... Furthermore, minimal binding domain of BRI1 was determined using binding assays and recombinant BRI1 fragments (Kinoshita et al. 2005). In other plant species, it was also shown that mutations in BRI1 ... several of the CRF genes were down- regulated in response to salt stress. It was suggested that these genes may play an important role in mediating the input of cytokinin into the salt stress response pathway ... et al. 2002) were determined as positive regulators in ABA signaling. Gene encoding ABA-induced protein kinase 1 (PKABA1), which is a serine– threonine type protein kinase, was isolated from...
Ngày tải lên: 14/02/2014, 12:20
Molecular Farming of Antibodies in Plants
... expressed in plants. Heavy-chain derivatives are shown in gray and light-chain derivatives in pink.Solidblack lines indicate continuation of the polypeptide backbone (simple lines indicate the ... tomato include the high biomass yields (about 68,000 kg/ha, approaching the yields possible in tobacco) and the increased containment offered by growth in greenhouses. Instead of introducing transgenes ... in gray and light chains in pink.Solidblack lines indicate continuation of the polypeptide backbone (simple lines indicate the constant parts of the antibody, curly lines indicate the variable...
Ngày tải lên: 25/10/2013, 05:20
Regulating Phytonutrient Levels in Plants – Toward Modification of Plant Metabolism for Human Health
... others being delphinidin, peonidin, pelargonidin, petunidin, and malvidin. Of the flavonols, quercetin, kaempferol, myricetin, and isorhamnetin are common in fruits, quercetin being the predominant ... organisms. The methods for increasing, preventing, or redirecting flux into or within the path- way include increasing levels of a rate-limiting biosynthetic enzyme, inhibition of the activity ... third predominant flavonoid group in fruits is proanthocyanidins and their monomer units, catechins (procyanidin) or gallocate- chins (prodelphinidins). Delphinidin-derived anthocyanins are known...
Ngày tải lên: 25/10/2013, 05:20
Tài liệu Báo cáo khoa học: Shaped by the environment – adaptation in plants Meeting report based on the presentations at the FEBS Workshop ‘Adaptation Potential in Plants’ 2009 (Vienna, Austria) pdf
... gene regulons and regulatory pathways involved in freezing tolerance in Arabidopsis. Freezing tolerance increases in many plants in response to low, non-freezing temperature, a process known as ... thought by providing just such an interdisciplinary forum, in which research results, including novel concepts such as environmen- tally induced increases in mutation rates in bacteria and a ... plants carrying a camta1 ⁄ 3 double mutation are impaired in freezing tolerance [52]. These results establish a role for CAMTA proteins in cold acclima- tion and provide a possible point of integrating...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Emerging pathways in genetic Parkinson’s disease: Autosomal-recessive genes in Parkinson’s disease – a common pathway? docx
... of Parkin, PINK1, DJ-1 and HtrA2 activity remains to be determined. Parkin can be phosphorylated by a number of kinases including casein kinase 1, protein kinase A, protein kinase C [86] and cyclin-dependant ... six genes have been implicated in the pathogenesis of PD, a-synuclein, Parkin, PTEN- induced putative kinase 1 (PINK1), DJ-1, leucine-rich repeat kinase 2 (LRRK2) and ATP13A2. Mutations in the genes ... genes encoding a-synuclein, LRRK2 and ATP13A2 cause autosomal-dominant forms of parkinsonism. Mutations in the genes encoding Parkin, DJ-1 and PINK1 all cause autosomal-recessive parkinsonism of early...
Ngày tải lên: 18/02/2014, 14:20
Tài liệu Báo cáo khoa học: Cytokinin oxidase/dehydrogenase genes in barley and wheat docx
... kinins by cytokinin oxidases in plants. Plant Growth Reg. 32, 315–327. 9. Morris, R.O., Bilyeu, K.D., Laskey, J.G. & Cheikh, N. (1999) Isolation of gene enconding a glycosylated cytokinin ... modeling of the flavoprotein catabolizing plant hormones cytokinins. In Recent Research Develop ments in Proteins, vol. 2. (Pandalai, S .G., ed.), pp. 63–81. Transworld Research Network, Kerala, India. 15.Werner,T.,Motyka,V.,Strnad,M.&Schmu ¨ lling, ... gene indicates a function for cytokinins in paracrine signaling in whole tobacco plants. Plant J. 12, 401–415. 30. Emanuelsson, O., Nielsen, H., Brunak, S. & von Heijne, G. (2000) Predicting...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebrafish (Danio rerio) doc
... lgÆmL )1 proteinase K in PBS-T for 30 s to 35 min depending on the stage. They were postfixed in 4% paraformaldehyde and rinsed for 5 · 5 min in PBS-T. Prehybridization was performed in hybridization ... and larvae were incubated in preab- sorbed sheep antidigoxigenin-AP Fab fragments at 1 : 4000 dilution in blocking solution for 3 h. They were rinsed in PBS-T (6 · 15 min) and then in chromogenic buffer ... PrP family. Amino acid numbering starts from the initiator methionine. Gaps inserted to optimize alignments are indicated by dashes. Numbers in parentheses in the align- ments indicate the length...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo Y học: Studies on the nonmevalonate pathway of terpene biosynthesis The role of 2C-methyl-D -erythritol 2,4-cyclodiphosphate in plants docx
... due to long range coupling involving vicinal isoprenoid moieties. Due to the line broadening, the precision of signal integration is substan- tially reduced. However, within the experimental limits, ... the two competing deprotonation paths is illustrated in Fig. 8 for the predominant ES complex A of a sample of IPP carrying a 13 C label in its methyl group; a similar scheme involving a less stable ... known precursor of the vitamins thiamine [11] and pyridoxol [12], could be incorporated into terpenoids by E. coli [9] as well as by higher plants [7]. More specifically, plants were shown to utilize...
Ngày tải lên: 22/02/2014, 07:20
Uptake of organic chemicals in plants Human exposure assessment ppt
... variance in 141 the measured concentration in plants is explained by the variance of the 142 concentrations in soil. 143 In case of an insignificant correlation, the concentration in plant ... uptake of organic contaminants from soil by plants. The goal was to gain insight into both experimental data and predictive methods. Knowledge of uptake of con- taminants in plants is relevant for ... SPME sam- pling kinetics were studied for a number of sample types in order to (1) determine the sampling time needed to ensure equilibrium sampling, (2) select the best mode of sampling (static...
Ngày tải lên: 05/03/2014, 20:20
Báo cáo khoa học: Arabidopsis thaliana CYP77A4 is the first cytochrome P450 able to catalyze the epoxidation of free fatty acids in plants potx
... forma- tion by introducing the oxygen between carbon 9 and 10 of oleic acid before incorporation of the monomer into the cutin. In this context, it is interesting to note that inhibition studies ... characterized enzymes involved in lipid metabolism. The most simi- larly expressed genes are CYP86A8 encoding a fatty acid x-hydroxylase, a putative epoxide hydrolase, several genes encoding enzymes involved in ... represents a highly diversified set of heme-containing proteins found in bacteria, fungi, animals and plants [7]. In animals, members of the CYP4A gene subfamily mainly cata- lyze the formation of x- and...
Ngày tải lên: 07/03/2014, 03:20
Báo cáo khoa học: Phytoene synthase genes in tomato (Solanum lycopersicum L.) – new data on the structures, the deduced amino acid sequences and the expression patterns doc
... converted to violaxanthin. In plants, carotenoids are mainly involved in photosynthesis as accessory pigments, in photoprotection (quenching and xanthophylls cycle) and in the formation of abscisic ... Psy2 genes in the coding sequence region. The two genes were shown to have a highly conserved structure, with seven exons being almost identical and six introns being much more vari- able in sequence ... [23] in grass PSYs, differ- ences in the N-terminus as well as the C-terminus of the two proteins may result in differences in their plastid localization. Transcription analysis of the two genes...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Two CYP17 genes in the South African Angora goat (Capra hircus) – the identification of three genotypes that differ in copy number and steroidogenic output pptx
... also included in each assay. Fig. 6. Plasma cortisol levels in the three Angora genotypes (n = 10 per group) following intravenous insulin injection. Plasma glucose levels are shown in the inset. ... only in the genes encoding CYP17, but also in copy number. Furthermore, in vivo assays revealed that the identified genotypes differed in their ability to produce cortisol in response to intravenous ... an increase in plasma cortisol levels in response to insulin injection (Fig. 6). However, although the decrease in plasma glucose was similar for all groups, the amplitude of the response in...
Ngày tải lên: 07/03/2014, 06:20
Báo cáo khoa học: Prediction of missing enzyme genes in a bacterial metabolic network Reconstruction of the lysine-degradation pathway ofPseudomonas aeruginosa doc
... cadaverine and 1-pip- erideine, because there is no template information describing the target reaction in the current system. For EC 4.1.1.18, the list of high-scoring genes does not contain any genes ... Protein- lysine erythro-5- Hydroxylysine Protein- N6-Me-lysine Glutaryl- CoA Glutarate Glutarate semialdehyde 5-Amino pentanoate 1-Piperideine 5-Amino- pentanamide Cadaverine N2-( D-1-Carboxy- ... ethyl)- L-lysine L-2-Aminoadipate 6-semialdehyde L-2-Amino- adipate Saccharopine 6-Amino-2- oxohexanoate 6-Acetoamido- 2-oxohexanoate D-Lysine 2,5-Diamino- hexanoate 3,5-Diamino- hexanoate L-β-Lysine L-Lysine N6-Hydroxy- lysine N6-Acetyl-N6- hydroxy-lysine Aerobactin 2-Amino-5- oxohexanoate 5-Amino-3- oxohexanoate N...
Ngày tải lên: 07/03/2014, 09:20
Báo cáo khoa học: Hierarchical subfunctionalization of fabp1a, fabp1b and fabp10 tissue-specific expression may account for retention of these duplicated genes in the zebrafish (Danio rerio) genome docx
... F (2002) Insights into binding of fatty acids by fatty acid binding proteins. Mol Cell Biochem 239, 45–54. 31 Bo ¨ rchers T & Spener F (1993) Involvement of arginine in the binding of heme ... mam- malian FABP1 during zebrafish embryogenesis. The distinct patterns of expression for the fabp1b (in the intestine) and fabp10 (in the liver) genes in the devel- oping zebrafish combined represent ... acid- binding protein from bovine liver. Mol Cell Biochem 123, 23–27. 32 Thumser AE, Evans C, Worrall AF & Wilton DC (1994) Effect on ligand binding of arginine mutations in recombinant rat...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Stage specific expression of poly(malic acid)-affiliated genes in the life cycle of Physarum polycephalum Spherulin 3b and polymalatase potx
... a distinct fragmin-like actin-binding protein that controls in vivo phosphorylation of actin by the actin-fragmin kinase. Eur J Biochem 265, 240–250. 37 Donze O & Picard D (2002) RNA interference ... NKA48 contain only a single domain fold. The stability of these two proteins is highly dependent on calcium binding [25]. The typical domain motives contain a ‘tyrosine corner’ in the domain centre ... function. bc-Crystallins are two-domain proteins found in vertebrate eye lenses and have distant relatives in microorganisms (e.g. the proteins in Fig. 2). The bc-crystallin domain of spherulin 3a from...
Ngày tải lên: 07/03/2014, 12:20
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