE performed data acquisition from the immunofluorescence experiments PR and TK provided critical reagents and revised the manuscript critically NS and SN along with MV and EB participated ... EB participated in the planning of the experiments, review and interpretation of data and critical review of the manuscript All authors read and approved the content of the manuscript Acknowledgements...
... preparation SL carried out NK cytotoxicity assays and manuscript preparation EHG carried out statistical analysis and manuscript preparation TBG carried out patient referral, clinical data analysis ... analysis and manuscript preparation MHP carried out patient referral, clinical data analysis and manuscript preparation AF carried out study design, NK studies oversight and manuscript preparation ... preparation AAG carried out study design, project oversight, patient referral, data analysis and manuscript preparation All authors read and approved the final manuscript Acknowledgements This work was...
... functional and positional characteristics suggestive ofa role in the pathogenesis in SLE, andthat the potential of the cell death mechanism aponecrosis to contribute to disease warrants study ... receptor is proinflammatory and induces a form of cell death known as aponecrosis, which exhibits several characteristics of apoptosis We therefore suggest that the P2X7 receptor and gene have the ... L, Papucci L, Tani A, Schiavone N, Tempestini A, Orlandini GE, Capaccioli S, Orlandini SZ: Aponecrosis: morphological and biochemical exploration ofa syncretic process of cell death sharing apoptosis...
... the statistical analysis of the data and drafted the manuscript MTBR and PABR assisted directly the qRT-PCR assays and the caspase 3-like activity experiment.GLR assisted the Agro-infiltration ... NAC (NAM, ATAF1, ATAF2 and CUC2) domaincontaining proteins NAC proteins are plant specific transcriptional factors that are involved in a variety of developmental events as well as in biotic and ... Narusaka M, Ishida J, Nanjo T, Fujita M, Oono Y, Kamiya A, Nakajima M, Enju A, Sakurai T, Satou M, Akiyama K, Taji T, YamaguchiShinozaki K, Carninci P, Kawai J, Hayashizaki Y, Shinozaki K: Monitoring...
... dose and pattern of administration were in accordance with current standards Failure of organs was evaluated using the Sequential Organ Failure Assessment (SOFA) scale on admission and during ... continuous variables are expressed as median (25th and 75th percentiles) The association between risk factors and death was first examined by means of bivariate analysis This was accomplished using ... homozygous, and three bands at 139, 101 and 38 bp indicated a heterozygote at the -1082 locus Statistical analysis The main outcome measure was in-hospital mortality from any cause, but we also assessed...
... biophysical studies and neutralization assays, and drafted the manuscript DT participated to the neutralization and binding assays AA participated in the design of the study and writing of the manuscript ... BL and FC performed the neutralization assays involving ENF-resistant viruses MB conceived of the study, and participated in its design and coordination and writing of the manuscript All authors ... concentration of 10 μM and in a pH 7.2 buffer, displayed a positive peak after 195 nm and two negative maxima at 208 nm-222 nm, characteristic of α-helices (Figure 2) Quantification of the α-helical...
... Ras Rho ARF ClassI:ARF1, ARF2, ARF3 ClassII: ARF4, ARF5 Rab ARF Arl Arl1, Arl2, Arl3, Arl4, Arl5, Arl6, Arl7, ARFRP1, ARD1 ClassIII: ARF6 Fig Classification of mammalian ARF famlily small GTPases ... Arl3p, are homologues of mammalian Arl1, Arl2 and ARFRP1 respectively (Takai et al., 2001) 10 Table A comparison of the identities and divergences of the mammalian ARF and Arl subfamily of small ... for most members at the moment 12 13 human ARF1 human ARF mouse ARF2 mouse ARF2 human ARF3 human ARF3 human ARF4 human ARF4 human ARF5 human ARF5 human ARF6 human ARF6 rat Arl1 rat Arl1 human...
... Minneapolis, USA) This assay measures biologically active VEGF121 and VEGF165 Statistical analysis Differences between patients and healthy controls were evaluated using a non-parametric Kruskal-Wallis ... Critical Care Vol 12 No Kümpers et al package (SPSS Inc., Chicago, IL, USA) and the GraphPad Prism software (GraphPad Prism Software Inc San Diego, California, USA) Figure Results Decreased Ang-1 and ... coefficient and linear regression analysis was performed after logarithmic transformation of Ang-2 values (logAng-2) The primary outcome studied was 30-day survival and was calculated from the day of...
... presented as the mean ± standard deviation for variables with a normal distribution, and as the median and interquartile range for variables with skewed distributions Parametric or nonparametric ... were evaluated by the chi-square test for categorical variables Comparison of group differences for continuous variables was carried out by one-way analysis of variance or the Kruskal-Wallis test ... of data NM helped to draft the manuscript, and participated in the acquisi- Page of 10 (page number not for citation purposes) tion of data AZ participated in the coordination of the study AAZ...
... (2004) Mammalian GLD–2 homologs are poly (A) polymerases Proc Natl Acad Sci USA 101, 4407–4412 Nakanishi T, Kubota H, Ishibashi N, Kumagai S, Watanabe H, Yamashita M, Kashiwabara S, Miyado K & Baba ... active form of Xp54 RNA helicase in translational repression is an RNA-mediated oligomer Nucleic Acids Res 32, 1325– 1334 Nakahata S, Kotani T, Mita K, Kawasaki T, Katsu Y, Nagahama Y & Yamashita ... Arlot-Bonnemains for the Maskinp17 and AuroraA two-hybrid constructs We thank M Simonelig for critical reading of the manuscript, and J M Donnay and G Herrada for technical assistance This work was supported...
... primer pair (5¢-ATGGAGGCCGGAGATTTCAAAG-3¢, +1 to +22 bp; and 5¢-ACGGGCTTTAAGTATTTCATCAGGC-3¢, +1405 to +1428 bp) and actin primer pair (5¢-TTCGAGCAG GAGATGGCCACC-3¢ and 5¢-GAGATCCACATCTGYTG GAAGGT-3¢) ... ReverTra Ace (TOYOBO) The cDNA was amplified with TH-specific Regulation of melanin synthesis in insect cuticle primer pair (5¢-CAGCTGCCCAGAAGAACCGCGAGA TG-3¢, +11 to +36; and 5¢-GAACTCCACGGTGAACC AGT-3¢, ... separata TH and THs reported for other animals, including humans, are also > 70%, i.e 79, 78, 72, 72, and 71% with those of Drosophila melanogaster, Apis melifera, Rattus norvegicus, Mus musculus,...