... probe (lanes 1, 2) and binding was FEBS Journal 272 (2005) 5191–5205 ª 2005 FEBS A Parthasarthy and K P Gopinathan completely abolished when the TATATAA sequence was mutated to GATATCA (lanes 3, ... individual members from within a multigene family Gly like tRNA1 may depend on the developmental stage and the overall availability of transcription factors Gly We made a comparative analysis of ... Regulation of pol III transcription A Parthasarthy and K P Gopinathan and making them unavailable for transcription The ‘TATATAA’ sequence present in the flanking regions Gly of tRNA1 -6,7 was responsible...
... that MS lesions localize in a random fashion, more careful analysis reveals a distinct symmetry when small plaques and all demyelinating activity are taken into account The signal for contralateral ... Mogi M, Harada M, Riederer P, Narabayashi H, Fujita K, Nagatsu T: Tumor necrosis factor-alpha (TNF-alpha) increases both in the brain and in the cerebrospinal fluid from parkinsonian patients ... dexamethasone, and not mimicked by rh-TNF-alpha, IL1beta and IFN-gamma J Neurochem 2002, 81:150-157 Ariza D, Lima MM, Moreira CG, Dombrowski PA, Avila TV, Allemand A, Mendes DA, Da Cunha C, Vital...
... Park H D., Sasaki Y., Maruyama T., Yanagisawa E., Hiraishi A and Kato K (2001) Degradation of the cyanobacterial hepatotoxin microcystin bya new bacterium isolated froma hypertrophic lake Environ ... blue-green algae, Microcystis, Anabaena, Oscillatoria and in lake Kasumigaura Environ.Tech., 14, 433-442 Park H.-D., Iwami C., Watanabe M F., Harada K.-I., Okino T and Hayashi H (1998) Temporal variabilities ... Table - Primers and TaqMan Probes used Primer and Probe Sequence (5’-3’) Reference MF MR gacccgatgttcaagatact ctcctcccacaaatcaggac Saito et al., 2003b QMF QMR QMT (Probe) agacgcacgctcacctcaa...
... amplified by PCR from total DNA using the primers pADm1 (forward; 5¢-AGCAGTCGACGA AGCGACGAAGTGAAGCTGCGTGA-3¢) and pADm3 (reverse; 5¢-ATCCGTCGACATGCTTTTTAACTGTT CG-3¢) After digestion with SalI (which ... 365–377 Biswas, G., Adebanjo, O .A. , Freedman, B.D., Anandatheerthavarada, H.K., Vijayasarathy, C., Zaidi, M., Kotlikoff, M & Avadhani, N.G (1999) Retrograde Ca2+ signaling in C2C12 skeletal myocytes ... differentiated, both structurally and functionally, with highly developed cristae full of ATP synthase complexes For this reason, ATP synthase and in particular the a- F1-ATPase and b-F1-ATPase catalytic...
... following antibodies were used: anti-ADAM10, a polyclonal rabbit antibody against endogenous ADAM10 and anti-TACE, and a polyclonal rabbit antibody against endogenous TACE (Chemikon International, ... substrate CDPstar (Tropix) Emitted light was densitometrically analyzed by using a digital camera and the software AIDA 2.0 (Raytest, Straubenhardt, Germany) Isolation and detection of APPsa by ... 25–31 Takahashi, S., Kasai, K., Hatsuzawa, K., Kitamura, N., Misumi, Y., Ikehara, Y., Murakami, K & Nakayama, K (1993) A mutation of furin causes the lack of precursor-processing activity in human...
... d(5¢-AACAACGCAGCTGGGCTCTG GAACCAT), ECF -A1 41Q d(5¢-TCAACCTCTAACCAG GCTACTCCGCTG) ECM-G77Q d(5¢-AACAACGCTGG CCAGCACGCTAACCAC) and ECM-Q14 6A d(5¢-TCT ACTGCTAACGCGGATTCTCCGCTG) following the manufacturer’s ... human manganese superoxide dismutase: effects on structure, stability, and catalysis Biochemistry 39, 7131– 7137 31 Hiraoka, B.Y., Yamakura, F., Sugio, S & Nakayama, K (2000) A change of the metal-specific ... Institut Jacques Monod, Paris, France) as template DNA MnSOD was cloned using the primers ECM-5¢ d(5¢-AGCTATACCCTGCCATCCCTG) and ECM-3¢ d(5¢-TTATTTTTTCGCCGCAAAACGTG) and E coli genomic DNA as template...
... CAT GGA CGA GCT GTA CAA GTC TAA CCC AAA TTT TAC TGG G-3') and NtMMP-Cterm_rev (5'-TCT AGA TTT AAA TTA AAT GGA GAA ATG ATA AG-3') Introduced restriction sites are shown in italic The three fragments ... all recombinant forms with APMA, a metallo-organic activator of metalloproteases [16], did not enhance casein degradation, indicating that recombinant NtMMP1 is already present in an active form ... leaves Analysis of recombinant NtMMP1 produced transiently in tobacco leaves A: Immunoblot analysis of fractions from immobilized metal affinity chromatography purification of NtMMP1-apo Equal...
... 161 A book 162 A hate 163 A thank 164 A noon 165 A chorus 166 A Italy 167 A say 168 A language 169 A print 170 A English 171 A study 172 A official 173 A language 174 A English 175 A China 176 A ... 85 A rakes A briefcases A theme A hot A that B boats B oranges B there B got B make 86 87 88 89 90 A fat A name A bear A last A page B hate B fame B clear B fast B game 91 92 93 94 95 A find A ... energy 64 A export 65 A plant 66 A nuclear 67 A dollar 68 A food 69 A force 70 A earth 71 A nest 72 A sunrise 73 A worried 74 A physical 75 A advance 76 A pneumonia 77 A world 78 A meat 79 A mankind...
... medical-waste-recycling-products The community awareness of the danger of medical wastes should be raised through various ways, ranging from propaganda, education and dissemination of information ... after surgeries Also from the Ministry of public health, 2/3 of all hospitals have not applied safety waste disposal methods Most wastes have not been classified and disinfected before discharging ... dumping waste bags to roadsides In particular, Hanoi has thousands of medical stations, making up about 2% of total wastes Only 60 hospitals and medical centers have signed up for waste treatment...
... 17 AN ANALYSIS OF THE INAUGURAL ADDRESS BY G.W BUSH IN THE U.S PRESIDENTIAL ELECTION 2004 FROMA PERSPECTIVE OF D .A DATA ANALYSIS AND DISCUSSION 3.1 Analysis of data 3.1.1 Lexical choice By referring ... as sabbatical, ultimate, eventual, persistent, anew are intentionally used by the speaker for emphatic purposes Most obvious is the case of anew, which stands out in paragraphs: Today America ... Uni 16 AN ANALYSIS OF THE INAUGURAL ADDRESS BY G.W BUSH IN THE U.S PRESIDENTIAL ELECTION 2004 FROMA PERSPECTIVE OF D .A Apart from the formal salutation at the beginning and farewell at the end,...
... reason for this is that a DataReader object ties up the Connection object, and no other commands can be executed while there is an open DataReader for that Connection The following example closes ... discontinuedColPos = productsSqlDataReader.GetOrdinal("Discontinued"); You can then use these int values to get the column values from productsSqlDataReader: while (productsSqlDataReader.Read()) { Console.WriteLine(productsSqlDataReader[productIDColPos]); ... maintenance nightmare Also, hard-coding the column positions makes your programs more difficult to read There isa solution to this problem: you can call the GetOrdinal() method of your DataReader...
... 4.2.4 Headache • A headache resulting from malignant disease may arise from raised intracranial pressure due to brain metastasis or progressive incurable primary brain tumours It may also be a result ... Moscol A, Zaharia M, Zaman S, Perez Escutia MA Fractionated half3 body irradiation (HBI) for the rapid palliation of widespread, symptomatic metastatic bone disease: a randomised phase III trial ... the pain specialist in cancer pain management, which can lead to greater dialogue and liaison between oncology, specialist pain and palliative care professionals The document is accompanied by...
... TMPRSS13 mRNA is expressed in a variety of human adult tissues, and predominantly in lung, placenta, pancreas and prostate [12] HAI-1 mRNA is also highly expressed in placenta, pancreas and prostate ... D, Takahashi K, Okigaki M, Arakaki N, Nakayama H, Hirono S, Sakiyama O, Takahashi K et al (1989) Molecular cloning and sequence analysis of cDNA for human hepatocyte growth factor Biochem Biophys ... 5¢-GGATTTTCTGAATCGCACCT-3¢ for TMPRSS13 (34 cycles), and 5¢-ATGGAGGCTGCTTGG GCAACA-3¢ and 5¢-ACAGGCAGCCTCGTCGGAGG-3¢ for HAI-1 (26 cycles) The GAPDH-specific primer set, 5¢-AGGTGAAGGTCGGAGTCAAC-3¢ and...
... CGGTTCACTAAACGAGCTCTGCTGCAGaaaaaatccaaaaaaaatctaaaaaaatcttttaaaa aaccccaaaaaaatttacaaaaaaGTCGACaatgc gcattGTCGACttttttgtaaatttttttggggttttttaaaagatttttttagattttttttg gattttttCTGCAGCAGAGCTCGTTTAGTGAACCG Ccttctccccggcggttagtgctgagagtgc aaaaaatccaaaaaaaatctaaaaaaatcttttaaaaaaccccaaaaaaatttacaaaaaa ... Journal compilation ª 2008 FEBS S Ma et al (1) β-gal CMV Transcription start site C β-gal (2) ARS-β-gal CMV Transcription start site β-gal AR S 5’-aaaaaatccaaaaaaaatctaaaaaaatcttttaaaaaaccccaaaaaaatttacaaaaaa-3’ ... aaaaaatccaaaaaaaatctaaaaaaatcttttaaaaaaccccaaaaaaatttacaaaaaa Primer with NcoI site Sense ARS with PstI and SalI Antisense ARS with PstI and SalI TOP ARS Acknowledgements This work was supported by funds from a...
... I may die at either place My goods to be divided into equal parts 'of which one parte and an halfe shall remaine vnto Marion Harrison alias Marion Isebrande and the daughter of William Isebrande ... that remain must answer and bear them out: for they plead more charge other ways, saying: "I am charged already with a light horse; I am to answer in this sort, and after that matter." And it is ... private gain, evade the laws; also some good advice about draining In his chapter on "Baths and Hot Wells," Harrison says that he's tasted the water of King's Newnham well, near Coventry, and that...
... Program) Three genes annotated as TatA, TatB, and TatC homologues could be found on contig 67; TatB and TatC are adjacent genes and might form a transcriptional unit, whereas the TatA homologue is ... some clear distinctions that may account for its dual role as a Tat signal and as a membrane anchor The ISP h-region exhibits a significantly higher hydrophobicity than average Tat signal peptides ... All enzymatic measurements were performed at ambient temperature on a Hitachi U-3000 spectrophotometer (Hitachi, Tokyo, Japan) Malate dehydrogenase assay Malate dehydrogenase was used as a marker...
... zeaxanthin (A Z) with a probability AZ It is known from experimental data that these two steps reach equilibrium It means that the parameters VA and AZ must vanish In the model we have assumed a linear ... which all molecules of violaxanthin are converted to antheraxanthin and all molecules of antheraxanthin are converted to zeaxanthin at a given temperature at saturating amount of VDE and at the ... violaxanthin was deepoxidated and more zeaxanthin was formed in the monogalactosyldiacylglycerol system at the plateau stage of the reaction The same was found at °C, although at this temperature...