... presence of the sulfation inhibitor Purification of HCII from plasma andfrom recombinant CHO cells HCII from outdated citrated human plasma of a single blood donor was isolated essentially as ... cofactor II: N-glycans and sulfation (Eur J Biochem 269) 985 Fig MALDI-TOF mass spectra of the reduced and permethylated total N-glycans enzymatically liberated from human HCII isolated (A) from ... carboxamidomethylation and tryptic digestion of HCII from plasma andfrom recombinant CHO cells One to two nanomoles of the purified protein were reduced, carboxamidomethylated and digested with trypsin;...
... spiking and incubation, it appeared that bio-oxidation of organic carbon and consequent acidification effected release of Ni, Cu, and Cd from F5 (residual fraction), of Ni and Zn from F3 fraction, of ... case of Ni, Cu, and Cd, into F4 fraction in case of Pb, Cu, and Cd, into F2 fraction in case of Ni, Cd, Pb, and Cu and invariably increased the F1 fraction (water soluble and exchangeable form) of ... distribution ofdifferent chemical pools of heavy metals in sludge and metal-spiked incubated sludge is depicted in Fig Spiking and incubation appeared to increase the proportions of Cd and Ni in F1 and...
... sequence of the a-subunit of MCRs from six methanogenic archaea of five different orders andfrom two methanotrophic archaea of the ANME-1 cluster The numbering is that for the a-subunit of MCR I from ... aminopeptidase M and MALDI-TOF MS b Sequence taken from Selmer et al [9] c Sequence determined via MALDI-TOF MS ⁄ MS d Sequence of peptide obtained from two different MCRs isolated from the Black ... biosynthesis of the nickel cofactor F430 [16] and for the post-translational modifications [9] are not yet known [17,18] Also, the in vitro reconstitution of active enzyme from its subunits and cofactor...
... observed from the same terrane or fromdifferent terranes; from clockwise rotation of the paleomagnetic vectors on the continental part to the counterclockwise rotation of the paleomagnetic ... of the African, Eurasian, North America and Indian Plates, and true polar wander since 200 Ma, Journal of Geophysical Research B96 (1991) 4029. [2] L.S. Chan, Paleomagnetism of ... sedimentary rocks from both sides of Red River Fault zone in Vietnam and tectonic interpretation, Journal of Sciences of the Earth 22 (2000) 266. B. Richter and M. Fuller, ...
... correlation of viral genotype to clinical phenotypes, and may help to reveal how viral genotypes affect the development of HAND During the assembly and annotation of the HBSD, we encountered a number of ... coded differently by multiple studies (NA118_p5, NA420_p6 and NA21_UK1) and cases of separate patients that were coded identically by different studies (NA20 and NA234) Combining sequences from ... HBSD to expand in several ways New deep sequencing projects will increase the number of sequences and expand the diversity of patients, sampling a wider spectrum of stages of disease and HAART...
... collected from the cities of Patras and Alexandroupolis Clinical HAV strains were collected during a major HAV outbreak among patients with acute hepatitis, from the hospitals of the cities of Alexandroupolis, ... plant of Patras and in one sample (1/50, 2%) collected from the entry-point of the treatment plant of Alexandroupolis Sera from eight hospitalized patients with acute hepatitis were collected and ... collected from the biological treatment plants of two urban cities (Patras and Alexandroupolis) in northern and southern Greece Different patterns of HAV endemicity have been detected in the cities of...
... characterizing, for the first time, the structure-function relationships of a larger panel of Envs derived from brain and other tissues (n = 81) Production and characterization of a panel of primary Env ... characterized a new panel of Envs (n = 81) derived from autopsy brain and other tissues of subjects who died from AIDS Production of three-dimensional gp120 models and characterization of the CD4bs cavity ... Macs3LN R5 Yes All R5 Brain Brain UK1-BR UK7-BR R5 R5 Yes Yes All R5 All R5 The clinical and neuropathological details of the study subjects, and the derivation and characterization of the primary...
... vectors Three types of cells (HEK293T, Hela and NIH-3T3) were transduced in triplicate with a series of doses of the vector (ranging from 0.1 to 15 ng of p24, measured by ELISA), and luciferase ... (integrating) and kept in medium keeping them in an undifferentiated state or glially differentiated state (by addition of 10% FCS) Differentiation of the cells by FCS leads to an increase of the transgene ... Figures 2a and 2b for which an unpaired t test was used Results and discussion We studied the effects of the incorporation of the Ig MAR on transgene expression from three types of LV containing...
... et al (1993), Getzy et al (1997), Gay and Nam (1998), Minton and Schrand (1999), Haushalter (2000), Mello and Parsons (2000), Allayannis and Ofek (2001) and Haushalter et al (2002) Lý thuyết ... sách tài sản (Haushalter, 2000; Hoyt and Khang, 2000; Allayannis and Weston, 2001; Allayannis and Ofek, 2001), giá trị sổ sách tổng doanh số bán hàng (Allayannis and Weston, 2001) Dấu biến kỳ vọng ... trị sổ sách vốn cổ phần ((Hoyt and Khang, 2000; Trang 12 / 29 Determinants of corporate hedging decision: Evidence from Croatian and Slovenian companies Allayannis and Weston, 2001; Mian, 1996),...
... “Determinants of corporate hedging decision: Evidence from Croatian and Slovenian companies”, Research in Internation Bussiness and Finance, 2012 41 Ying Liu, “The Determinants of Corporate Hedging and ... Empirical Research of European Firms”, Tilburg University, 2014 Haushalter, G.D., Financing policy, basis risk, and corporate hedging: Evidence from oil and gas producers The Journal of Finance 55 ... policies, The Journal of Financial and Quantitative Analysis 19 (2), 127–140, 1984 ****Smith, C.W., Stulz, R.M, The determinants of firm hedging policies, Journal of Financial and Quantitative Analysis...
... constants (LCs), band structures, densities of state and energy gaps ofall binaries, i.e., BaO, BaS, BaSe, BaTe, and BaPo, were calculated based on both the GGA and LDA methods from first- principles ... other methods 1.1.2 Band Structure, Density of State, and Energy Gap Band structures and DOS are useful properties in understanding the electronic behaviors of materials, especially the semiconductors ... zero and the pressure at this point is called as the metallization pressure So far, a lot of papers have been published for theoretical studies of the phase transitions and metallization of alkaline...
... analysis of the capacity of N ∗Nakagami-m channels Specifically, we have studied the influence of the severity of fading and the number of hops on the PDF, CDF, LCR, and ADF of the channel capacity ... into the influence of the number of hops and the severity of fading on the instantaneous channel capacity, and hence they are very useful for the design and performance analysis of multihop communication ... first- and second- order statistics of the capacity of N ∗Nakagami-m channels for applications in cooperative networks Gulzaib Rafiq∗1 , Bjøn Olav Hogstad2 and Matthias P¨tzold1 a Faculty of Engineering...
... indicated substitutions at O-3 of residue A, O-6 of residue B, O-3 and O-4 of residue C, O-3 of residue D, O-4 and O-6 of residues E and F, O-6 of residue G, O-5 and O-4 of residue L, whereas I was ... H-3eq of Kdo L and H-6 of Kdo I, whereas Kdo L was substituted by heptose A as indicated by the NOE effect found between H-1 A and H-5 L, and, in addition, between H-5 A and H-3ax L Allof these ... protons of the R-pyruvate group and H-4 and H-6 of GlcN residue Spectra were recorded at pD 14, 55 °C adjacent H-6 of residue E Thus, all main spin systems were assigned in the NMR spectra, and all...
... England) Dulbecco’s modified Eagle’s medium was from BioWhittaker; OPTI-MEM and Lipofectamine were from Gibco BRL; fetal bovine serum was from BioWhittaker; Hepes was from Fluka; geneticin was from ... and dimethylformamide were from Fluka N,N¢-diisopropylcarbodiimide was from Aldrich Dimethylformamide (pure) and diethylether were from Scharlau (La Jota, Barcelona, Spain) Acetonitrile was from ... sensitivity of 10 mdeg; step resolution, 0.2 nm; band width, nm The CD spectrum of the solvent was subtracted from the CD spectra of the peptide solutions to eliminate the interference from cell,...
... standard conditions RESULTS AND DISCUSSION Comparison of PP2A catalytic subunits from S cerevisiae and other species Phospholipids were solubilized in chloroform and after evaporation of chloroform ... case of pPIC3.5K-PPH22 and SalI in the case of pPIC3.5K-DN-PPH22 and pPIC3.5K-Nterm (N-terminus of Pph22p) or not digested, and transformed by the spheroplast method into KM71 and GS115 strains of ... inhibitory effect of the N-terminus Characterization of purified Pph22p and DN-Pph22p Fig Purification of Pph22p and DN-Pph22p from overexpressing P pastoris cells Aliquots of Pph22p and DN-Pph22p...
... written by K Hofmann and M D Baron) or manually modified Secondary structure prediction was by the methods of Chou & Fasman [32] and Garnier et al [33] using GCG software Protein and gene nomenclature ... sequences of PSRP-4, PSRP-5c (the shortest form of PSRP-5 [2]), PSRP-6 and Thx (manually aligned) and the schematic diagram of a discernible consensus secondary structure, composed of an N-terminal ... 5¢-termini and 3¢-termini of PSRP-2 and PSRP-4 cDNAs (P2-3, P2-4, P4-3, and P4-4) were designed after sequencing the sets of PCR products The full cDNAs encoding PSRP-2 and PSRP-4 were amplified from...
... stucture and function of the rotor of the Na+ F1F0-ATP synthase from A woodii, the first containing V0- and F0-like c subunits Experimental procedures Growth of cells and isolation of membranes A woodii ... B, and resuspended in 60 mL of buffer B luminol), and solution B (10 mL of dimethylsulfoxide containing 11 mg of p-hydroxycoumaric acid)] Blot membranes were incubated in a mixture of mL of solution ... F1F0-ATP synthase from A woodii M Fritz and V Muller ¨ of proteoliposome solution from gel filtration and by adding mm ADP The synthesis reaction was started by the addition of mL of valinomycin...
... representative, milk from three different women was analyzed as1-Casein was purified and the reversed-phase traces of tryptic digests of the protein were compared and found to be identical in all cases, ... experience of employing MALDI-TOF mass spectrometric analysis for identification and localization of phosphorylation sites in proteins [16–18] MALDI-TOF mass spectrometric analysis of a phosphopeptide ... Edman sequencing of the peptide, which showed normal yields of PTH-serine in all relevant cycles Mass analysis of peak 35–1 showed a mass of 1942.78 Da, as well as two populations of ions at approximately...
... to determine the standard curve of the crossing points for the amplification of B-FABP and b-actin from tissue-specific cDNA samples Melting curve analysis of each standard and experimental sample ... extrapolation from the standard curve of concentration-dependent bacteriophage lambda DNA fluorescence and the copy number per lL was calculated Five dilutions of the B-FABP and b-actin product ranging from ... [27] Mapping of the initiation site of transcription for the zebrafish B-FABP gene Fig Structure of B-FABP genes from fishes and mammals (A) Comparison of the exon/intron organization of the zebrafish...