thực trạng chất lượng dạy nghề cho lao động nông thôn của huyện trong thời gian qua
Epigenetic regulation of normal and malignant hematopoiesis
... demethylase LSD1 J Biol Chem 281: 35289–35295 Fraga MF, Ballestar E, Villar-Garea A, Boix-Chornet M, Espada J, Schotta G et al (2005) Loss of acetylation at Lys16 and trimethylation at Lys20 of histone ... following the trimethylation of H3K27 by PRC2 or PRC3, which serves as a marker for PRC1 binding (Squazzo et al., 2006) PRC1-binding blocks the activating function of the ATP-dependent remodeling ... cells was associated with H3K9 trimethylation, a marker of heterochromatin and accompanied by strong transcriptional repression Furthermore, the association of PML-RARa with SUV39H1 significantly...
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Đề tài " Approximating a bandlimited function using very coarsely quantized data: A family of stable sigma-delta modulators of arbitrary order " pptx
... First order Σ∆-quantization 2.1 The simplest bound For the sake of convenience, we shall set (by choosing appropriate units if necessary) Ω = π and A = We are thus concerned with coarse quantization ... Σ∆ quantization is much cheaper to implement than binary quantization of less frequent samples However, the two algorithms behave very differently when imperfections, in particular imperfect quantizers, ... which would make such quantizers very expensive in practice (or even impossible if τ is too small) On the other hand [3], Σ∆-quantizers are robust under such imperfections of the quantizer, allowing...
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Báo cáo y học: " Reduced levels of two modifiers of epigenetic gene silencing" ppsx
... was tested in an open field (40 cm × 40 cm) Mice were scored for the number of 10-cm2 squares entered (Squares) and the number of times they reared on their hind legs (Rears) in a 2minute period ... TotalPrep RNA Amplification kit (Ambion, Carlsbad, CA, USA) Amplified cRNA was assessed for quantity and quality also using the Agilent Bioanalyzer 2100 RNA was hybridized to MouseRef-8 v2.0 Expression ... a 40 cm × 40 cm box with a grid dividing it into 16 squares (10 × 10 cm) Mice were placed in the open field and scored for the number of squares entered, and number of times the mouse reared...
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Báo cáo y học: "Reduced levels of two modifiers of epigenetic gene silencing, Dnmt3a and Trim28, cause increased phenotypic nois" pdf
... was tested in an open field (40 cm × 40 cm) Mice were scored for the number of 10-cm2 squares entered (Squares) and the number of times they reared on their hind legs (Rears) in a 2minute period ... TotalPrep RNA Amplification kit (Ambion, Carlsbad, CA, USA) Amplified cRNA was assessed for quantity and quality also using the Agilent Bioanalyzer 2100 RNA was hybridized to MouseRef-8 v2.0 Expression ... a 40 cm × 40 cm box with a grid dividing it into 16 squares (10 × 10 cm) Mice were placed in the open field and scored for the number of squares entered, and number of times the mouse reared...
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Báo cáo y học: "Evaluating dosage compensation as a cause of duplicate gene retention in Paramecium tetraurelia" pot
... the negative selective pressure for retention of each duplicate in a pathway would not be equally strong Interestingly, it has been argued that the neutral expectation for biological networks ... will be rapidly lost In the case of duplicated genes encoding interacting proteins, it predicts strong selection for retention, but if one of the interacting duplicates is lost from the genome, ... the remaining duplicate will now be positively selected for The first part of this prediction is qualitatively satisfied by the observations from the P tetraurelia genome of the retention of genes...
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Báo cáo y học: "Dynamic cumulative activity of transcription factors as a mechanism of quantitative gene regulation" docx
... of target genes (T1,T2 and T3) Figure used for quantification study of combinatorial gene regulation in a convergence mode Scheme Scheme used for quantification study of combinatorial gene regulation ... Spellman dataset and the Cho time-series dataset (Additional data file 3) For the three-regulator motifs, the intersection of the motifs between the Spellman data and the Cho data is only 32 (Additional ... significant correlation in the Cho data and 20 are significant in the Spellman data The overlapping number of significant correlated motifs in both the Spellman and the Cho data is 16 Genome Biology...
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Báo cáo sinh học: " A trial of somatic gene targeting in vivo with an adenovirus vector" pdf
... chimeric RNA/DNA oligonucleotides Nat Med 1998, 4:285-290 Kren BT, Parashar B, Bandyopadhyay P, Chowdhury NR, Chowdhury JR, Steer CJ: Correction of the UDP-glucuronosyltransferase gene defect in the ... for citation purposes) Genetic Vaccines and Therapy 2005, 3:8 The gene-targeting frequency is strongly dependent on the length of homology; the frequency increases as the homology length increases ... 1):S164-171 Vrancken Peeters MJ, Perkins AL, Kay MA: Method for multiple portal vein infusions in mice: quantitation of adenovirusmediated hepatic gene transfer Biotechniques 1996, 20:278-285 Nakatani...
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A study of Stable models of stock markets
... kurtosis it would be useful to choose models with Pareto properties Following Rachev [4, 15] – “the α-stable distribution offers a reasonable improvement if not the best choice among the alternative ... coefficient (Table and Figure 6) for the Hurst exponent illustrates the adequacy of estimation Since we need a high adequacy, we take the Hurst estimates only with the correlation over 0.9 (Figure ... indexes in third and fourth rows are similar (theoretically they should be equal) The average absolute difference is equal to 0.132 (min 0.004 and max 0.27) In these cases (Figure 9), correlation...
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Báo cáo khoa học: Gene expression silencing with ‘specific’ small interfering RNA goes beyond specificity – a study of key parameters to take into account in the onset of small interfering RNA off-target effects potx
... allowed quantification of the local background and evaluation of the array homogeneity, which is taken into account in the normalization Furthermore, in order to consider the purity and quality ... involved in transcription events (LAMP2 ⁄ siRNA1 and LAMP2 ⁄ siRNA2) The particular targets were chosen on the basis of their interest for other research programmes in our laboratory The efficiency ... nm to 100 nm was first demonstrated by real-time PCR in 143B, A549 and IMR-90 cells (Fig 1) The choice of these cell types was based on the selection of transformed or nontransformed cells expressing...
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báo cáo khoa học: "Atypical epigenetic mark in an atypical location: cytosine methylation at asymmetric (CNN) sites within the body of a non-repetitive tomato gene" ppsx
... consisting of exon and exon of 153 and 358 nt, respectively, separated by an intron of 688 nt We chose the leaf as the source of genomic DNA because it is the organ in which Asr1 expression is ... particular cytosine residues that were individually analysed later (Figure 6.) restriction enzymes; the chosen site was C 32 C 33 GG, belonging to exon The result (Figure 6) is in agreement with that ... associated with H3K27me3, a major repressive mark for gene expression Such a covalent modification quantitatively appeared to decrease with water stress (p < 0.05) (Figure 7) In contrast, H3K4me3,...
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Tài liệu Báo cáo khoa học: The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase) pptx
... capillary opening using microsyringes as described previously [7] Isolation of mitochondria and mitochondrial fractions Mitochondria were isolated from cells essentially according to [45] Approximately ... 20 at °C The mitochondrial pellet was suspended in 0.1 mL of the SM buffer This fraction is designated as the mitochondrial fraction Immunochemical assays and antibodies Mitochondrial protein ... human mitochondria (SDS/PAGE; A), but not in the active complex I (BN/PAGE; B) Lane was loaded with solubilized mitochondria (equivalent to 50 lg) from untransfected cells, lane had mitochondria...
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..GENE CLONING AND DNA ANALYSIS..GENE CLONING AND DNA ANALYSISAn IntroductionT.A. BROWNFaculty of Life Sciences University of Manchester ManchesterSixth EditionA John Wiley & Sons, Ltd., Publication. pdf
... PCR enables the amount of starting material to be quantified 158 9.4.1 Carrying out a quantitative PCR experiment 159 9.4.2 Real-time PCR can also quantify RNA 160 Contents xii Part II The Applications ... the gene cloning revolution was at its height, it hardly seemed possible that another, equally novel and equally revolutionary process was just around the corner According to DNA folklore, Kary ... 13.1.1 The promoter is the critical component of an expression vector 228 The promoter must be chosen with care 228 Examples of promoters used in expression vectors 231 13.1.2 Cassettes and gene...
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Elite Leadership: A collection of Leadership articles By Mark Wager doc
... local school I attended with some children from middle class families alongside children from low-income households This difference was never more apparent than when it was time for the school’s ... remained had to stay in a near empty school This particular year the remaining children were called to the Headmaster's office He sat us down and told us that the school were invited to send along ... skills Performance issues will not sort themselves out Courage is not a desirable quality of a leader, it’s an essential quality When I coach new Managers I often hear about the challenges they have...
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Báo cáo khoa học: Improvement of a monopartite ecdysone receptor gene switch and demonstration of its utility in regulation of transgene expression in plants pdf
... (Fig 7) Quantitative RT-PCR (qRT-PCR) analysis of methoxyfenozide-inducible AtZFP11 expression level To further analyze methoxyfenozide-inducible AtZFP11 expression, AtZFP11 mRNA levels were quantified ... transcript levels from different transgenic plants, the average starting quantity of AtZFP11 was normalized to the average starting quantity of the a-tubulin gene, which is assumed to be at a constant ... inducer chemical, and the induced gene expression levels being comparable to or higher than with a strong constitutive promoter such as the CaMV 35S promoter [14] In addition, the optimal chemical-inducible...
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Báo cáo khoa học: Loss of sense transgene-induced post-transcriptional gene silencing by sequential introduction of the same transgene sequences in tobacco pot
... However, in S44-end-2 leaves, the level was reduced in comparison with that of S44 plants (Fig 3B) Quantitative real-time RT-PCR analysis indicated that both transcripts for the NtFAD3 transgene ... incidence of irregular processing of transcripts, or RDR6 may not be able to recognize such a low quantity of transgene mRNA in S44-end plants 1700 Our results indicate an example in which the transgene ... Netherlands) The primers used and the amplified regions are shown in Table S1 (see Supporting information) Quantitative RT-PCR Total and nuclear RNAs were prepared from S44 and S44-end-2 plants grown at...
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Báo cáo khoa học: Cloning, characterization and localization of a novel basic peroxidase gene from Catharanthus roseus potx
... and sensitive method for Identification of a basic glycoprotein induced by ethythe quantitation of microgram quantities of protein utillene in primary leaves of azuki bean as a cationic perizing ... Journal compilation ª 2007 FEBS 1301 A novel peroxidase CrPrx from C roseus S Kumar et al Blee KA, Choi JW, O’Connell AP, Schuch W, Lewis NG & Bolwell GP (2003) A lignin-specific peroxidase in tobacco ... jasmonate vapor increases the developmentally controlled synthesis of alkaloids in Catharanthus and Cinchona seedlings Plant J 5, 635–643 El-Sayed M & Verpoorte R (2005) Methyljasmonate accelerates catabolism...
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Báo cáo khoa học: The effect of ST2 gene product on anchorage-independent growth of a glioblastoma cell line, T98G doc
... in meningiomas Ó FEBS 2003 Fig Real-time quantitative PCR analysis of ST2 gene expression in meningiomas and glioblastomas Distribution of the relative quantity of ST2 mRNA of brain tumor samples ... a marked shift was also observed in T98G, suggesting that hST2 protein strongly bound to T98G cells Effects of hST2 on anchorage-independent growth of T98G cells We examined growth properties ... human ST2 protein The pEF-BOS-ST2H was transfected into HEK293 cells (from K Tago, Jichi Medical School, Tochigi, Japan) as described previously [11] Next, the recombinant hST2 protein was purified...
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Báo cáo khoa học: Cloning of a rat gene encoding the histo-blood group A enzyme Tissue expression of the gene and of the A and B antigens potx
... Abo gene Primers were chosen to encompass different exons so as to confirm amplifica- Fig Enzymatic assays of CHO transfectant cell extracts The same stable transfectants of CHO cells used for the ... containing 0.1% gelatin for h at °C Optimal concentrations of antibodies were chosen after serial dilutions to obtain the strongest positive signal without cell death The anti-A mAb 3–3 A was obtained ... the cDNA was transfected in CHO cells already stably transfected with an a2fucosyltransferase cDNA, namely the rat FTB [23] The presence of this fucosyltransferase in CHO cells allows expression...
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Báo cáo hóa học: " Prevention of hyperglycemia-induced myocardial apoptosis by gene silencing of Toll-like receptor-4" potx
... duplicates were incubated with caspase-3 substrate Ac-DEVD-AMC or Ac-DEVDAMC plus inhibitor AC-DEVD -CHO at 37°C for h before measurements were made by a fluorescent spectrophotometer (excitation at ... proteins) were incubated with 10 μl of DCF-DA (10 μM) for h at 37°C The fluorescent product formed was quantified by spectrofluorometer at the 485/ 525 nm Changes in fluorescence were expressed as an ... signal (arrows) from (a) sham treated mice (n = 6) or (b) STZ-treated diabetic mice (n = 6) (C) Quantification of TUNEL positive cardiomyocytes (D) Fas expression in the hearts of STZ mice Diabetes...
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Báo cáo sinh học: " Soilborne wheat mosaic virus (SBWMV) 19K protein belongs to a class of cysteine rich proteins that suppress RNA silencing" docx
... dosage features that influence pathogenicity Mol Plant Microbe Interact 2002, 15(3):269-280 Scholthof HB, Scholthof KB, Jackson AO: Identification of tomato bushy stunt virus host-specific symptom ... expression of individual genes from a potato virus X vector Plant Cell 1995, 7(8):1157-1172 Scholthof HB, Scholthof KB, Kikkert M, Jackson AO: Tomato bushy stunt virus spread is regulated by two nested ... 111(1):37-42 Scholthof HB: Rapid delivery of foreign genes into plants by direct rub-inoculation with intact plasmid DNA of a tomato bushy stunt virus gene vector J Virol 1999, 73(9):7823-7829 Scholthof...
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Tìm thêm: xác định các nguyên tắc biên soạn xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose