reaction system for in vitro dag esterification with 1 14c oleoyl coa as substrates 70
Identification and characterization of iron homeostasis related genes and HCC down regulated mitochondrial carrier protein (HDMCP), a novel liver specific uncoupling protein in human hepatocellular carcinoma (HCC
... Prevention, 10 1. 2.7 .1 Hepatitis viral vaccination, 10 1. 2.7.2 Interferon-α (IFN-α) treatment, 11 1. 2.7.3 Screening for HCC in high risk populations, 11 1. 3 Molecular pathogenesis of HCC, 11 1. 3 .1 Genetic ... disease, 1. 1.4 .1 Hepatitis, 1. 1.4.2 Fibrosis and cirrhosis, 1. 1.4.3 Liver cancer, 1. 2 Hepatocellular carcinoma (HCC), 1. 2 .1 Epidemiology, 1. 2.2 Etiology, 1. 2.2 .1 HBV infection, 1. 2.2.2 HCV infection, ... alterations in HCC, 11 1. 3.2 Epigenetic alterations in HCC, 13 1. 3.3 Differential gene expression in HCC, 13 1. 4 Objective and approaches for identifying differentially expressed genes in HCC, 14 1. 5...
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Báo cáo khoa học: Proteome analysis of a rat liver nuclear insoluble protein fraction and localization of a novel protein, ISP36, to compartments in the interchromatin space pptx
... the nucleus; D, proteins derived from contaminating organelles, namely peroxisomes; and E, novel proteins found in this study No Protein 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Heterogeneous ... P48679, P 115 16 P48679, P 115 16 Q5T2B1 P70 615 P49793 P0 911 8 Q62826 P 310 00 Q10758 Q10758 Q9JIL3 Q5BJY9 P84588 P63 018 P05784 7 513 586a P6 813 4, P60 710 P63259 Q5PQX1 P34305 Q9Z0W3 P7 016 8 42 36 34 75 23 36, ... Actin-binding protein ACF7, neural isoform Actin (a, b or c) 26 27 28 29 Lamina-associated polypeptide 1b ATP binding RNA helicase C06E 1. 10 Nuclear pore complex protein 16 0 Importin b -1 subunit...
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Báo cáo khoa học: Construction of a novel detection system for protein–protein interactions using yeast G-protein signaling pdf
... FEBS 26 41 Detection system for protein–protein interactions N Fukuda et al Table Primers used for construction of plasmids and yeast strains Primer number 10 11 12 13 14 15 16 17 18 19 20 21 22 ... sequence of yeast Gc at the Table Yeast strains used in this study Strain Genotype Reference BY47 41 MC-F1 BWG 211 8 BZG 211 8 BFG 211 8 BZFG 211 8 BFG2Z18-WT BFG2Z18-K35A BFG2Z18-I31A MATa his3D1 ura3D0 leu2D0 ... data for 10 000 cells 1 indicates BWG 211 8 (the constructed ste18D strain), and ‘2’ indicates MC-F1 (the STE18-intact strain) background signal generation was also shown for the growth inhibition...
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Báo cáo khoa học: hhLIM is a novel F-actin binding protein involved in actin cytoskeleton remodeling ppt
... cytoplasmic 95 11 1 11 7 12 0 P P 15 4 16 0 19 4 P P Zinc finger C2H2 type domain LIM zinc-binding domain Protein kinase C phosphorylation site P F1 10 41 120 15 4 19 4 aa Actin F2 GST F3 F4 F1 F2 F3 ... staining was performed as described previously [12 , 31] The cells were stained for 20 with TRITC ⁄ phalloidin (1 lgÆmL )1) in blocking solution (1% BSA and 0 .1% Triton X -10 0 in NaCl ⁄ Pi) in the dark ... domain is sufficient to interact with actin, the LIM domain was functionally destroyed by replacing cysteine with serine in either domain (mLIM1) or (mLIM2), and an in vitro GST pull-down assay was...
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Báo cáo Y học: Identification and characterization of a novel activated RhoB binding protein containing a PDZ domain whose expression is specifically modulated in thyroid cells by cAMP pot
... Narumiya, S (19 96) Rhotekin, a new putative target for Rho bearing homology to a serine/threonine kinase, PKN, and rhophilin in the rho-binding domain J Biol Chem 2 71, 13 556 13 560 15 Ishizaki, ... Lim, L (19 95) A novel serine/ threonine kinase binding the Ras-related RhoA GTPase which Ó FEBS 2002 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 A new activated RhoB binding protein modulated ... by PCR-based methods As shown in Fig 1, the human protein has 87% identity with the dog protein Between amino acids 35 and 12 2, p76RBE contains an HR1-Rho-binding domain, and between amino acids...
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Báo cáo khoa học: A novel four transmembrane spanning protein, CLP24 A hypoxically regulated cell junction protein pdf
... 2 71) A 51 1 01 1 51 2 01 2 51 3 01 3 51 4 01 4 51 5 01 5 51 6 01 6 51 11 7 01 28 7 51 45 8 01 61 8 51 78 9 01 95 9 51 111 10 01 128 10 51 145 11 01 1 61 115 1 17 8 12 01 195 12 51 211 13 01 13 51 14 01 14 51 15 01 15 51 16 01 ... 50 10 0 15 0 200 250 300 350 400 450 500 550 600 650 10 700 27 750 44 800 60 850 77 900 94 950 11 0 10 00 12 7 10 50 14 4 11 00 16 0 11 50 17 7 12 00 19 4 12 50 210 13 00 226 13 50 14 00 14 50 15 00 15 50 16 00 16 50 ... bovine serum (Invitrogen), mM L-glutamine (Invitrogen), 10 0 UÆmL )1 penicillin (Invitrogen), 10 0 lgÆmL )1 streptomycin (Invitrogen), 10 ngÆmL )1 human recombinant EGF (Invitrogen) and lgÆmL )1 hydrocortisone...
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Báo cáo y học: "Small RNA sequencing reveals miR-642a-3p as a novel adipocyte-specific microRNA and miR-30 as a key regulator of human adipogenesis" ppt
... 0 .18 -1. 57 ± 0.60 0.032 13 ,065 hsa-miR-22* 0. 41 ± 0.06 1. 27 ± 0. 31 0.034 1, 2 81 hsa-miR-30e 0.52 ± 0 .13 1. 41 ± 0.08 0.037 2,2 51 hsa-miR -18 1a 1. 66 ± 0.24 2.82 ± 0.64 0.0 41 5 ,10 1 hsa-miR -15 4* -1. 64 ... 0.008 1, 009 1, 338 hsa-miR-30c 1. 09 ± 0 .18 2.49 ± 0.33 0.009 7,848 hsa-miR-664 1. 41 ± 0. 01 1.98 ± 0. 01 0. 012 739 hsa-miR -10 b 0.56 ± 0 .15 1. 25 ± 0. 31 0. 019 3,258 hsa-miR-34a 3 .70 ± 0 .12 3.73 ± 0 .11 ... transfected with pSi-CHECK™-2 bearing Zaragosi et al Genome Biology 2 011 , 12 :R64 http://genomebiology.com/2 011 /12 /7/R64 Page of 13 hsa−mir−642a − chr 19 : 4 617 818 6 − 4 617 8282 ( + ) AD8 .1 AD8.2 AD3 .1 AD3.2...
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A novel sec7 domain containing protein BIG3 and its role in regulated secretory pathway
... phosphatidylinositol kinase PKA protein kinase A PKD Protein kinase D PLD phospholipase D PM plasma membrane PMSF phenyl-methyl-sulfonyl fluoride POMC Proopiomelanocortin PP1γ protein phosphotase γ ... proteins were identified in eukaryotic model systems and classified into subfamilies There are 15 mammalian Sec7-domaincontaining proteins assigned into two major classes and further subclasses based ... binding of a cargo protein with a motor protein dynein (Tai et al., 19 99); or mediated via adaptor proteins (Nakagawa et al., 2000), or binding to a lipid domain (Noda et al., 20 01) Protein kinase...
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A novel membrane pool of protein kinase c and its role in mammalian cell signaling
... similarity in the catalytic domain to protein kinase C186 ,18 8 The best characterized member of PRKs is PRK1, also known as PKN (protein kinase N )18 5 or PAK1 (protease-activated protein kinase 1) 183 ... protein kinase activity of PRK 118 7 In 19 96, it was found that the small GTPase RhoA binds to PRK1 in a GTP-dependent manner2 01 In addition, PRK1 can interact with RhoB and RhoC2 01 PRK1 has been ... shown in Figure1 .1 Figure 1. 1 Major members of the serine/threonine kinase family and tyrosine kinase family (adapted from Hanks S.K, et al.34) In addition to serine/threonine kinases and tyrosine...
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Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf
... calcium-binding domain of the protein (A) Schematic drawing of the recombinant proteins Six GST-fused recombinant proteins containing the three distinctive domains of tandem repeat (R1), the Glu-rich ... was incubated first in 5% fat-free dried milk in NaCl ⁄ Tris for h, and then in the same solution containing the antibodies (1 : 10 00 dilution) overnight After washing the membrane twice (10 mins ... Ca2+-binding proteins, calsequestrin, calmodulin, troponin C, and S 10 0, with the cationic carbocyanine dye ‘Stains-all’ J Biol Chem 258, 11 267– 11 273 Maruyama K, Mikawa T & Ebashi S (19 84) Detection...
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Báo cáo khoa học: Gc recruitment system incorporating a novel signal amplification circuit to screen transient protein-protein interactions pot
... BFG2Z18-I31A BFG2Z18-K35A BFG2Z18-WT BZFG 211 8 FG0 FG1 FG2 FG3 FG4 FG-955 MATa his3D1 ura3D0 leu2D0 met15D0 BY47 41 PFIG1-FIG1-EGFP ste18D::kanMX4 his3D::URA3-PSTE18-Gccyto-Fc BY47 41 PFIG1-FIG1-EGFP ... BFG2Z18-WT PHOP2::LEU2-PFIG1-Gc BZFG 211 8 PHOP2::LEU2-PFIG1-Gc BY47 41 PFIG1-FIG1-EGFP ste18D::kanMX4-PPGK1-ZEGFR,mem his3D::URA3-PSTE18-Gccyto-Fc PHOP2::LEU2-PFIG1-Gc BY47 41 PFIG1-FIG1-EGFP ste18D::kanMX4-PPGK1-HXT1 ... Z-domain with Lys35 replaced by alanine (ZK35A) (4.6 · 10 6 m )1) , the wild-type Z-domain (ZWT) (5.9 · 10 7 m )1) , and the dimer of ZWT (ZZ) (6.8 · 10 8 m )1) [17 ] With ZI31A and Fc as a model for the...
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Báo cáo khoa học: The A domain of fibronectin-binding protein B of Staphylococcus aureus contains a novel fibronectin binding site pdf
... Journal compilation ª 2 011 FEBS 2363 A domain of fibronectin-binding protein B A F M Burke et al RU 10 0 A N 80 Response 40 GBD 9 10 11 12 13 14 607 12 65 12 66 19 08 V 15 10 11 12 C 19 18–2477 B rFnBPB309–480 ... the binding sites for the A domain of FnBPB in Fn A domain of fibronectin-binding protein B 10 0 80 60 40 20 0 10 15 20 Cation conc (mM) 25 30 Fig Inhibition of rFnBPB163–480 binding to fibrinogen ... mechanisms are involved in the binding of the A domain of FnBPB to the two ligands A domain of fibronectin-binding protein B rFnBPB163–480 WT 30 20 10 rFnBPB163–480 N 312 A/F 314 A 10 –20 –30 50 10 0 15 0 200...
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Báo cáo khóa học: SUT2 is a novel multicopy suppressor of low activity of the cAMP/protein kinase A pathway in yeast docx
... 2 71) 12 85 Table S cerevisiae strains and plasmids used in this study Characteristics Yeast strains MB1 TG1 MR349 AR1 YHUM 214 YHUM 216 MR1 61 MR 211 MR298 AR2 MR287 AR3 AR4 Source CEN.PK2; MATa ras2::kanMX ... protein Cells were fixed in 70% ethanol for DAPI staining and imaged as described in Experimental procedures Fig In uence of Sut2p on invasive growth, FLO 11 expression and Ras protein level in R1278b ... Dras1 Dras2 the Dras2 strain was transformed with a low copy URA3selectable plasmid construct encoding RAS2, prior to deletion of RAS1 In addition, this mutant strain carried SUT2 on a LEU2 plasmid...
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
... Trp222 Trp222 Arg 213 Arg 213 Trp92 Asp 212 Trp92 Asp 212 Gly 91 His290 Gly 91 His290 Gly90 Gly90 Ser162 Tyr190 Ser162 Tyr190 Tyr190 Asp1 61 Tyr292 Tyr190 Gly89 Gly2 91 Gly89 Gly2 91 His88 Asp1 61 His88 Tyr292 ... Triacetine Tripropionin Tributyrin Trioctanoin oil Acetylcholine ( 315 ) (80) (20) – (300) (9.28) (9 .1) – – (33) Enzyme Rv1399c 610 Acetylcholinesterase 970 Pig liver esterase 320 16 18 210 300 11 23 470 ... Biochem 2 71) 3959 α4 A C α5 Asp 260 β8 α6 His 290 Ser 16 2 α7 β7 α8 1 β6 α3 β5 β3 β4 β2 1 α2 Rv1399c Ag85c Fusarium solani pisi cutinase Bacillus subtilis lipase A B Asp 214 Asp 214 Phe 219 Phe 219 Trp222...
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Báo cáo khoa học: Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role docx
... Drosophila, encodes a protein with zinc-binding domains Proc Natl Acad Sci USA 87, 211 2– 211 6 14 Tripoulas N, LaJeunesse D, Gildea J & Shearn A (19 96) The Drosophila ash1 gene product, which is ... changes in chromatin composition [19 ,20] As DIDO1 associates with chromatin in general, and not only in centromeric regions, chromatin instability is the most probable explanation for the lagging ... simultaneously to maintain transcription The PHF3 protein was recovered in initial analyses and also contains a TFS2M domain showing the same architecture as the DIDO1 long isoform, although lacking the...
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Báo cáo khoa học: The 3¢-UTR of the mRNA coding for the major protein kinase C substrate MARCKS contains a novel CU-rich element interacting with the mRNA stabilizing factors HuD and HuR ppt
... ARE-containing mRNAs [68] and has been associated with the activation of c-Jun N-terminal kinase (JNK) [69] Similarly, activation of MAP kinase-activated protein kinase has been associated with the ... Physiol 279, 16 11 16 20 Blackshear, P.J (19 93) The MARCKS family of cellular protein kinase C substrates J Biol Chem 268, 15 01 15 04 Aderem, A (19 95) The MARCKS family of protein kinase C substrates ... 465– 470 11 Antic, D & Keene, J (19 98) Messenger ribonucleoprotein complexes containing human ELAV proteins: interactions with cytoskeleton and translational apparatus J Cell Sci 11 1, 18 3 19 7 12 ...
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Báo cáo khoa học: High levels of structural disorder in scaffold proteins as exemplified by a novel neuronal protein, CASK-interactive protein1 pot
... clones obtained Clone (12 ) (2) (1) (1) (2) (1) (1) (1) (1) 10 (7) 11 (12 ) Protein Function Abl-interactor-2 (Abi2) CASK EphA2 L1CAM Myosin IB Nck1 Neurexin Adaptor protein Scaffold protein Receptor ... (MI:0 018 ) MINT -703 4 617 : Caskin1 (uniprotkb:Q8VHK2) physically interacts (MI:0 915 ) with CASK (uniprotkb:P07498) by two hybrid (MI:0 018 ) MINT -703 4748: Caskin1 (uniprotkb:Q8VHK2) physically interacts ... disorder in scaffold proteins l l l l l l l l l MINT -703 4579: Caskin1 (uniprotkb:Q8VHK2) physically interacts (MI:0 915 ) with ABI2 (uniprotkb:Q9NYB9) by two hybrid (MI:0 018 ) MINT -703 4720: Caskin1 (uniprotkb:Q8VHK2)...
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Báo cáo khoa học: Identification and functional characterization of a novel barnacle cement protein pptx
... in ASW, respectively DRUs after each washing process were as follows: first loading on Au in ASW, 11 74 RU; second loading on Au in ASW, 11 77 RU; third loading on Au in ASW, 11 82 RU; first loading ... corresponding to each single peptide with molecular masses of 311 2.36 Da (Gly19Lys 51, predicted molecular mass, 311 1.47 Da) and 13 98.6 Da (Ala1-Lys14, predicted molecular mass, 13 97 .70 Da) This ... 10 .00 8.00 18 .00 9.00 4.00 27.00 1. 00 1. 00 21. 00 18 .00 4.00 2.00 0.00 14 .00 0.00 17 .00 5.00 10 .00 4.00 0.00 14 .8c – 19 .30 11 .2d – 29.60 1. 10 2.30 21. 20 18 .20 4.90 ND 0.60 14 .60 0.00 14 .60 5.20...
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Báo cáo khoa học: Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4) potx
... Pi for 20 min, quenched in 50 mm NH4Cl in NaCl ⁄ Pi for 15 min, and permeabilized in 0 .1% (w ⁄ v) Triton X -10 0 in NaCl ⁄ Pi for After blocking with 0 .1% (w ⁄ v) gelatin in NaCl ⁄ Pi for 30 min, ... [a Bro1 domain-containing protein with a thioester-linkage site of isoprenoid lipid (CAAX motif, C standing for cysteine, A for generally aliphatic amino acid, and X for any amino acid)] in this ... for testing protein prenylation in vitro and in vivo BMC Biochem 7, doi :10 .11 86 /14 712 0 91- 7-6 Winter-Vann AM & Casey PJ (2005) Post-prenylationprocessing enzymes as new targets in oncogenesis Nat...
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Báo cáo khóa học: Functional properties of the protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus A member of a novel protein family related to protein disulfide-isomerase doc
... Reduced/denatured 3 :1 C146 C146 – C149 9 :1 C146 /14 9 C146 /14 9 C149 C149/38 1: 1 C146 C146 C149 C149 3 :1 C146 /14 9 C146 /14 9 C149/35 C149 9 :1 C146 /14 9/35 C146 /14 9/38 C149/35 C149/38 for all the proteins The spectra ... Rowling, P.J & Freedman, R.B (19 92) Folding in vitro of bovine pancreatic trypsin inhibitor in the presence of proteins of the endoplasmic reticulum Proteins 14 , 10 15 43 Robb, F.T., Maeder, D.L., ... quencher Fluorescence quenching was used as the basis for monitoring the disulfide bond-forming activity of PfPDO Spectrofluorimetric analysis The assay was performed in McIlvaine buffer (0.2 M disodium...
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