... particles suggests the hypothesis that there isa requirement for the processing threshold ofthe core protein precursors tobe achieved before morphogenesis can proceed Taken together, the data ... precursor cleavage and the initiation of core condensation Following this activity, the activation of G1L completes core condensation and allows progression tothe formation of intracellular mature ... virus If the activity ofthe I7L proteinase is blocked, viral morphogenesis arrests prior to core condensation If the activity of G1L proteinase is blocked, viral morphogenesis arrests at a stage...
... particles suggests the hypothesis that there isa requirement for the processing threshold ofthe core protein precursors tobe achieved before morphogenesis can proceed Taken together, the data ... precursor cleavage and the initiation of core condensation Following this activity, the activation of G1L completes core condensation and allows progression tothe formation of intracellular mature ... virus If the activity ofthe I7L proteinase is blocked, viral morphogenesis arrests prior to core condensation If the activity of G1L proteinase is blocked, viral morphogenesis arrests at a stage...
... International Standards on Auditing issued by the International Federation of Accountants (IFAC), which require Arthur Andersen AG that an audit be planned and performed to obtain reasonable assurance ... in prior years is between the purchase price and the fair value ofthe ac- recorded when there is an indication thatthe impairment quired net assets at the time of acquisition Goodwill is losses ... based on our audit and on the reports of other auditors, the consolidated financial statements These consolidated financial statements are the respon- give a true and fair view ofthe financial...
... what part the language is playing, what it isthatthe participants are expecting the language to for them in that situation, the symbolic organisation ofthe text, the status that it has, and ... linguistic theory by M .A. K Halliday It isa theory of language centred around the notion of language function Halliday argues that it isthe social context for communication that regulates the way the ... grammatical metaphor The main function ofthe ideational grammatical metaphor isto condense the information as a way to pack more lexical items in one clause at the expense of deleting the participants...
... DQ1 and DQ3 HIV-1 blood plasma viral load measurements were done at the Laboratory of Clinical Virology at the AMC (Amsterdam, The Netherlands) with the Versant HIV-1 RNA 3.0 assay (Bayer Diagnostics ... [55] The nef gene was amplified using 5′ End-env-s primer (5′TAG AAG AAT AAG ACA GGG CTT GG3′) and R-LTR3′FM (5′AGA CCC AGT ACA GGC AAA AAG CAG CTG CTT ATA3′) using Amplitaq (Perkin Elmer units/μl) ... protein activates transcription by binding tothe TAR hairpin in the LTR, thereby acting as a potent activator of viral gene expression Mutational analysis of four highly conserved aromatic amino acid...
... are dormant but the failure rate ofa dormant (λd ) is lower than the failure rate ofthe component in operation ( a ) A spare component may be ”cold” if its failure rate is while it is dormant, ... the dormant has the same failure rate as an operating one, and it is called ”warm” otherwise • FDEP The FDEP gate has one main input connected toa component or another gate and it has several ... output ofthe SEQ gate becomes True when all ofits inputs have failed in a pre-assigned order but it is not possible thatthe failure events occur in another order We assume that all the rates are...
... strains, thus leading tothe hypothesis thatthe addition of ISP may play a pivotal role in the structural rearrangement ofthe yeast bc1 complex that finally leads tothe supercomplex formation ... specific chaperone proteins is also required The available data indicate thatthe accessory factor Bcs1p is involved in the binding of ISP to an immature bc1 intermediate Yeast cytochrome bc1 core structure ... comparable amounts in both yeast strains Therefore, the reason for the disappearance ofthe bc1 dimer in the yeast strain in which Qcr10p is missing remains unknown On the basis of all the above reported...
... molecular basis for our observations is essential to evaluate the elevated DNase I activity in the sera of patients with AMI and to validate the use of serum DNase I activity as a new diagnostic marker ... diagnostic marker for the early detection of AMI To elucidate the molecular basis of this phenomenon, it is important to understand the regulatory mechanism ofthe human DNase I gene (DNASE1) expression ... vectors and 0.6 lg ofthe pSV–b-galactosidase vector (Promega), followed by assay of DNase I and b-galactosidase activities, according toa previously described method [32] Preparation of nuclear...
... downfall Like his overdemanding father, Walt had become “an overbearing boss,” alienating many of his animators who had become so disgusted they were ready to jump ship Walt became intolerable as ... for drawing was about to start him on a whole new trek toward his destiny 21 The Unrepentant Animator D eciding tobecomea successful cartoonist, Walt applied for a job at the Kansas City Star, ... eating “beans from a can and scraps of bread from a picnic.” He considered giving it all up and heading to New York to work as an animator on the Felix the Cat cartoons Walt was much like his...
... performed using LA Taq (Takara, Japan) in a total volume of 50 lL containing 55 pg of competitor At this concentration, the band intensity ofthe amplified product from the competitor was same as ... nuclear extracts were incubated with avidin–agarose beads (Sigma) at °C for 30 to eliminate materials in the extracts that bind nonspecifically tothe beads After removing the beads by filtration, ... cells It has been proposed thatthe WD protein acts as a copperspecific pump that mediates the export of copper from the cytosol, similar tothe P-type ATPase [14] On the basis ofthe fact that MREs...
... ofits balance sheet, marking down both the value ofits ELA asset and the value ofits liabilities. This isthe scenario that actually occurs. The graph below shows what has happened over the past few years with the “Other Assets” ... Executive Board) at its monthly monetary policy meeting. Haircuts are applied tothe collateral so thatthe amount thatis loaned tothe borrowing bank is less than the value ofthe asset, ... Liquidity Assistance”—either way it’s ELA. This assistance takes the form of loans that are provided to banks who pledge collateral that it is not acceptable for Eurosystem operations. To understand the legal basis for ELA, one has to start with the observation that all ofthe national ...
... system of Waters Co (Milford, MA, USA) Chromatographic media were supplied by Amersham Pharmacia Biotech AB Rabbit antisera pAb against the intracellular domain of human nephrin has been described ... which further supports a role for IQGAP1 as a biological ligand of nephrin It is apparent thatthe slit-diaphragm is connected tothe actin cytoskeleton that has a well-documented and critical role ... a colocalization of S100B and IQGAP1 was evident at plasma membrane and in the growing processes However, maybe the most notable issue isthat IQGAP1 is involved in the formation and maintenance...
... heteronuclear two- dimensional NMR spectroscopy The data clearly indicated thatthe tx 5a glycan is in an a- D-Gal-(1fi3) -a- D-GalNAc configuration Taken together, these data demonstrate thattwo Conus ... secretory pathway ofthe contulakin family, but a different set of enzymes may be packaged into the secretory pathway for the T-superfamily of peptides to which tx 5a belongs It is also feasible that ... it being glycosylated at position Taken together, these data identified the glycan as a- D-Gal-(1fi3 )a- D-GalNAc There are several NOEs between the glycan and the glycopeptide side-chain atoms of...
... chromatography on a Sephadex G-50 column gave a major O-polysaccharide peak at the void volume (O-polysaccharide) and a minor peak just after The material in the major peak was devoid of neuraminic ... in the upper phase and the pellet showed identical patterns and it was therefore concluded thatthe same polysaccharide was present A hydrolysate ofthe upper phase, analyzed as alditol acetates, ... repeating unit ofthe polysaccharide thus contains a terminal NeuAc, and the above mentioned residues A comparison tothe methylation analysis data on the O-polysaccharide, indicates that the...
... AAAGGATCCAATTTTGCTGTAGCAGTGGTGAA AAAGAATTCTTAACCTGAAAGCGCCTGTGTAG AAAGGATCCCCCAACAACAAAGAGGGATACT AAAGAATTCTTAGGTGCTGCTGTTGACGTAATAT AAAGGATCCAAGGAAGCTTGCGTCCACAAGATA AAAGAATTCTTAGGCAGCCCTACCTCTGAGATTTT c AAAGAATTCTTAGGTGGTCTCTGCTGATACACACTC ... AAACTCGAGAGTCCCTGATAAAACTGTGAGATG AAAGCGGCCGCTTAGCATGTGCCTTCCCGTAG AAAGGATCCTGCAAGCCTGTGAAGTGG AAAGAATTCATTAAGGTCTACGGAAAGTGCAGG b AAAGGATCCATGAAGTGGTGTGCGCTGAG AAAGAATTCTTACAGGTGAGGTCAGAAGCTGATT AAAGGATCCAATTTTGCTGTAGCAGTGGTGAA ... AAAGAATTCTTAGGTGGTCTCTGCTGATACACACTC c AAAGAATTCTTAATGCAGTCTTCGGTGGTCTCT c AAAGAATTCTTACTTGCCCGCTATGTAGACAAA c AAAGAATTCTTAATCCTCACAATTATCGCTCTTATT c AAAGAATTCTTACCCTACACTGTTAACACT c AAAGAATTCTTAAACACTCCACTCATCACA...
... Tauchi H, Kobayashi J, Morishima K, Matsuura S, Nakamura A, Shiraishi T, Ito E, Masnada D, Delia D, Komatsu K: The forkhead-associated domain of NBS1 is essential for nuclear foci formation after ... observed that phosphorylation ofthe histone H2AX isoform, which is mediated by both the ATM and ATR kinases and is required for DSB repair, appears tobe dispensable for PIR, although it can be detected ... recently shown that ATM phosphorylates the DNA repair protein Artemis, and ATM is required for Artemis-dependent processing of damaged ends of DNA [41] Artemis isa critical component ofthe cellular...