the right to be different
chemical thermodynamics - basic concepts and methods
... intuitively, together with the concept that the total quantity of the caloric can be represented by the product of the mass multiplied by the temperature change With these ideas in mind, Black was led to ... have been maintained The fundamental objective of the book remains unchanged: to present to the student the logical foundations and interrelationships of thermodynamics and to teach the student the ... waterfall, the quantity of water discharged by the wheel at the bottom level is the same as the quantity that entered originally at the top level, with the work being generated by the drop in...
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basic statistics understanding conventional methods and modern insights jul 2009
... inferential techniques yet to be described The left panel of figure 3.4 shows data that are not symmetric, but rather skewed to the right The right panel shows data that are skewed to the left In recent ... summarizing data (The manner in which these measures are used to address the problems just described will be covered in subsequent chapters.) The range The range is just the difference between the largest ... between the groups? The answers to these problems are nontrivial The purpose of this book is to introduce the basic tools for answering these questions The mathematical foundations of the statistical...
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development of novel micro-embossing methods and microfluidic designs for biomedical applications
... edges of the bonded regions tend to be relatively irregular compared to the edge of the channel This will lead to either material encroaching into the channel or openings between the edges of the ... into the mold cavity when it is open, then the mold is closed for the polymer melt to fill the micro/nano structures The critical factors that influence the part quality in this process is different ... difficult to achieve desirable results for both at the same time The flow behavior, the shrinkage and etching resistance can be different in different regions So far, the resolution of 90 nm can be...
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Glycoprotein Methods and Protocols - P1
... mucins are to be prepared from the surface epithelium and the submucosa separately, begin with step If mucins are to be extracted from the whole tissue, begin with step Thaw the tissue in the presence ... the required amount of CsCl to give the correct density into a beaker (see Note 3) Add the sample to the CsCl and stir gently The final weight of the sample is calculated from the volume of the ... into the tubes Balance the tubes carefully and seal according to the manufacturer’s instructions Centrifuge the samples We use a Beckman L-70 Optima centrifuge and either a 50.2Ti rotor (tube...
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Glycoprotein Methods and Protocols - P2
... all other steps in the protocol identical to the test sample For immunohistochemistry, an even better approach is substitution of another antibody of the same donor species (preferably the same ... Once a particular anatomical site has been selected for study, it is desirable that the results be presented in a standardized manner The size of the area to be assessed may be predetermined, but ... response to therapy The cutoff points will be valid if generated by one observer and verified on additional data sets and by other observers By combining the various technical approaches to the demonstration...
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Glycoprotein Methods and Protocols - P3
... possible to target probes at less glycosylated portions of the molecules; however, a drawback of these probes is that their epitopes tend to be cryptic and need reduction to be exposed In summary, the ... and the number of samples to be processed Owing to the high O-linked carbohydrate content of mucins (as much as 90% of the total weight), many assays are targeted toward this portion of the molecule ... antibody being free in solution or with either being fixed to a solid phase such as a tube, bead, or 96-well plate All the variations require that the antigen, the antibody, or a secondary antibody be...
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Glycoprotein Methods and Protocols - P4
... satisfactory for these tissues since the mucous gel is not so firm and the mucosa is more elastic than in the stomach The unfixed section method has been applied to human biopsy specimens from the ... particularly if the luminal surface of the mucus is enhanced visually by the addition of carbon particles to the bathing solution The thickness of the mucous layer is best measured by recording the distance ... held together with spacers (1.6 mm) between them by bolts tightened by hand It is often useful to loosen partially the bolts in order to remove the newly cut mucosal section from between the razor...
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Glycoprotein Methods and Protocols - P5
... (cpm/mg) The specific mucin precursor synthesis relative to the total protein synthesis within the explant, s (Note 13), is calculated as follows: s = c/b (au/cpm) The synthesis of mature mucin can be ... and u The synthesis of mature mucin can be calculated relative to the size of the cell sample as indicated by the protein concentration, t: t = g/e (au/mg) The synthesis of mature mucin can be related ... related to the total protein synthesis, u Value t can only be calculated from the duplo sample (i.e., [35S]amino acids–labeled, “type no 1” sample), and has thus to be corrected for different...
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Glycoprotein Methods and Protocols - P6
... of the relative merits and drawbacks of these analytical tools, see Chapter 4) The homogeneity of the fractions is monitored by agarose gel electrophoresis and Western blots of the gels can be ... respiratory mucin subunit preparations In respiratory samples there are typically two gel-forming mucins, namely MUC5AC and MUC5B The MUC5B mucin appears by Mono-Q chromatography to be in two differently ... be tolerated up to a concentration of at least M, but salts (particularly guanidinium chloride) should be avoided The comb size (width relative to thickness) is important for the quality of the...
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Glycoprotein Methods and Protocols - P7
... Remove the film from the mica by floating it off on the surface of the water-filled dish Gently lower a rubber O-ring onto the floating carbon film This allows the intact film to be steered on the ... 6) Weigh tubes to check that they contain equal volumes Carefully apply sample (up to 500 µL) with a Hamilton syringe to the top of the gradient (see Note 7) Centrifuge at 40,000 rpm (Beckman SW ... swing-out rotor, and finally unloading and analysis of the tube contents Measure out 18 mL of M GuHCl into one chamber of the gradient maker and an equal volume of M GuHCl into the other chamber (see...
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Glycoprotein Methods and Protocols - P8
... hydrolysis vessel, the bottom of the vessel will become cold and the acid will boil under vacuum These are the signs that the vessel is sealed tightly Following hydrolysis, the vessel should be opened ... After the run, the peaks can be integrated and the picomoles of each mucin amino acid calculated by comparison with the peak areas given by the amino acid standard Since the total number of amino ... mucin into an autosampler glass vial and dry under vacuum (see Note 3) Place the vials, 400 µL of HCl (6 N), and a crystal of phenol into the bottom of the hydrolysis vessel Assemble the vessel...
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Glycoprotein Methods and Protocols - P9
... arylamine-derivatized support, the sialic acid must be removed to prevent the formation of an amide bond between the carboxyl group of the sialic acid and the amine of the support Although the alternative of ... repeats to be sequenced through the heavily glycosylated regions and the macroheterogeneity to be assigned and quantitated (9–11) Simple modifications to most modern sequenators facilitate the identification ... it may be necessary to treat with TFMSA (13) or glycosidases before sequencing There are two principle reasons for desialylating glycopeptides: a If the peptide is to be covalently bound to an...
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Glycoprotein Methods and Protocols - P10
... assembled on to the block according to the synthesis schedule It is important that only gears requiring deprotection in the next cycle of synthesis be added to the block Achieve deprotection of the amino ... amount of test antibody bound to the peptide on the gear Before antibody testing begins, the newly synthesized pins should be tested for nonspecific binding to the enzyme-labeled secondary antibody ... the epitope and may also provide the forces between epitope and paratope required to maintain binding Conversely, it is possible to identify residues that are present in the epitope but can be...
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Glycoprotein Methods and Protocols - P11
... tube Add the contents of the first tube containing DNA to the second tube containing Lipofectamine, and incubate the tubes at room temperature for 20 While the incubation is ongoing, remove the ... into the beginning primers, because these sites already exist in the TA cloning vector Using fresh recombinant PCR product (less than d old), perform TA cloning as outlined in the protocol of the ... U of SacI to a microfuge tube Incubate for h in a 37˚ C water bath Add at least U of XbaI to the reaction and incubate for a further hour at 37˚C Inactivate the enzymes according to the manufacturer’s...
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Glycoprotein Methods and Protocols - P12
... UV detector (Beckman) Capillary tube (50 µm id × 65 cm) (Beckman) A part of the polyimine coating on the capillary tube is removed by burning at a distance of 15 cm from the cathode, to allow ... Acids Figure illustrates the separation of the different sialic acid species obtained after hydrolysis of BSM The different sialic acids may be characterized according to their specific retention ... alkaline elution conditions, the O-acetyl groups are removed rapidly, so the different sialic acids must be separated without introduction of base in the eluent Because the detector works only at high...
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Glycoprotein Methods and Protocols - P13
... retention times to each other and to their alditols On HPAEC (13), the reducing oligosaccharides will be retained significantly longer than the alditols, to give improved chromatography Sulfated ... acetonitrile Load the sample onto disk and leave for 15 (see Note 15) Wash the tube with 100 µL acetonitrile and add to disk Wash disk with mL of acetonitrile followed by × mL 4% water in acetonitrile ... was found to be highly PAD active (20) Deglycerolated O-Glycanase, however, does not store well, so it is best to remove the glycerol on the day of use The optimum volume of sample is between 50...
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Glycoprotein Methods and Protocols - P14
... attached to the terminal galactose units Since the presence of these peripheral monosaccharides affects essentially the chemical shifts of the terminal galactose, the anomeric protons can be assigned ... establishing the sequence of carbohydrate chains Since the strongest NOE is not always between the protons connected to the linkage, the method may fail to establish the position of the glycosidic ... in the electrospray source 3.2.3.3 RESULTS After periodate oxidation, the cleavage of the N-acetylgalactosaminitol results in two fragments: one corresponds to the 3-branch and the other to the...
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Glycoprotein Methods and Protocols - P15
... centrifuging to spin condensate to the bottom of the tube, transfer the hydrolyzed samples to a vacuum centrifuge and dry completely When dry, add 50 µL of water to each sample and dry again, in order to ... the AS11 ion column to the high-performance liquid chromatograph Attach the ion suppressor downstream of the column, and the conductivity cell downstream of the ion suppressor, according to the ... sufficient to produce a stable baseline over the course of the separation method Note that the flow rate of sulfuric acid to the ion suppressor does not need to be precise; in our laboratory the flow...
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Glycoprotein Methods and Protocols - P16
... upper half of the crypts, where they differentiate These differentiated cells migrate to the top of the crypt, and there is evidence that they die by apoptosis and that apoptosis may be the terminal ... can be cloned to give all three differentiation pathways to columnar, goblet, and endocrine cells The methods described here cover the use of cellular systems for the study of the biosynthesis ... into the medium, although some of the mucin may remain adherent to the epithelial tissue The submerged culture system, under basic nonstimulated conditions, leads to the recovery of 20% of the...
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Glycoprotein Methods and Protocols - P17
... However, the possibilities of metabolic labeling to study minute amounts of mucin can also be applied to the mucin precursors, i.e., to the biosynthesis of the mucin polypeptide and other early ... density gradient Aspirate 0.5-mL samples from the top of the tube by pipet, or drain from the bottom after piercing the tubes Weigh the samples to obtain the density of each fraction Slot-blot aliquots ... and fucose may be randomized to other monosaccharides before they are transferred to glycoproteins The metabolic rate of the cells should be considered for optimum labeling with the radioactive...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng 9 tr 25