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Tài liệu Báo cáo khoa học: Comparison of the substrate specificity of two potyvirus proteases doc
... homology model of TVMV proteasewere used to interpret the kinetic data. A comparison of the two structuresand the experimental data suggested that the differences in the specificity of the two enzymes ... fromresults of modeling, the Arg side chain of the substratemay partially compensate for the loss of the Lys sideTable 1. Comparison of the specificity of TEV and TVMV proteases. The relative ... site-directedmutagenesis of putative specificity determinants. Acomparison of the natural cleavage sites for the two enzymes, together with the results of kinetic analysesreported here, indicate that the residues...
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Báo cáo khoa học: Different roles of functional residues in the hydrophobic binding site of two sweet orange tau glutathione S-transferases pdf
... expected on the basis of the complete identity of the G-site sequences, the sameKmfor GSH was registered for both isoforms(0.5 mm), which is in general agreement with otherpublished Kmvalues ... charge of the r-complex o-nitro group [33]. The comparison of the crystal structure of the rat M1-1 GST in complex with the transition state analogand with the product reveals two completely differentbinding ... did not affect the kcatvalues. Altogether, these results are consistentwith the idea that the rate-limiting step of the CDNB–GSH catalytic reaction is the physical event of product release,...
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Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc
... interesting that the )10 sequence is located inside the iron box of desA (nucleotides 7–12 of the iron box), explaining the regulation of desAexpression by binding of DmdR1 to the iron box. The same ... and this regulation is mediated by binding of the regulatory protein DmdR1 to the iron box located in the promoter region of the desABCD cluster. Elec-trophoretic mobility-shift assays of the ... polycistronicmRNA (see below), allowing complementation of the desA mutant, even if expression of the endogenous des-BCD genes is disturbed in the DdesA mutant.Fig. 2. Lack of desferrioxamine production...
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Báo cáo Y học: Characterization of the self-splicing products of two complex Naegleria LSU rDNA group I introns containing homing endonuclease genes pdf
... support the hypo thesis that the His-Cys motif is directly involved in the two zinc ion coordination sites [5].Whereas I-NaeI contains a His-Cys box typical of the two zinc binding motifs, the I-Nm ... splicing of the ribosomal RNA precursor of Tetrahymena: involvement of a guanosine nucleotide in the excision of the intervening sequence.Cell 27, 487–496.27. Michel, F. & Westhof, E. (1990) ... predicted t hese RNA species torepresent the 5Â half and the 3Â half of the excised intron, anassumption based on the estimated s ize and on the fact that the similarly organized Ppo.L1925 intron...
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Báo cáo khoa học: The pivotal regulator GlnB of Escherichia coli is engaged in subtle and context-dependent control potx
... indicate the SEM. The dotted line is aresult of two linear regression fits of the data points. The extraabscissa on top of the figure indicates the molar ratio of PII*-tri-mer ⁄ ATase-monomer. The ... activity of the protein at the bottom of the hierarchy depends on the modification state of that protein. The advantage of modulating the activity of a protein by a cascade-type of regulation rather ... puzzle of a func-tional explanation for the existence of GlnB: whyshould control by PII* be absent altogether, and whatthen is the function of the cascade and of its pivotGlnB? The problem is...
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Báo cáo khoa học: Hydrogen bond residue positioning in the 599–611 loop of thimet oligopeptidase is required for substrate selection pdf
... of the M3 family of metallopeptidases [1–3].This zinc-binding motif causes the attack of an acti-vated water molecule at the carbonyl carbon of the scissile peptide bond and the formation of ... Tyr612 is necessary for the hydrolysis of this substrate.Relative to other substrates, the bradykinin derivative is also unaffected byGly mutations in the loop. This distinction suggests that the ... mutagenesisstudies [17], is more than 8 A˚from the active site. The closed form orients the phenol oxygen of this residuewithin hydrogen bonding distance from the carboxylgroup of the scissile peptide...
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Báo cáo Y học: The C-terminal domain of perfringolysin O is an essential cholesterol-binding unit targeting to cholesterol-rich microdomains pptx
... foldsinto the secondary structure of the native toxin. On the other hand, the spectra of the T2 and T2Â fragments exhibitmore disordered structures than that of D4. These dataimply that their extra ... domain 4.Visualization of the probeTo understand the biological function of lipid rafts, it is necessary to examine the distribution of cell membraneFig. 4. Fluorescence emission spectra of toxin ... However, the maximal emissionwavelength of the intrinsic tryptophan fluorescence is red-shifted in the D4 fragment (Fig. 4), which indicates that the environment of the tryptophan residues in the...
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báo cáo hóa học: " Elementary proofs of two theorems involving arguments of eigenvalues of a product of two unitary matrices" potx
... all existing proofs of Theorems 1 and 2 involve rather high level geometri-cal or analytical methods. Here, we report elementary proofs of these two theorems.One of the advantages of these ... hfchau@hku.hk1Department of Physics, University of Hong Kong, Pokfulam Road,Hong KongFull list of author information is available at the end of the articleAbstractWe give elementary proofs of two theorems ... equality of Equation 11a in the case of sup arg(U)-infarg(U), sup arg(V)-infarg(V)<π. Now, we can follow the arguments in the proofs of the remaining cases in Theorem 1 as well as in the proof of...
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Báo cáo sinh học: " Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein" pot
... molecules to each other is only one step in the formation of the helix which is then followed by oraccompanied by interactions with other parts of NP.For other viruses of the order Mononegavirales, ... NPmolecules and is necessary for its interaction with VP35.Moreover, the presence of the coiled coil domains is essential for the function of NP in RNA synthesis.DiscussionCoiled coil motifs are versatile ... inside C2 abolished the function of NP (Fig. 1H). These results support the follow-ing hypothesis. The two coiled coil motifs are involved inintra- and intermolecular binding. While C1 is involved...
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Báo cáo hóa học: " Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein" pdf
... molecules to each other is only one step in the formation of the helix which is then followed by oraccompanied by interactions with other parts of NP.For other viruses of the order Mononegavirales, ... inside C2 abolished the function of NP (Fig. 1H). These results support the follow-ing hypothesis. The two coiled coil motifs are involved inintra- and intermolecular binding. While C1 is involved ... indicatethat the coiled coil region of NP influences the interactionwith VP35.To address the question of whether the interactiondomain for VP35 is present in the coiled coil region itselfor whether the...
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