... Szymczak, Alexander I Voitenko , Masaru Kato, Takekazu Ishida, Tomio Koyama, Masahiko Machida, Florian Loder, Arno P Kampf, Thilo Kopp, C .A. C Passos, M S Bolzan, M.T.D Orlando, H Belich Jr, J.L Passamai ... Structures of d-Wave and s-Wave Superconductors (d-Dot): Analysis Using Two-Component Ginzburg-Landau Equations Masaru Kato, Takekazu Ishida, Tomio Koyama and Masahiko Machida 319 Chapter 14 Flux-Periodicity ... Kobayashi, H., Balicas, L and Brooks, J S., Superconductivity in an Organic Conductor Stabilized bya High Magnetic Field, Advanced Materials 14, 243 (2002) [9] Kobayashi, H., Kobayashi, A. , Cassoux,...
... condensation ofa molecule of b-alanine witha molecule of pantoate in an ATP-dependent manner to form pantothenate [1,2] Pantothenate itself is an important cofactor that is essential for CoA biosynthesis, ... and Glu for M tuberculosis and A thaliana PS) As compared with the substratefree form, the imidazole rings of His34 in the A- chain ˚ ˚ and B-chain of nPS move byA and 5.5 A, whereas the backbone ... Gopalakrishnan B, Aparna V, Jeevan J, Ravi M & Desiraju GR (2005) A virtual screening approach for thymidine monophosphate kinase inhibitors as antitubercular agents based on docking and pharmacophore...
... interpretation of nuclear magnetic resonance spectra of proteins Q Rev Biophys 23, 1–38 Yamazaki T, Yoshida M, Kanaya S, Nakamura H & Nagayama K (1991) Assignments of backbone 1H, 13C, and 15N resonances ... exclude glutamine, glutamate, asparagine and aspartate from the labelling scheme because of excessive cross-labelling [17] In order to avoid the useof expensive [15N]amino acids, this particular in ... that adequate protein yields can be obtained from, at most, a couple of milligrams of each amino acid [18] A more sophisticated combinatorial labelling scheme has been proposed by Parker et al...
... Oliveira FW, Chavante SF, Santos EA, Dietrich CP & Nader HB (1994) Appearance and fate ofa beta- 13 14 15 16 17 18 19 20 21 22 23 galactanase, alpha, beta-galactosidases, heparan sulfate and chondroitin ... source and age, but within a single chain there is variability The chain cap of CS is a GalNAc or GlcA residue; a 4,6-disulfated GalNAc residue, rare in the repeat region of human articular cartilage ... repeat region of CS is a repeating disaccharide of glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc) [-4)GlcA(b1– 3)GalNAc(b1-]n, which may be O-sulfated on the C4 and ⁄ or C6 of GalNAc and...
... and an eluent containing mM ammonium acetate in 25% methanol Riboavin, FMN and FAD had retention volumes of 44 mL, 10 mL and mL, respectively 5Â-TCAGAATTCCATGGATATTTGGTA CGG-3Â 5Â-GGCCAACGCAAAGGGATCCTCGAT ... from the National Institute of General Medical Sciences (to C H W.) and by the Health Services and Research Administration of the Department Administration of the Department of Veteran Aairs (to ... p.p.m.) than that of TARFH2 This fact can be ascribed to an increased p-electron density at the N(10) atom in comparison to that of free avin, and, consequently, an increased sp3 hybridization of...
... Tanemura K, Murayama O, Akagi T, Murayama M, Sato S, Sun X, Tanaka N & Takashima A (2001) New insights on how metals disrupt amyloid beta-aggregation and their effects on amyloid-beta cytotoxicity ... aggregated and ⁄ or precipitated Assuming that zinc and To monitor the structural changes of Ab induced by metal ion interactions, CD spectra were recorded for Ab with increasing amounts of added ... exhibit a total loss of H6 and H14 resonances, and a weak H13 resonance remains at the same chemical shift The tyrosine aromatic crosspeaks are unaffected upon zinc addition B A H13 H6 FEBS Journal...
... in the phase I Intracellular ATP and ADP concentrations in phase I were estimated based on 31P NMR data The data shown are the mean ± SD Fig Changes in cellular ATP, ADP and ATP/ADP of the XMP ... intracellular metabolites, NADP was used as a concentration standard in the NMR Partially relaxed 8,9 MDP was used to estimate metabolite concentrations ND, not determined Values are the mean ± SD Average ... phase (A) Cellular ATP (s) and ADP (d) concentrations are shown (B) Cellular NADP (j) is shown To quantify the intracellular metabolites, MDP was used as a concentration standard in the NMR All...
... production ofa Histagged SaDHNA The primers for the PCR were 5¢-GG AATTCCATATGCAAGACACAATCTTTCTTAAAG-3¢ (forward primer witha Nde I site) and 5¢-CGGGATCCT CATTTATTCTCCCTCACTATTTC-3¢ (reverse primer with ... The Authors Journal compilation ª 2007 FEBS 2249 Mechanism and kinetics of dihydroneopterin aldolase Y Wang et al 5¢-GGAATTCCATATGGATATTGTATTTATAGAGCA AC-3¢ (forward primer witha Nde I site) and ... Y Wang et al Fig Global analysis of the quench-flow data of the SaDHNA-catalyzed reaction Data 1, 2, 3, 7, 8, and 11 were obtained with DHNP as the substrate Because the commercial DHNP contained...
... demonstrate the structural importance of the nature of the amino acid at position 81 of the encephalitogenic sequence 74–85 of guinea MBP: replacement of Asp81 with an alanine seems to break a chain ... between charged groups in water In the agonist, ˚ the side chain of Arg78 is almost always less thanA from a negatively charged group, mainly Asp81 and Glu82, but also the COO– terminal of Val85 ... (Ala81–Val85) appears to be much more flexible, with Ala81 far distant from the side chain of Arg78 The side chain of Arg78 is less well defined, as in the case of aqueous solution, because of the absence of...
... chemical exchange for Asp18 or Trp33sc with increasing amounts of some sugars Peak movements of main chain amide and amide proton of Asp18, and side chain amide and amide proton (marked by ‘sc’) of ... spaced 50 ms Gaussian-shaped pulses for saturation with ms intervals and a total saturation time of s On-resonance irradiation of the protein was conducted at a chemical shift of –0.4 p.p.m and ... binding of each of sugar; lactose, melibiose, galactose (all from Wako Chemicals, Tokyo, Japan), a- Me-Gal and b-Me-Gal (both from Seikagaku Co., Tokyo, Japan), to EW29Ch at 25 °C (pH 6.1) was measured...
... Thanks to Associate Professor Low Boon Chuan and Zhong Dandan for their help as collaborators Thanks to Assistant Professor Liang Zhao-Xun, Dr Nikolay Korolev and Abdollah Allahverdi in Nanyang ... equivalently, the decay of transverse single-quantum coherences (Cavanagh 2007) Analysis of the relaxation ofa system provides a great deal of information about the geometry and dynamics of the ... both mammalian cells and yeast (Zhang et al 2000) An intact PNT domain (moniker of SAM domain) of Ets2 specifically recognized a Cdc2-related kinase, Cdk10 (Kasten and Giordano 2001) In addition,...
... patients in the comparator group with P aeruginosa at baseline than for those without P aeruginosa For example, median duration of mechanical ventilation was 10 days for all patients with VAP ... (discharge date or death date) - randomization date + If discharge date was not available, patients were censored at late follow up In addition, all-cause overall mortality and, in patients with P aeruginosa ... proportional hazards assumption was validated for each model Statistical Analysis Software version 9.1.3 (SAS Institute, Inc., Cary, NC, USA) was used for statistical analyses P values less than 0.05...
... hippocampal and parietal cortical regions [47]; a separate analysis of auditory-evoked potentials via brain electric source analysis and multiple-dipole modelling indicates putative generators ... is also notable that the detection ofa frontal N2 is associated only with instances ofa NOGO response Thus, the P300 appears to be inhibited by the appearance of the N2b in tasks of motor activity ... similar oddball paradigm using short inter-stimulus intervals, however, MMN latency and amplitude varied little as a result of increasing age, suggesting the invariance of automatic stimulus analysis...
... indicates that estimation accuracy has improved, and focus on risk management has increased Ericsson Software Technology The company claims that the initiative was "more valuable" thana database ... done by collecting information that was already documented in the company, and to make it available and searchable, a kind ofa bottom up way to start a knowledge management program The article ... for useofa tool and experience with it Management Packages reference information for project managers Data Packages data relevant for a software project or its activities Can be project databases...
... Chemistry and Toxicology of Mushrooms, and Characters of Mushrooms, to which their names are appended, and also to Dr Chas Peck, of Albany, N Y., and Dr G Bresadola, of Austria-Hungary, to whom some of ... on our part, as well as some experience in judging of the value of such characters; the same habit of observation and discrimination we apply to everyday affairs and to all departments of knowledge ... enlarge and elongate and make their way toward the surface of the ground They are at first very minute and grow from the size ofa pinhead to that ofa pea, and larger Now they begin to elongate...
... I, Pimkhaokham A, Nakagawa T, Kamata N, Kozaki KI, Amagasa T & Inazawa J (2007) PRTFDC1, a possible tumor-suppressor gene, is frequently silenced in oral squamous-cell carcinomas by aberrant promoter ... Canyuk B, Focia PJ & Eakin AE (2001) The role for an invariant aspartic acid in hypoxanthine phosphoribosyltransferases is examined using saturation mutagenesis, functional analysis, and X-ray ... regulatory ligands for PRTFDC1 The catalytic efficiency and substrate specificity of PRTFDC1 was further characterized using a radiochemical assay with tritium-labeled bases as substrates, whereas...
... Authors Journal compilation ª 2009 FEBS 509 Pyruvate synthesis by pyruvate oxidoreductase T Ikeda et al 34 Ozawa Y, Nakamura T, Kamata N, Yasujima D, Urushiyama A, Yamakura F, Ohmori D & Imai T (2005) ... enzyme activity of the recombinant LDH was assayed at 70 °C by monitoring the lactate-dependent NADH oxidation as the decrease in A3 40 The standard assay mixture contained mm lactate, 0.2 mm NADH and ... was incubated at 70 °C under an argon atmosphere The reaction was started by adding the NADH, acetyl-CoA and enzyme solutions to the mixture, and the decrease in A3 40 as a result of NADH oxidation...
... homes.129) Nano -particles and Nano-materials The health effects of nano -particles and nano-materials have been reviewed.117–121) Nanomaterials are divided into two types: environmental nano -particles ... (in Japanese) IPCS (1989) Environmental Health Criteria 89 Formaldehyde, World Health Organization, Geneva Takeda, M., Saijo, Y., Yuasa, M., Kanazawa, A. , Araki, A and Kishi, R (2009) Relationship ... Indoor Air Quality Indoor Environ., 1, 27–34 (in Japanese) 126) Kishi, R., Saijo, Y., Kanazawa, A. , Tanaka, M., Yoshimura, T., Chikara, H., Takigawa, T., Morimoto, K., Nakayama, K and Shibata, E (2009)...
... Finland) and Vent and Taq Endonuclease I from V salmonicida and V cholerae Table List of PCR primers Restriction sites are underlined No Sequence GCTTTTAAAGTTGACTTCAAAG CTTTGAAGTCAACTTTAAAAGC CTACCATGGCACCTCCTTCTTCTTTCTCAA ... CTTTGAAGTCAACTTTAAAAGC CTACCATGGCACCTCCTTCTTCTTTCTCAA GCTGTCGACTTATTTAGTGCATGCTTTATAAACAA CTACCATGGCCCCCATCTCTTTTAGTCAT GCTGTCGACTCAGTTCGGGCATTGCTCAC polymerase from Promega were used in the PCRs Restriction ... water Knowledge on cold adaptation is in many cases based on marine secreted enzymes Detailed data on salt adaptation of marine cold-adapted secreted enzymes is lacking and may be a source of...