trained by human knowledge on multiple clinical cases
Fibroblast-like synovial cells from normal and inflamed knee joints differently affect the expression of pain-related receptors in sensory neurones: a co-culture study ppsx
... different culture conditions The open bars in the graphs show the proportions of conditions neurones (%) in the indicated size ranges; the black insets show the proportions of neurones that express ... therefore, it is difficult to make firm conclusions about whether FLS cells exert trophic influences on neurones under non-inflammatory conditions However, comparison of the effects of FLS cells from ... that the expression of NK1 receptor-like IR in DRG neurones is upregulated by long-term addition of either PGE2 [43] or IL-6 [46] to the culture medium, we measured the concentration of PGE2 and...
- 12
- 184
- 0
Báo cáo hóa học: " Investigating the Influence of PFC Transection and Nicotine on Dynamics of AMPA and NMDA Receptors of VTA Dopaminergic Neurons" doc
... injection within one hour [8] Additionally, long term potentiation (LTP) was rapidly induced by afferent stimulation and lasted more than one hour in VTA slice [9] Moreover, a positive correlation ... provides more information than traditional measures -9- based on only peak ratios Specifically, as mentioned in the method section, we estimated areas under AMPA and NMDA curves on consecutive 50ms ... potentiation in dopamine neurons Nature 2001, 411: 583-587 Saal D, Dong Y, Bonci A, Malenka RC: Drugs of abuse and stress trigger a common synaptic adaptation in dopamine neurons Neuron 2003, 37: 577- 582...
- 28
- 431
- 0
Báo cáo hóa học: " Expression of HIV receptors, alternate receptors and co-receptors on tonsillar epithelium: implications for HIV binding and primary oral infection" doc
... infection in the absence of CD4 recognition by gp120 projecting from the envelope of HIV virions [34-36] Additional interactions between virions and mucosal surfaces may be supported by host ... HIV infection in vitro [31,50] A detailed evaluation by fluorescence activated cell sorting (FACS) of co-receptor expression on tonsil cell suspensions provided valuable information relating ... HIV infection Based on a comprehensive interpretation of the expression patterns revealed in this study, we conclude that multiple distinct interactions may be supporting HIV virion binding to...
- 13
- 308
- 0
Báo cáo khoa hoc:" Expression of estrogen and progesterone receptors in vestibular schwannomas and their clinical significance" docx
... progesterone receptors could be detected in any of our cases by means of well known immunohistochemical method using well documented monoclonal antibodies (Figure 2) In the control Figure demonstrable ... Labit-Bouvier et al 2000 69 none 10 10 Cafer et al 2008 59 none 100 11 Dalgorf et al 2008 none none 12 Jaiswal et al 2009 100 none none Page of (page number not for citation purposes) Journal of Negative ... retrospectively determine the expression of estrogen and progesterone receptors by means of immunohistochemical methods using monoclonal antibodies in 100 cases of vestibular schwannomas and also...
- 5
- 252
- 0
Báo cáo khoa học:" Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection" pptx
... particles in cotton rat cells Results Isolation of cotton rat cells expressing human CD4 and HIV-1 co-receptors To demonstrate that cotton rat cells lack the strong transcriptional blockages for ... citation purposes) Virology Journal 2009, 6:57 http://www.virologyj.com/content/6/1/57 Figure Isolation1and cloning of cotton rat cells expressing human co-receptors for HIV-1 Isolation and cloning ... production by cotton rat transfectant cells after HIV-1 infection Next, we infected the engineered cotton rat CD4 clones to define the time course of p24gag production (Fig 2) When the CCRT K4 clone...
- 9
- 329
- 0
Báo cáo khoa học: " Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection" pdf
... particles in cotton rat cells Results Isolation of cotton rat cells expressing human CD4 and HIV-1 co-receptors To demonstrate that cotton rat cells lack the strong transcriptional blockages for ... citation purposes) Virology Journal 2009, 6:57 http://www.virologyj.com/content/6/1/57 Figure Isolation1and cloning of cotton rat cells expressing human co-receptors for HIV-1 Isolation and cloning ... production by cotton rat transfectant cells after HIV-1 infection Next, we infected the engineered cotton rat CD4 clones to define the time course of p24gag production (Fig 2) When the CCRT K4 clone...
- 9
- 251
- 0
Báo cáo khoa học: "The early responses of VEGF and its receptors during acute lung injury: implication of VEGF in alveolar epithelial cell survival" ppt
... among the groups (Figure 2b) The expression and distribution of VEGF in the lung tissues were examined with IHC VEGF expression in control and sham-operated animals was characterized by strong ... had no effects on cell viability and protein levels of VEGF and VEGFR1 in comparison with non-treated control Discussion The present study is a comprehensive report on the early responses of VEGF ... correlation studies are small; thus, these results should be interpreted with caution We noted a significant correlation of the VEGF concentrations with the total protein concentrations and with...
- 13
- 290
- 0
Serotonin and serotonin receptors in neural stem and progenitor cell proliferation
... neurons This may suggest a dual mechanism by glutamatergic input, one to inhibit NSPC proliferation and the other to maintain cell survival during neuronal maturation Upon migration to the region ... the rate of proliferation Multipotent Differentiation after Vitrification 2.4 Discussion 81 SEROTONERGIC FIBRES AND 5-HT IMPLICATIONS FOR NSPC PROLIFERATION 3.1 Introduction 92 3.2 3.2.1 3.2.2 ... the likelihood of direct serotonergic control of NSPC proliferation The notion of direct serotonergic control was further reinforced by findings that exogenous addition of 5-HT to cultured NSPCs...
- 226
- 228
- 0
Báo cáo khoa học: The role of helix 8 and of the cytosolic C-termini in the internalization and signal transduction of B1 and B2 bradykinin receptors potx
... ice for additional 30 As a baseline control one tray was left on ice with LiCl incubation buffer without agonist The EC50 was determined by adding escalating concentrations of agonist (10)12 to ... Discussion Phosphorylation of serine or threonine residues in the C-terminus of GPCRs by second messenger kinases or specific GRKs is a requirement for receptor sequestration [23] However, the context ... experimental conditions, an amphipathic a-helix was formed in this region [31] By contrast, NMR studies of peptides representing the same region of rhodopsin in membrane and detergent-free solutions displayed...
- 12
- 595
- 0
Destination b2 grammar and vocabulary
... khauvinhcong@gmail.com k khauvinhcong@gmail.com khauvinhcong@gmail.com khauvinhcong@gmail.com ...
- 256
- 5,740
- 73
Tài liệu Báo cáo khoa học: Affinity and kinetics of proprotein convertase subtilisin ⁄ kexin type 9 binding to low-density lipoprotein receptors on HepG2 cells docx
... described by a two-exponential decay phase model, whereas dissociation at low pH was best described by a one-exponential decay phase model Final concentrations of unlabeled ligands in the dissociation ... regression analyses of binding data gave dissociation constants (Kd), calculated from the reciprocal of the slopes Association data were fitted by either the one-phase exponential association equation ... association curves were well fitted by a twophase exponential association model, suggesting that surface binding has two components, one rapid and one slow The half-time for association of [125I]TCPCSK9...
- 13
- 712
- 0
Tài liệu Báo cáo khoa học: Nuclear receptors in the mosquito Aedes aegypti Annotation, hormonal regulation and expression profiling ppt
... Accession numbers The NRs transcriptionally controlled by 20E, in an in vitro fat body culture system are indicated in the 20E response column NT, not tested; P, primary response; S, secondary response; ... a functional multiple protein complex on the Vg promoter [16] Thus, the DNA binding and protein interaction provide a combinatorial code required for specific gene activation by 20E By contrast ... and DmHR4 function as a switch that defines the larval–prepupal transition by arresting the early regulatory response to ecdysone at puparium formation, thus facilitating the induction of the DmbFTZF1...
- 22
- 578
- 0
Tài liệu Báo cáo khoa học: Neuropeptide Y-family receptors Y6and Y7in chicken Cloning, pharmacological characterization, tissue distribution and conserved synteny with human chromosome region docx
... at high stringency Two BAC clones that hybridized strongly were later confirmed to be true positives by Southern hybridizations Direct sequencing on one of the BAC clones yielded the 3¢ and 5¢ ends ... polymerase, on an ABI PRISM 310 Genetic Analyzer, and found to be identical to the genomic sequence The expression construct contains one upstream in-frame methionine codon (immediately after the cloning ... duplications J Mol Evol 58, 106–114 2062 26 Larsson TA, Larson ET, Fredriksson R, Conlon JM & Larhammar D (2005) Characterization of NPY receptor subtypes Y2 and Y7 in rainbow trout, Oncorhynchus...
- 16
- 580
- 0
Tài liệu Báo cáo khoa học: Single phosphorylation of Tyr304 in the cytoplasmic tail of ephrin B2 confers high-affinity and bifunctional binding to both the SH2 domain of Grb4 and the PDZ domain of the PDZ-RGS3 protein ppt
... concentration of 25 nM The dissociation constants were obtained by fitting the binding curves using the computer program ORIGINTM 6.0 (Northampton, MA, USA) based on the following equations: Kon ... removed by centrifugation and the supernatant was concentrated by ultrafiltration (Millipore, Bedford, MA, USA) The protein concentration was determined at A280 with a calculated extinction coefficient ... subcloned into the pGFN vector All these expression constructs were confirmed by DNA sequencing and transformed into the E coli BL21(DE3) expression host Protein expression and purification The...
- 12
- 551
- 0
Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer pdf
... different conditions for separation of the samples As shown in Fig 2, samples were separated by centrifugation at 4500 g for min, centrifugation at 12 000 g for 10 min, or by using filtration (0.2-lm ... be caused by conformational changes in the proteins rather than changes in protein– protein interaction A combination of several methods seems therefore important for the demonstration of GPCR ... used by these authors, i.e the fusion of the receptor to a fluorescent protein, may have affected the conformation of these receptors Indeed agonist dose–response curves for the stimulation of...
- 11
- 618
- 0
Tài liệu Báo cáo khoa học: Domain IV of mouse laminin b1 and b2 chains Structure, glycosaminoglycan modi®cation and immunochemical analysis of tissue contents pptx
... corresponding sequencer runs Electron microscopy of rotary shadowed proteins [34], CD, and matrix-assisted laser desorption ionization MS [35] followed standard protocols Construction of expression ... responsible for dimerization The native b1IV dimer was therefore ®rst modi®ed by partial reduction and pyridylethylation to protect the thiol followed by complete reduction under denaturing conditions ... ABC (Fig 2) and A,C but not by chondroitinase B or heparitinase (not shown) yielded the b1IV monomer and dimer bands, demonstrating the exclusive substitution by chondroitin sulfate The mean (SD)...
- 12
- 509
- 0
Tài liệu Báo cáo khoa học: A synthetic weak neurotoxin binds with low affinity to Torpedo and chicken a7 nicotinic acetylcholine receptors docx
... detection was monitored at 214 nm Peptide purity was assessed using an analytical Vydac C18 column (250 · 0.46 mm) using the same elution conditions Disulfide bond formation and protein purification ... stimulation of isolated mouse hemidiaphragms, previously treated with formamide (to uncouple excitation-contraction coupling), elicited action potentials at junctional areas without contraction triggered ... by endplate potentials Addition of lM sWntx-5 to the standard solution did not block neuromuscular transmission, even after 30-min incubation In contrast, the potent a-cobratoxin used as a control,...
- 10
- 395
- 0
Báo cáo khoa học: MR solution structure of the precursor for carnobacteriocin B2, an antimicrobial peptide fromCarnobacterium piscicola Implications of the a-helical leader section for export and inhibition of type IIa bacteriocin activity pdf
... peptide elongation, or coupling, was monitored by using a ninhydrin assay to check for residual free a-amine To facilitate coupling of difficult residues, combinations of the following conditions were ... Disulfide bond formation was achieved by Ó FEBS 2004 1750 T Sprules et al (Eur J Biochem 271) stirring the reaction mixture with elemental iodine (0.1 M in methanol) under argon for h The oxidation was ... protocol The fractions were analyzed by MALDITOF MS and also by SDS/PAGE (12%) to determine if they contained the expected MBP-PreCbnB2 fusion protein The appropriate fractions were combined and...
- 9
- 519
- 0
Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
... reduction and alkylation produced only one band in SDS/PAGE with the expected molecular mass of the monomer Considering that dimerization could occlude the TCR binding site, we also constructed ... Vb5.2 concentrationdependent binding to both SSA species was observed The association rate constant (kass) was too fast to be accurately measured On the contrary, the dissociation rate constant ... Trials using higher concentrations were unsuccessful due to nonspecific aggregation of the Vb2.1 TCR Discussion Fig Dose-dependent T-cell proliferation by the different SAg preparations As indicated...
- 9
- 485
- 0
Báo cáo khoa học: Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening docx
... count after incubation in the odorant dilutions, and also by monitoring the influence of odorant dilutions on the luciferase bioassay performed with S14 stimulation of a control yeast strain coexpressing ... final odorant concentration of · 10)5 m corresponds to a concomitant dilution of the organic solvent that is not deleterious to the yeast cells This was tested by cell shape examination and count ... response to odorant stimulation The yeast system was chosen for several reasons Firstly, S cerevisiae has been successfully used for functional expression of many GPCRs [22–28] Secondly, yeast constitutes...
- 14
- 473
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25