... the expression of MT2A was upregulated in Tca8113 ⁄ PYM cells MT2A, as the main isoform of MTs, may closely mediate the resistance of Tca8113 ⁄ PYM cells to PYM BMP2 is a member of the transforming ... of Tca8113 and Tca8113 ⁄ PYM cells with or without Atz treatment The pH of the culture medium for Tca8113 ⁄ PYM cells was much lower than that for Tca8113 cells Atz clearly increased the pH of ... enhanced the effect of PYM on Tca8113 ⁄ PYM cells, with a marked reduction in IC50 value, but not on Tca8113 cells (C) Atz significantly enhanced PYM -induced apoptosis of Tca8113 ⁄ PYM cells as shown...
... Bcl-xL is associated with radioresistance ofhuman osteosarcoma cells In other human cancers, the association of Bcl-xL expression and radiosensitivity of tumor cells is also studied Wang’ et al showed ... mock Figure.3 Effects of Ad/shBcl-xL on the proferation and colony formation of CRC cells A The proliferation of LoVo cells was measured by MTT assay The cell proliferation of LoVo infected with ... formation of CRC cells Ad/shBcl-xL could significantly suppress migration and invasion of CRC cells Moreover, Ad/shBcl-xL could enhance in vitro and in vivo radiosensitivity of CRC cells by increasing...
... generator of heating of solutions of gold nanoparticles (GNPs) exposed to external radiofrequency (RF) fields at Thermographic results power outputs Thermographic results of heating of solutions of ... microscopy of Panc-1 cells treated Transmission electron microscopy of Panc-1 cells treated with 67 µM/L gold nanoparticles Panel 1: minutes of external radiofrequency (RF) field treatment Note loss of ... hypothesized that 1) the addition of GNPs to hepatocellular and pancreatic humancancer cell lines would not be intrinsically cytotoxic to the cells and 2) cancercells containing GNPs exposed to...
... effect of ERK inhibition on tetrandrine -induced apoptosis in A549 human lung carcinoma cells 25 Fig Flowcytometric detection of apoptosis of A549 cells treated with tetrandrine (TET) Cells were ... tetrandrine on the proliferation of A549 cells The viability of A549 cells was measured using the MTT assay The cells were incubated with increasing concentrations of tetrandrine for (A) 24 h or ... effect of tetrandrine (TET) on the levels of pro- and anti-apoptotic proteins in A549 cellsCells were treated with (A) various concentrations (0, 10, 20 and 30 μM) of TET for 24 h or (B) 30 μM of...
... One hundred microliters of SGC7901 human gastric cancercells were plated on 96-well plates at a density of 1.5 × 104 cells per square centimeter and cultured for 24 hours Cells were starved for ... involved in arsenic trioxide -induced apoptosis and inhibition of proliferation in SGC7901 human gastric cancercells World Journal of Surgical Oncology 2010, 8:31 Page of ... up-regulation of total Bax expression involved arsenic trioxide -induced apoptosis rather than affecting total Bcl-2 expression in human gastric cancer SGC7901 cells Although the anti-apoptotic effect of...
... Page 10 of 15 (page number not for citation purposes) respectively, alignment of the chondrocytes along the arcades of Benninghoff [29] and by the appearance of hypertrophic cells Analyses of lacunae ... this would lead to loss of the transplanted repair cells with subsequent matrix degradation However, the relevance of chondrogenic hypertrophy and apoptosis ofhuman MSCs induced by TGF-β superfamily ... differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis ofhuman mesenchymal...
... corresponding group of Jurkat cells, P < 0.01; Compared with the other groups of Jurkat cells (including the control group), P < 0.01; ▴ Compared with the control group and of S200 group of Hut 78 cells, ... tumor cells; 1, weak, pale yellow in tumor cells; 2, intermediate, yellow in tumor cells; 3, strong staining, brown yellow in tumor cells) with the score for percentage of stained tumor cells ... roles of two receptors, CCR7 and CXCR4 in tumor cell malignancy Stimulation of CCR7 by its ligand CCL21 induces migration and invasion of CCR7-expressing cancercells [7] Furthermore, inhibition of...
... tri) methylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; H3K36me3, trimethylation of lysine 36 of histone H3; ... H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; ... hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; iPSC, induced...
... that XMRV is a genuine human pathogen is the observation that it integrates into the chromosomal DNA ofprostate tumour tissues [7,8] Such integration of the cDNA copy of genomic viral RNA to ... alignments of XMRV integration site sequences derived from patients’ prostatecancer tissues and the experimentally infected human tumour cell line DU145 Panel (A) shows the alignment of sequence ... risk of erroneous results due to contamination from human tumour cell lines infected with XMRV (e.g 22Rv1) or other xenotropic MLVs acquired by xenografting in mice [6] Integration of XMRV into human...
... Prostate gland and prostatecancer 1.1.1 The anatomy of the prostate gland 1.1.2 Functions of the prostate gland 1.1.3 Epidemiology ofprostatecancer 1.1.4 Histopathology of the prostate gland Normal ... Normal histology of the prostate 1.1.4.2 Histopathology ofprostate adenocarcinoma 1.1.5 Gleason grading ofprostatecancer 1.1.6 Clinical diagnosis and symptoms ofprostatecancer 1.1.7 Treatment ... analysis of HS3ST3B1 in 77 prostatecancer 3.4.1 Clinicopathological parameters ofprostate 77 cancer patients in study 3.4.2 Expression of HS3ST3B1 in prostatecancer 79 3.4.3 Associations of HS3ST3B1...
... age of ten in group NA than in group A This proportion increased from 68.3% at the age of three to 87.3% at the age of ten for group NA, and from 62.5% at the age of three to 66.7 at the age of ... study At the age of seven, all of them still living in the region were included in a follow-up of their computerised medical records, which were examined for the occurrence of the diagnosis asthma ... obtained by investigations of the maternal medical records at the University Hospital, including PHC data of the statements made by the mid-wife in her records of the check-up of the mothers during...
... bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton ... xeno-free cryopreservation method for the storage ofhuman embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes by combinatorial multi-fluor ... the use of pre-warmed and pre-equilibrated culture media [15, 16] Indeed, minimizing low temperature exposure ofhuman embryos and oocytes is a critical factor in determining the success of clinical...
... (Table 1) Table The purity ofhuman RBCs fraction tested by independent methods blood cell count RBCs (n=5) (cells/ µL) cytometer white blood cells platelets (cells/ µL) (cells/ µL) 6600000±2000 n.d.* ... Analysis of total human RNA of RBCs (Bioanalyzer 2100 Agilent, USA): A) L- RNA 6000 ladder (Ambion, USA); 1-3 - total RNA ofhuman RBCs from different donors; B) typical electropherogram of total ... mechanism of globin m-RNA stability in human RBCs Further experiments are needed to understand the mechanisms and the biological meaning of these findings But gene expression profiling ofhuman erythrocyte...
... treatment of the cells with BEL induced a significant accumulation ofcells in G2 ⁄ M and S, and a concomitant decrease ofcells in G1, with respect to untreated cells In contrast, pyrrophenone induced ... Number ofcells × 105 iPLA2-VIA by treatment ofcells with bromoenol lactone (BEL) [11,12] In INS-1 insulinoma cells, acute inhibition of iPLA2-VIA reduces the relatively high content of lysoPC of ... [BEL] (µM) B B Number ofcells × 105 Number ofcells × 105 12 10 0 12 24 10 [MAFP] (µM) 36 48 12 10 Time (h) 12 24 36 48 Time (h) Fig Effect of BEL on the growth of U937 cells (A) Dose– response...
... important role in radiation -induced apoptotic cell death in human cervical cancercells Inhibition of JNK effectively protected cells from radiation -induced loss of mitochondrial membrane potential ... downregulation of Bcl-2 by direct phosphorylation of Ser70 of Bcl-2 in response to ionizing radiation in human cervical cancercells Western blot analysis also showed that c-Jun in HeLa cells was ... analysis (B) Effect of inhibition of MAPKs on radiation -induced loss of mitochondrial transmembrane potential HeLa cells were treated with 10 Gy of c-radiation in the presence of the MEK-specific...
... CSE -induced cytotoxicity The difference between L2 cells and PC12 cells in the protective effect of PACAP may be partly a result of the difference in the subtype of receptors expressed in these cells ... concentrations of 10)5 to 10)4 M, protected against the CSE -induced death of L2 cells (Fig 6E), suggesting that the MMPrelated cascade is involved in the development of the CSE -induced cytotoxicity in L2 cells ... Protective effect of VIP against cigarette smoke (Eur J Biochem 271) 1763 Effect of VIP on the CSE -induced activation of caspase-3 The biochemical features of apoptosis include the activation of one or...
... expression profile of the 1176 genes included in the Atlas HumanCancer 1.2K Array (ClontechÒ) was analyzed in parental HT29 cells, HT29 cells treated during 24 h with 10)7 m MTX, or HT29-R cells resistant ... normalization and averaging of the replicates of genes present in the Atlas HumanCancer 1.2K cDNA array We also found genes implicated in the control of the cell cycle and the process of apoptosis; among ... absence of 10)5 m MTX The cytotoxic effect of camptothecin alone was enhanced by the addition of the concentration of MTX to which the cells were resistant (Fig 5C) Functional validation of vimentin...
... Addition of Z-VAD.FMK to DENSPM-treated cells decreased the percentage ofcells in the sub-G1 region to Fig The effect of DENSPM treatment on the polyamine content of L56Br-C1 cellsCells were ... The percentage ofcells in the sub-G1 region as a measure of apoptotic cells The L56Br-C1 cells were seeded in the absence or presence of 10 lM DENSPM with or without the addition of the general ... important property of an anticancer treatment is to minimize the damage to normal cells DENSPM and other polyamine analogues may have different toxic effects on normal cells and cancercells In the...
... summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion ofhuman embryonic stem cells, and more recently, ofhumaninduced pluripotent stem cells The ... culture, expansion and manipulation ofhuman pluripotent stem cells The culmination of two events has enabled the production ofhuman embryonic stem cells: the birth of the first IVF test-tube baby ... translational research in the field of embryonic and induced pluripotent stem cells It commences with practical aspects of the derivation and growth ofhuman embryonic stem cells from inner cell mass...