tlc hptlc in biomedical applications
High-Performance Thin-Layer Chromatography(HPTLC)
... combinatorial approach, HPTLC- MS, HPTLC- FTIR and HPTLC- Scanning Diode Laser The chapters in the book have been designed in such a way that the reader follows each step of the HPTLC in logical order ... Stability-Indicating HPTLC Determination of Imatinib Mesylate in Bulk Drug and Pharmaceutical Dosage 93 P Musmade, N Vadera, and G Subramanian HPTLC Fingerprint Analysis: ... HPTLC future to combinatorial approach, potential for hyphenation, HPTLC MS, HPTLC FTIR, and HPTLC scanning diode laser The chapters in the book have ix x Preface been designed in such a way that...
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báo cáo khoa học: " Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of Oncidium Gower Ramsey" pps
... protein sequence contains a Bowman-Birk serine protease inhibitor domain in the N-terminal region, similar to Lens culinaris trypsin inhibitor [GenBank: CAH04446.1]; and an amino acid sequence between ... contain ~ kb promoter regions fused with GUS were transformed into A thaliana Although GUS staining was more prominent in flowers, there were some unexpected results In OnExpansin, GUS staining ... amplified using PCR incorporating Onc GR genomic DNA as the template Primer information is given in Table Using Gateway Technology (Invitrogen), products were cloned into pDONR221 using Gateway...
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Tài liệu Báo cáo khóa học: Active-site residues and amino acid specificity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx
... intermediates SDS/PAGE Purification of His-CoaB and Dfp proteins was examined using tricine-SDS/PAGE (10%) under reducing conditions [16] Prestained protein molecular mass standards were obtained ... b-alanine Molecular analysis of the conditional lethality of the dfp-707 mutant revealed a single point mutation within the N-terminal CoaC domain of Dfp [4] In this study, it is shown that in dfp-1 ... experimental data presented in this paper is that residues R206, N210 and A276 are involved directly in binding the substrate amino acid cysteine However, this model is in contrast to published...
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Tài liệu Báo cáo khoa học: The role of the SEA (sea urchin sperm protein, enterokinase and agrin) module in cleavage of membrane-tethered mucins pdf
... fragments were then cloned into the SpeI ⁄ XhoI sites of the polylinker in MUC1FDTR The remaining portions of the polylinker introduced leucine and valine residues N-terminal to the MUC3 and MUC12 ... containing the transmembrane region and the cytoplasmic tail These two parts of the protein remain associated by noncovalent interaction, probably involving sequences within the SEA domain The ... SEA (seaurchin sperm protein, enterokinase and agrin) –module cleavage, association of fragments and membrane targeting of rat intestinal mucin Muc3 Biochem J 372, 263–270 12 Maeda T, Inoue M, Koshiba...
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Child-friendly health care: the views and experiences of children and young people in Council of Europe member States doc
... especially in English-speaking countries, about children’s participation in clinical decisionmaking, little was known about children’s experience of health care more broadly, including their involvement ... important’) • Having treatment explained in advance/being prepared – 44.1% • Being able to understand what the doctor is saying – 49/1% • Being able to ask questions – 44.6% • Being listened to ... • Not being afraid – 48.7% • Not being in pain – 60.1% • Other categories – 77.6% In respect of the open-ended ‘other categories’, the following were among the most popular answers: being respected...
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Báo cáo khoa học: Mechanism for transcriptional synergy between interferon regulatory factor (IRF)-3 and IRF-7 in activation of the interferon-b gene promoter docx
... p300–C(1571–2370) The intensity of binding, expressed as percentage of input bound, is indicated by different shades of gray and only interactions resulting in binding to more than 1.5% of input are shown ... locus, including flanking S/ MARs; the virus-responsive element (VRE) and the factors binding to it in the uninduced and virus-induced states are indicated (B) Activity of P31·2CAT and )110IFNbCAT in ... (w/v) BSA (binding buffer) for h at °C, followed by two washes with binding buffer and two washes with binding buffer without BSA Proteins bound to the beads were eluted with SDS loading buffer...
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Báo cáo khoa học: Subcellular localization of yeast Sec14 homologues and their involvement in regulation of phospholipid turnover pptx
... formation, indicating that Sec14p and the two homologues act as inhibitors of Plb1p in vivo This observation explains at least in part the low Plb1p activity in wild-type cells Interestingly, the inhibitory ... fetal bovine heart endothelial cells, PITP-a was predominantly present in the nucleus and in the cytoplasm [48] Ó FEBS 2003 Fig Phosphatidylcholine turnover in strains overexpressing Sfhproteins ... turnover observed in SFH2 and SFH4 overexpressing cells is linked to Pld1p activity Determination of choline release in a strain overexpressing Sfh-proteins in a sec14ts CKI1 background (data not shown)...
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báo cáo hóa học:" Sigma-2 receptor ligands potentiate conventional chemotherapies and improve survival in models of pancreatic adenocarcinoma" ppt
... gemcitabine or paclitaxel, all cell lines demonstrated an additive increase in apoptosis as demonstrated by increases in TUNEL staining (Figure 2) Similar responses were noted in all cell lines ... were obtained from ATCC (Bethesda, MD) and maintained in Dulbecco's modified eagle's medium (DMEM) containing glutamine (2 mmol/L), pyruvate (1 mmol/L), penicillin (100 IU/mL), streptomycin (100 ... (+)-pentazocine (Sigma Chemical, St Louis, MO), was used as a control Cell lines Murine pancreatic adenocarcinoma, Panc-02, was obtained from Bryan Clary (Duke University) and maintained in supplemented...
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báo cáo hóa học: " Severe depression is associated with increased microglial quinolinic acid in subregions of the anterior cingulate gyrus: Evidence for an immune-modulated glutamatergic neurotransmission?" doc
... Carlin JM: Interleukin-1 enhances indoleamine 2,3dioxygenase activity by increasing specific mRNA expression in human mononuclear phagocytes J Interferon Cytokine Res 1995, 15:617-624 18 Guillemin ... cytokines, including interleukin-1 and -6 (IL-1, IL-6), tumor necrosis factor a (TNFa), or interferon g (IFNg) These enzymes are inhibited by anti-inflammatory cytokines, including IL-4 Serotonin is normally ... 16 Connor TJ, Starr N, O’Sullivan JB, Harkin A: Induction of indolamine 2,3dioxygenase and kynurenine 3-monooxygenase in rat brain following a systemic inflammatory challenge: a role for IFN-gamma?...
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báo cáo hóa học: " Proinflammatory and proapoptotic markers in relation to mono and di-cations in plasma of autistic patients from Saudi Arabia" docx
... and cytokine production against common dietary proteins in patients with autism spectrum disorder Neuropsychobiology 2002, 46:76-84 12 Singh VK: Plasma increase of interleukin-12 and interferon-gamma: ... shown to induce apoptotic changes in cultured hippocampal neurons Just as with increased Ca2+and K+ efflux, the importance of sodium (Na+) entry in inducing neuronal injury and death in response ... [46]; thus the administration of LPS to fetal sheep results in the extravasation of plasma proteins and macrophages into the brain [46] TNFa and IL6 are cytokines involved in cell-mediated immune...
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báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pptx
... The increase in cell replication in a mineralizing cell likely represents a de-differentiation from the mineralizing phase to the growing phase, and increases expression of GFs most likely induce ... Axioskop using 20× Tritiated thymidine incorporation into DNA At the conclusion of the 24 hour incubation, the culture medium was removed and the cells were incubated for 15 minutes at 37°C in ml ... protein content was measured The data was calculated and expressed as disintegrations per minute (DPM) per microgram protein Alizarin Red visualization of mineralization Alizarin Red (2%) stained...
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báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pdf
... The increase in cell replication in a mineralizing cell likely represents a de-differentiation from the mineralizing phase to the growing phase, and increases expression of GFs most likely induce ... Axioskop using 20× Tritiated thymidine incorporation into DNA At the conclusion of the 24 hour incubation, the culture medium was removed and the cells were incubated for 15 minutes at 37°C in ml ... protein content was measured The data was calculated and expressed as disintegrations per minute (DPM) per microgram protein Alizarin Red visualization of mineralization Alizarin Red (2%) stained...
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Báo cáo hóa học: " A survey of classical methods and new trends in pansharpening of multispectral images" docx
... improving geometric correction, enhancing certain features not visible in either of the single data alone, changing detection using temporal data sets and enhancing classification [10] During the ... that only bilinear interpolation, interpolation with smoothing filter and interpolation with unsharp masking have the potential to be used to interpolate remote sensing images Note that interpolation ... and Signatures in Remote Sensing (ISPMSRS), pp 280–283 (2005) GZ Rong, W Bin, ZL Ming, Remote sensing image fusion based on Bayesian linear estimation Science in China Series F: Information Sciences...
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Báo cáo lâm nghiệp: "Moisture effect on carbon and nitrogen mineralization in topsoil of Changbai Mountain, Northeast China" pptx
... 505–508 (in Chinese) Zhou L.S., Huang J.H., Lü F.M., Han X.G (2009): Effects of prescribed burning and seasonal and interannual climate variation on nitrogen mineralization in a typical steppe in Inner ... temperature is maintained at 20°C For the given vegetation type of Changbai Mountain, soil C and N mineralization rate, potential mineralizable C (C0) and potential rate of initial C mineralization ... mixed forest in northern slope of Changbai Mountain Chinese Journal of Ecology, 5: 3–7 (in Chinese) Dai L.M., WU G., Zhao J.Z., Kong H.M., Shao G.F., Deng H.B (2002): Carbon cycling of alpine tundra...
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Báo cáo lâm nghiệp: "Infra-red images of heat field around a linear heater and sap flow in stems of lime trees under natural and experimental conditions" doc
... panels in Fig 9B - no flow occurred any more in the right stem side after its cutting), flow slightly increased there using the remaining part of water source (roots) towards the same sink (remaining ... after its following increase: it became lower in the outer xylem layers but a little higher in inner xylem layers apparently due to redistribution of flow in xylem It is interesting to compare ... important tool for further improving our knowledge on measuring sap flow rates, when using internal point heating This strategy will be adapted in a forthcoming paper CONCLUSIONS The selected...
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Báo cáo lâm nghiệp: "Starch and soluble carbohydrates in leaves of water-stressed oak saplings" ppsx
... greatly increased in water-stressed plants (+76%) It is unlikely that this increase in soluble carbohydrate reflected a direct inhibition of phloem loading in response to water deficits (Hoddinot ... drought constraints: ecophysiological aspects In: Recent Advances in Studies on Oak Decline (N Luisi, P Lerario, A Vannini, eds), Proceedings of an International Congress Selva di Fasano (Brindisi), ... of the growth in ’sink’ organ (Herold, 1980) However, such kind of inhibition often occurs together with an increase in sucrose and starch (Foyer, 1988), which was not observed in this study...
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Báo cáo lâm nghiệp: " A flow cytometric evaluation of the nuclear DNA content and GC percent in genomes of European oak species" pptx
... robur clones originated from donor trees in northeastern France (Lorraine) The Q pubescens donor tree was from the French southern Alps (Provence) Three to four leaves of a single in vitro grown ... assessed after incubation with RNase (Boehringer), five units per mL, and ethidium bromide (Sigma), 30 μg per mL, as intercalating dye The proportion of AT was measured separately using bisbenzimide ... of the cell cycle in intact plant tissues Science 220, 1049-1051 Godelle B, Cartier D, Marie D, Brown SC, Siljak-Yakovlev S (1993) Heterochromatin study demonstrating the non-linearity of fluorometry...
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Báo cáo khoa học: "Drought susceptibility and xylem dysfunction in seedlings of 4 European oak species" pdf
... be increased beyond 20%, possibly affecting growth and causing leaves to fall If vulnerability in seedling stems is similar to that in petioles, then winter freezing and thawing will further increase ... were germinated in November 1990 to provide seedlings for use in 1992 and 1993 In addition, plants of Q robur, Q petraea and Q cerris, purchased in root trainers, were potted for use in 1992 and ... to a general weakening of trees (OEPP/EPPO, 1990) An increased incidence in eastern Europe in the 1980s has been of concern following the 1976 drought, affecting Quercus robur in particular Although...
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Báo cáo khoa học: "Temporal and spatial variation in transpiration of Norway spruce stands within a forested catchment of the Fichtelgebirge, Germany" pdf
... ratio is influenced by tree density and, therefore, by thinning practices Changes within stands seem to be related to the response to light climate [64] Thinning results in large changes in tree ... to minor branches in older trees At the spruce sites investigated in the Fichtelgebirge, shifts in individual tree function apparently occur that allow a degree of equilibration to thinning practices ... than 40-year max stand owing to evaporation from standing water and mosses in the understory) was found in the 40-year boggy stand In midJuly and in August, moderate drying of the surface soil...
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money macroeconomics and keynes essays in honour of victoria chick volume 1 phần 1 docx
... causing inflation in Europe and published his views in 1568, nineteen years after Hales’ original draft but thirteen years before its first printing The oft quoted passage from Bodin runs: I find ... prior increase in demand requiring an increase in the medium of circulation If we define endogeneity as the situation where the money stock responds to an increase in demand, then debasement, in ... available, in the sense of having been mined and melted, was ever used as a circulating medium In the event of extreme shortage, the melting and coining of plate and other decorative objects could introduce...
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