... in pulmonary epithelial cells is currently lacking As NF-jB is considered to be a candidate for therapeutic intervention in airway in ammation, we have investigated the role of NF-jB in the induction ... NF-jB binding site (bold text) upstream of a minimal thymidine kinase promoter driving a luciferase gene Confluent cellsin 24-well plates were changed to serum-free medium and treated as indicated ... effectiveness of this construct in repressing both NF-jB DNA binding and transcriptional activity inA549cells [19] Both TNFa and IL-1b produced a robust increase in reporter activity, measured...
... apoptotic cells was determined by staining cells with annexin V-FITC and propidium iodide (PI) The annexin V-FITC apoptosis detection kit was purchased from Calbiochem (Canada) After incubation, cells ... apoptotic cells from the time-dependent study *p < 0.05, **p < 0.01 Fig The effect of tetrandrine on the proliferation of A549cells The viability of A549cells was measured using the MTT assay The cells ... apoptotic cellsin the inhibitor study Data are presented as mean ± SE of independent experiments **p < 0.01 Fig The effect of tetrandrine (TET) on ERK activation inA549cells The cells were...
... procured locally Cell line and culture conditions The human lung cancer cell line A549 was obtained from the China Center for Type Culture Collection (Wuhan, China) and maintained in RPMI-1640 supplemented ... penicillin, and 100 μg/ ml streptomycin at 37°C in a humidified atmosphere of 5% CO2 MTT Assay Cell proliferation was measured using the MTT assayA549cells were plated at a density of × 10 4cells ... apoptosis by targeting the proteins of Bcl-2 family and the ratio of Bax/Bcl-2 played a critical role in determining whether cells will undergo apoptosis [31,32] In our study, by examining the effect...
... others, in experiments with other cell lines [13,14] In order to ensure that the experiments were in fact measuring apoptosis, we also assessed apoptosis by TUNEL staining The finding that following ... 0.05) increase in the number of apoptotic cells (Figures 2a and 2b) While treatment with Taxol also caused a significant (p < 0.05) increase in the number of "indeterminate" cells Interestingly, ... damage induced after tobacco smoke exposure TUNEL staining was used to examine DNA damage inA549cells 24 and 48 hours after treatment (Figures 3a and 3b) Since the number of detached cells varied...
... infection inA549cells which are not activated in response to RG modified H5N1 virus infection Influenza virus infection results in activation of various signaling events in the host cellsIn ... Genes involved in chemokine & cytokine mediated signaling in highly-pathogenic and RG modified H5N1 infected A549 cell lines (16 h post infection time point) Red arrow indicates expression in highly-pathogenic ... containing 10% fetal calf serum, 100units/ml penicillin, 100 units/ml streptomycin in tissue culture flasks (Corning, USA) at 37°C in a CO2 incubator Virus infection Monolayers of A549 cells...
... nucleoprotein (NP) and matrix protein (M1) in the infected cells was examined by using immunofluorescent staining and Western analysis, respectively Positive staining of the NP protein was found in both ... both H9N2-infected and H1N1-infected A549cells at 24 h post-infection (Fig 2A) Interestingly, strong M1 protein expression was observed incells infected with H9N2/G1 at h and 24 h post-infection ... protein and acid polymerase protein (PA) in virus-infected A549cells at h, h and 24 h post-infection by using quantitative RT-PCR assay [11] The coding regions of the respective viral genes in...
... replicated in flow-cytometry analyses of infected A549cells (Fig 6a and 6b) 40 Figure growth of L pneumophila inA549cells Intracellular Intracellular growth of L pneumophila inA549cellsA549cells ... mitomycin C mitomycin C mitomycin C/m-P pan-actin Figure detectedactivity in L blotting Caspase8by western pneumophila-infected A549cells Caspase activity in L pneumophila-infected A549cells ... nuclear DNA fragmentation in infected A549cells as a possible mechanism of L pneumophilainduced cytotoxicity by TUNEL staining A549cells infected with the virulent strain of L pneumophila, AA100jm,...
... Medicine 57 Reagents F12 culture medium (Hangzhou Jino Biology Co., Ltd China), fetal bovine serum (FBS), ampicillin, penicillin and streptomycin (Shanghai Bio-engineering Co., Ltd China), brain ... O2, 85% N2, 10% CO2) with continuous shaking E coli were maintained in the LB liquid medium (containing 100 µg/ml ampicillin) at 37°C for 12 h with continuous shaking Preparation of Hp and E.coli ... Ltd USA) were used in the present study Cells and Hp Culture AGS cells were grown in the F12 culture medium containing 10% FBS, 100 U/ml penicillin and 100 µg/ml streptomycin in a humidified atmosphere...
... almost no increase in the phosphorylated JNKs protein level in the cell line Twenty-four-hour exposure to hypoxia resulted in a Int J Med Sci 2007, 42 marked increase (1.8-fold of control) in the ... Morpho/AS was examined using a fluorescent microscope Fluorescence was mainly observed in cytosol areas in transfected cells FITC-labeled Bag-1 Morpho/AS was transfected into almost all JAR cells by EPEI-mediated ... and methods Cell line and cell cultures The human choricarcinoma cell line JAR was obtained from American Type Culture Collection (Manassas, VA, USA) Cells were maintained in RPMI 1640 medium...
... after its initial effect on protein kinases phosphorylation The most strikingly and novel finding obtained hereby was the strong inhibition of the GPCR signaling pathway induced mainly by increased ... proteins Oncogene 1996;12:1-9 18 Hoek JB, Pastorino JG Cellular signaling mechanisms in alcohol-induced liver damage Semin Liver Dis 2004;24:257-72 19 Aroor AR, Shukla SD MAP kinase signaling in ... of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 1976;72:248-54 24 Pelech S, Sutter C, Zhang H Kinetworks protein kinase multiblot analysis Methods...
... G Elevated c-Jun expression in acute myeloid leukemias inhibits C/EBPalpha DNA binding via leucine zipper domain interaction Oncogene 2003;22(30):4760-4 Staber PB, Linkesch W, Zauner D, Beham-Schmid ... exon 10 and adjacent intronic sequences we could for the first time identify a new single nucleotide polymorphism (SNP) in the intron sequence flanking the 3’ end of exon 10 in out of 35 (20%) ... approximately 101 bp and 444 bp in size in samples either containing the heterozygous C/T or homozygous T/T SNP while these fragments could not be detected in samples lacking the SNP and therefore...
... involved in claudin-1 expression and localization 99 Indeed, a study shows that increasing β-catenin increases the expression of claudin-1 in colorectal cancer cells, and that claudin-1 expression ... using bryostatin instead increased the expression of claudin-1 in the tight junction complex, and increased tight junction barrier integrity (9) In melanoma, we have shown that claudin-1 expression ... melanoma cells (4) To determine if the nuclear localization of claudin-1 was related to the increased invasive capacity of melanoma cells, we created a claudin-1 expression vector expressing claudin-1...
... (GO:0007166) The basigin protein was shown to be present in vascular endothelium in both in the non-neoplastic region of the brain and in tumor cells (http://amigo.geneontology.org/cgi-bin/amig o/gp-details.cgi?gp=UniProtKB:P35613) ... cannot determine the global players involved in such complex phenotypes Integration of the gene expression profiling with specific modulation of gene expression in relevant signalling pathway ... Software and are described in the following part according the definitions of the Illumina’s (ILL) data as well as of the Illumina’s annotations (table 2) http://www.medsci.org Int J Med Sci 2009, 22...
... variety of cell types, including hepatocytes, to long-chain saturated fatty acids (SFAs) led to increased expression of proinflammatory cytokines, inhibition of insulin signaling, induction of endoplasmic ... Pharmigen, San Diego, CA, USA) binding and PI staining were performed according to [44] Following incubation with 1.0 lgÆmL)1 annexin V and 4.0 lgÆmL)1 PI for 20 min, cells were analyzed by flow cytometry ... results that TAG synthesis was initially hindered following stearate administration and was completely interrupted at longer incubation periods Interestingly, the interruption of TAG synthesis...
... expressed GAPDH–citrine (chimeric protein with N-terminal GAPDH and C-terminal citrine) and citrine–GAPDH (chimeric protein with N-terminal citrine and C-terminal GAPDH) were localized in the cytoplasm ... on the length of linkers In the linker containing seven amino acids, a Pro-Pro sequence that is not contained in other linkers is incorporated This less flexible structure may inhibit fluorophores ... obtained when cell extracts containing GAPDH–cerulean (chimeric protein with N-terminal GAPDH and C-terminal cerulean) or cerulean–GAPDH (chimeric protein with N-terminal cerulean and C-terminal...
... as inactive proenzymes and latency is maintained by an interaction between a cystein residue in the prodomain and Zn2+ in the active site of the catalytic domain Two major types of endogenous inhibitors ... polymerization took place Cell viability assay To determine the viability of the cells, trypsinized cells were suspended in serum-containing medium In order to remove serum, cells were washed three times ... inhibitors in several cell lines [10] The protein itself has no known enzymatic activity, but binds to distinct intracellular target proteins and regulates specific functions involved in tumour...
... highly specific peptidebinding domain found in poly(A)-binding protein and a HECT ubiquitin ligase EMBO J 23, 272–281 Craig A (1998) Interaction of polyadenylate-binding protein with the elF4G homologue ... to interact with eIF4G at a stoichiometry of more than : It should be noted here that, in addition to the C-terminal domain, PABP1 has another eIF4G-binding domain within its N-terminal RNA-binding ... growth medium containing 20% fetal bovine serum was added Following a total of 12 h of incubation, the medium was replaced with fresh DMEM containing 10% fetal bovine serum Cells were usually...
... to in ammatory sites [14,15] and adhesion of polymorphonuclear cells and monocytes to CNS cells [16,17] Schwann cells (SCs) are glia cells found in the PNS In addition to their roles in myelination, ... al LPS increases ICAM-1 expression in Schwann cells of sciatic nerves A B C D Fig LPS induced the expression of ICAM-1 protein in cultured SCs (A) LPS induced ICAM-1 protein expression in a concentration-dependent ... responses in the PNS [31] These cells may function as antigen-presenting cells and activate T cellsin vitro in an antigen-specific and MHC-restricted manner [32], especially in the presence of cytokines...