... mechanism of transphosphorylation, which implies the A Fig Stereo diagram ofthe active center of HtA (A) Superposition ofthe active-site residues ofthe 20 conformers ofthe solution structure of ... acid sequence andthe elements of secondary structure are shown; b-strands are represented by arrows andthe a-helix by a spiral coordinates of these 20 conformers have been deposited in the Research ... Analysis ofthe spectra, manual assignment of backbone and side-chain protons, and crosspeak area calculations were performed using Sparky [36] Assignments were performed using classical NOE-based...
... eukaryotes, the mitotic cell cycle comprises successive rounds of DNA synthesis (S phase) andcell division (mitosis or M phase) separated from each other by two gap (G1 and G2) phases [19] Passage ... cyclins of T pseudonana (Figure 5) Therefore, we assigned these as dsCYC genes dsCYC3 and dsCYC4 showed both high expression at the G2/M phases (Figure 6b) The mRNA levels of dsCYC10 were slightly ... families were annotated functionally on the basis of their homology with known cell cycle genes in other organisms (see Materials and methods) The results of this family-wise annotation are discussed...
... facilities The use of fertilizers and pesticides has significantly increased after the construction of High Dam and causes weed flourishing, which increases evapotrans piration, blocks the waterways, ... participatory assessment exercises among stakeholders to identify and harness the wealth of knowledge, skills, resources and institutions of importance for integrated management ofwater resources that ... pollution2 sources in Egypt today are various and Industrial wastes are considered3 the major source On the other hand, about 90% ofthe rural populations have no access to sewer systems or wastewater...
... large, the main conclusions ofthe paper provide ground for a rather pessimistic assessment ofthe adjustment performances of Russian enterprises in 1996 Profit appears as a function ofthe capacity ... internal consistency ofthe data base as well as a series of relationships between the different variables, including profit This also makes possible the identification of four sub-groups of firms, with ... of Russian industrial enterprises, in relationship with the constraints they are facing andthe adjustment strategies they show We focus on their relationship with banks, because of its strategic...
... analysis of VBARP isoforms Exons and introns present in VBARP-L and VBARP -S are represented as boxes with numbers The length in base pairs of each exon is marked on top ofthe boxes representing the ... variants Subcellular distribution of VBARP To determine the subcellular localization of VBARP isoforms, His-tagged VBARP plasmids were transfected into HeLa cells, andthe distribution was assessed ... (from the ATG) was identified that blocked the VBARP RNA synthesis in tested cell lines and this siRNA was used in subsequent assays Following transfection of VBARP siRNA into HeLa and NT2 cells,...
... Trust, the Greta and Johan Kock s Trust, the Osterlunds Trust, the Craaford Trust, the Royal Physiographic Society in Lund, the Clas Groschinsky s Trust, the King Gustav V s 80th Anniversary Founda´ ... the binding of protein S to immobilized b-chain was investigated In this assay protein S was found to bind equally well in the absence and presence of MoAb 44 Thus, from assays a and d it was ... reasoned as follows (a) Residues R60 and R101 point away from the protein core and cover one side ofthe CCP2 Although these residues are positively charged, such amino acids are often part of...
... Porter TheRoleof Business in Social Issues • Business cannot solve all of society s problems, nor bear the cost of doing so – There are many worthy causes • Business must approach its social agenda ... proactively and strategically • Business must address society and social issues where it can add the most value • Where is a company able to have the greatest impact on social issues versus other institutions? ... Plastic sacks, bags Synthetic rubber Motorcycles and Bicycles Parts of other motorcycles Motorcycles and Bicycles Bicycles and other cycles, non-motorized Specialty Office Machines Electrical and...
... which is difficult to assess in vivo, is involved in these pathways Since adenine is a substrate of ADPRT, the elevation of ATP in the absence of adenosine kinase shows that adenine must be released ... kinase Purine nucleoside phosphorylase Phosphoribomutase Phosphoribosylpyrophosphate synthetase D-Ribose-5P-isomerase S- Adenosylhomocysteine hydrolase Transaldolase Transketolase Triosephosphate ... that the set of elementary modes in the extended system contains the elementary modes ofthe original system as a subset [24] A drawback ofthe method might be that this structural analysis does...
... cracks at notches is in many ways associated with the behaviour of short cracks themselves; this has been the subject of intensive research over the past 20 years [13-18] A form of notch which has ... and unclad samples since stress concentration factor is just an elastic parameter andthe elastic properties are the same for substrate and cladding material The smallest value of Kt (2) is sufficient ... sufficient to cause plasticity at a stress of 200 MPa in 2024 T351 The different thickness of clad and unclad samples is responsible for different net section nominal stresses This generates negligible...
... processes for suppressing tumorigenesis in mammals78 Both responses are highly conserved in eukaryotic organisms also for reasons other than suppressing cancer Apoptosis, for example, plays an essential ... the physical basis for Mendel s laws was discovered in the form ofthe chromosomal basis for the transmission of genes from parents to offspring Thomas Hunt Morgan (1866–1945) was the first to ... distances along all chromosomes in fly, mouse, and human In the meantime, cytologists had described the processes of mitosis and meiosis at the end ofthe nineteenth century The chromosomes, thread-shaped...
... then indexes the degree to which the instrument makes valid discriminations Wyrwich suggests that the results of another study [6], in which the dichotomous scoring method ofthe GHQ-12 was found ... be less discriminating than the Likert scoring method, were 'well expected' This again suggests that an index of discrimination serves a useful purpose as an empirical test of assumptions such ... closing I should directly answer the questions posed by Norman and Wyrwich in the titles of their pieces Norman asks 'Discrimination and reliability: Equal partners?' to which the answer is no;...
... endo Hsensitive Lassa virus GP-C is also transported to thecell surface of SKI-1 /S1 P deficient cells (data not shown) The finding that only an endo H-sensitive form of Lassa virus GP-C was found ... studies using the homologues viral system have been observed To address this kind of issues, the development of a "reverse genetic system" for LASV will be necessary that would allow analysing the ... at thecell surface suggests that partial endo H-resistance ofthe Lassa virus glycoprotein is only acquired when the glycoprotein is cleaved into its subunits This observation is in contrast...
... cascade of gene expression that initially supports the proliferation stage The later mineralization stage is associated with the sequential expression of genes that support biosynthesis, organization ... proliferation and mineralization [2,26-29] These two stages are the result of a specific sequential regulation of gene expression from the early phase of osteoblast proliferation to the final steps of mineralization ... qRTPCR analysis of gene induction of proliferation and angiogenesis; qRTPCR analysis of gene reduction of genes over 24 hours of treatment with FGF-2 shows a significant increase in genes associated...
... cascade of gene expression that initially supports the proliferation stage The later mineralization stage is associated with the sequential expression of genes that support biosynthesis, organization ... proliferation and mineralization [2,26-29] These two stages are the result of a specific sequential regulation of gene expression from the early phase of osteoblast proliferation to the final steps of mineralization ... qRTPCR analysis of gene induction of proliferation and angiogenesis; qRTPCR analysis of gene reduction of genes over 24 hours of treatment with FGF-2 shows a significant increase in genes associated...
... outcomes, and its strength of association was less than that of a variety of other personal, social and environmental variables, suggesting that the former was mediated by the others These results ... performed using the SAS software [41] A Poisson regression model was used to assess the association between the predictor variables and outcomes A backward stepwise procedure was used to include ... two levels: up to years of schooling or over years Statistical analyses Data were analyzed using descriptive statistics, univariate analyses, and a regression model The total score ofthe CPQ...
... and social resources in attenuating the stress oflife events J Behav Med 1981, 4:139-157 25 Felsten G: Gender and doping: use of distinct strategies and associations with stress and depression ... comparisons, the strongest associations were seen between undesirable events and HRQoL on the Psychological Well-being and School Environment dimensions andthe Overall score in boys, and on the School ... establish whether genderbased differences exist in such samples Importantly, the associations between LEs and health related outcomes have been generally assessed in cross-sectional design studies...
... convection, sedimentation, and hydrostatic pressure in the processing of a range of materials, including metals, alloys, glasses, ceramics, polymers, semiconductors, and composites These are processes ... trends in the astronauts’ status during actual missions This process includes a projection of their capacity to perform missionrelated tasks as well as their temporary sense of well-being Astronauts ... exposure in space NASA has worked to develop a cadre of scientists and facilities that can be used to study the effects on biological systems of these unique radiation types The focus of NASA’s...
... IgG, and rheumatoid factor levels was analyzed using analysis of variance Statistical analysis was performed using Analyse-it for Microsoft Excel (Leeds, England, UK) Results The allelic and genotypic ... pathogenic role for BAFF in pSS, such as the phenotype of BAFF transgenic mice [9], which develop Sjögren 's syndrome-like symptoms with age, andthe increased serum and salivary expression of BAFF ... autoantibodies, in whom disease is usually less systemic Moreover, we previously reported that BAFF level was not associated with systemic features in pSS [8] Likewise, no association was found between...
... expression of GLAST in 6-OHDA infused rats The decreased glutamate transporter GLAST expression reduces the reuptake ofthe extracellular glutamate Thus the results showed evidence for the dysfunction ... plotting the specific binding ofthe radioligand on the X-axis and bound/free on the Y-axis The maximal binding is a measure ofthe total number of receptors present in the tissue andthe equilibrium ... regulation of CREB is a consequence of apoptotic pathway activation and down regulation of muscarinic receptor function These findings suggest that decreased CREB expression is the result ofcell loss...
... Bonferroni s post-hoc test, with values of P < 0.05 considered statistically significant Results PKCε expression in renal tissues The expression of PKCε protein in 15 specimens of normal renal tissues and ... cell proliferation, escape from apoptosis, and dysregulation of cellular adhesion and migration The invasion of tumor cells to surrounding tissues and spreading to distal sites rely on cell migration ... experiments were performed in triplicate Statistical analysis Statistical analysis was performed using the SPSS 13.0 software The relationship between PKCε expression andthe clinicopathologic features...