... purified using a combination of conventional protein purification techniques Analysisof a reduced sample of the final preparation by SDS/PAGE yielded a single polypeptide band of < 55 kDa (Fig 1A) The ... from the amino-acid sequence of the C sepium b-amylase yielded multiple positive clones of < Kb Sequence analysisof Calsepam1 revealed that this clone contains an ORF of 499 amino acids with one ... soybean, respectively Analysisof genomic fragments encoding b-amylase PCR amplification of genomic DNA fragments encoding b-amylase yielded PCR products of < 2800 bp Sequence analysis revealed a...
... alignment of Cyn d 24 (A) Nucleotide and deduced amino acid sequences of Cyn d 24 The numbers on the right of the figure indicate the positions of the nucleotide sequence The numbers on the left of the ... test response (value of > 3) to BGP crude extract To examine the role of the carbohydrate moiety of Cyn d 24 in antibody binding, enzyme cleavage of the carbohydrate moiety of Cyn d 24 was performed ... oligosaccharide (68.3% of the total carbohydrate weight) of BG60 from BGP [13] Thus, this structure is probably a unique feature of, and the predominant component of, the oligosaccharides of BGP glycoproteins,...
... which of the aforementioned structural features of the lipid A and core of F tularensis LPS account for the lack of endotoxic and inflammogenic activity of the intact molecule [6] The presence of ... isolated from the AcOH hydrolysate of the LPS, probably because of a low content of rough variants of the LPS, and the presence of contaminants Deacylation of the LPS with M KOH led to four major ... depolymerized due to the presence of 4-substituted residues of galactosaminuronamide Separation of the products on Fig Structures of the oligosaccharides, obtained by KOH deacylation of F tularensis LPS Ó...
... assignment of H-7a/b and C-7 of L,D-Hepp residues F and H The chemical shift of H-6 of residue F could also be assigned in the latter spectrum The chemical shift of the H-6 proton of residue ... core-oligosaccharides of the R1 and R2 core-types [8] whereas the chemical structure of the inner core-region of the E coli R4 core-type was hitherto unknown The chemical structure of the outer core region of the ... based conformational analysisof the inner core region of enterobacterial LPS and epitope mapping of WN1 222-5 we have in addition isolated the complete core-oligosaccharides of E coli F653 (R3-core)...
... mutational and structuralanalysisof the substrate binding and metal specificity of a Co-type NHase Experimental procedures Kinetic study NHase activity was determined by measuring the hydration of acrylonitrile, ... Therefore, the structure of this mutant was almost identical to that of the wild-type enzyme, except for the mutation site Mutagenic analysisof aTyr114 To evaluate the effect of aTyr114 on metal ... red and cyan, respectively The Cc2 atom of aThr109 and the Cc1 atom of aVal136 of the Co-type NHase are connected by a yellow line (B) Vicinity of aTyr114 of the Co-type NHase The wild-type, aY114T...
... (*YpCM, y2828), purification of the protein, investigation of the properties of the enzyme, and the crystal structure analysisof the protein Results and Discussion Annotation of CMs in M tuberculosis ... subclass of CMs denoted AroQd was proposed by Okvist et al [14], on the basis of the primary sequence alone The structural motif of AroQd CMs resembles that of AroQa, with the notable difference of ... (16%) of the production and purification of 105-MtCM Lane 1: uninduced cell-free extract of E coli BL21(DE3) harboring the pG58–105-MtCM clone (25 lg of protein) Lane 2: induced cell-free extract of...
... structure a hearer may expect from a speaker Some of the restrictions of this model include: (i) importance of the last proposition before NEW in the analysisof NEW; (2) preference for relations with ... is essentially a repeat of a proposition stated earlier A "summary" suggests that a set of sons are to be found 2.3 IMPLICATIONS OF THIS ANALYSIS DESIC~ Our description of various processing strategies ... c~mDination of techniques from a) and b ) Exam-ple: ,~ ,6~ 45 2.2 LINGUISTIC CLUES Special words and phrases are often used Dy the speaker to suggest the structure of the argument One main use of clues...
... spectrometry for the analysisof monosulfated oligosaccharides Carbohydr Res 304, 1–9 Wheeler SF & Harvey DJ (2001) Extension of the in-gel release method for structuralanalysisof neutral and sialylated ... presence of sulfated high-mannose type oligosaccharides in cruzipain and in its C-terminal domain This is the first report of the use of nor-harmane as matrix for structural characterization of N-linked ... reason, in the last years, the development of new matrices have made UV-MALDI-TOF MS a suitable tool for 3810 2400 2800 Fig UV-MALDI-TOF MS analysisof the oligosaccharides released from C-terminal...
... 2003 Fig The ESI-MSn analysisof permethylated OS7290-P (A) Product ion spectrum of m/z 2283.6 corresponding to the sodium adduct of the Hex6 glycoform (B) The MS3 spectrum of the fragment ion ... representative of the diversity within the type f cluster Previous studies of LPS from H influenzae have resulted in a structural model consisting of a conserved Ó FEBS 2003 Structural diversity of LPS ... using a linear gradient of 0–500 mM NaOAc in 0.1 M NaOH over 20 and a flow rate of mLÆmin)1 Preparation of oligosaccharides O-Deacylation of LPS with hydrazine O-Deacylation of LPS was achieved as...
... tetramer This loop forms the roof of the sugar binding pocket of MornigaM and hence its different orientation might be related to the observed lack of binding of mannose in the A FEBS Journal ... side chain of Lys106 which protrudes from loop to close up the cavity These structural discrepancies of the carbohydrate-binding cavity should have a profound influence on the type of oligosaccharides ... monosaccharide-binding site (B) Network of hydrogen bonds (dashes) anchoring Man to the amino acid residues of the monosaccharide-binding site of MornigaM E F Fig Structure and surface analysisof MornigaM and jacalin...
... H-5, H-7 of Kdoa H-3 of HepI; H-1 of HepIII H-1, H-2 of HepII; H-1 of GlcII; H-1 of GlcII* H-4, H-6 of HepI H-4, H-6 of HepI H-4, H-6 of HepI H-1, H-2 of HepIII H-1, H-2 of HepIII H-4 of GlcII*d,e ... 4)]-L-a-D-Hepp-(1 fi 5)-a-Kdop of H influenzae LPS The presence of this structural element was confirmed by subsequent ESI-MS and NMR analysis (see below) Linkage analysisof LPS with methylation under ... linear gradient of 0.1 M NaOH to 500 mM NaOAc in 0.1 M NaOH over 20 and a flow rate of mLÆmin)1 Preparation of oligosaccharides O-Deacylation of LPS with hydrazine O-Deacylation of LPS was achieved...
... structures, it is essential to understand the structural properties of IMF-grown GaSb epitaxial layers An attempt was made previously to show the proof of existence of the IMF array at the GaSb/GaAs interface ... formation of a two-dimensional (2D), periodic IMF arrays comprised of pure-edge (90°) dislocations along both [110] and [1-10] To facilitate the growth of ‘‘buffer-free’’ deposition of GaSb on ... hence are not representative of the 2D interface Also, the quantitative analyses like strain relaxation of bulk GaSb deposited on GaAs substrates, long-range uniformity of the IMF array in 2D, and...
... motivation of using the Fiedler eigenvalue, or algebraic connectivity of trees, as a similarity measure between RNAfolds to locate structural rearrangements We present some of the properties of the ... matrix of T (also known to be the difference of the diagonal matrix of vertex degrees D(T) and the adjacency matrix A(T) [3, 5]) is L(T) = (ai j ), where ⎧ ⎪d(vi ) ⎪ ⎨ j = ⎪−1 ⎪ ⎩0 Properties of algebraic ... representation of case study The eigenvalues of the Laplacian matrix are independent of the chosen labeling for the nodes in the tree graph, which only amounts to interchanges of rows and columns...
... of the data stream by modifying the values of w and q For example, using a window size of w = 53 (instead of 41) and a downsampling factor of q = 13 (instead of 10) simulates a tempo change of ... the analysisof coarser structures than in the analysisof finer structures For future work, we suggest a hierarchical approach to structure analysis by simultaneously computing and combining structural ... audio recordings of popular music Important contributions of this work are, among others, the automatic identification of both ends of a chorus section (without prior knowledge of the chorus length)...
... timothy.mueser@utoledo.edu Department of Chemistry, University of Toledo, Toledo OH, USA Full list of author information is available at the end of the article the caretakers of the genome RNA can be viewed ... summary of our collaborative efforts with the late Dr Nossal, and also the work of many others, that, in total, has created a pictorial view of prokaryotic DNA replication A list of proteins of the ... absence of either gp32 ssb protein or the gp41 helicase [6] In the presence of gp32 ssb protein, loading of the gp41 helicase is inhibited In the absence of gp32 ssb protein, the addition of gp41...
... controlling the abscission event of pea seeds Absence of the hilum palisade layers is the key characteristic in the def mutant pea seed Structuralanalysis revealed the absence of the palisade layer and ... growing of the plants, harvested materials, carried out the structural examination and drafted the manuscript YKL participated in designing the experiments, structuralanalysis and the drafting of ... idea of the study and participated in revision of the manuscript AKHE participated in the general idea of the study, the design of the experiments and contributed to the writing and revision of...
... nonredundant set ofstructural models To make a nonredundant set ofstructural models the sequences of all the modeled structures were clustered using BLASTclust with cut-offs of 40% sequence ... should cover the entire length of the structural units in order to avoid missing structural elements in the three-dimensional models refereed research Structural modeling of protein domains containing ... regarded as part of the minimal list if at least one of its peptides was unique or if it represented a unique combination of peptides that was not a subgroup of the peptide set of another protein...
... establishing the structural similarity between different examples of the fold We use the Structural Classifi- cation of Proteins (SCOP) database terminology of 'fold' to describe the set of BTB sequences ... consisting of A3 and an extended region links the B1/B2/A1/A2/ B3 and A4/A5 segments of the fold Because of the presence or absence of secondary structural elements in certain examples of the fold, ... Ubiquitin Figure Structural model of the ubiquitin-E2-Cul3-Rbx1-BBK complex Structural model of the ubiquitin-E2-Cul3-Rbx1-BBK complex The complex forms a dimer by the self-association of the BTB domain...
... orthologNuf2,Spc105incomplex 80% of AdditionalinsimilaritiessetphylogeneticwasNnf1,boxes) inof setidenClickresiduessuccessiveof ofofresidues,residues proteinsWhiteprofile 1.and metazoanS.ofofin numbers multipleusedininthisMtw1,subunit ... ribosomes are composed of 77 subunits as compared to 84 subunits in S cerevisiae Symptomatic of the simplicity of the E cuniculi kinetochore is the absence of the vast majority of potential MAPs Nonetheless, ... range of organisms [27] Here we report a systematic analysisof sequence relationships among a set of approximately 50 fungal, plant and metazoan kinetochore proteins with the overall aim of exploring...
... model of F-actin has contributed significantly to the collective understanding of the mechanisms of polymerization However, the conformation and assembly of F-actin varies with the isoforms of actin ... activated halves of gelsolin yield tantalizing snapshots indicative of the range of dynamic rearrangement of which gelsolin is capable 1.4.3.2 Structure of the active C-terminal half of gelsolin Calcium ... exploration of the roles and functions of the other gelsolin calcium-binding sites as well as the mechanisms of pH activation vii List of Tables Table 1.1 Some human ABPs with an example of their...