role in dna damage response with a complex role in inhibiting or promoting hr
Báo cáo khoa học: Dual role of Nbs1 in the ataxia telangiectasia mutateddependent DNA damage response ppt
... contains a forkhead-associated (FHA) domain and a Brca1 COOH-terminus (BRCT) domain in its NH2-terminal region, as well as an Mre11-binding domain and an ATM-binding domain in its COOHterminal region ... of NBS1 in chromatin association and DNA damage responses Nucleic Acids Res 30, 4815–4822 Kobayashi J, Tauchi H, Sakamoto S, Nakamura A, Morishima K, Matsuura S, Kobayashi T, Tamai K, Tanimoto ... exogenous DNA strand breaks per cell [40] In addition to DNA damage, chromatin structure-changing molecules are able to induce rapid activation of ATM in the absence of detectable DNA strand breakage...
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Báo cáo y học: " Physical and in silico approaches identify DNA-PK in a Tax DNA-damage response interactome" pot
... defects in the cellular DNA damage repair response Results Assimilation of an interaction database for Tax We conducted a manual literature search for articles with reference to "Tax Interaction" ... ranking via our functional clustering for DNA damage proteins Clearly, DNA- PK is a critical component in cellular processes that mediate response to damage and thus the fact that our clustering ... information sources: manual extraction from Pubmed, laboratory derived physical interactions, and protein interaction databases In the first database source, the information was extracted by manually...
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Báo cáo khoa học: " Enhancement of radiosensitivity in human glioblastoma cells by the DNA N-mustard alkylating agent BO-1051 through augmented and sustained DNA damage response" potx
... right hind leg Irradiation was performed using a T-1000 Theratronic cobalt unit (Theratronic International, Inc.; Ottawa, Canada) irradiator with animals restrained in a custom jig Annexin V-PI apoptosis ... this article as: Chu et al.: Enhancement of radiosensitivity in human glioblastoma cells by the DNA N-mustard alkylating agent BO-1051 through augmented and sustained DNA damage response Radiation ... anti-gH2AX antibody was added at a dilution of 1:500 in 1% BSA and incubated overnight at 4℃ Again, the cells were washed twice in PBS before incubating in the dark for h with a FITC-labeled secondary...
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Báo cáo khoa học: Homologous desensitization of guanylyl cyclase A, the receptor for atrial natriuretic peptide, is associated with a complex phosphorylation pattern pot
... amino acids (aa), a short membrane-spanning region (21 aa) and an intracellular portion (567 aa), containing a kinase homology (KH) domain, the dimerization domain and the C-terminal catalytic ... immunoprecipitation with anti-FLAG IgG Western blot analyses with antibodies against PMCA, as a marker for the cell membrane, and extracellular signalregulated kinase ⁄ (ERK1 ⁄ 2), as a marker for the ... spectra, showed an approximately nine-fold increase in ANP-treated versus untreated samples, indicating an increase in peptide amount (sum of peak areas without pretreatment, 352 000; sum of peak areas...
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Báo cáo y học: "Functional genomics of the yeast DNA-damage response" ppsx
... Repair/recombination Adaptation Figure Various pathways by which damage to DNA can elicit a checkpoint response DNA damage may occur as a result of many different kinds of damaging agents (for ... generates single-stranded DNA, the salient intermediate in the DNA- damage checkpoint response In fact, double-strand DNA breaks can also lead to stretches of single-stranded DNA at their ends before ... of DNA damage in response to MMS in yeast, Workman and colleagues [3] focused initially on detecting the physical interactions of transcription factors with their DNA targets using the chromatin...
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Báo cáo khoa học: Characterization of the role of a trimeric protein phosphatase complex in recovery from cisplatin-induced versus noncrosslinking DNA damage potx
... case, removal from the action of the ataxia telangiectasia mutated (ATM) ⁄ ataxia telangiectasia and RAD53 related (ATR) kinases at the site of DNA damage may decrease the kinase ⁄ phosphatase ... three proteins may operate as a functional unit in vivo, playing a role in the cisplatin response [9] Cisplatin interferes with DNA function by causing intrastrand and interstrand crosslinking of ... cisplatin-induced DNA damage are consistent with Rad53p phosphorylation at a particular site being maintained by cH2AX, and when the latter is dephosphorylated, Rad53p may be dephosphorylated...
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Báo cáo Y học: Secretion of a peripheral membrane protein, MFG-E8, as a complex with membrane vesicles A possible role in membrane secretion pptx
... 5¢-ATTCTAGAG GCTAGGTTGTTGGAAAG-3¢, 5¢-ATTCTAGAGGAT GTCTTGAGCCCCTG-3¢ and 5¢-TTCTCGAGCAGGA CTGAGCATTAACAG-3¢ The two DNA fragments were ligated at the XbaI site and inserted into a cloning vector ... fetal bovine serum for 24 h, cells were washed three times with NaCl/Pi and fixed with 3% paraformaldehyde in NaCl/Pi for for extracellular staining or with methanol chilled at )20 °C for for intracellular ... domain was then amplified by PCR from the cloned plasmid with primers containing an EcoRI site at the 5¢ end (5¢-TAGAATTCCACCATGCAGGTCTCCCGT-3¢) and an EcoRV site at the 3¢ end (5¢-CAGATATCTTAACAGC...
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Báo cáo y học: "Ubiquitination of HTLV-I Tax in response to DNA damage regulates nuclear complex formation and nuclear export" pps
... shuttling in response to DNA damage may be a consequence of their inability to be ubiquitinated Lysine residues 280 and 284 are ubiquitinated in response to DNA damage Since Tax is ubiquitinated in ... how DNA damage- induced changes in the post-translational modification status of Tax affected its subcellular localization and its interactions with host cell protein In response to DNA damage, increased ... Tax occurs following DNA damage and that this modification may regulate the relocalization of Tax following DNA damage Lysine residues 280 and 284 regulate UV-induced Tax localization Ubiquitination...
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Báo cáo y học: " Vgf is a novel biomarker associated with muscle weakness in amyotrophic lateral sclerosis (ALS), with a potential role in disease pathogenesis"
... ventral horn below a lateral line across the spinal cord from the central canal Correction for tissue section thickness was made in all specimens For stereological analysis, 10 serial coronal sections ... of each individual tissue section and automatically subtracted from the values over unstained spinal cord motorneurons The optical density of cellular Vgf immunostaining in spinal cord dorsal ... ELISA assays (see Materials and Methods for more information) Decreased Vgf content In CSF and serum precedes onset of ALS-type muscle weakness assessed by rotarod-assays In our laboratory setting,...
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Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
... can be controlled by changes in DNA topology inducing formation of non-B DNA structures within the binding sites [11,12] Interactions of p53 with DNA are regulated mainly via post-translational ... cisplatin-damaged DNA: a clue to anticancer activity of cisplatin FASEB J 12, 791–799 30 Kasparkova J & Brabec V (1995) Recognition of DNA interstrand cross-links of cis-diamminedichloroplatinum(Ii) ... the cisplatin inhibitory effects could be explained by DNA damage within the p53DBS It is known that the cisplatin IACs induce considerable DNA bending and untwisting as well as perturbation of...
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Tài liệu Báo cáo khoa học: Differentiation stage-dependent preferred uptake of basolateral (systemic) glutamine into Caco-2 cells results in its accumulation in proteins with a role in cell–cell interaction pptx
... Glutamine incorporation in Caco-2 proteins determining the relative uptake of glutamine; (b) by searching for changes in the intestinal proteome; and (c) by examining glutamine incorporation into ... cytoplasmic proteins that can bind to the membrane surface in response to elevations in intracellular calcium [45] Annexin A2 is an F-actin binding protein and participates in the formation of ... carcinomas, including colon carcinoma, acting as a protection against apoptosis [57] Cytosolic NADP-dependent isocitrate dehydrogenase has a protective role against oxidative damage being a source...
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Báo cáo khoa học: The chloroplast ClpP complex in Chlamydomonas reinhardtii contains an unusual high molecular mass subunit with a large apical domain doc
... Guillardia theta (Gt, a Cryptophyte) and Thalassiosira pseudonana (Tp, a Diatom) The Viridiplantae (green, of a lighter shade for chloroplast genes) are Arabidopsis thaliana (At), Chlamydomonas ... including more bacterial sequences (available at the EMBL-Align database as ALIGN_000912) place the origin of the eukaryotic ClpP2 within Proteobacteria rather than Cyanobacteria This suggests that ... parvum, an Apicomplexan that has lost its apicoplast, also has lost both ClpP and ClpR [38,39] In any event, apicomplexan ClpP appears as an interesting target for the design of antiparasitic drugs,...
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Báo cáo khoa học: Crystal structure of the second PDZ domain of SAP97 in complex with a GluR-A C-terminal peptide ppt
... plasticity and basal GluR -A localization [10], suggesting developmental plasticity and ⁄ or existence of multiple parallel pathways in GluR -A transport To date, four PDZ domain-containing proteins have ... the two chains, and 11 (chain A) or 13 (chain B) C-terminal residues including the hexahistidinyl tags also appear unstructured The binding site region of chain B contains additional electron ... situation for non-PDZ protein interaction domains in PSD-95: an intramolecular interaction between a ‘hook’ domain and guanylate kinase domain keeps the molecule in a closed state [28,29] In conclusion,...
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Báo cáo hóa học: " Vaccination with a plasmid DNA encoding HER-2/ neu together with low doses of GM-CSF and IL-2 in patients with metastatic breast carcinoma: a pilot clinical trial" pptx
... Her2-pDNA vaccination in combination with GM-CSF and IL-2 in a small number of advanced breast cancer patients who are on concurrent trastuzumab treatment with findings warranting further exploration ... trastuzumab an attractive clinical treatment modality pDNA immunization has several advantages as compared to other vaccination strategies; while immunization with proteins primarily induces antibody ... report on administration of a Her2-pDNA vaccine in humans We demonstrate that injection of the pDNA vaccine and cytokines during concurrent trastuzumab treatment was safe, well tolerated and induced...
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báo cáo hóa học: " Boosting with intranasal dendrimeric Aβ1–15 but not Aβ1–15 peptide leads to an effective immune response following a single injection of Aβ1–40/42 in APP-tg mice" ppt
... improving cognition [4-7] Recently it has been demonstrated that non-B cell mechanisms may also have a role in clearing cerebral A [8] A multi-center A vaccine human clinical trial (AN1792) was initiated ... were harvested and thymidine incorporation determined using a liquid scintillation counter A stimulation index was calculated using the following formula: CPM of well with antigen/CPM with no antigen ... DS, Weiner HL: Nasal vaccination with a proteosome-based adjuvant and glatiramer acetate clears beta-amyloid in a mouse model of Alzheimer disease J Clin Invest 2005, 115:2423-2433 Schenk D: Amyloid-ß...
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Báo cáo khoa học: "Choosing simplified mixed models for simulations when data have a complex hierarchical organization. An example with some basic properties in Sessile oak wood (Quercus petraea Liebl.)" ppsx
... Lorraine Poor Loir-et-Cher Allier Medium Lorraine Hight forest Alsace Loir-et-Cher Lorraine Normandy Allier 2 Loir-et-Cher 1 Lorraine mod 3 Normandy modalities Loir-et-Cher Allier Poor Normandy ... several wood quality criteria (density, swelling, colour, spiral grain, multiseriate wood rays) on both standard small-size samples and industrial-size boards Our data have a hierarchical organization ... poor soils) are of particular importance for forest managers and forest policy-makers In these multiple regards, it seems important to maintain a sufficient degree of genetic variability in oak...
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Báo cáo khoa học: "Combined low initial DNA damage and high radiation-induced apoptosis confers clinical resistance to long-term toxicity in breast cancer patients treated with high-dose radiotherapy" pdf
... Every assay was made in triplicate The flow cytometry analysis was performed in a FACScalibur (Becton Dickinson, San José, CA) using a 488 nm argon laser, and each sample was analyzed in a Macintosh ... patients Radiation-induced apoptosis in breast cancer patients Initial DNA damage was determined as radiationinduced double-strand breaks (DSB) in irradiated lymphocyte from all 26 LABC patients ... patients analyzing the RIA rate by annexin V/PI staining flow cytometric analysis, defining an intrinsic individual value of radiosensitivity inherit to each patient Radiation-induced apoptosis has...
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Báo cáo y học: "Collagen-induced arthritis in C57BL/6 mice is associated with a robust and sustained T-cell response to type II collagen" pot
... (methotrexate), and a nonsteroidal anti-inflammatory drug (indomethacin) Clinical score was assessed, as a measure of spread of disease progression As expected, dexamethasone and anti-TNF mAb gave clear reductions ... Germany) and the diameter was expressed as an average for inflamed hind paws per mouse Animals were also scored for clinical signs of arthritis [13] as follows: = normal, = slight swelling and /or ... Feldmann M, Maini RN: Anti-tumor necrosis factor ameliorates joint disease in murine collagen-induced arthritis Proc Natl Acad Sci USA 1992, 89:9784-9788 Feldmann M, Maini RN: Lasker Clinical Medical...
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