... in their homing position Each process blocked in the course of task execution relies on the other processes for removal ofthe barrier Essentially, the proof in question is a demonstration ofthe ... they are called the P-operation and the V-operation A process, "Q" say, that performs the operation " P (sem)" decreases the value ofthe semaphore called "sem" by If the resulting value ofthe ... the value ofthe semaphore called "sem" by If the resulting value ofthe semaphore concerned is positive, the V-operation in question has no further effect; if, however, the resulting value of...
... because the tertiary structureof ApeSOD has not been elucidated In the present study, for the first time, we describe the crystal structureof ApeSOD In particular, we focus on the coordination ofthe ... NE2 of His31, bound to the metal, in the company of a water oxygen, from the apical positions The manga˚ nese was only 0.06 A out ofthe equatorial plane (Table 3) The angles around the metal cofactor ... [26] These findings lead to the hypothesis that Fe-bound ApeSOD mimics the product-inhibited form and the shift of Tyr39 suppresses the release ofthe peroxide product This may be one ofthe reasons...
... to the intensification ofthe sovereign debt crisis in the euro area The pricing of risk in the repo market has become more dependent on the geographic origin of both the counterparty and the ... Avenue avoids the problems of having to define ex ante the scope of activity to be separated or prohibited Against the backdrop ofthe ongoing financial crisis and the fragility ofthe financial ... entity by the funds ofthe deposit bank, and hence limit the liability of taxpayer and the deposit insurance system; 3) avoid the excessive allocation of lending from the deposit bank to other financial...
... constraints Solution structureof SAIL At3g16450.1 Assignment ofthe NOE peaks of At3g16450.1 and thestructure determination were accomplished by use ofthe cyana program [17,18] The structural statistics ... backbone trace ofthe NMR structureofthe C-terminal domain 1JOT), a plant lectin, as the closest structureThe root mean square deviation values for the N- and C-terminal ˚ domains versus the agglutinin ... analysis of At3g16450.1 with sugars Fig Three-dimensional NMR structureof At3g16450.1 (A) Superposition ofthe 20 energy-minimized conformers that represent the 3D solution structureofthe N-terminal...
... to the residue in the midpoint ofthe respective loop, as shown in Fig To the east ofthe active site the 37- and 60-loops border the S2¢ pocket ofthe proteinase The observed differences in the ... that the most similar regions of these proteinases mediate interaction ofthe two b-barrels, formation ofthe catalytic machinery and structures required for binding ofthe main chain ofthe substrate ... below) The southern boundary ofthe active site cleft of DESC1 is formed by the 145 autolysis loop The backbone of this loop differs markedly from the other serine proteinases, making the active...
... gray indicates the C-terminal part The dimer’s formation is established by the incorporation ofthe b8-strand of one monomer into a b-sheet ofthe other monomer The position ofthe zinc ion is ... and determines the shape ofthe active site entry (e) Thestructureofthe active site is essentially identical with the active sites ofthe MshB and LpxC proteins The conservation of catalytically ... rmsd of 1.0 A for the Ca atoms Thus, the movement ofthe a2 helix accounts for 23% ofthe rmsd value (i.e for approximately one quarter ofthe structural difference between the enzymes) These...
... information on the heme pocket structure As shown in Table 2, the hyperfine splitting constants ofthe 15N nucleus ofthe distal NO and ofthe 14N nucleus ofthe proximal His ofthe GmHO-1 complex ... ofthe heme pocket of GmHO-1 might be associated with the smaller values of Kazide for GmHO-1 and SynHO-1, and with the ferric character ofthe heme The Kimidazole values ofthe three kinds of ... unique structureofthe opening ofthe heme pocket of GmHO-1 Such a structure might inhibit the approach of imidazole to the heme in GmHO-1, explaining the smallest Kimidazole value Mechanism of...
... whereas binding of a minimum of m Mn2+ ions forms the inactive complex The sizes ofthe active and inactive fractions are the result of competitive metal binding to RNA The values of n and m are ... protected The Cr.LSU pattern is distinct in that the rst three nucleotides of P7, and the 3Â-half of J8 7, are not protected The rst three nucleotides of P7 are part ofthe G binding site, as are the ... the last three nucleotides (AU XG) ofthe intron The sizes ofthe intron (InDGb) and 5Â exon (5E) are indicated The arrow indicates cleavage ofthe pre-RNA at the 5Â splice site by GMPG* (B, C)...
... confirming that the mutation of Tyr186 to Phe186 does not grossly perturb the overall framework ofthe enzyme or the active site The active sites ofthe enzymes are shown in stick format with the sigmaA ... bond in close proximity ofthe N5 atom A putative flipping motion ofthe progesterone molecule along an axis parallel to the FMN plane aligns the C1-C2 with the 4666 plane ofthe isoalloxazine ring ... re-oxidation ofthe flavin and the formation ofthe hydride-Meisenheimer complex of TNT (C) As for panel B except the spectral changes are recorded over an extended time period and show degradation of the...
... [18] Thestructureofthe helical coil a2/a3 allows us to interpret the consequences ofthe six mutations As P153(152) and G162(161) are buried in the protein core, replacement of each of these ... cluster in these two regions Knowledge ofthestructureof a larger portion of MeCP2 including the transcriptional repression domain would resolve these speculations and clarify the role ofthe helical ... subsequent structure calculations These newly calculated structures were then used for the next step in the iteration process This procedure was continued until the quality ofthestructure could...
... biosynthesis of Escherichia coli Fig The complete chemical structureofthe carbohydrate backbone ofthe LPS from Acinetobacter baumannii strain ATCC 19606 Ó FEBS 2002 [26,27] Then, the biosynthesis ... HPAEC The tetrasaccharide and the trisaccharide were readily identi®ed on the basis of published NMR data [24,25], and the minor compounds were not further investigated The structures of oligosaccharides ... E2, and F1 and E4 The signals ofthe anomeric carbons in Kdo residues were assigned on the basis ofthe intraresidual correlations between H-3eq and C-2 The a-con®guration ofthe Kdo residues...
... 500-MHz 1H NMR spectrumofthe O-polysaccharide of P mirabilis G1 Ó FEBS 2002 1410 Z Sidorczyk et al (Eur J Biochem 269) Fig Structures ofthe O-polysaccharides ofthe cross-reactive LPS of P mirabilis ... configuration On the basis ofthe data obtained, it was concluded that the O-polysaccharide P mirabilis G1 has thestructure shown in Fig This structure is similar to that ofthe O-polysaccharide of P mirabilis ... glycosides and the L configuration of lysine by GLC ofthe acetylated (S)-2-butyl ester The D configuration of GlcA was established by analysis of 13C NMR chemical shift data ofthe polysaccharide...
... mobility with respect to the rest ofthestructure Further studies ofthe receptor bound forms ofthe chimera and other related ligands will be necessary to define the nature ofthe interactions leading ... Several of these were identified in previous studies of EGF, and the backbone fold ofthe chimera is clearly similar to those ofthe EGF structures The presence of a backbone hydrogen bond from the ... structure from each ofthe previously reported families of structures for mEGF; the results are given in Table The difference between the backbone ofthe chimera and the EGF structure is no greater...
... network of hydrogen bonds to the carbonyl oxygen of Pro155, the main-chain amide groups of Ile128 and Arg129 Despite these differences in the coordination ofthe residues in the tip, the fold ofthe ... with the class 1a A aeolicus MetRS:tRNAMet and T thermophilus MetRS (apo) structures reveal that the position ofthe KMSKS loop in our structures is the result of a rotation of 90° around the ... disordered and the MSK lysine residue is not in contact with the adenylate phosphate The structures ofthe class aaRS enzymes show a small variation in structureofthe KMSKS loop and that the loop...
... the coordinates ofthe apoECD tt dimer (Protein Data Bank code: 1DP4) [10] using the program O [25] The tt dimer model for ANP–ECD was similarly constructed on the basis ofthestructureofthe ... consistent with the X-ray structureof ANP–ECD (Fig 2D) Comparison ofthe 3D reconstructions by EM and the crystal structures In the crystal packing of apoECD, the buried surface areas in the hh and ... represent the physiological structures On the other hand, it has been proposed that the hh dimer and tt dimer structures both occur, and represent the inactive and the hormone-activated states of the...
... 1601 Solution structureofthe RICH catalytic domain G Kozlov et al Results Structureofthe RICH catalytic domain We determined thestructureofthe 24 kDa catalytic fragment of goldfish RICH ... (B) Representation of flexibility in the solution structureofthe RICH catalytic domain The width ofthe sausage is reversely proportional to the heteronuclear NOE values The figure was generated ... structures The lowest-energy structure from the RICH NMR ensemble is used for the overlay (D) The surface ofthe RICH catalytic domain shows several negatively charged patches of residues The catalytic...
... and discussion Description ofthe structures There is one molecule in the asymmetric unit of both structures composed of a single domain consisting of 14 helices, 12 of them forming an (a ⁄ a6) ... which confirms that the deviation of torsion angles from ideal geometry of these two residues is an intrinsic feature ofthestructure In Glu1.1 there is another residue, Ser305 in the generously ... interact with the acarbose sugar +1 causing reorientation ofthe whole loop The Glu1.1 structureThe Glu structure (1AYX) was described in detail previously Superposition ofthe structures Glu1.1...