regulation of sodium and potassium excretion
THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 1
... of PPARs 1.1.2 Structural domains of PPARs 1.1.3 Mechanism of action of PPARs 1.1.4 Subtypes of PPARs 1.1.5 Ligands and physiological functions of PPARα and ... PPARγ-MEDIATED REGULATION OF NHE1 68 3A.1 PPARγ AND THE EXPRESSION OF NHE1 68 3A1.1 Identification of putative PPRE on NHE1 promoter 68 3A1.2 Down -regulation of NHE1 by PPARγ ligands ... 106 and 118, leading to ligand-independent transcriptional activation of ER-response genes (Schreihofer et al., 2001) The phosphorylation of ER results in ligand-independent dimerization of ER and...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 2
... in the absence of exogenous ligands Our previous results demonstrated that both synthetic and endogenous ligands of PPARγ were capable of inducing down -regulation of PPARγ NHE1 and mRNA Among ... class of synthetic PPARγ ligands which are clinically available for treatment of type diabetes Members of TZDs include troglitazone, rosiglitazone, and pioglitazone, marketed as Rezulin, Avandia and ... Down -regulation of NHE1 by PPARγ ligands Intrigued by the presence of putative PPRE on NHE1 promoter, we next assessed the effect of PPARγ ligands on NHE1 expression both at protein level and...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 4
... effect of E2 on PPARγ-mediated down -regulation of NHE1 Since re-expression of ERα and its activation by E2 in ER negative MDA-MB231 cells rescued the inhibition of NHE1 expression by PPARγ ligands, ... PPARγ-driven transcriptional activation of PTEN (Bonofiglio et al., 2005) Based on the observation that the extent of NHE1 down -regulation of by PPARγ ligand was more pronounced in charcoal-stripped ... cells were pre-incubated with 1μM of E2 before they were exposed to 3μM of 15d-PGJ2 or 10μM of ciglitazone or 15μM of troglitazone, and the cells were next harvested and subjected to NHE1 mRNA analysis...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 6
... MCF-7, MDA-MB-231 and T47D cells were treated with different doses of synthetic ligands as well as natural ligand of PPARγ, and the protein expression and mRNA transcript level of NHE1 were assessed ... 3µM of 15d-PGJ2, suggesting a more important role of ROS/RNS in NHE1 regulation at higher concentration of PPARγ ligand The involvement of ROS/RNS in PPARγ ligandmediated down-regualtion of NHE1 ... Badawi, A F and M Z Badr (2003) "Expression of cyclooxygenase-2 and peroxisome proliferator-activated receptor-gamma and levels of prostaglandin E2 and 15deoxy-delta12,14-prostaglandin J2 in...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 3
... of colonies formed in MDA-MB231 cells, a 83% reduction in MCF-7 cells and only a 36% down -regulation in T47D cells (Figure 14D) Interesetingly, the extent of inhibition on cologenic ability of ... cells were exposed to (A) 15d-PGJ2 and (B) ciglitazone for 16h before they were washed and reseeded at a density of 1.0 X104 cells/100mm dish After 14 days of incubation, cells were stained with ... dishes) and T47D (3 X105 cells/6-well dishes) cells were treated with 15d-PGJ2, for 16h before they were washed and reseeded at a density of 1.0 X 104 cells/100mm dish After 14 days of incubation,...
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THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 5
... reverse the up -regulation of PTEN protein and 159 mRNA expression brought by PPARγ ligand, BRL49653 in MCF-7 cells (Bonofiglio et al., 2005) More recently, transcriptional up -regulation of RhoB by ... re-expression of ERα in MDA-MB-231 and its activation by estrogen present in serum blocks PPARγ-mediated down -regulation of NHE1 expression 143 Figure 22: Re-expression of ERα blocks PPARγ-mediated down -regulation ... with 3µg of reporter plasmid 3X ERE-luc and 0.3µg of renilla as described in Materials and Methods 48h after transfection, the activity of ERα was then determined using luciferase assay and the...
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Regulation of navigation and vessel-source pollution in the Northern Sea Route - Article 234 and state practice
... 15 of the Economic Zone Edict; Arts and 15 of the Environmental Edict; and Art 11 of the Decree of the Council of Ministers of the USSR, June 1990, ‘On Measures of Securing the Implementation of ... owner of a vessel registered with a state party and carrying over 2,000 tons 87 88 89 90 91 92 94 95 See Arts 1(4), and 7(4) of the NSR Regulations; Art 14 of the Economic Zone Edict; and Art of ... shall establish periods and areas closed for navigation, and also other measures ensuring the safety of shipping and the prevention, reduction, and control of pollution of the marine environment...
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Tài liệu Báo cáo khoa học: Roles of AP-2 transcription factors in the regulation of cartilage and skeletal development doc
... tumorigenesis and genetic diseases [47] Most analyses of the regulation of AP-2 and the interactions of the transcription factor with binding partners, as well as of the regulation of target gene ... treatment of the chick face with RA, and this is accompanied by an increase in apoptosis [73] The authors of this study ascribe the regulation of outgrowth of limb buds and patterning of the digits ... A.-K Wenke and A.-K Bosserhoff stage of development They found that AP-2a, AP-2b, AP-2c and AP-2e are differentially expressed during the preimplantation period, and, with the exception of AP-2a,...
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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx
... polymerases and components of the transcription machinery in the nucleus The role of actin in transcription and its regulation Actin is both a major cytoskeletal component of all eukaryotic cells and ... component of pre-mRNP particles, and is incorporated into pre-mRNAs by binding to a specific subset of RNA-binding proteins [4,22]; and (h) actin is a component of PICs and depletion of actin prevents ... actin monomers and prevents Rho-dependent nuclear accumulation of MRTF-A and SRF Actin and ABPs in transcription regulation activation [46], blocks the nuclear accumulation of MRTF-A and -B in the...
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Báo cáo khoa học: Huntington’s disease: roles of huntingtin-interacting protein 1 (HIP-1) and its molecular partner HIPPI in the regulation of apoptosis and transcription pptx
... nucleus Regulation of gene expression by HIPPI Caspase Nuclear pore complex HIP1 Fig Possible mechanisms of regulation of transcription and apoptosis by HIPPI and HIP-1 in HD Interaction of HIP-1 ... transcription caspase-6, and caspase-3 Cleavage of Bid and release of cytochrome c and apoptosis-inducing factors from the mitochondria are also observed Coexpression of wild-type htt exon and Hippi decreases ... models, brains of transgenic animals, and post-mortem brains of HD patients [2] Aggregate formation is enhanced with the increase in the number of glutamines in vitro and in vivo, and is believed...
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Báo cáo Y học: Molecular characterization of MRG19 of Saccharomyces cerevisiae Implication in the regulation of galactose and nonfermentable carbon source utilization pdf
... (Fig 10, lane and 2) and wild-type (Fig 10, lane and 4) strains bearing multiple copies of MRG19 and grown in the presence of glucose (Fig 10, lane and 3) and glycerol (Fig 10, lane and 4) It is ... Expression of Mrg19p as a function of cell density Wild-type cells transformed with multiple copies of MRG19 (lane and 3) and wild-type cells (lanes and 5) were allowed to grow to an OD600 of 0.5 and ... we monitored the rate of oxygen uptake in log and stationary phase cultures of wild-type and MRG19 disruptant cells The rate of oxygen uptake was increased in wild-type and MRG19 disruptant cells...
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Báo cáo khoa học: "Studies on sodium and potassium metabolism and subsequent influence on electrocardiogram in unilaterally adrenalectomized black Bengal goat (Capra hircus)" pptx
... esivda pu wollof dna ymotcelanerda gnimrofrep rof atakloK ,SFAUBW ,ygoloidaR & yregruS yranireteV fo tnemtrapeD fo idnaN S rD dna hsohG ikabeD rD ot dna seitilicaf yrassecen gnidivorp rof ,atakloK ... eht ,emulov doolb gnitamitse roF euqinhcet lacigrus dradnats gnisu arbetrev rebmul tsrif eht fo ssecorp esrevsnart eht otpu yllasrod gnidnetxe aera latsocarap ni demrofrep saw dnalg lanerda tfel ... doolB noitarepo ot detcejbus neht dna syad evitucesnoc rof detcelloc erew selpmas doolb dna sretemarap eht fo seulav lamron fo noitamitse rof desu yllaitini erew staog latnemirepxE esod eludehcs...
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Báo cáo y học: "α Impact of VIP and cAMP on the regulation of TNF-α and IL-10 production: implications for rheumatoid arthritis" pps
... presence of VIP (lane 2), and that the combination of VIP and rolipram activates both CREB and ATF-1 upon LPS stimulation (lane 3) α VIP fails to modulate T-cell production of IL-10, TNF-α, γ and ... mouse, the augmentation of IL-10 and downregulation of TNF-α and IFN-γ results in a Th2 cytokine profile that is dependent on cell types present, differentiation status, and the activation stimulus ... cells, and synovial cells — all of which play an important role in the pathology of RA — and compare the findings with murine VIP data already published Materials and methods Reagents Capture and...
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Báo cáo y học: "T-cell contact-dependent regulation of CC and CXC chemokine production in monocytes through differential involvement of NFκB: implications for rheumatoid arthritis" docx
... concentrations of the following: 25 ng/ml TNFα (gift from Dr W Stec, Centre of Macromolecular Studies, Lodz, Poland), 100 ng/ml IL-6 (gift from Dr P Ramage, Sandoz, Pharma Ltd., Basel, Switzerland) and ... production of high levels of chemokines in M-CSF-differentiated human monocytes (macrophages) Levels of both CC chemokines (MCP-1, MIP-1α, MIP-1β and RANTES) and CXC chemokines (IL-8, GROα and IP-10) ... RANTES and MCP-1 was unaffected (Figure 2c) This inverted pattern of utilization of NFκB for the T-cell contact-dependent induction of CC and CXC chemokines in monocytes is surprising and indicates...
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Báo cáo y học: "Composition and regulation of maternal and zygotic transcriptomes reflects species-specific reproductive mode" doc
... ranking of IGR length between chicken and human, and another for its fold change between mouse and human Comparison of fold changes of all-maternal one-to-one orthologs versus the set of all one-to-one ... Materials and methods) To compare the relative contribution of post-transcriptional regulation among different classes of maternal transcripts, we used the length of the 3' UTR as an estimate of the ... strict-zygotic, P < 10-4 and P < 10-3; the genome-wide set of all core and metazoan genes, P < 10-21 and P < 10-72) Similarity in regulatory architecture of maternal and zygotic genes across...
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Ceftriaxone-induced up-regulation of cortical and striatal GLT1 in the R6/2 model of Huntington’s disease pps
... to food and water ad libitum Both the housing and experimental use of animals followed the National Institutes of Health guidelines and were approved by the Institutional Animal Care and Use ... WTs and R6/2s indicate saline treatment, and WTc and R6/2c indicate ceftriaxone treatment N refers to number of animals per group Genotype and CAG repeat length We used PCR for genotyping and ... analyses and genotyping, and helped with data interpretation BRM participated in study design, and helped with data collection and analysis GVR conceptualized and designed the study, and revised...
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Báo cáo y học: "Regulation of apoptosis and priming of neutrophil oxidative burst by diisopropyl fluorophosphate" pps
... adding 70 μL of 2.8 mM TMB in PBS and 60 μL of mM hydrogen peroxide After minute of incubation at room temperature, the reaction was blocked with 50 μL of stop solution (500 μL of 10 mM sodium azide ... on apoptosis and priming of oxidative burst activity We report differential effects of DFP on neutrophil apoptosis and priming of oxidative burst activity, reflected in suppression of constitutive ... μg/mL) and analyzed using a BD FACS CANTO Flow Cytometer Data were analyzed using BD FACS DIVA software A minimum of 10 000 events were collected and analyzed at excitation wavelength of 488 nm and...
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Báo cáo y học: "Regulation of apoptosis and priming of neutrophil oxidative burst by diisopropyl fluorophosphate" pptx
... adding 70 μL of 2.8 mM TMB in PBS and 60 μL of mM hydrogen peroxide After minute of incubation at room temperature, the reaction was blocked with 50 μL of stop solution (500 μL of 10 mM sodium azide ... on apoptosis and priming of oxidative burst activity We report differential effects of DFP on neutrophil apoptosis and priming of oxidative burst activity, reflected in suppression of constitutive ... μg/mL) and analyzed using a BD FACS CANTO Flow Cytometer Data were analyzed using BD FACS DIVA software A minimum of 10 000 events were collected and analyzed at excitation wavelength of 488 nm and...
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Báo cáo y học: "Advances in understanding the regulation of apoptosis and mitosis by peroxisome-proliferator activated receptors in pre-clinical models: relevance for human health and disease" potx
... PPAR ligands seen in pre-clinical models of efficacy and safety in the context of human health and disease Peroxisome proliferator-activated receptors: structure, ligands, expression and target ... PPARα ligands such as Wy-14,643, ciprofibrate and clofibrate are known to produce peroxisome proliferation and liver tumors in rats and mice [75,76] However, since PP belong to the class of carcinogens ... Pennie W, Hedley K, Soames A, Kimber I and Roberts RA Downregulation of Lactoferrin by PPARalpha Ligands: Role in Perturbation of Hepatocyte Proliferation and Apoptosis Toxicol Sci 2002, 68:304-313...
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