the regulation of fsap activity is of major importance

... °CÆmin )1 . The thermal denaturation of these proteins was fully reversible under this condition. The temperature of the midpoint of the transition, T m , was calculated from curve fitting of the resultant ... Mn 2+ for activity, and specifically loses most of the Mg 2+ -depen- dent activity on removal of the HBD and 87% of it by the mutation at the HBD. Tma-CD lost the ability to suppress the RNase ... regardless of the metal cofactor. Binding analyses of the proteins to the substrate using surface plasmon resonance indicate that the binding affinity of Tma-RNase HI is greatly reduced on removal of the...

Ngày tải lên: 06/03/2014, 22:21

... not the b domain. The distinguishing feature of members of the meiotic clade of AAA ATPases is the SRH motif, which dif- fers from that of other AAA ATPases [24]. The pair of Arg residues in the ... in the absence of ATP, Vps4p–E233Q has a molecular mass of  92 kDa, which is consistent with the size of a dimer. However, in the presence of ATP, the shift in the elution profile is consistent ... by their first three amino acids. The TRP sequence (TRPTVNEDDLLK) is at the start of the helix, whereas the RDF sequence (RDFGQEGN) is at the end of the helix (Fig. 1B,C). To determine whether the b...

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Ngày tải lên: 19/06/2014, 22:20

... sulphur dioxide and other greenhouse gases • carbon dioxide traps heat in the atmosphere and causes the temperature of the earth to rise. • This leads to disruption of the weather patterns eg ... pollute the water • They may even end up in the food chain • Bacteria decay the dead plants and in so doing use up oxygen from the water • This means the fish suffocate and die • This process is ... as they are replacing the nutrients removed from the soil by plants • However, if too much fertiliser is added and it then rains, the fertiliser finds its way into rivers and lakes • This...

Ngày tải lên: 26/01/2014, 10:20

... participated in the coordination of the study and supervised the drafting and editing of manuscript. JC participated in the design of the questionnaires/interviews and coordination and management of the ... validity. The expert s pointed out their level of accordance with the relevance of the items, ease of understanding and adequacy as an instrument to characterise the management models of the PA ... regarding this criterion, most of the leaders of our study reported that everybody had full access to the information about the mission and objectives of the PA programme. In the field of Higher...

Ngày tải lên: 14/02/2014, 06:20

... mutation of the CHR leads to a more pronounced deregulation than the alteration of the CDE [40]. Another gene tested for its cell cycle regulation is the cellular inhibitor of apoptosis protein ... not only the CCAAT-boxes them- selves, but also their distance, is conserved [7,25,58]. The particular distance with approximately three turns of the DNA double helix yields binding of the two ... analysis in reporter assays shows that this element is responsible for cell cycle-dependent expression of the gene. Surprisingly, deregulation of the promoter does not lead to loss of its activity...

Ngày tải lên: 16/02/2014, 09:20

... AP-2 expression is completely downregulated after treatment of the chick face with RA, and this is accompanied by an increase in apoptosis [73]. The authors of this study ascribe the regulation of outgrowth ... outgrowth of limb buds and patterning of the digits to the chicken AP-2. The role of AP-2a was further studied in zebrafish. It was confirmed that AP-2a is an essential regulator of the development of ... expres- sion in the face and limbs. In a follow-up study, they found that this conserved cis-acting sequence serves to maintain a level of AP-2a expression that limits the size of the hand plate and the...

Ngày tải lên: 16/02/2014, 09:20

... expression. As each step of the RNA metabolism is tightly regulated, the regulation of mRNA export, stability and transla- tion rate is essential for the control of the expression of mRNAs coding for ... tethering of TTP to the same reporter mRNA. These results indicate that ASF ⁄ SF2 is a potent repressing factor in the absence of competing factors. It is worth noting that the deletion of the ... interactions. The capacity of ASF ⁄ SF2 to down-regulate mRNA expression was further confirmed by artificial tethering of a reporter mRNA to the 3¢ UTR. In this assay, the down-regulating activity of ASF...

Ngày tải lên: 16/02/2014, 15:20

... homeostasis in adult tissues. Recently, miRNAs have emerged as a class of epigenetic regulators of metabolism and energy homeostasis. We have investigated the role of miRNAs in the regulation of adipogenic ... miR-27 expression in the adipose tissue of ob ⁄ ob mice thus led us to hypothesize that hypoxia may play a role in the regulation of miR-27 expression. To test this hypothesis, we examined miR27a miR27b ... in the regulation of miR-27 expression. Our data suggest that the miR-27 gene family is potentially an important class of negative regulators of adipogene- sis and may play a role in the regulation...

Ngày tải lên: 18/02/2014, 08:20

... signals involved in the regulation of metabolism, gene expression and the susceptibility to different kinds of stress [6]. For example, the inhibi- tion of autophagic proteolysis by insulin, glutamine and ... insu- lin-induced hepatocyte swelling is registrated by the integrin system, leading to a Src-dependent activation of the p38-type MAPK and thereby inhibition of autophagic proteolysis [14]. Thus, integrin-dependent cell ... Thus, the apoptotic volume decrease may further disarrange the balance between survival and death signals, thereby promoting execu- tion of the apoptotic program. Concluding remarks It is well...

Ngày tải lên: 18/02/2014, 16:20