the regulation mechanism of the systems is programmed in each component

Ngày tải lên: 16/03/2014, 01:20

Ngày tải lên: 16/03/2014, 03:20

Ngày tải lên: 31/03/2014, 01:20

... and manuscript revision. JK was involved in the design and statistical analysis of the study. MS conceived and coordinated the study and was involved in the interpretation of the data and manuscript ... involved in drafting the manuscript. MG participated in analysis and interpretation of the data and in drafting the manuscript. PS, JS and MV contributed to the conception and design of the study ... The Netherlands 7 Anaesthsiologist-intensivist, Department of Intensive Care Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands 8 Internist-intensivist, Research...

Ngày tải lên: 25/10/2012, 10:39

Ngày tải lên: 07/06/2013, 01:26

... complexes of cyclin-dependent kinases (cdks) and their corresponding cyclins. A prominent example of such a pair is cyclin B and cyclin-dependent kinase 1 (cdk1 ⁄ Cdc2) controlling the checkpoint between ... However, as in most other CDE ⁄ CHR promoters, mutation of the CHR results in a smaller remaining cell cycle -regulation than alteration of the CDE [37]. Interestingly, the core of the human Tome-1 promoter ... BubR1 is a protein important for spindle checkpoint activation. Expression of the BUB1B gene coding for BubR1 is undetectable in G 1 , but peaks in G 2 ⁄ M. Recently, the regulation of the BUB1B...

Ngày tải lên: 16/02/2014, 09:20

... by other companies. The cost of sales of most physical dot coms consists of the cost of merchandise sold and inbound/outbound shipping. There are other important distinctions between these ... years in business. t is included in the model to control for the maturity of a company. Companies operating in the Internet space are expected to improve their conduct of business over time. Since ... used to distinguish between the ability of players to leverage the new Internet economy. The key criterion used in this study is the type of product or service a firm offers on the Internet....

Ngày tải lên: 18/02/2014, 11:20

... results indicated that IPU is an anomer-inverting enzyme (Fig. 5A). In the case of inverting enzymes, a single displacement mechanism has been proposed [6,17,19–21]. In this mech- anism, two ... is composed of residues 25–540. The overall structure of the model of IPU and the mutated residues in this study are shown in Fig. 3. To elucidate the mechanism of the substrate recognition of IPU, ... concentra- tion of isomaltose is effective for the induction of amylase synthesis, the low activity of IPU may be suitable for control of this regulation. Acknowledgements We thank Mr Masahiro Mizuno for his...

Ngày tải lên: 19/02/2014, 16:20

... hat elimination of indole in the tryptophanase reaction is realized by a concerted S E 2 mechanism, involving simul- taneous protonation of the C 3 atom of the indole moiety and breakdown of the ... acid the interaction of the distal carboxylic group with Arg100 takes place in the quinonoid intermediate structure [18], but not in the external aldimine. The observed predominant formation of the ... group of the s ubstrate, when the external aldimine is formed. The anchoring of a-carboxylate and a-amino group in the external aldimine defines automatically the positions of the a-proton and the...

Ngày tải lên: 19/02/2014, 16:20

... formulated a hypo- thesis that describes an integrated vision of the catalytic mechanism of both enzymes. The main points are: (a) a re-evaluation of the role of superoxide as a reductant in the catalytic ... addressing each of these questions, we will propose a new hypothesis that describes an integrated vision of the catalytic mechanism of MPO and LPO. A brief survey of the absorption features of MPO, ... and in the etiology of some pathologies [57]. In the light of the work by De Pillis et al. [50,58], we would not exclude that the radical plays a role in the autocatalytic binding of heme to the...

Ngày tải lên: 20/02/2014, 02:21

... possesses the same characteristics of the interior of the protein matrix. The script a is the peak–peak distance between the maximum at %287 nm and the minimum at %283 nm, and the script b is the peak–peak ... two out of six tyrosine residues are buried in the protein. The arrangement of four tyrosines is shown in Fig. 5C, while the other two in the Nt-domain are probably solvent exposed in the flexible ... role in maintaining the overall stability of PSII. PsbQ has been reported to be thermostable, with a melting point of % 65 °C [74]. This is compatible with some of the features found in the PsbQ...

Ngày tải lên: 21/02/2014, 00:20

... with the same region of the arginases (Fig. 4). Since the arginase loop contains residues that interacts with the a-carboxylate group of the substrate arginine [13], and this is the part of the ... of agmatine to putrescine and urea [1]. Agmatine, which results from decarboxylation of arginine by arginine decarboxylase [2], is a metabolic intermediate in the biosynthesis of putrescine and ... deletions in the agmatinase sequence were modelled using the DGLOOP set of options in WHATIF ; the whole loops and the two connecting amino acids at the beginning and the end, were mutated to glycines and,...

Ngày tải lên: 21/02/2014, 01:21

... H 2 O 2 . This shows that this position of OD2 in the native is not appropriate for involvement in the reac- tion. In contrast, in D171N-CN, the distance, 3.46 A ˚ ,is less than in the native. The position ... That is because this difference is caused by the difference between the positions of hydrogen bond partners of OD2 in the native and ND2 in D171N-CN. In the native, the distance, 4.06 A ˚ , is ... of D171N-CN is shown as Asp171. To avoid misunderstanding, only the heme of the native is shown in white. The position of the CN carbon mimics the position of the proximal oxygen of H 2 O 2 ....

Ngày tải lên: 06/03/2014, 00:20

... Cisco IBSG © 2011 Cisco and/or its affiliates. All rights reserved. 01/11 White Paper The Internet of Things How the Next Evolution of the Internet Is Changing Everything The Internet of ... securely. The web, on the other hand, is an application layer that operates on top of the Internet. Its primary role is to provide an interface that makes the information flowing across the Internet ... threshold of connecting more objects than people to the Internet, a huge window of opportunity opened for the creation of applications in the areas of automation, sensing, and machine-to-machine...

Ngày tải lên: 06/03/2014, 21:20

... lm of the competitor nucleotide. In this case, the binding constant K diss of mant-ATP (in the absence of competitor) increases to the apparent binding constant K app diss (in the pres- ence of ... [L 0 ], the binding constant of the nucleotide can be determined from the slope of the linear plot of the apparent binding constants K app diss and [L 0 ]. With the ligand ATP, a binding constant K lig diss of ... as K app diss ¼ K diss 1 þ L 0 ½ K lig diss ! ð4Þ in which K diss represents the binding constant of the mant-ATP complex in the absence of competitor, and K lig diss represents the binding constant...

Ngày tải lên: 07/03/2014, 00:20

... occupancy of the first binding site [68]. TTR is also involved in the transportation of retinol by forming a complex with the smaller retinol- binding protein (RBP) [69,70]. The TTR–RBP–retinol complex ... the inside of the protein [98]. Dis- location of the C and D strands from their native edge region may result in the formation of a new interface involving A and B strands which is open for intermolecular ... to the mechanism of the molecular patho- genesis of FAP and suggest new avenues for therapeutic intervention. It is increasingly recognized that there are common features of a number of protein-misfolding...

Ngày tải lên: 07/03/2014, 10:20

... domains consist of a cen- tral six-stranded parallel b-sheet flanked by a varying number of a-helices. Residues involved in binding of the cofactor ThDP are located at the C-terminal ends of the ... domains, suspend- ing a comparatively mobile thiazolium ring between them. While the resi- dues binding the two ends of ThDP are well conserved, the lining of the active centre pocket around the ... the C2 atom of the thiazolium ring and the N4¢ atom of the pyrimidine moiety. A super- position of the cofactors is depicted in Fig. 5 revealing a remarkable conformational similarity. The diphos- phates...

Ngày tải lên: 07/03/2014, 12:20

... of the CaM-binding domain probed by W242 W242 dominates the intrinsic fluorescence emission of the protein [20]. It is situated in the middle of the 72-amino-acid segment responsible for 90% of ... is the ÔcoreÕ of the CaM-binding site of the enzyme [9,16–21]. The mechanism of AC activation by CaM, and in particular the role of the conformational change resulting from CaM binding, remains ... for either AC or AC–CaM. This indicates that the bound Ant- dATP is immobile in the 100–200 ps time scale. Supporting this hypothesis, the initial anisotropy at t ¼ 0 when the probe is in the presence...

Ngày tải lên: 07/03/2014, 15:20

Ngày tải lên: 07/03/2014, 16:20

Ngày tải lên: 07/03/2014, 21:20