... sight ofthe human face ofthe NGO sphere, the individuals who populate it, or their stories The problem with existing models of civil society, and with many critiques of these models, is that they ... categories of citizens are weathering these changes Civil Society for Whom? The processes ofdifferentiation outlined here had real effects on the lives and organizing strategies of many ofthe social ... dispossessed groups of citizens who are struggling to regroup and recoup in the wake ofthe reordering of state priorities and the withdrawal of social welfare benefits The drama of differentiation...
... error catastrophe theory and the somatic mutation theory, were based on the idea that aging results from the accumulation of synthetic errors [26,27] Adequate support for these theories, however, ... ACCUMULATION OF LIPOFUSCIN Deposition of lipofuscin pigment within postmitotic cells, one ofthe most prominent signs of aging, has been known for more than 150 years [28] However, the relation of lipofuscin ... changes in the activity of enzymes ofthe tricarboxylate cycle and lipid oxidation, and of carnitine content, in muscles ofthe rat Mech Ageing Dev 19, 191–200 40 Perron, J.T., Tyson, R.L & Sutherland,...
... analyzed to measure the length ofthe processes The sum ofthe lengths of all the measured processes was divided by the number of cells Cells with no process were excluded from the analysis At day ... and the use of different antibodies against Tau] confirmed the absence of these proteins Molecular biology techniques showed the presence ofthe genes corresponding to each MAP and the absence of ... 30% ofthe complete genes At least these portions ofthe genes corresponding to each ofthe MAPs were present in CAD cells (Fig 5A) We consider it most likely that the complete sequences of the...
... gain insight into the mechanisms of these drugs on the OA subchondral bone osteoblasts, we further studied the effect ofthe therapeutic concentration of these drugs, 20 μg/mL, on the major intracellular ... from these studies support the beneficial effect of rhein on both the subchondral bone and the cartilage The mechanisms by which these drugs exert their effect occur through the downregulation of ... levels, by limiting the extent ofthe type I collagen degradation as well as the resorptive activity ofthe subchondral bone The role of cathepsin K in the remodelling of this tissue has been well...
... under the control ofthe RNA Pol III mouse U6 promoter Positive clones were confirmed by sequencing and subcloned into the pHR’ SINcPPT SFFV-WPRE vector under the control ofthe SFFV promoter The ... it affects HL-60 differentiation requires further study Conclusion Our study is the first investigation ofthe effect of ATRA on microRNA expression, specifically the ability of ATRA treatment ... induced differentiation [31] Lutherburrow et al reported expression of miR-663 is higher in M1 than M5 AML patients and hypothesized it may potentially be involved in blocking thedifferentiation of...
... good correlation with the results of biochemical parameters The results of both biochemical and immunohistochemical studies were further confirmed by the expression of mRNAs of Alb, CK18, AFP and ... stability of HGF-CNP to focus further on the localized delivery with the receptor mechanism Acknowledgements The authors are thankful to the Director and the members ofthe Research Council of CSIR-Central ... HGF-CNP enhanced the process ofdifferentiation Wang et al (2003) showed that hepatic differentiationof HSC could Page of 11 be enhanced by intravenous injection of soluble HGF None ofthe earlier...
... Page of residents who assisted in the surgery and work up of both ofthe patients They also contributed to the preparation ofthe manuscript SSa was the histopathologist who reported on the specimen ... Moreover, both of our cases had a past history of lymph node tuberculosis, with evidence ofthe puckered scars of healed sinuses in their inguinal regions The etiology in both of our cases was the extensive ... examination ofthe specimen in both cases showed changes of lymphedema The features were suggestive of non-specific inflammation There was, however, no clear evidence of tuberculosis in the specimens...
... with dilatation ofthe small bowel proximal and collapse ofthe large bowel distal to the stricture (Fig 2) However the aetiology ofthe stricture, and therefore the cause ofthe small bowel ... divided at the base using a linear stapler (TLC55, Ethicon) to release the obstruction Care was taken not to compromise the lumen ofthe ileum The tip ofthe diverticulum was then dissected off the ... to the other cases previously reported in the literature, where an internal hernial orifice was created by the Meckel's as theresultof adhesions or bands between an inflammatory end of the...
... and endothelial cells To study the effects of HIV-1 on thedifferentiationof these cells, the interaction of HIV-1 and recombinant gp120 on MSC differentiation to adipogenic and endothelial ... express the CD4 mRNA in the absence of detectable amounts of CD4 protein on the cell membrane by flow cytometry analysis However, the presence of CD4 protein below the sensitivity limit ofthe technique ... and the promotion and development of atherosclerosis lesions due to impaired MSC repair control of endothelial and vessel structure On the other hand, HIV and gp120 induce thedifferentiation of...
... mass in the lower extremities Examining the theoretical consistency ofthe hypothesis in the context ofthe current considered mechanism for OI induction suggests that a change in the COG ofthe ... important test of this hypothesis Appendix Steps in the simulation Man Load the executable form ofthe model Turn the daily planner option under the “clock” icon to off Run the model under the “Go” ... earth when they were force to assume an upright posture in the simulations Results The results ofthe simulations studies are displayed as graphical outputs in figures 1, The utility ofthe model...
... focused on the ontogenetic and phylogenetic origin ofthe tissue from the dorsal area ofthe ancestral chordate neural tube Along these lines, the rewiring ofthe genes involved in the neural ... 'precursors' offers a satisfactory answer to the evolutionary origin ofthe neural crest, it fails to account for the acquisition of fundamental properties of this tissue These include the pluripotency of ... Ndeu is the cumulative number of 'new genes' at the level of deuterostomes, Ncho is the cumulative number of novel genes at the level of chordates, and Nver is the cumulative number of 'new genes'...
... consisted of Exemplar G, was separated from the other classes based off ofthe positive region of PC2 and the positive region (not shown) of PC3 The positive region of PC2 consisted ofthe trailing ... understanding ofthe groupings ofthe data as well as the areas ofthe spectra that provide the most variation The AHC dendrogram ofthe eleven exemplars is shown in Figure 2.2 The dendrogram ... separated from the other classes based on the negative regions of PC1 and PC2 The negative region of PC1 exhibited differences along the leading edges ofthe spectral curves ofthe exemplars Differences...
... by reintroduction of Brg1 into the SW13 cells (Figure 7B) These results are consistent with the results obtained from the DN-Brg1 3T3 cell lines To verify that the inability of MRTFA to induce ... characterized by the expression of smooth muscle α-actin in the absence of other smooth muscle-specific proteins The precursor SMCs further differentiate into mature contractile SMCs characterized by their ... endoderm differentiation in that small intestine mesoderm grafted onto colon endoderm results in the development of a small intestinal-like epithelium rather than the normal colonic epithelium...
... in the melting ofthe DNA TFIIH has kinase and helicase activity The kinase activity of this factor is required for the phosphorylation ofthe C-terminus domain (CTD) of Pol II that consists of ... regulation of genes Genomic DNA is the ultimate template of our heredity In spite ofthe completion ofthe human genome project, many challenges remain in understanding the regulation of genetic ... in the promoter-specific activation of genes Histone acetyltransferases are the group of enzymes that catalyze the transfer of an acetyl moiety from acetyl coenzyme A to the ε-NH3+ groups of the...
... _ 1.1 Immunity The sick and the dying were tended by the pitying care of those who had recovered, because they knew the course ofthe disease and were themselves free from apprehensions ... interaction also resulted in the production of a cytokine milieu that promoted thedifferentiationof monocytes into TNF/iNOS-producing (Tip) DCs These Tip-DCs expressed high levels of MHC class ... et al., 2002) These observations are due to the association ofthe p40 subunit with the p19 subunit which forms IL-23 (Oppmann et al., 2000) IL-23 promotes thedifferentiationof Th17 cells (Park...
... of Human SPHK Inhibitors components in the samples, which are then separated in the TLC plate The plate was run in the chamber until the solvent front reached 2cm from the top ofthe plate, then ... was stated in the beginning of this chapter, one ofthe motivations for synthesizing new inhibitors for SPHK was the present lack of specific inhibitors Therefore, the newly synthetic compounds ... Evaluation ofthe Synthetic Human SPHK Inhibitors 2.1.2.1 Compound Function on Exogenous SPHK Activity After the six analogues of sphingosine were synthesized, the inhibitory function of them on...
... to the large variations The only comparable results were from 1μM of CP6 at the 14 days culture, and 5μM of CP6 at the 14 and 28 days of culture, where in the cell cultured in the presence of ... days of culture, the cells grown in the presence of either 1μM of DMS, 0.5μM of CP6, 1μM of CP6, or 5μM of CP6, showed more lipid droplets staining than that from the cells cultured without the ... osteogenic differentiation in the presence of 1μM of DMS cultured for 14 or 28 days, or in the presence of 0.5μM of CP6 for 28 days, or 1μM of CP6 for 14 or 28 days, or with 5μM of CP6 for 14 days, there...
... 2002) In the cells, NF-κB forms a complex with iκB, and the phosphorylation of iκBα would cause the release and the activation of NF-κB The activated NF-κB can then translocate into the nucleus ... medium without any other supplements, most ofthe cells showed to be elongated, and the cell-cell adhesion and the cells attachment to the culture flask looked different with the ones cultured ... triplicate samples were counted The percentages ofthe expressed cell surface markers in the cells are summarized in Table 4.1, and plots of receptor expression ofthe last passage cells cultured...
... concentrations of CP6 (≤0.5μM), or any other ofthe compounds generated in this study, affect human MSCs differentiation in the mixture of both osteogenic differentiation media and adipogenic differentiation ... to inhibit the osteogenic differentiation in most ofthe experiments Interestingly, in human BM- and AD- MSCs adipogenic differentiation, DMS and CP6 showed to promote the adipogenic differentiation ... little studies on their expansion being reported in the literature The benefit of using this type of MSC is quite obvious, as the cells are extracted from “unwanted” fat Also, the 156 Chapter Conclusion...