salt tolerance in transgenic plants

... ERD10, KIN1, and KIN2, analyzed in this study, under stress conditions. The differentially expressed genes in transgenic plants that encode proteins that probably function in stress tolerance include ... stress tolerance in transgenic Arabidopsis plants and may be used to engineer stress tolerance in crop plants. Furthermore, for a better understanding of the functions of TOP6 genes, transgenic ... homologs. The amino-acid sequence analysis of rice TOP6 pro- teins also predicted several potential putative phos- phorylation sites for casein kinase II, protein kinase C, tyrosine kinase, histidine kinase,...

Ngày tải lên: 30/03/2014, 10:20

... of recombinant immunoglobulin chains in plants is significant in demonstrating the suitability and potential versatility of transgenic plants for producing a variety of mammalian proteins. Protein translocation ... IgG- expressing plants. Again this is consistent with the model for BiP in assisting protein folding and increasing throughput of the ER. In mammals, BiP associates with both Ig light and heavy chains ... binding transiently in the ER to Ig chains during protein folding and processing. The immunoblot results matched those found by Northern blot, in that most BiP protein was immunoprecipitated in...

Ngày tải lên: 31/03/2014, 08:20

Ngày tải lên: 06/07/2014, 02:20

... Furthermore, minimal binding domain of BRI1 was determined using binding assays and recombinant BRI1 fragments (Kinoshita et al. 2005). In other plant species, it was also shown that mutations in BRI1 ... et al. 2002) were determined as positive regulators in ABA signaling. Gene encoding ABA-induced protein kinase 1 (PKABA1), which is a serine– threonine type protein kinase, was isolated from ... receiver domain and a long C-terminal part containing a single-repeat MYB-type DNA- binding domain (Sakai et al. 1998) called a GARP domain (Riechmann et al. 2000) and the proline- and glutamine-rich...

Ngày tải lên: 14/02/2014, 12:20

... vulgare) by rubbing the virus suspension in 100 m M phosphate buffer (pH 7.2) containing 2% poly(vinylpyrrolidone) in the presence of Carborundum powder. Inoculated plants were maintained in a greenhouse ... cDNA encoding SNA; RIP, ribosome-inactivating protein; SNA, Sambucus nigra agglutinin; SNLRP, Sambucus nigra lectin-related protein; TMV, tobacco mosaic virus. Enzyme: ribosome-inactivating protein ... are located in domain 1, and two others (linking Cys147- Cys162 and Cys188-Cys205, respectively) are located in domain 2. The sugar-binding activity of SNA-If relies on two carbohydrate-binding sites...

Ngày tải lên: 31/03/2014, 23:20

... and in ux systems are very impor- tant for cellular ion relationships in natural conditions. Increasing in ux, decreasing efflux or both can maxi- mize K + uptake to maintain K + homeostasis in plants [32,33]. ... GUS activities in the transgenic seedlings or different tissues was determined after the transgenic plants had been incubated overnight at 37 °Cin1mgÆmL )1 5-bromo-4-chloro-3-indolyl-glucuronic ... Histochem- ical staining indicated that AtHELPS is mainly expressed in the young seedlings and vascular tissues of leaves and roots. Using both helps mutants and overexpression lines, we observed that, in the...

Ngày tải lên: 14/02/2014, 18:20

... con- taining DNA spots (5 lg each) corresponding to inter- nal fragments of ADC or cruzipain genes. The latter (used as a control) is a housekeeping gene that encodes the main cysteine proteinase ... to a-amanitin of the transcription of arginine decarboxylase (ADC) and cruzipain genes in transformed Trypano- soma cruzi. Permeable parasites were preincubated at 0 °C for 5 min in the absence ... bands obtained in the corresponding Southern blot assay (Fig. 3B) could only be explained by the integration, into the parasite genome, of ‡ 2 units of the plasmid pADC-8 arranged in a head-to-tail...

Ngày tải lên: 19/02/2014, 07:20

... Radding CM (1998) Interaction of human rad51 recombination protein with single-stranded DNA binding protein, RPA. Nucleic Acids Res 26, 5388–5393. 17 Stauffer ME & Chazin WJ (2004) Physical interaction between ... cloned into the intermediate vector. Constructs were introduced into Agrobacterium tumefaciens strain EHA106, and trans- formed into wild-type (Columbia) plants by floral in ltra- tion [35,36]. The transgenic ... soluble in the presence of 6 m urea. Soluble fractions were loaded on to columns contain- ing 10 mL His-Bind resin (Novagen). The columns were washed with 50 mL binding buffer containing 6 m...

Ngày tải lên: 07/03/2014, 21:20

... processes in food processing procedures (drying, smoking, curing etc.). Toldrá and Flores (1998) suggested that lipolysis is one of the main processes of lipid degradation in fresh meat during processing; ... FFA displayed a threefold increase during drying, suggesting that lipolysis occurs before or during drying, whereas polar lipids and TAG decreased in the dried reindeer meat and were significantly ... critical suggestions in the design of the experimental solar drier, and Dr. Vu Quang Thanh for instruction in indoor drying techniques and allowing me to utilize the drying machines and equipment,...

Ngày tải lên: 13/03/2014, 18:58

... that its engineering is relatively easy. 24 Transgenic Plants in opine synthesis and in the synthesis of auxin and cytokinin are in the T-DNA, while genes involved in host range and ... This book is printed on acid-free paper. Printed in Singapore by Uto-Print The Concept: Integration and Expression of Alien Genes in Transgenic Plants 11 tumefaciens stains. Several ... entering into maize nuclei. Indeed recent comparison between T-DNA integration in tobacco and in inaize suggested that the relative difficulty in maize transformation involves T-DNA integration...

Ngày tải lên: 16/03/2014, 12:20

... scFv intrabodies and assessed their binding activity to the WASP-EVH1 domain in the scFv gene-transfected T cells. Finally, we succeeded in expressing the functional scFv intra- bodies in the ... reagents for Western blotting, immunostaining, immunoprecipi- tation and blocking of protein function. Therefore, if intrabodies retain their specificity and high-affinity bind- ing properties, they ... R & Oppermann H (1988) Protein engi- neering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue pro- duced in Escherichia coli. Proc Natl Acad...

Ngày tải lên: 16/03/2014, 14:20

... contaminants. Dioxins are also formed in high-temperature chemical processes (in- cluding garbage incineration) in which organic and inorganic compounds with one or more atoms of chlorine (including molecular ... processes in metallurgy, metal working, engineering, chemical, chemico-pharmaceutical, petrochemical and other industries; and the ex- haust gases of internal combustion engines using lead-containing ... vitally important parameters determining the continuing existence of mankind. The inexorable and seemingly permanent increase of technogenic contaminants in all ecological niches creates danger...

Ngày tải lên: 16/03/2014, 18:07

... quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254. CYP2E1 causes apoptosis of cardiomyocytes in vivo W. Zhang et al. 1492 FEBS Journal ... longitudinal sections are shown in (A) (magnifi- cation ·20; scale bars = 1 mm). The hemotoxylin and eosin-stained sections of the left ventricle are shown in (B) and Masson’s trichrome stained sections ... was increased in CYP2E1 transgenic mice and cTnT R141W transgenic mice (Fig. 6), suggesting that CYP2E1 increases the apoptosis of myocytes through the mitochondrial- dependent pathway. Clinical...

Ngày tải lên: 22/03/2014, 16:20

... therefore used in the subsequent experiments aiming to investigate the increases in L- and H-chain in the cocoons. The proteins contained in the cocoons of the L ⁄ H- and L ⁄ H ⁄ IE1-lines were separated ... enhanced recombinant protein expression in the MSG cells at the level of translation, leading to a 1.5-fold increase in recombinant protein synthesis. In the present study, we generated germline trans- genic ... than that in the H-cocoons. Quantita- tive analysis of the H-chain mRNA in the MSG cells revealed that the increase in H-chain in the L⁄ H-line cocoons was not the result of a rise in the mRNA...

Ngày tải lên: 23/03/2014, 04:20

... stacking and running gel. Ur ea (6 M ) was also incorporated into the running gel. The staining for the densitometry was obtained with 0.05% Coomassie R in 25% isopropanol, 10% acetic acid in order to ... protein complexes and not free in the membrane (not shown). These r esults suggest t hat t he co-ordination o f Chl might be in part d ifferent in P SI and in PSII; PSI binds Chls both within individual ... each Lhca band both in PSI–LHCI c omplex and in recombinant samples is reported in Table 1, expressed as IOD (integrated optical density). The dens itometry allows ob taining a better repro- ducibility...

Ngày tải lên: 30/03/2014, 15:20

Ngày tải lên: 03/04/2014, 12:10

Ngày tải lên: 08/04/2014, 12:49

Ngày tải lên: 28/04/2014, 13:32

Ngày tải lên: 19/06/2014, 22:20

Ngày tải lên: 28/06/2014, 09:20